258 results match your criteria: "Heavy Chain Disease Mu"
[High expression of variable domain of heavy-chain antibodies in Expi293F cells with optimized signal peptide and codons].
Sheng Wu Gong Cheng Xue Bao
November 2024
State Key Laboratory for Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730000, Gansu, China.
Article Synopsis
- The study focuses on improving the production of heavy-chain antibodies (VHH) for drug therapy and research by optimizing expression systems.
- The researchers identified an effective signal peptide (SP IFN-α2) that enhanced VHH secretion and implemented codon optimization to significantly increase yield while maintaining functional binding activity.
- The optimized process resulted in an average yield of over 191.6 mg/L for multiple VHHs, indicating a promising method for large-scale production in mammalian cells, particularly Expi293F cells.
B-cells absence in patients diagnosed as inborn errors of immunity: a registry-based study.
Immunogenetics
June 2024
Immunodeficiency Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran.
Article Synopsis
- - Hypogammaglobulinemia without B-cells is a type of inborn error of immunity marked by low serum immunoglobulins and a lack of B-cells, with most cases linked to mutations in the BTK gene.
- - In a study of 27 patients from 13 families, researchers discovered several novel mutations in the BTK gene along with other genetic variants in different genes, which contribute to the condition's complexity.
- - The findings expand the understanding of this immunological disorder, especially within the Iranian community, highlighting that affected siblings may exhibit better disease management compared to other family members.
affinity maturation of the HIV-1 Env-binding domain of CD4.
Res Sq
February 2024
Skaggs Graduate School, Scripps Research, La Jolla, CA 92037, USA.
Article Synopsis
- - The study focuses on engineering a human protein biologic, specifically a half-life enhanced HIV-1 entry inhibitor, into murine B cells without compromising their natural ability to improve binding affinity over time.
- - By introducing CD4 domains into the heavy-chain loci of these B cells and transferring them into wild-type mice, the modified cells successfully proliferated and produced antibodies that neutralize HIV-1 more effectively.
- - The findings suggest that affinity maturation techniques can enhance the therapeutic potential of non-antibody protein biologics, improving their effectiveness without losing important pharmacokinetic qualities.
affinity maturation of the HIV-1 Env-binding domain of CD4.
bioRxiv
February 2024
Skaggs Graduate School, Scripps Research, La Jolla, CA 92037, USA.
Article Synopsis
- Researchers are engineering human proteins for clinical use but face challenges like sensitivity to proteases and self-reactivity, which can limit effectiveness.
- The study specifically enhances B-cell receptors in mice to express a modified HIV-1 entry inhibitor, allowing B cells to mature and produce antibodies that can bind more effectively to HIV-1.
- This approach resulted in a more than ten-fold increase in the ability of the modified protein to neutralize various HIV-1 strains, paving the way for better therapeutic development without losing desirable drug properties.
Meibomian Gland Dysfunction Is Associated with Low Levels of Immunoglobulin Chains and Cystatin-SN.
Int J Mol Sci
October 2023
Department of Clinical Medicine, Aalborg University, 9000 Aalborg, Denmark.
Article Synopsis
- Meibomian gland dysfunction (MGD) is a common condition that leads to evaporative dry eye disease, and studying its proteome can improve treatment strategies.
- Researchers collected and analyzed tear samples from patients with MGD and healthy controls, discovering 88 differentially expressed proteins.
- Notably, cystatin-SN was found to be downregulated in MGD, correlating with decreases in tear meniscus height, while various immunoglobulin components related to inflammation and immunity were also downregulated, suggesting inflammation from tear evaporation impacts these proteins.
The Endolysosomal Transporter DMT1 is Required for Morphine Regulation of Neuronal Ferritin Heavy Chain.
J Neuroimmune Pharmacol
September 2023
Department of Pharmacology & Physiology, Drexel University College of Medicine, 245 North 15th Street, Philadelphia, PA, 19102, USA.
Article Synopsis
- NeuroHIV and other neurological disorders disrupt iron metabolism in neurons, and opioid use, like morphine, can exacerbate these issues by altering this metabolism further.
- The study focused on the role of divalent metal transporter 1 (DMT1) in the process where morphine causes neurons to release iron from endolysosomes and upregulate ferritin heavy chain (FHC), a protein linked to cognitive decline in NeuroHIV.
