Benchmark dose analysis for PAHs hydroxyl metabolites in urine based on mitochondrial damage of peripheral blood leucocytes in coke oven workers in China.

Objectives: The aim was to explore the dose-response relationship between occupational polycyclic aromatic hydrocarbons (PAHs) exposure and mitochondrial damage in coke oven plants workers.

Methods: 544 workers and 238 healthy people were recruited. The ultra-high performance liquid chromatography was used to determine the level of 1-hydroxypyrene, 1-hydroxynaphthalene, 2-hydroxynaphthalene and 3-hydroxyphenanthrene.

[Interaction of shift work and psychological capital on abnormal glucose and lipid metabolism among natural gas field workers].

To explore the interaction between shift work and psychological capital on abnormal glucose and lipid metabolism. A convenient sampling survey of demographics characteristics, shift work and psychological capital was conducted on 1 415 natural gas field workers by questionnaire in October 2018,and their physiological and biochemical indexes were measured according to standard norms. Multivariate logistic regression model was used to analyze the interaction between shift work and psychological capital on abnormal glucose and lipid metabolism.

[Correlation of job monotony and social support on daily hassle: a cross-sectional study based on natural gas field workers].

To explore the correlation of job monotony and social support on daily hassle. A convenient sampling survey of demographics characteristics, job monotony, social support and daily hassle was conducted on 1 466 natural gas field workers by questionnaire in October; 2018. Multivariate logistic regression model was used to analyze the correlation of job monotony and social support on daily hassle.

[Association between PON2 gene polymorphisms and susceptibility to noise-induced hearing loss].

To investigate the relationship between SNP and noise-induced hearing loss (NIHL) susceptibility in occupational noise exposure population in China. From 6297 for a certain steel works in contact with noise, contact length of 3 years or more and workplace noise exposure intensity of 80 dB (A) , ears or high frequency (3 000, 4 000, 6 000 Hz) average of hearing acuity 40 dB (HL) , or high frequency loss in both ears, on the basis of single whisper frequency (500, 1, 000, 2 000 Hz) average threshold of 26 dB (HL) or object as case group. A case-control study was designed with 1:1 matching.

[Ion Chromatography Method of Four Carboxylic Acids in Workplace Air].

To assess psychological acceptance and occupational stress of medical staff, analyze the relationship among personality, psychological acceptance and occupational stress and discuss the direct or indirect effects of personality to occupational stress. The gaseous four kinds of carboxylic acids in the workplace air were simultaneously collected by silica gel tube, and then desorbed by deionized water and eluted by ion chromatograph. The the content was detected by conductivity detector.

Correction: Up-regulation of exosomal miR-125a in pneumoconiosis inhibits lung cancer development by suppressing expressions of EZH2 and hnRNPK.

[This corrects the article DOI: 10.1039/C8RA03081B.].

[Determination of p-Anisidine in workplace air by HPLC].

To establish an HPLC method for determining p-Anisidine in workplace air. An C(18) capillary column was used with the detection wavelength of 240 nm, the methanol solution (v/v=62:38) as mobile phase, the flow rate was 0.9 ml/min, and the injection volume was 20 μl.

Up-regulation of exosomal miR-125a in pneumoconiosis inhibits lung cancer development by suppressing expressions of EZH2 and hnRNPK.

Exposure to nanoparticles may lead to pneumoconiosis and lung cancer; however, whether patients suffering from pneumoconiosis also face a high risk of lung cancer has been under debate for decades. Recently, exosomes have been found to play critical roles in many diseases intercellular cargo transportation, which has provided a new insight into the mechanistic investigation of nanoparticle-induced respiratory disorders. Herein, we isolated exosomes from the venous blood of patients with pneumoconiosis and healthy controls and then, we profiled the expression signatures of exosomal miRNAs using high-throughput sequencing technology.

Exosomal miRNA Profiling to Identify Nanoparticle Phagocytic Mechanisms.

Inhaling a dangerous amount of nanoparticles leads to pulmonary inflammatory and immune disorders, which integrates several kinds of cells. Exosomes are suggested to play a crucial role in intercellular communication via miRNA transmission. To investigate the role of exosomal miRNA in nanoparticle phagocytosis, a total of 54 pneumoconiosis patients along with 100 healthy controls are recruited, exosomes derived from their venous blood are collected, and then exosomal miRNAs are profiled with high-throughput sequencing technology.

