MAGs-centric crack: how long will, spore-positive and most , microsymbionts remain recalcitrant to axenic growth?

Nearly 50 years after the ground-breaking isolation of the primary microsymbiont under axenic conditions, efforts to isolate a substantial number of and strains continue with enduring challenges and complexities. This study aimed to streamline genomic insights through comparative and predictive tools to extract traits crucial for isolating specific in axenic conditions. Pangenome analysis unveiled significant genetic diversity, suggesting untapped potential for cultivation strategies.

Behaviour of Tunisian fed high-calorie diets: biochemical disturbance and histopathological alterations.

This work investigated the biochemical disturbances and histological alteration in animal model fed different high calorie diets (HCDs) during three months. Four diets were used: a low-calorie natural diet, halophyte plant used as control (LCD), a high standard carbohydrate diet rich in protein, HCD 0, a high carbohydrate diet rich in two concentrations of fat, HCD 1 and HCD 2. All animals having received HCDs developed dyslipidemia after one month of experiment with distinction of different sub-groups developing or not obesity and diabetes.

Reformulation of Tunisian Sun-Dried Merguez with Camel Meat: Characterization of Physicochemical and Compositional Changes in Organic Acids, Fatty Acids, Volatile Compounds, and Minerals.

Traditional sun-dried merguez is an authentic Tunisian dried sausage made with a large number of spices and herbs, which was reformulated in this study with camel meat and hump fat and dried as in the artisanal process. This research studied the physicochemical, microbiological, and chemical compositional changes that occurred in fresh camel merguez (FCM) after 12 days of drying to achieve traditional dried camel merguez (DCM). The results showed significant weight loss (54.

Probiotic consortium modulating the gut microbiota composition and function of sterile Mediterranean fruit flies.

The sterile insect technique (SIT) remains a successful approach in managing pest insects. However, the long-term mass rearing and sterilizing radiation associated with SIT have been observed to induce physiological and ecological fitness decline in target insects. This decline may be attributed to various factors, including commensal microbiota dysbiosis, selection procedures, loss of heterozygosity, and other complex interactions.

Genetic diversity of Hyalomma marginatum in Tunisia is not influenced by the bio-climate.

Ticks are important ectoparasites responsible for the transmission of several pathogens with significant medical, veterinary, and economic impacts. Climate and social changes have generated substantial changes in ticks' distribution, abundance, and activity patterns, including ticks belonging to the Hyalomma marginatum species. Knowledge on the genetic structure and dynamics of H.

Effect on Mill. fruit powders embedded on physicochemical properties, biological activities, and rheologic quality of cake.

This study aimed to improve the effects on the physical, textural, and rheological characteristics of cake supplied with fruit powder at rates of 0%, 3%, 5%, and 10%. The Physicochemical and antioxidant as well as the antibacterial activity and sensory qualities of fruits were also investigated. The highest levels reached the values of 245.

Influence of Fermentation Container Type on Chemical and Microbiological Parameters of Spontaneously Fermented Cow and Goat Milk.

Fermented goat milk is an artisanal beverage with excellent nutritional properties. There are limited data on its physicochemical properties, fatty acids, phenolic acids, and on any insight on microbiota. The aim of this research was to conduct a pilot study to compare these parameters in raw cow and goat milk before and after spontaneous fermentation in a clay pot and glass container at 37 °C for 24 h.

Diversity and adaptation properties of actinobacteria associated with Tunisian stone ruins.

Stone surface is a unique biological niche that may host a rich microbial diversity. The exploration of the biodiversity of the stone microbiome represents a major challenge and an opportunity to characterize new strains equipped with valuable biological activity. Here, we explored the diversity and adaptation strategies of total bacterial communities associated with Roman stone ruins in Tunisia by considering the effects of geo-climatic regions and stone geochemistry.

Heavy Metal Bioaccumulation and Oxidative Stress Profile in Brachidontes pharaonis (Bivalvia: Mytilidae) from the Tunisian Coast: Insight into Its Relevance as Bioindicator of Marine Pollution.

This study aims to verify the relevance of Brachidontes pharaonis to assess the ecotoxicological status of polluted sites. For this, the levels of some heavy metals (i.e.

Glyphosate exposure modulates lipid composition, histo-architecture and oxidative stress status and induces neurotoxicity in the smooth scallop Flexopecten glaber.

Glyphosate is the most sprayed pesticide across the globe. Its toxicity to non-target marine organisms has recently piqued the scientific community's interest. Therefore, the purpose of this study is to investigate the potentially toxic effects of glyphosate on scallops, an ecologically and economically important bivalve group.

