A new method for detecting berberine in Rhizoma coptidis using β-cyclodextrin-sensitized fluorescence technology has been developed, allowing for sensitive measurement of this medicinal compound.
The method utilizes specific wavelengths (excitation at 345 nm and emission at 540 nm) to avoid interference and demonstrate a low limit of detection alongside a broad linear range of concentrations.
Testing revealed that berberine constitutes 7.60% of the Rhizoma coptidis sample, with a recovery rate of 99.5%, and results were consistent with another established detection method, indicating its reliability and practicality for medicinal applications.