Isolation and characterization of CD105+/CD90+ subpopulation in breast cancer MDA-MB-231 cell line.

Background: The epithelial-mesenchymal transition (EMT) generates cells with properties of stem cells, if that happened, the stem cell should be with mesenchymal property. This study aimed to identify a group of cells with mesenchymal stem cell (MSC)-like characteristics in breast cancer bone metastatic cell line MDA-MB-231, moreover, the relevance between breast cancer stem cells and the EMT was observed. CD105 and CD90, identified as the standards of MSCs, were used for the identification.

Dryocrassin ABBA, a novel active substance for use against amantadine-resistant H5N1 avian influenza virus.

The occurrence of multi-drug resistant highly pathogenic avian influenza virus (HPAIV) strains highlights the urgent need for strategies for the prevention and control of avian influenza virus. The aim of our current study is to evaluate the antiviral activity of dryocrassin ABBA isolated from Rhizoma Dryopteridis Crassirhizomatis (RDC) against an amantadine-resistant H5N1 (A/Chicken/Hebei/706/2005) strain in a mouse model. Post inoculation with HPAIV H5N1 virus in mice, the survival rate was 87, 80, and 60% respectively in the 33, 18, and 12.

Short versus long intramedullary nails for the treatment of intertrochanteric hip fractures in patients older than 65 years.

Objective: The purpose of this study was to compare failure rates between short and long intramedullary nails used for the treatment of intertrochanteric hip fractures in patients over 65 years of age.

Methods: A retrospective review of 156 patients aged more than 65 years with femoral intertrochanteric fractures from December 2010 to December 2012 was performed. The patients were allocated to two groups: those treated with long nail (n=59) and short nail (n=97).

Ulnar impaction syndrome with different operative methods: a comparative biomechanical study.

Objective: Ulnar impaction syndrome seriously impairs wrist and hand function. Three main treatment procedures are available; however, little systematic research on the post-operation changes in wrist biomechanics currently exists. This study aimed to determine the long-term effects of these procedures and the optimal treatment methods for ulnar impaction syndrome.

Bone fracture and the interaction between bisphosphonates and proton pump inhibitors: a meta-analysis.

Objective: Recent studies suggested an increased risk of fractures with interaction between bisphosphonates (BPs) and proton pump inhibitors (PPIs). We performed a meta-analysis of fractures between patients taking BPs/PPIs and those taking BPs only.

Methods: We conducted a PubMed database and Ovid database search, as well as Cochrane Library search (up to July 2014) for studies assessing the association between fractures and BPs or/and PPIs.

Chinese medicine Jinlida (JLD) ameliorates high-fat-diet induced insulin resistance in rats by reducing lipid accumulation in skeletal muscle.

The present paper reports the effects of Jinlida (JLD), a traditional Chinese medicine which has been given as a treatment for high-fat-diet (HFD)-induced insulin resistance. A randomized controlled experiment was conducted to provide evidence in support of the affects of JLD on insulin resistance induced by HFD. The affect of JLD on blood glucose, lipid, insulin, adiponectin, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and total bilirubin (TBIL) in serum and lipid content in skeletal muscle was measured.

Relationship between thioredoxin-interacting protein (TXNIP) and islet β-cell dysfunction in patients with impaired glucose tolerance and hypertriglyceridemia.

Aims: To study the relationship between thioredoxin-interacting protein (TXNIP) and pancreatic β-cell function in patients with impaired glucose regulation and patients with both impaired glucose regulation and hypertriglyceridemia.

Methods: We analyzed a population of 90 patients with impaired glucose regulation (IGR), 87 patients with IGR and hypertriglyceridemia, and 90 subjects with normal glucose tolerance (NGT). The levels of plasma TXNIP, a regulator of cellular oxidative stress, were measured.

Analysis of correlative risk factors for C5 palsy after anterior cervical decompression and fusion.

Background: It has been reported that C5 palsy is a potential complication of both anterior and posterior cervical spine surgery, although several mechanisms of C5 palsy following posterior cervical surgery have been proposed, few reports about correlative risk factors have been elaborated on C5 palsy after anterior cervical decompression and fusion (ACDF).