- Blocking DMT1 using a pharmacological inhibitor or silencing it genetically prevented morphine from increasing FHC levels, revealing that DMT1 is essential for morphine’s effects on neuronal iron regulation and cognitive function.
Site of vulnerability on SARS-CoV-2 spike induces broadly protective antibody against antigenically distinct Omicron subvariants.
J Clin Invest
April 2023
Drukier Institute for Children's Health, Department of Pediatrics, Weill Cornell Medicine, New York, New York, USA.
The rapid evolution of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variants has emphasized the need to identify antibodies with broad neutralizing capabilities to inform future monoclonal therapies and vaccination strategies. Herein, we identified S728-1157, a broadly neutralizing antibody (bnAb) targeting the receptor-binding site (RBS) that was derived from an individual previously infected with WT SARS-CoV-2 prior to the spread of variants of concern (VOCs). S728-1157 demonstrated broad cross-neutralization of all dominant variants, including D614G, Beta, Delta, Kappa, Mu, and Omicron (BA.
View Article and Find Full Text PDFCorrection: Mu heavy chain disease with MYD88 L265P mutation: An unusual manifestation of lymphoplasmacytic lymphoma.
Diagn Pathol
October 2022
Department of Pathology, University of Pittsburgh and UPMC, Pittsburgh, PA, USA.
Reduction of senescent fibro-adipogenic progenitors in progeria-aged muscle by senolytics rescues the function of muscle stem cells.
J Cachexia Sarcopenia Muscle
December 2022
School of Pharmacy and Pharmaceutical Science, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, China.
Article Synopsis
- Fibro-adipogenic progenitors (FAPs) in muscle tissues help muscle regeneration but become senescent in aging, affecting their interaction with muscle progenitor/stem cells (MPCs), especially in Hutchinson-Gilford progeria syndrome (HGPS).
- In a study comparing normal WT mice to Zmpste24 (Z24) mice, Z24 mice showed significantly more FAPs and fewer MPCs, with a high percentage of senescent FAPs, which negatively impacted the proliferation and muscle formation capabilities of MPCs.
- Administering the senolytic drug fisetin successfully reduced senescent FAPs, improved the function of MPCs, and showed promising results in
Mu heavy chain disease with MYD88 L265P mutation: an unusual manifestation of lymphoplasmacytic lymphoma.
Diagn Pathol
August 2022
Department of Pathology, University of Pittsburgh and UPMC, Pittsburgh, PA, USA.
Article Synopsis
- Mu heavy chain disease is a rare condition involving abnormal plasma cells and defective mu immunoglobulin heavy chains, and its biological mechanisms are not well understood.
- A specific case was reported involving a patient with a MYD88 L265P mutation and deletion of 6q, both linked to lymphoplasmacytic lymphoma/Waldenström macroglobulinemia, identified through serum mass spectrometry.
- The study found that lymphocytosis is rare in mu heavy chain disease and suggests that some cases resemble lymphoplasmacytic lymphoma more than chronic lymphocytic leukemia, especially due to the common MYD88 mutation in these patients.
CD137L and CD4 T cells limit BCL6-expressing pre-germinal center B cell expansion and BCL6-driven B cell malignancy.
Immunol Cell Biol
October 2022
Department of Immunology and Pathology, Alfred Medical Research and Education Precinct, Monash University, Melbourne, VIC, Australia.
Article Synopsis
- * CD4 T cells play a significant role in controlling lymphomas in these mice, although the mechanisms behind this immune surveillance are not well understood.
- * The signaling from CD137 ligand (CD137L) is crucial for CD4 T cell action, as it helps transition pre-cancerous B cells into a less dangerous state, adding another protective layer against B cell malignancies.
Combination of KIT and FLT3-ITD mutation status with minimal residual disease levels guides treatment strategy for adult patients with inv(16) acute myeloid leukemia in first complete remission.
Hematol Oncol
October 2022
Peking University People's Hospital, Peking University Institute of Hematology, National Clinical Research Center for Hematologic Disease, Beijing Key Laboratory of Hematopoietic Stem Cell Transplantation, Beijing, China.
Article Synopsis
- A study analyzed the impact of allogeneic hematopoietic stem cell transplantation (allo-HSCT) on adult patients with inv (16) acute myeloid leukemia (AML), emphasizing the roles of KIT and FLT3-ITD mutations and minimal residual disease (MRD) levels in predicting outcomes.