Long non-coding RNA deep sequencing reveals the role of macrophage in liver disorders.

Liver disorders such as hepatitis, cirrhosis and hepatocellular carcinoma are a series of the most life threatening diseases along with extensive inflammatory cellular infiltrations. Macrophage has been proved to be key regulators and initiators of inflammation, and long non-coding RNAs (lncRNAs) are recommended to play critical roles in the occurrence and development of a variety of diseases. To uncover the role of macrophage in liver disorders via lncRNA sequencing method, we first applied a lncRNA classification pipeline to identify 1247 lncRNAs represented on the Affymetrix Mouse Genome 430/430A 2.

Serum protein expression profiling and bioinformatics analysis in workers occupationally exposed to chromium (VI).

Cr(VI) is widely-recognized as occupational and environmental contaminant, but the precise underlying mechanisms of Cr(VI) induced carcinogenic toxicity remain to be elucidated. Among kinds of toxic mechanisms, alteration of protein profiling usually elaborate a key mechanism of Cr(VI) induced toxicity and carcinogenesis. Large-scale proteins changes can reflect the onset or progression of carcinogenic toxicity, and potential serum protein biomarkers of Cr(VI) exposure.

[Association between single nucleotide polymorphismsin human heat shock protein 70 gene and susceptibility to noise-induced hearing loss].

To investigate the association between the single nucleotide polymorphisms (SNPs) at rs1043618, rs2075800, and rs2763979 in human heat shock protein 70 (HSP70) gene and susceptibility to noiseinduced hearing loss (NIHL) . A case-control study was performed, and 5 934 workers exposed to noise in an iron and steel plant in Henan, China, who underwent physical examination from 2006 to 2015, were enrolled as study subjects. According to the criteria of binaural average high - frequency (3000, 4000, and 6000 Hz) hearing threshold≥40 dB (HL) and monauralaverage speechfrequency (500, 1000, 2000 Hz) hearing threshold≥26 dB (HL) on the basis of binauralhigh frequency loss measured by pure tone audiometry, as well as the exclusion of NIHL, a total of 286 workers were enrolled as hearing loss group; after the adjustment for sex, type of work, age (difference≤5 years) , and working years of noise exposure (difference ≤2 years) , 286 workers were enrolled as control group.

Detecting the polymorphism of TERF1 gene by an improved PCR-RFLP method.

Background: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is a common and mature method of detecting the single nucleotide polymorphism (SNP). But, for the polymorphism site rs3863242 of telomeric repeat binding factor 1(TERF1) gene, there is no appropriate restriction enzyme to recognize it, which limits the research between the variants of rs3863242 and human diseases.

Methods: The reverse primer was designed based on turning the 3rd base T into the mismatch base G.

[A cohort study on occupational noise induced hearing loss in workers at an iron and steel plant].

To analyze the incidence rate of occupational noise-induced hearing loss in noise-exposed workers in an iron and steel plant from 2006 to 2015. Using a cohort study method, workers exposed to occupational noise from Jan 1, 2006 to Dec 12, 2015 were followed up and the pure tone hearing test was conducted. In total, 6 297 subjects completed two or more physical checks and the pure tone hearing test and were included in the analysis.

[Determination of 1-hydroxy-2-butanone in urine by gas chromatography].

To establish a method for the determination of 1-hydroxy-2-butanone in urine by gas chromatography. Urine samples were acidified with hydrochloric acid, and then stored in the refrigerator . After thawing under natural conditions, urine volume of 2.

[Influence of occupational stress and 5-hydroxytryptamine 2A receptor gene polymorphisms on depression in workers in a thermal power plant].

To investigate the association of occupational stress and 5-hydroxytryptamine 2A (5-HT2A) receptor gene polymorphisms with depression. In November 2010, cluster sampling was used to select 589 workers in a thermal power plant as study subjects. Questionnaires were used to investigate demographic features and occupational stressors.

Screening and Preliminary Verification of a Phage Display Single-Chain Antibody Library Against Coal Workers' Pneumoconiosis.

Objectives: To construct a phage display human antibody library (PDHAL) against pneumoconiosis for the diagnosis and treatment of coal worker pneumoconiosis (CWP).