Objective: The purpose of this study was to investigate the correlative risk factors of C5 palsy after anterior cervical decompression and fusion.

Methods: This is a retrospective study.

Clinical efficacy and prognosis factors of open calcaneal fracture: a retrospective study.

Background: Treatment of open calcaneal fractures remains to be a challenge for orthopaedic surgeons. The aim of this study is to assess factors affecting the treatment results of open calcaneal fractures.

Methods: A total of 98 patients who have 101 open calcaneal fractures were recruited in our hospital, they were all treated with a standard protocol based on the appearance of the traumatic wound.

Unilateral posterior vertebral column resection for severe thoracolumbar kyphotic deformity caused by old compressive vertebrae fracture: a technical improvement.

Severe thoracolumbar kyphotic deformity caused by old compressive vertebrae fracture remains a big challenge for spine surgeons. When symptoms related to significant deformities cannot be adequately managed conservatively, posterior vertebral column resection (PVCR) is required, but with long operating time and severe blood loss. We develop a UPVCR technique, which is done through a unilateral approach instead of a bilateral approach, vertebral body resection advancing to cross the midline in an abrasive way from an extreme oblique orientation enable the resection of most contralateral vertebral body.

SHIP2 on pI3K/Akt pathway in palmitic acid stimulated islet β cell.

This study is to investigate the influence of SHIP2 on palmitic acid stimulated islet β cell and insulin secretion, as well as its role in pI3K/Akt pathway. We defined four groups: control, acid group, acid + NC siRNA group and acid + siRNA transfection group. The control was neither treated by palmitic acid nor transfection.

Prediction of cerebrospinal fluid parameters for tuberculous meningitis.

Background: Tuberculous meningitis is the most lethal form of tuberculosis, but current diagnostic methods are inadequate. The measurement of cerebrospinal fluid parameters can provide early information for diagnosis. The present study focus on the validity of the cut-off value of cerebrospinal fluid parameters according to the Lancet consensus of scoring system for diagnosis of tuberculous meningitis.

Modulation of the function of dendritic cells in adolescents with chronic HBV infection by IFN-λ1.

The exact immunology pathogenesis of hepatitis B virus (HBV) infection remains unclear currently. The dendritic cells (DCs) dysfunction is evident in adolescents with chronic HBV infection in the immune tolerant phase. DCs, as the most efficient professional antigen-presenting cells (APCs), possess the strongest antigen presenting the effect in the body and can stimulate the initial T cell activation and proliferation, depending on their stage of maturation.

Correlative analyses of isolated upper lumbar disc herniation and adjacent wedge-shaped vertebrae.

Background: Upper lumbar disc herniation (ULDH) is easy to be misdiagnosed due to its special anatomical and atypical clinical features. Few studies have identified the relationship between ULDH and adjacent wedge-shaped vertebrae (WSV).

Hypothesis: WSV may have some indicative relations withULDH.

PIAS3, an inhibitor of STAT3, has intensively negative association with the survival of gastric cancer.

Objective: The present study was conducted to elucidate the prognostic prediction value of the expression of the protein inhibitor of activated signal transducer and activators of transcription 3 (PIAS3) in gastric cancer (GC).

Methods: We detected the expression of PIAS3 in GC tissue, adjacent non-tumor tissue, GC cell lines, and GES-1 cell line. Besides, both clinicopathological data and follow-up records were obtained for patients' survival analyses.

Study of functional connectivity in patients with sensorineural hearing loss by using resting-state fMRI.

Objective: To determine functional connectivity of the default mode network in patients with sensorineural hearing loss (SNHL) in resting state.

Methods: The posterior cingulate cortex was selected as a seed for assessment of functional connectivity of the activated brain areas in resting state by using a seed-based correlation analysis of the resting state functional magnetic resonance imaging (fMRI) data.

Results: The fMRI results demonstrated that, the healthy volunteers and the patients with NSHL shared certain activated brain areas with positive functional connectivity with region of interest (ROI).

The efficacy and safety of adding bevacizumab to cetuximab- or panitumumab-based therapy in the treatment of patients with metastatic colorectal cancer (mCRC): a meta-analysis from randomized control trials.