- Among 157 patients, those with KIT mutations had a significantly higher risk of relapse compared to those without, while FLT3-ITD mutations also suggested an elevated risk.
- The research categorized patients into high-risk, low-risk, and intermediate-risk groups based on mutation and MRD status, finding that allo-HSCT notably improved outcomes, particularly for the high-risk group, while it was less effective
Association of IGHM polymorphisms with susceptibility to type 1 diabetes.
Immunol Res
June 2022
Immunology Department, Habib Bourguiba Hospital, University of Sfax, Sfax, Tunisia.
Article Synopsis
- The study investigates how specific genetic variants in the immunoglobulin heavy-chain (IGH) locus may influence the susceptibility to Type 1 Diabetes (T1D).
- It involved genotyping 255 individuals from 59 Tunisian families for 15 single nucleotide polymorphisms (SNPs) located in the IGH locus.
- Significant associations were found between certain SNPs and increased risk of T1D, suggesting that these genetic variations could contribute to the production of autoantibodies linked to the disease.
An oligomeric semiconducting nanozyme with ultrafast electron transfers alleviates acute brain injury.
Sci Adv
November 2021
Tianjin Key Laboratory of Brain Science and Neural Engineering, Academy of Medical Engineering and Translational Medicine, Tianjin University, Tianjin 300072, China.
Article Synopsis
- Artificial enzymes are gaining attention in medicine and biotechnology due to their stability, simple production, and cost efficiency, but their effectiveness is limited by slow electron transfer rates.
- The new oligomeric nanozyme (O-NZ) achieves incredibly fast electron transfer, resulting in significantly enhanced catalytic activity that matches natural enzymes in performance.
- O-NZ has shown promising results in improving survival rates and cognitive recovery in mice after acute brain trauma by reducing harmful compounds and inflammation in the body.
Screening differentially expressed genes between endometriosis and ovarian cancer to find new biomarkers for endometriosis.
Ann Med
December 2021
Department of Gynaecology and Obstetrics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Article Synopsis
- Endometriosis is a common reproductive system disease with unclear progression mechanisms and high recurrence rates, necessitating research on potential therapeutic biomarkers.
- The study analyzed datasets from endometriosis and ovarian cancer to identify differentially expressed genes (DEGs) and their roles in disease progression through various bioinformatics tools.
- A total of 104 DEGs were identified, with some linked to cell functions and drug potential, suggesting that certain genes could serve as therapeutic targets for managing endometriosis and its recurrence.
Light chain proteinuria revealing mu-heavy chain disease: an atypical presentation of Waldenström macroglobulinemia in two cases.
Haematologica
July 2021
Immuno-hematology Department, Saint-Louis Hospital, APHP, Paris.
A novel immune prognostic index for stratification of high-risk patients with early breast cancer.
Sci Rep
January 2021
Interdisciplinary Program in Bioinformatics, College of Natural Sciences, Seoul National University, Seoul, 41566, Republic of Korea.
Article Synopsis
- - The current multigene assays for breast cancer mainly work for a specific type of cancer, and they don't include important immune-related genes that could provide significant prognostic information.
- - Researchers classified breast cancer patients into three risk groups and created an immune prognostic index using five key immune genes, which helps predict the likelihood of cancer recurrence in high-risk patients.
- - This new immune prognostic index was found to provide stronger predictions for disease-free survival compared to traditional methods, showing potential to identify low-risk patients better than existing multigene tests.
A BCWD-Resistant line of rainbow trout is less sensitive to cortisol implant-induced changes in IgM response as compared to a susceptible (control) line.
Dev Comp Immunol
March 2021
Department of Biology, William and Mary, Williamsburg, VA, 23185, USA. Electronic address:
Article Synopsis
- Stress increases cortisol levels in salmonids, making them more susceptible to diseases like BCWD caused by Flavobacterium psychrophilum.
- In a study comparing a BCWD-resistant rainbow trout line (R-line) and a susceptible line (S-line), it was found that cortisol delayed the IgM immune response in both lines, but the resistance was linked to a quicker initial IgM response in the R-line.
- The results suggest that under stress, BCWD-resistant trout are better able to manage infections due to a more effective and less severe immune response compared to susceptible trout.
Opioid Modulation of Neuronal Iron and Potential Contributions to NeuroHIV.
