Coupled Electrochemical and Microbial Catalysis for the Production of Polymer Bricks.

Power-to-X technologies have the potential to pave the way towards a future resource-secure bioeconomy as they enable the exploitation of renewable resources and CO . Herein, the coupled electrocatalytic and microbial catalysis of the C -polymer precursors mesaconate and 2S-methylsuccinate from CO and electric energy by in situ coupling electrochemical and microbial catalysis at 1 L-scale was developed. In the first phase, 6.

Label-Free Four-Dimensional Visualization of Anaerobically Growing Electroactive Biofilms.

Light sheet fluorescence microscopy (LSFM) allows nondestructive, label-free and in vivo imaging of large specimen, even at nontransparent surfaces. We show that LSFM can be applied for label-free analyses of prokaryotes on the example of electroactive biofilms. Biofilm growth is linked to the production of current serving as measure of metabolic activity in vivo by monitoring with high spatial and temporal resolution.

Adipose tissue derived bacteria are associated with inflammation in obesity and type 2 diabetes.

Objective: Bacterial translocation to various organs including human adipose tissue (AT) due to increased intestinal permeability remains poorly understood. We hypothesised that: (1) bacterial presence is highly tissue specific and (2) related in composition and quantity to immune inflammatory and metabolic burden.

Design: We quantified and sequenced the bacterial 16S rRNA gene in blood and AT samples (omental, mesenteric and subcutaneous) of 75 subjects with obesity with or without type 2 diabetes (T2D) and used catalysed reporter deposition (CARD) - fluorescence in situ hybridisation (FISH) to detect bacteria in AT.

Electron balancing under different sink conditions reveals positive effects on photon efficiency and metabolic activity of sp. PCC 6803.

Background: Cyanobacteria are ideal model organisms to exploit photosynthetically derived electrons or fixed carbon for the biotechnological synthesis of high value compounds and energy carriers. Much effort is spent on the rational design of heterologous pathways to produce value-added chemicals. Much less focus is drawn on the basic physiological responses and potentials of phototrophs to deal with natural or artificial electron and carbon sinks.

Equilibrium sampling reveals increasing thermodynamic potential of polycyclic aromatic hydrocarbons during sewage sludge digestion.

The reuse of digested sludge from wastewater treatment plants (WWTPs) as soil fertilizer poses a risk for contamination of soil and water environments. The present study provides a new approach for investigating the exposure of hydrophobic organic chemicals in sewage sludge. The methodology of equilibrium sampling with multiple thicknesses of silicone was successfully validated and applied to complex sludge matrices.

Lipidomics of the sea sponge Amphimedon queenslandica and implication for biomarker geochemistry.

Demosponges are a rich natural source of unusual lipids, some of which are of interest as geochemical biomarkers. Although demosponges are animals, they often host dense communities of microbial symbionts, and it is therefore unclear which lipids can be synthesized by the animal de novo, and which require input from the microbial community. To address this uncertainty, we analyzed the lipids of Amphimdeon queenslandica, the only demosponge with a published genome.

Predicting and experimental evaluating bio-electrochemical synthesis - A case study with Clostridium kluyveri.

Microbial electrosynthesis is a highly promising application of microbial electrochemical technologies for the sustainable production of organic compounds. At the same time a multitude of questions need to be answered and challenges to be met. Central for its further development is using appropriate electroactive microorganisms and their efficient extracellular electron transfer (EET) as well as wiring of the metabolism to EET.