Karst Collapse Monitoring and Early Warning Evaluation Method Based on Multisensor Internet of Things.

The international community has paid extensive attention to the numerous engineering problems faced by karst areas caused by the increasingly frequent human activities. China has a wide variety of karst forms. Among them, carbonate karst is the most widely distributed, and the development of carbonate karst is relatively strong in many areas.

Optimization of College English Classroom Teaching Efficiency by Deep Learning SDD Algorithm.

In order to improve the teaching efficiency of English teachers in classroom teaching, the target detection algorithm in deep learning and the monitoring information from teachers are used, the target detection algorithm of deep learning Single Shot MultiBox Detector (SSD) is optimized, and the optimized Mobilenet-Single Shot MultiBox Detector (Mobilenet-SSD) is designed. After analyzing the Mobilenet-SSD algorithm, it is recognized that the algorithm has the shortcomings of large amount of basic network parameters and poor small target detection. The deficiencies are optimized in the following partThrough related experiments of student behaviour analysis, the average detection accuracy of the optimized algorithm reached 82.

ROS-Activated homodimeric podophyllotoxin nanomedicine with self-accelerating drug release for efficient cancer eradication.

Although podophyllotoxin (POD) demonstrates high efficiency to inhibit various cancers, its clinic application is limited to poor bioavailability. Nanoparticles derived from homodimeric prodrugs with high drug loading potential are emerging as promising nanomedicines. However, complete intracellular drug release remains a major hindrance to the use of homodimeric prodrugs-based nanomedicine.

Characterization of the metabolites of dichotomitin in rat, monkey and human by ultra-high-performance liquid chromatography/high-resolution mass spectrometry.

Rationale: Dichotomitin is one of the bioactive constituents isolated from Belamcanda chinensis. The goal of this study was to identify the metabolites of dichotomitin produced by liver microsomes and hepatocytes.

Methods: Using ultra-high-performance liquid chromatography and high-resolution mass spectrometry (UPLC/HRMS), the metabolites were profiled and identified.

Tanshinone IIA induces ferroptosis in gastric cancer cells through p53-mediated SLC7A11 down-regulation.

Gastric cancer represents a malignant type of cancer worldwide. Tanshinone IIA (Tan IIA), a pharmacologically active component isolated from the rhizome of the Chinese herb Salvia miltiorrhiza Bunge (Danshen), has been reported to possess an anti-cancer effect in gastric cancer. However, its mechanisms are still not fully understood.

A novel LC-MS/MS method for the determination of ziritaxestat in rat plasma and its pharmacokinetic study.

Ziritaxestat is a first-in-class autotoxin inhibitor. The purpose of this study was to develop a liquid chromatography/electrospray ionization tandem mass spectrometric (LC-MS/MS) method for the determination of ziritaxestat in rat plasma. The plasma sample was deproteinated using acetonitrile and then separated on an Acquity BEH C column with water containing 0.

Transformation of tobacco plants by Yali PPO-GFP fusion gene and observation of subcellular localization.

To explore the subcellular localization of Polyphenol oxidase (PPO) from Pyrus bretschneideri, the 1779 bp cDNA of PPO gene excluding the termination codon TAA was cloned and fused with GFP to construct a binary vector pBI121-PPO-GFP. Then, the binary vector was transformed into Nicotiana tabacum by the tumefanciens-mediated method. Using confocal laser scanning microscopy, green fluorescent signals were localized in chloroplasts of the transformed Nicotiana tabacum cell, suggesting that the Polyphenol oxidase from Pyrus bretschneideri was a chloroplast protein.

Complementary DNA cloning of the pear 1-aminocyclopropane-1-carboxylic acid oxidase gene and agrobacterium-mediated anti-sense genetic transformation.

The aim of the present study was to genetically modify plantlets of the Chinese yali pear to reduce their expression of ripening-associated 1-aminocyclopropane-1-carboxylic acid oxidase (ACO) and therefore increase the shelf-life of the fruit. Primers were designed with selectivity for the conserved regions of published ACO gene sequences, and yali complementary DNA (cDNA) cloning was performed by reverse transcription quantitative polymerase chain reaction (PCR). The obtained cDNA fragment contained 831 base pairs, encoding 276 amino acid residues, and shared no less than 94% nucleotide sequence identity with other published ACO genes.