Methods Mol Biol
March 2021
Department of Pharmacology & Physiology, Drexel University College of Medicine, Philadelphia, PA, USA.
Article Synopsis
- * Research has found that morphine raises iron levels in neurons and impacts the structure of dendritic spines, which are vital for learning and memory.
- * Understanding how opioids affect neuronal iron metabolism could lead to new drug targets for treating HIV-associated neurocognitive disorders and related diseases like Alzheimer's and Parkinson's.
Natural products as LSD1 inhibitors for cancer therapy.
Acta Pharm Sin B
June 2020
School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China.
Article Synopsis
- * Natural products have shown potential in targeting LSD1, an important enzyme linked to cancer, with various compounds like alkaloids and flavones acting as effective inhibitors.
- * The review focuses on identifying natural LSD1 inhibitors, examining their structures, antitumor effects, and mechanisms, while also addressing challenges in this research area.
Mouse Models of Deregulation Driven by IgH Locus Enhancers as Models of B-Cell Lymphomagenesis.
Front Immunol
April 2021
Inserm U1262, UMR CNRS 7276, Equipe Labellisée LIGUE 2018, Université de Limoges, Limoges, France.
Article Synopsis
- Chromosomal translocations that connect oncogenes to IgH locus enhancers are linked to B-cell cancers.
- There are two key IgH enhancers: the E enhancer, which supports early B-cell maturation, and the 3' regulatory region (3'RR), which manages later stages like IgH transcription and class switching.
- Mouse models have been crucial in studying the roles of these enhancers in B-cell lymphoma, though they have specific strengths and weaknesses that affect our understanding of similar processes in humans.
IgM monoclonal gammopathy with heavy-and-light-chain amyloidosis resembling fibrillary glomerulonephritis determined by tandem mass spectrometry: a case report.
BMC Nephrol
May 2020
Department of Nephrology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan.
Article Synopsis
- Fibrillary glomerulonephritis (FGN) can be difficult to distinguish from amyloidosis due to overlapping symptoms, but recent advancements like laser microdissection followed by mass spectrometry (LMD/MS) can aid in accurate diagnosis.
- A 75-year-old man initially diagnosed with FGN based on kidney biopsy and lab findings was later confirmed to have immunoglobulin heavy-and-light-chain amyloidosis (AHL) after LMD/MS testing revealed a positive Congo red stain.
- The case highlights the importance of using LMD/MS in atypical cases where conventional tests might lead to misdiagnosis, especially when there are unusual pathological features like thicker fibrils.
[Genetic diagnosis of patients with primary agammaglobulinemia treated at third level peruvian centers].
Rev Peru Med Exp Salud Publica
July 2020
Sociedad Peruana de Inmunología (SPI). Lima, Perú.
Article Synopsis
- Primary agammaglobulinemia is a condition caused by changes in B cells, leading to low antibody levels and frequent infections, confirmed by genetic testing.
- In Peru, there's a lack of research on this condition and its genotypes, prompting a new study.
- The study identified 20 patients with mutations in the BTK gene, primarily X-linked agammaglobulinemia, including both hereditary and new mutations.
Autosomal recessive agammaglobulinemic patient with a novel large deletion in IGHM presenting with mild clinical phenotype.
Clin Immunol
January 2020
Division of Pediatric Allergy and Immunology, Marmara University, Istanbul, Turkey; Istanbul Jeffrey Modell Center for Primary Immunodeficiencies, Turkey. Electronic address:
A Targeted Mass Spectrometry Strategy for Developing Proteomic Biomarkers: A Case Study of Epithelial Ovarian Cancer.
Mol Cell Proteomics
September 2019
‡Department of Biology, Institute of Molecular Systems Biology, ETH Zurich, 8093 Zurich, Switzerland; §§§Faculty of Science, University of Zurich, 8057 Zurich, Switzerland.
Article Synopsis
- Protein biomarkers are essential for early detection and management of epithelial ovarian cancer, improving patient outcomes and monitoring treatment responses.
- The discovery of these biomarkers is challenging due to the need for reliable assays that can quantify proteins in complex biological samples like blood plasma.
- A targeted mass spectrometry approach, specifically selected reaction monitoring (SRM), was used to identify and quantify potential biomarkers, resulting in a 5-protein signature that outperformed standard CA125 measurements in differentiating ovarian cancer patients from healthy individuals.