Objective: To estimate the efficacy and safety of adding bevacizumab to cetuximab- or panitumumab-based therapy in the treatment of patients with metastatic colorectal cancer (mCRC), using a meta-analysis of randomized controlled trials.

Methods: A literature search for randomized clinical trials (RCTs) was performed through Pubmed, Embase, and Web of Science (up to May 22, 2014). The outcome measures were progression-free survival (PFS), overall survival (OS), objective response rate (ORR), and adverse events.

Familial clustering of overweight and obesity among schoolchildren in northern China.

Background: We aimed to study the prevalence of overweight and obesity and to assess its familial clustering among schoolchildren in northern China.

Methods: A cross-sectional study was conducted on 95,292 schoolchildren in northern China to investigate the prevalence of overweight and obesity. A group of overweight and obese children (n = 450) was selected using a cluster sampling method.

Effects of low-dose spironolactone combined with irbesartan on cardiac hypertrophy induced by pressure overload in rats.

Background: The mineralocorticoid receptor (MR) blockade in the heart is an attractive therapeutic option for the treatment of heart failure. However, the use of MR antagonist is limited by an increased incidence of hyperkalemia owing to MR blockade in the kidney. This study was designed to evaluate and compare the effectiveness of a low, non-pressure-lowering dose of spironolactone (Sp) with that of a conventional blood pressure-lowering dose combined with irbesartan on pathological cardiac remodeling as well as serum potassium level in pressure-overload rats.

A key mediator, PTX3, of IKK/IκB/NF-κB exacerbates human umbilical vein endothelial cell injury and dysfunction.

Objective: This study was performed to investigate PTX3-mediated iNOS expression and IKK/IκB/NF-κB activation in PA-induced atherosclerotic HUVECs injury model.

Methods: The cell viability was detected by the CCK8 assay. The cell apoptosis was assessed by annexin V-PI double-labeling staining.

Regulation of apoptosis by long non-coding RNA HIF1A-AS1 in VSMCs: implications for TAA pathogenesis.

Objective: Long non-coding RNAs (lncRNAs) play important roles in diverse biological processes, such as transcriptional regulation, cell growth and tumorigenesis. However, little was known about whether lncRNA HIF 1 alpha-antisense RNA 1 (HIF1a-AS1) in regulating the proliferation and apoptosis of VSMCs in vitro and the expression of HIF1a-AS1 in serum of TAA patients.

Methods: The cell viability was detected by the CCK8 assay.

Inhibition of Vav3 could reverse the drug resistance of gastric cancer cells by downregulating JNK signaling pathway.

This study aims to investigate the effect and mechanism of Vav3 on the multidrug resistance of gastric cancer. Fluorescence quantitative RT-PCR and western blot assay were used to detect Vav3 and drug resistance genes in gastric cancer tissues as well as gastric cell lines such as SGC7901, SGC7901/adriamycin (ADR) and GES-1. Besides, Vav3-specific small interfering RNA (Vav3-siRNA) was applied to inhibit Vav3 in SGC7901/ADR, and SRB assay was used to determine chemosensitivity.

Effect of Huazhuojiedu medicated serum on the proliferation and activation of hepatic stellate cells and the expression of PI3K and p-Akt in rats.

To observe the effect of Huazhuojiedu medicated serum on the proliferation and activation of hepatic stellate cells, as well as the expression of PI3K and p-Akt in rats, and to explore the underlying mechanism of Huazhuojiedu prescription against hepatic fibrosis. Hepatic stellate cells harvested from rats were resuscitated and subcultured, followed by the intervention of Huazhuojiedu equivalent dose, Huazhuojiedu double dose, and positive drug (Compound Biejiaruangan Troche) medicated serum of rats. After in vitro culture, hepatic stellate cells were stimulated with 5 ng/mL transforming growth factor-β1.

Expression of annexin A7 and its clinical significance in differentiation and metastasis of gastric carcinoma.

Objective: To investigate the expression and clinical significance of annexin A7 in the differentiation and lymphatic metastasis of gastric cancer (GC).

Methods: The clinical and pathological data were recorded for analysis. Immunohistochemical staining and Western blot were performed to analyze the expression of ANXA 7 in primary GC tissues.