Single-cell and spatial transcriptomics reveal apelin/APJ pathway's role in microvessel formation and tumour progression in hepatocellular carcinoma.

The apelin receptor (APJ) is a key player in tumour angiogenesis, but its role in hepatocellular carcinoma (HCC) remains unclear. This study aims to elucidate the function of the apelin/APJ pathway in HCC using a multi-omics approach and identify potential therapeutic biomarkers. Differentially expressed genes related to the apelin/APJ axis were identified from bulk transcriptomics to reveal HCC-associated disparities.

Facile Synthesis and Characterization of Fluorescent Polystyrene Nanospheres for Homogeneous Light-Initiated Chemiluminescence Immunoassay.

Homogeneous light-initiated chemiluminescence technology (LICA) is widely used in clinical diagnostics due to the advantages of high sensitivity, minimal reagent usage, and no need for washing. Luminescent microspheres receive singlet oxygen emitted by photosensitive microspheres to generate optical signals. Therefore,O-initiated luminescent nanospheres are crucial, but there are few reports on the preparation of O-initiated luminescent nanospheres.

AINTEGUMENTA-LIKE genes regulate reproductive growth and bud dormancy in Platanus acerifolia.

Platanus acerifolia AIL genes PaAIL5a/b and PaAIL6b participate in FT-AP1/FUL-AIL pathways to regulate bud dormancy. In addition, PaAIL6a/b can promote flowering, and PaAIL5b and PaAIL6b affect floral development. Bud dormancy and floral induction are essential processes for perennial plants, they are both regulated by photoperiod, temperature, and hormones, indicating the existence of common regulators for both processes.

Graded Progressive Home-Based Resistance Combined with Aerobic Exercise in Community-Dwelling Older Adults with Sarcopenia: A Randomized Controlled Trial.

Purpose: This randomized parallel controlled trial aimed to determine the effectiveness of graded progressive home-based resistance combined with aerobic exercise in improving physical fitness in community-dwelling older adults with sarcopenia.

Patients And Methods: Community-dwelling older adults (≥60 years) with sarcopenia were randomly assigned to the intervention group (IG), receiving 12 weeks of graded progressive home-based resistance and aerobic exercise training, and the control group (CG), maintaining lifestyle unchanged. The primary outcomes were knee extensor muscle strength and the six-minute walk distance (6MWD).

directed evolution of AtMP1 antimicrobial peptide to improve anticancer activity.

Various studies have demonstrated that directed evolution is a powerful tool in enhancing protein properties. In this study, directed evolution was used to enhance the efficacy of synthesised AtMP1 antimicrobial peptides (AMPs) in inhibiting the proliferation of cancer cells. The modification of antimicrobial peptides (AMPs) and prediction of peptide properties using bioinformatic tools were carried out using four databases, including ADP3, CAMP-R3, AMPfun, and ANTICP.

Joint effect of docosahexaenoic acid intake and tobacco smoke exposure on learning disability in children and adolescents: a cross-sectional study from the NHANES database.

Background: Docosahexaenoic acid (DHA) has been reported to be associated with the children's neurodevelopment, who may be exposed to tobacco smoke simultaneously. The evidence about joint effect of DHA intake and tobacco smoke exposure on children and adolescents' learning disabilities (LD) was limited. The objective of this study was to assess the joint effect of DHA intake and tobacco smoke exposure on children and adolescents' LD.

[Biological and genome characteristics of a novel broad host-range phage ΦEP1 infecting enteroinvasive ].

We analyzed the biological and genome characteristics of a phage infecting enteroinvasive (EIEC), aiming to provide resources and a reference for the prevention and treatment of EIEC. With the EIEC preserved in our laboratory as the host bacterium, one strain of phage was isolated from the effluent sample from a chicken farm in Huzhou, Zhejiang and named ΦEP1. The titer, optimal multiplicity of infection, one-step growth curve, temperature, pH value, chloroform and bile salt sensitivity of ΦEP1 were determined by the double-layer agar plate method.

How attentive is a student in class? A concentration evaluation method based on head Euler angle.