Methods: The PDHAL was established via CWP blood and six positive antibodies were discovered. 867 coal workers (558 CWP and 309 without CWP) and 393 controls were recruited to validate the results.

Increased levels of urinary biomarkers of lipid peroxidation products among workers occupationally exposed to diesel engine exhaust.

Diesel engine exhaust (DEE) was found to induce lipid peroxidation (LPO) in animal exposure studies. LPO is a class of oxidative stress and can be reflected by detecting the levels of its production, such as malondialdehyde (MDA) and 4-hydroxynonenal (4-HNE), and etheno-DNA adducts including 1,N(6)-etheno-2'-deoxyadenosine (ɛdA) and 3,N(4)-etheno-2'-deoxycytidine (ɛdC). However, the impact of DEE exposure on LPO has not been explored in humans.

[Factors influencing electrocardiogram results in workers exposed to noise in steel-making and steel-rolling workshops of an iron and steel plant].

Objective: To investigate the factors influencing the electrocardiogram results in the workers exposed to noise in steel-making and steel rolling workshops of an iron and steel plant.

Methods: From September to December, 2013, cluster sampling was used to select 3 150 workers exposed to noise in the steel-making and steel-rolling workshops of an iron and steel plant, and a questionnaire survey and physical examinations were performed. The number of valid workers was 2 915, consisting of 1 606 workers in the steel-rolling workshop and 1 309 in the steel-making workshop.

[Correlation of occupational stress with serum levels of immunoglobulins and complement in police].

Objective: To investigate the correlation of occupational stress with the serum levels of immunoglobulins(IgG, IgM, and IgA) and complement(C3 and C4).

Methods: In May 2011, convenience sampling and cluster sampling were used to select 225 policemen from a local police station as study subjects. A questionnaire was used to investigate demographic features and occupational stress, and the immunoturbidimetric assay was applied to measure the serum levels of IgG, IgM, IgA, and complement C3 and C4.

[Effects of occupational stress and related factors on depression symptoms in train drivers].

Objective: To explore the influence of occupational stress and related factors on depression symptoms in train drives.

Methods: In March 2012, by using cluster sampling method, a cross-sectional epidemiological study was conducted in 1 402 train drivers in China. Questionnaires was investigation was conducted by face to face interview.

[Relationship between occupational stressors and serum levels of thyroid hormones in policemen].

Objective: To explore the relationship of occupational stressors with the serum levels of triiodothyronine (T3), thyroxine (T4), and thyroid stimulating hormone (TSH).

Methods: Using convenience sampling and cluster sampling methods, 225 policemen from a local police station in China were enrolled as subjects. Questionnaires were used to investigate demographic features and occupational stressors in those subjects.

[Effects of occupational stress and related factors to the mood of speed train drivers].

Objective: To explore the effect of occupational stress and related factors to the mood of speed train drivers.

Methods: By using cluster sampling method, a cross-sectional study was conducted in 1 352 speed train drivers (including 291 passenger train drivers, 640 freight trains drivers, 342 passenger shunting train drivers, and 79 High Speed Rail drivers) from a Railway Bureau depot. The survey included mood, individual factors, occupational stress factors, personality factors and mitigating factors.

[Effect of occupational stress and effort-reward imbalance on sleep quality of people's policeman].

Objective: To explore the effect of occupational stress and effort-reward imbalance on sleep quality of people's police.

Methods: A cluster sampling survey of sleep quality and occupational stress correlated factors was conducted on 287 police from a city public security bureau by questionnaires in May, 2011; the relationship between sleep quality and occupational stress correlated factors was analyzed by one-way ANOVA and multivariate non-conditional logistic regression using effort-reward imbalance model (ERI) and demand-control-support model (DCS). And the subjects were divided into high tension group and low tension group using the 1.

Association between psychosocial job characteristics and sickness absence due to low back symptoms using combined DCS and ERI models.

Objective: To evaluate the combined demand-control-support (DCS) and effort-reward-overcommitment (ERI-OC) stress models in association with sickness absence due to low back symptoms (SA-LBS).

Methods: A total of 2,737 blue-collar workers recruited from 13 companies in the most populous province (Henan) of China were included in the study. Personal and physical job characteristics, psychosocial scales of the stress models, and SA-LBS data in the preceding year were collected by a self-reported questionnaire and analyzed by a multivariable logistic regression model.