In vitro selection and characterization of a DNA aptamer targeted to Prorocentrum minimum-A common harmful algae.

Prorocentrum minimum is a common diarrhetic shellfish toxins-producing marine microalga that may seriously endanger marine resources and cause great economic losses. The development of a novel rapid detection technique is of great importance for the prevention and control of the damage caused by P. minimum.

Quasi-synchronization of fractional-order multi-layer networks with mismatched parameters via delay-dependent impulsive feedback control.

The paper is devoted to investigating the quasi-synchronization issue of fractional-order multi-layer networks with mismatched parameters under delay-dependent impulsive feedback control. It is worth highlighting that fractional-order multi-layer networks with mismatched parameters, as the extension model for single-layer or two-layer ones, are constructed in this paper. Simultaneously, the intra-layer and inter-layer couplings are taken into consideration, which is more general and rarely considered in discussions of network synchronization.

Research progress of whole-cell-SELEX selection and the application of cell-targeting aptamer.

Background: Aptamers refer to the artificially synthesized nucleic acid sequences (DNA/RNA) that can bind to a wide range of targets with high affinity and specificity, which are generally generated from systematic evolution of ligands by exponential enrichment (SELEX). As a novel method of aptamers screening, whole-cell-SELEX (WC-SELEX) has gained more and more attention in many fields such as biomedicine, analytical chemistry, and molecular diagnostics due to its ability to screen multiple potential aptamers without knowing the detailed structural information of target molecules.

Methods And Results: In recent years, with the deepening of research on application of aptamers, the traditional WC-SELEX cannot meet the practical application because of long experimental period, complicated operation process and low specificity, etc.

Environmental Penalties, Investor Attention and Stock Market Reaction: Moderating Roles of Air Pollution and Industry Saliency.

Despite the importance of environmental penalties in environmental enforcement, how and under what situations they impact stock market reaction is still unclear. Drawing on the theories of expectancy violation and attention driven, a conceptual model is built to explore how environmental penalty influences stock market reaction through investor attention. Furthermore, it is explored that the air pollution and industry saliency facilitate the indirect relationship between environmental penalty and investor attention.

Rapid detection of by PCR combined with dot lateral flow strip.

Unlabelled: In this study a novel technique referred to as PCR combined with dot lateral flow strip (PCDS) is proposed and its application to the detection of harmful microalgae was explored. For this purpose, using as a test algal species, PCR targeting the D1-D2 region of large subunit ribosomal gene of this alga was performed with the tagged specific primers. The amplicons were then analyzed with the manually prepared dot lateral flow strip, and the strip could produce a test dot and a control dot that are naked-eye detectable, indicating the successful establishment of PCDS.

Mass spectrometric observation on free radicals during electrooxidation of dopamine.

It has been challenging to directly observe the o-semiquinone radicals and transient intermediates produced during the oxidation of dopamine (DA). To achieve this goal, we developed an electrochemistry-neutral reionization-mass spectrometry (EC-NR-MS) technique for online studying the electrooxidation process of DA. The EC-NR device mainly composed by a self-designed EC flow cell, a sonic spray ionization source, a heating tube, an ion deflector and an electrospray ionization source.

High-Sensitivity PtSe Surface Plasmon Resonance Biosensor Based on Metal-Si-Metal Waveguide Structure.

PtSe as a novel TMDCs material is used to modify the traditional SPR biosensors to improve the performance. On this basis, this research proposes a metal-Si-metal waveguide structure to further improve the performance of the biosensor. In this study, we not only studied the effects of waveguide structures containing different metals on the performance of biosensor, but also discussed the performance change of the biosensor with the change of PtSe thickness.

A review of the current and emerging detection methods of marine harmful microalgae.

In recent years, the scale and frequency of outbreaks of harmful algal blooms (HABs) have increased year by year due to the intensification of seawater eutrophication and global climate change. HABs have become a global marine ecological and environmental problem, which poses a serious threat to human health, marine ecological security, and economic development. The establishment of detection technology for harmful microalgae is fundamental to the early warning and prevention of HABs.

Metabolomics reveals the mechanism of Antarctic yeast Rhodotorula mucliaginosa AN5 to cope with cadmium stress.

Heavy metal pollution in Antarctica has far exceeded expectations. Antarctic yeast is widely present in polar marine environment. The mechanisms of metabolomics effect of heavy metal on polar yeast have not been reported previously.

Bipartite synchronization of signed networks via aperiodically intermittent control based on discrete-time state observations.

In this paper, bipartite synchronization of signed networks with stochastic disturbances via aperiodically intermittent control is investigated. The aperiodically intermittent control presented is based on discrete-time state observations rather than continuous-time ones. To formulate signed networks and exhibit the competitive relation, a structurally balanced signed network is built and all the units are divided into two subcommunities.

Core-shell structured nanocomposites formed by silicon coated carbon nanotubes with anti-oxidation and electromagnetic wave absorption.

The silicon coated Carbon nanotubes (CNTs) nanocomposite (CNTs@Si) with a shell structure was successfully synthesized by a simple chemical vapor deposition (CVD) method. In this work, the CNTs@Si is not only introduced as a structural material providing oxidation performance, but also as an extremely effective electromagnetic wave (EMW) absorption nanocomposite. Dielectric characteristics EMW absorption properties within the frequency range of 2-18 GHz of CNTs@Si were studied, and the oxidation resistance of CNTs@Si was characterized.

Correction: Mass spectrometric detection of fleeting neutral intermediates generated in electrochemical reactions.

[This corrects the article DOI: 10.1039/D1SC01385H.].

Mass spectrometric detection of fleeting neutral intermediates generated in electrochemical reactions.

