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Article Synopsis
  • A new method for detecting berberine in Rhizoma coptidis using β-cyclodextrin-sensitized fluorescence technology has been developed, allowing for sensitive measurement of this medicinal compound.
  • The method utilizes specific wavelengths (excitation at 345 nm and emission at 540 nm) to avoid interference and demonstrate a low limit of detection alongside a broad linear range of concentrations.
  • Testing revealed that berberine constitutes 7.60% of the Rhizoma coptidis sample, with a recovery rate of 99.5%, and results were consistent with another established detection method, indicating its reliability and practicality for medicinal applications.
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