Draft genome sequence of strain HYG9370 isolated from Japanese Black Cattle in Japan.

Here, we report the draft genome sequence of strain HYG9370 (ID: JD563) isolated from a clinically healthy Japanese Black steer. The genome information of this strain provides valuable insights into the genetic diversity of , which has been isolated from multiple hosts and environments.

Whole-genome sequencing of live attenuated bovine adenovirus type 7 vaccine strain TS-GT suggests biomarkers for virulence attenuation.

Bovine adenovirus type 7 (BAdV-7) is one of the most important respiratory and enteric pathogens in the cattle industry. Although live attenuated vaccines are used to control the virus in Japan, limited information is available on the genomic regions that determine viral pathogenicity. We determined the complete genome sequence of the attenuated BAdV-7 strain TS-GT.

Complete Genome Sequence of Bovine Adenovirus Type 7 Strain Fukuroi, Isolated from a Cow with Respiratory Disease.

We determined the complete genome sequence of the bovine adenovirus type 7 prototype strain Fukuroi using next-generation sequencing technology. We found that the viral genome is 30,034 bp long and has the shortest inverted terminal repeats among known adenoviruses.

An outbreak of duck hepatitis A virus type 1 infection in Japan.

In June 2015, a highly fatal and acute disease broke out in a duckling farm in Hyogo Prefecture, Japan. The birds exhibited poor growth, reduced movement, lying in a dorsal recumbent position, depression, lethargy, ataxia and opisthotonus, with a high mortality rate of approximately 76%. By performing a reverse transcription-polymerase chain reaction (RT-PCR) using primers specific for duck hepatitis A virus type 1 (DHAV-1), we obtained the PCR products of a predicted size.

Mitochondrial inclusion bodies (intracytoplasmic acidophilic droplets) in neurons of chicken spinal cords increase with age.

We studied the pathologic features of neurons that contain intracytoplasmic acidophilic droplets (IADs) in chicken spinal cords. The IADs were lustrous spheroid bodies scattered in the cytoplasm of neurons, variable in size, and protein-rich bodies stained eosinophilic with hematoxylin-eosin, acidophilic with Azan, blue indigo with phosphotungstic acid hematoxylin, and yellow-green with Elastica van Gieson stain histopathologically. Ultrastructurally, almost all IADs were observed as homogeneous highly electron-dense spheroid bodies enclosed by double-limited membranes.

Henhouse feeding style and Salmonella Enteritidis contamination in unvaccinated flocks of egg farms, April 1994-March 2001.

From April 1994 to March 2001, monitoring tests (surveillance) for contamination of Salmonella Enteritidis (SE) at commercial egg farms and farm traceback inspections for cases of SE foodborne illness were carried out in the Himeji Livestock Hygiene Service Center jurisdiction of Hyogo Prefecture, Japan. SE vaccination was not performed before the outbreak of SE-associated foodborne illness, and SE contamination of a farm was recognized. In the surveillance, the year average percentages of identified SE-positive farms were 6.

Logistical study in Hyogo prefecture on disposal of poultry carcasses infected with highly pathogenic avian influenza virus to prevent infection spreading to other flocks.

Establishment of a disposal plan for carcasses in advance is important for prevention of epidemics. A disposal plan for contaminated goods such as poultry carcasses infected with highly pathogenic avian influenza (HPAI) virus was studied in Hyogo Prefecture, Japan. We investigated all poultry farms with over 1,000 birds for their locations, species and numbers of birds, structure and size of poultry facilities and land spaces of the farms.

Plasma cell myeloma producing IgG, IgM, and IgA immunoglobulins in a cow.

A diagnosis of plasma cell myeloma was made in a 6-year-old Holstein cow that showed continuous nosebleed, progressing emaciation, and hyperglobulinemia. Necropsy revealed enlargement of the caudal mesenteric lymph nodes. Lymph nodes and spleen were infiltrated by neoplastic plasma cells.

Melanotic neurofibroma in a steer.

A melanotic neurofibroma in a steer was investigated histologically, immunohistochemically and ultrastructurally. A very large tumor mass was located in the region of the head and right cheek. The tumor tissue consisted of an admixture of cells resembling Schwann cells and spindle-shaped cells, and they frequently contained melanin granules.

Disseminated intravascular coagulation in chickens inoculated with Erysipelothrix rhusiopathiae.

In a first experiment, 28 specific pathogen-free chickens aged 3 weeks showed clinical signs 1 to 5 days after intramuscular inoculation with Erysipelothrix rhusiopathiae. Twelve of 28 birds died 2 to 4 days after inoculation. Macroscopically, the liver, spleen and kidneys were seen to be enlarged and congested.