Towards the goal of on-line monitoring of transient neutral intermediates during electrochemical reactions, an electrochemistry-neutral reionization-mass spectrometry (EC-NR-MS) technique was developed in this work. The EC-NR setup consisted of a customized EC flow cell, a sonic spray ionization source, a heating tube, an ion deflector and an electrospray ionization source, which were respectively used for the precise control of the electrochemical reaction, solution nebulization, droplet desolvation, ion deflection and neutral intermediate ionization. Based on the EC-NR-MS approach, some long-sought neutral radicals including TPrA˙, DBAE˙ and TEOA˙, which belong to important reductive intermediates in electrochemiluminescence (ECL) reactions, were successfully identified which helps to clarify the previously unproven ECL reaction mechanism.

Understanding of remora's "hitchhiking" behaviour from a hydrodynamic point of view.

Symbiotic relationships have developed through natural evolution. For example, that of the remora fish attached to the body of a shark. From the remora's perspective, this could be associated to an increased hydrodynamic efficiency in swimming and this needs to be investigated.

Rapid and sensitive detection of Karlodinium veneficum by a novel double-nick rolling circle amplification.

Harmful algal blooms caused by Karlodinium veneficum recently occurred with high incidence, posing a serious threat to the marine ecological environment, public health, and mariculture. It is therefore rather vital to establish a method for rapid detection of K. veneficum.

Factors Associated With Health-Related Quality of Life of Parents Who Lost Their Only Child: A Cross-Sectional Study in Central China.

The number of families who lost their only child was over one million in 2012 in China, and it is growing rapidly every year. Without their only child, they will inevitably worry about their health and life for their later years. It is important to explore the quality of life and influencing factors of the parents.

Easy detection of karlodinium veneficum using PCR-based dot chromatography strip.

In this study, a novel detection method by PCR-based dot chromatography strip (PDCS) is proposed. To investigate the application of PDCS in the detection of harmful microalgae, the internal transcribed spacer sequence of Karlodinium veneficum, one of the most common bloom-forming species, was cloned and sequenced to design and screen specific primers with tag sequences and probes, including gold nanoparticle probe, test probe, and control probe. The PDCS was prepared manually, and PCR amplicons prepared from the genomic DNA of K.

SWL-1 Reverses Fluconazole Resistance in by Regulating the Glycolytic Pathway.

is a ubiquitous clinical fungal pathogen. Prolonged use of the first-line antifungal agent fluconazole (FLC) has intensified fungal resistance and limited its effectiveness for the treatment of fungal infections. The combined administration of drugs has been extensively studied and applied.

Full three-dimensional segmentation and quantification of tumor vessels for photoacoustic images.

Quantitative analysis of tumor vessels is of great significance for tumor staging and diagnosis. Photoacoustic imaging (PAI) has been proven to be an effective way to visualize comprehensive tumor vascular networks in three-dimensional (3D) volume, while previous studies only quantified the vessels projected in one plane. In this study, tumor vessels were segmented and quantified in a full 3D framework.

Stability analysis and Hopf bifurcation in a diffusive epidemic model with two delays.

A diffusive epidemic model with two delays subjecting to Neumann boundary conditions is considered. First we obtain the existence and the stability of the positive constant steady state. Then we investigate the existence of Hopf bifurcations by analyzing the distribution of the eigenvalues.

Detection of Prorocentrum minimum by hyperbranched rolling circle amplification coupled with lateral flow dipstick.

A novel method referred to as hyperbranched rolling circle amplification (HRCA) coupled with lateral flow dipstick (LFD) (HRCA-LFD) here was developed for specific, sensitive, rapid, and simple detection of Prorocentrum minimum. HRCA-LFD relies on a padlock probe (PLP) consisting of a common ligation sequence, two terminal sequences that complement the target DNA, and a manually designed detection probe (LFD probe). The two terminal sequences of the PLP were designed against the species-specific sites of the large subunit ribosomal DNA (LSU rDNA) D1-D2 region of P.

Establishment of double probes rolling circle amplification combined with lateral flow dipstick for rapid detection of Chattonella marina.

Chattonella marina is one of the main algae that could cause harmful algal blooms. It has killed a large number of cultured fish in coastal areas of many countries, causing serious economic losses. Therefore, it is necessary to establish a method that can specifically detect C.

Highly reversible oxygen redox in layered compounds enabled by surface polyanions.

Oxygen-anion redox in lithium-rich layered oxides can boost the capacity of lithium-ion battery cathodes. However, the over-oxidation of oxygen at highly charged states aggravates irreversible structure changes and deteriorates cycle performance. Here, we investigate the mechanism of surface degradation caused by oxygen oxidation and the kinetics of surface reconstruction.

Effect of workplace ostracism on emotional exhaustion and unethical behaviour among Chinese nurses: A time-lagged three-wave survey.

Aim: This study examined the following: (a) the impact of nurses' workplace ostracism on unethical behaviour; (b) mediating role of emotional exhaustion between nurses' workplace ostracism and unethical behaviour; and (c) moderating effect of hostile attribution bias.

Background: While unethical behaviour is a workplace phenomenon that can negatively influence the sustainable development of settings, few nurse studies have explored it. This study identified an interpersonal antecedent of unethical behaviour: workplace ostracism.

Aperiodically intermittent control for exponential bipartite synchronization of delayed signed networks with multi-links.

This paper investigates the exponential bipartite synchronization of a general class of delayed signed networks with multi-links by using an aperiodically intermittent control strategy. The main result is a set of sufficient conditions for bipartite synchronization that depend on the network's topology, control gain, and the maximum proportion of rest time. An application to Chua's circuits is then considered, and some numerical simulation results are presented.