Roles and regulatory patterns of protein isoforms in plant adaptation and development.

Protein isoforms (PIs) play pivotal roles in regulating plant growth and development that confer adaptability to diverse environmental conditions. PIs are widely present in plants and generated through alternative splicing (AS), alternative polyadenylation (APA), alternative initiation (AI), and ribosomal frameshifting (RF) events. The widespread presence of PIs not only significantly increases the complexity of genomic information but also greatly enriches regulatory networks and enhances their flexibility.

MDV-encoded protein kinase U3 phosphorylates WTAP to inhibit transcriptomic mA modification and cellular protein translation.

Marek's disease virus (MDV)-encoded U3 is a highly conserved serine/threonine protein kinase in alpha-herpesviruses. In other alpha-herpesviruses, such as pseudorabies virus (PRV), U3 phosphorylates the N6-methyladenosine (mA) methyltransferase Wilms tumor 1-associated protein (WTAP), inhibiting mA modification. However, the role and mechanism of U3-mediated WTAP phosphorylation during MDV infection remain undefined.

Transcription factor Pofst3 regulates Pleurotus ostreatus development by targeting multiple biological pathways.

Pleurotus ostreatus is a popular edible mushroom cultivated worldwide. However, the mechanism of P. ostreatus primordia formation is unclear.

Black soybean peptide mediates the AMPK/SIRT1/NF-κB signaling pathway to alleviate Alzheimer's-related neuroinflammation in lead-exposed HT22 cells.

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by hyperphosphorylation of tau, neuroinflammation, and amyloid-beta (Aβ) plaques. Lead (Pb) exposure has been linked to an increased risk of AD and neuroinflammation. The purpose of this study is to determine if black soybean peptide (BSP1) may reduce neuroinflammation caused by Pb and associated AD-like pathology.

Distinct dynamic regulation of pectoralis muscle metabolomics by insulin and the promotion of glucose-lipid metabolism with extended duration.

Birds' glycolipid metabolism has garnered considerable attention due to their fasting blood glucose levels being nearly twice those of mammals. While skeletal muscle is the primary insulin-sensitive tissue in mammals, the effects of insulin on chicken skeletal muscle remain unclear. In this study, the insulin-responsive metabolites were identified in broiler's pectoralis muscle (after 16 h of fasting) using widely targeted metabolomics.

Variations in the milk lipidomic profile of lactating dairy cows fed the diets containing alfalfa hay versus alfalfa silage.

Alfalfa is primarily stored as silage or hay in livestock production. Previous research has shown that the storage method of grass significantly influences milk composition. This study aimed to investigate milk production performance and lipid composition in dairy cows fed diets consisting of alfalfa hay or alfalfa silage as roughage.

Effect of carrageenan on stability and 3D printing performance of high internal phase pickering emulsion stabilized by soy protein isolate aggregates under neutral condition.

High internal phase Pickering emulsion (HIPPE) stabilized by heat induced soy protein isolate aggregates (HSPI) alone had limited stability and poor 3D printing performance. While there is few research about HIPPE stabilized by HSPI and polysaccrides at neutral pH condition, where HSPI and ĸ-carrageenan (CG) were combined to fabricate HIPPE in this research. It was found that the incorporation of CG significantly decreased the droplet size and improved the storage stability of the resulting HIPPE.

Unraveling genetic mysteries: A comprehensive review of GWAS and DNA insights in animal and plant pathosystems.

DNA serves as the carrier of genetic information, with sequence variations playing a pivotal role in defining hereditary traits. Genome-Wide Association Studies (GWAS) facilitate the investigation of the links between genetic variations and phenotypes, significantly influencing biological research, particularly in animal and plant pathology. By identifying genetic markers associated with specific traits or diseases, GWAS enhances our understanding of host-pathogen interactions and improves disease-resistant breeding strategies.

Inhibiting UGCG prevents PRV infection by decreasing lysosome-associated autophage.

Glucosylceramide synthase (UGCG) is a key enzyme that catalyzes the initial glycosylation step in the biosynthesis of glycosphingolipids (GSLs) derived from glucosylceramide. UGCG is closely associated with various cellular processes, including the cell cycle, angiogenesis, multidrug resistance, and pathogen invasion. In this study, a short hairpin RNA (shRNA) library designed to target key genes involved in the sphingolipid metabolic pathway was utilized to elucidate their roles in Pseudorabies Virus (PRV).