Assessment of the heavy metal levels and biomarker responses in the smooth scallop Flexopecten glaber from a heavily urbanized Mediterranean lagoon (Bizerte lagoon).

Marine heavy metal pollution is a worldwide serious issue. Like almost all Mediterranean lagoons, the Bizerte lagoon is highly urbanized and suffers from intensive anthropogenic pressure. In the present study, we screened the metal contamination and biomarker responses in the smooth scallop Flexopecten glaber inhabiting this vulnerable ecosystem.

Polar and neutral lipid composition of the copepod Lernaeocera lusci and its host Merluccius merluccius in relationship with the parasite intensity.

Parasitic copepod Lernaeocera lusci is a common mesoparasite of the hake Merluccius merluccius. Although widely distributed throughout the Mediterranean, little is known about this pathogen. The current study was designed to assess the impact of different L.

Assessment of the impacts of glyphosate and its commercial formulation Roundup® on the respiratory tree of the sea cucumber Holothuria forskali using a multivariate biomarker approach.

In this study, the potential hazardous impacts of the technical grade glyphosate acid (GLY) and its commercial formulation roundup (RD®) were evaluated for the first time on holothurians. To do this, redox status, fatty acid (FA) profile, and histopathology aspects were assessed in the respiratory tree tissue of the sea cucumber Holothuria forskali following short-term exposure (96 h) to a series of concentrations (10, 100 and 1000 μg L) of GLY and RD® (glyphosate acid equivalent). Our results showed that both GLY and RD® promoted oxidative stress highlighted with an increase of hydrogen peroxide (HO), malondialdehyde (MDA), lipid peroxides (LOOH) and advanced oxidation protein product (AOPP) levels in all treated groups.

African Pharmacogenomics Consortium: Consolidating pharmacogenomics knowledge, capacity development and translation in Africa: Consolidating pharmacogenomics knowledge, capacity development and translation in Africa.

The African Pharmacogenomics Consortium (APC) was formally launched on the 6th September 2018. This white paper outlines its vision, and objectives towards addressing challenges of conducting and applying pharmacogenomics in Africa and identifies opportunities for advancement of individualized drugs use on the continent.  Africa, especially south of the Sahara, is beset with a huge burden of infectious diseases with much co-morbidity whose multiplicity and intersection are major challenges in achieving the sustainable development goals (SDG), SDG3, on health and wellness.

Complete Genome Sequence of a New Strain of Sida Golden Mosaic Buckup Virus from Florida, USA.

The complete genome sequence of a bipartite begomovirus found in a sp. plant growing in Bradenton, FL, was determined. The virus is a new strain of Sida golden mosaic Buckup virus (family , genus ).

Mercury disrupts redox status, up-regulates metallothionein and induces genotoxicity in respiratory tree of sea cucumber ().

Mercury (Hg) is among the most deleterious contaminant in the aquatic environment and presents a serious risk to humans and ecosystems. This study evaluated the effects of Hg on oxidative stress biomarkers, DNA integrity and histological structure of the respiratory tree of exposed to different concentrations of mercury chloride HgCl (0.04, 0.

Effects of acute mercury exposure on fatty acid composition and oxidative stress biomarkers in Holothuria forskali body wall.

Mercury is one of the most harmful pollutant that threat marine biota. This study assessed the Hg impact on the fatty acid (FA) composition and the antioxidant statues in Holothuria forskali body wall tissue. Specimens were exposed to HgCl graded doses (40, 80 and 160 μg L) for 96 h.

Comparison of genus-specific primers in RT-PCR for the broad-spectrum detection of viruses in the genus Potyvirus by plant diagnostic laboratories.

The Potyvirus genus is one of the largest genera of plant viruses and encompasses many economically important pathogens. While a number of degenerate primers for use in broad spectrum RT-PCR assays have been published, it is not clear which of these primers would be the most useful for use by plant diagnostic laboratories. Twelve sets of primers were tested for their ability to detect nine potyviruses in a two-step RT-PCR.

Electroretinographic evidence suggesting that the type 2 diabetic retinopathy of the sand rat Psammomys obesus is comparable to that of humans.

Purpose: Type 2 diabetic retinopathy is the main cause of acquired blindness in adults. The aim of this work was to examine the retinal function of the sand rat Psammomys obesus as an animal model of diet-induced type 2 diabetes when subjected to a hypercaloric regimen.

Materials And Methods: Hyperglycemia was induced in Psammomys obesus by high caloric diet (4 kcal/g).