[Characteristics, Sources, and Health Risk Changes of Heavy Metal Pollution Carried by PM in Beijing and Evaluation of Policy Effectiveness].

To evaluate the effectiveness of air pollution prevention and control measures in Beijing, this study measured the content of 13 metal elements, including seven heavy metal elements [As, Cd, Co, Cr（Ⅵ）, Ni, Pb, and V], through daily PM sampling （ = 934） in the urban area of Beijing for four years. We analyzed the interannual changes in the concentration levels of various metals and the differences between heating and non-heating seasons, used a positive definite matrix factorization （PMF） model for quantitative source analysis, and used health risk assessment methods to evaluate the health risks of six metals. The results showed that, except for a few metal elements such as Cr（Ⅵ） and Ni, which showed an increase in concentration in individual years, the overall concentration of each element showed a downward trend.

The effects of procrastination on physical activity among Chinese university students: the chain-mediated effects of time management disposition and exercise motivation.

Objectives: Grounded in self-determination theory (SDT), the procrastination-health model, and the mechanism model of exercise persistence, this study examined the effects of procrastination on physical activity and the mechanism of its action in Chinese college students.

Design: This study employed a cross-sectional design.

Methods: A total of 957 Chinese university students (Mage = 20.

Analysis of NRAMP genes in the Triticeae reveals that TaNRAMP5 positively regulates cadmium (Cd) tolerance in wheat (Triticum aestivum).

Natural Resistance-Associated Macrophage Protein (NRAMP), a class of metal transporter proteins widely distributed in plants, is mainly involved in the uptake and transport by plants of metal ions, such as iron, manganese and cadmium. The current study is the first to fully investigate the Triticum aestivum (T. aestivum) NRAMP gene family.

Insights into the effects of frozen storage on the rheology, texture and in vitro digestibility of frozen Liangpi (starch gel food).

In this study, the effects of frozen storage on the rheology, texture and in vitro digestibility of frozen Liangpi and its internal influencing mechanism were investigated. The results showed that with increasing frozen storage time, the solid-like property proportion of frozen Liangpi gradually enhanced, and its hardness and chewiness gradually increased, and its springiness gradually decreased, while its digestion rate gradually slowed down. During frozen storage, the water fluidity of frozen Liangpi gradually enhanced, and its starch molecules rearrangement degree gradually deepened.

End-point RPA-CRISPR/Cas12a-based detection of Enterocytozoon bieneusi nucleic acid: rapid, sensitive and specific.

Enterocytozoon bieneusi is a common species of microsporidia that infects humans and animals. Current methods for detecting E. bieneusi infections have trade-offs in sensitivity, specificity, simplicity, cost and speed and are thus unacceptable for clinical application.

Deciphering antibiotic resistance genes and plasmids in pathogenic bacteria from 166 hospital effluents in Shanghai, China.

Although previous studies using phenotypic or metagenomic approaches have revealed the patterns of antibiotic resistance genes (ARGs) in hospital effluents in local regions, limited information is available regarding the antibiotic resistome and plasmidome in human pathogenic bacteria in hospital effluents of megacity in China. To address this knowledge gap, we analyzed effluent samples from 166 hospitals across 13 geographical districts in Shanghai, China, using both cultivation-based approaches and metagenomics. A total of 357 strains were isolated from these samples, with the predominant species being Escherichia coli (n = 61), Aeromonas hydrophila (n = 57), Klebsiella pneumoniae (n = 48), and Aeromonas caviae (n = 42).

LsKN1 and LsOFP6 synergistically regulate the bolting time by modulating the gibberellin pathway in lettuce.

Bolting time is an important agronomic trait in lettuce (Lactuca sativa) production. Premature bolting significantly reduces crop quality and marketability. Here, we report map-based cloning and characterization of a LsKN1 gene that controls bolting in lettuce.

Data-driven models for forecasting algal biomass in a large and deep reservoir.

Early warning of algal biomass is important for the preservation and management of drinking water. However, accurate prediction of algal biomass in large and deep reservoirs remains a challenge. Here, we used six years of high-frequency observations (30 min/time) to train long short-term memory (LSTM) models for forecasting chlorophyll-a concentration (C) and column-integrated C (CIC) for a large and deep Chinese reservoir (Xin'anjiang Reservoir).

Mechanism of a novel JNF2 in suppressing f. sp. and enhancing cucumber growth.

Cucumber wilt caused by f. sp. (), is a prevalent soil-borne disease.