How attentive is a student in class? It is an interesting and challenging question in the field of education research. In this paper, a concentration evaluation method based on head Euler angle is proposed. First, a fine large-small-interval video sampling strategy is advocated and concepts of dynamic threshold and action amplitude coefficient are defined, which are fused together to get the head up rate.

Network Analysis of Pain Catastrophizing, Self-Efficacy, and Kinesiophobia Among Patients After Total Knee Arthroplasty: A Cross-Sectional Study.

Background: Kinesiophobia is common in patients after total knee arthroplasty and is an important risk factor for post-operative recovery outcomes. Little is known about the complex intercorrelations between different components of pain catastrophizing, self-efficacy, and kinesiophobia. This study aimed to identify the central components of kinesiophobia and to explore the interconnectedness between components of pain catastrophizing, self-efficacy, and kinesiophobia.

The role of liquid-liquid phase separation in the disease pathogenesis and drug development.

Misfolding and aggregation of specific proteins are associated with liquid-liquid phase separation (LLPS), and these protein aggregates can interfere with normal cellular functions and even lead to cell death, possibly affecting gene expression regulation and cell proliferation. Therefore, understanding the role of LLPS in disease may help to identify new mechanisms or therapeutic targets and provide new strategies for disease treatment. There are several ways to disrupt LLPS, including screening small molecules or small molecule drugs to target the upstream signaling pathways that regulate the LLPS process, selectively dissolve and destroy RNA droplets or protein aggregates, regulate the conformation of mutant protein, activate the protein degradation pathway to remove harmful protein aggregates.

Efficiency and mechanism of controlling phosphorus release from sediment using a biological aluminum-based P-inactivation agent.

Eutrophication is a significant challenge for surface water, with sediment phosphorus (P) release being a key contributor. Although biological aluminum-based P-inactivation agent (BA-PIA) has shown effectiveness in controlling P release from sediment, the efficiency and mechanism by BA-PIA capping is still not fully understood. This study explored the efficiency and mechanism of using BA-PIA capping controlling P release from sediment.

Photo-induced difluoroalkylation/cyclization of alkyne ketones: a novel strategy to access difluoroalkyl thiofavones.

A photo-induced electron donor-acceptor (EDA) complex enabled tandem reaction of alkyne ketones a radical difluoroalkylation/cyclization cascade sequence is reported. The EDA complex plays a key role, and the CBr bond homolysis process may also be involved for this transformation. Varieties of difluoroalkyl-substituted thiofavones can be smoothly assembled in moderate to good yields under photocatalyst-, metal- and oxidant-free conditions, thus offering potential applications for pharmaceutical research.

[Five-year survival analysis of gastric cancer from population-based cancer registration data in Zhejiang province, China].

To analyze epidemiology of gastric cancer five-year survival distribution in Zhejiang population-based cancer registration. The follow-up data of registrated gastric cancer cases diagnosed from 2008 to 2019 in 22 national cancer registry areas of Zhejiang Province were collected and divided into three diagnostic periods: 2008-2011, 2012-2015 and 2016-2019 to calculate five-year observed survival rates (OSRs), five-year relative survival rates (RSRs) and five-year age-standardized relative survival rates (ARSRs). The distribution of population characteristics (including gender, urban/rural, age group and occupation) and clinical characteristics (including the highest diagnostic institution, sub-site, pathological type and degree of differentiation) of gastric cancer survival rates in each period were analysed.

Effects and mechanisms of aquatic landscape plants on the removal of veterinary antibiotics from hydroponic solutions.

Four aquatic landscape plants and three veterinary antibiotics were selected to construct a hydroponic test system to analyze the tolerance, removal effect and mechanism of antibiotics. The results indicated that antibiotic concentrations from 0 to 100 μg·L promoted plant heights and leaf chlorophyll contents, while antibiotics at concentrations > 100 μg·L had inhibitory effects. The ability of different plants to remove antibiotics was L.

Pulse signal may be a key bridge connecting cardiology and pulse diagnosis of TCM.

Objective: This study extracts atherosclerosis indices from six channels of and discusses the data distribution, aims to explore the connection between cardiology of modern medicine (MM) and traditional Chinese medicine (TCM) pulse diagnosis.

Methods: We use a device capable of simultaneously collecting the pulse signals of the , and to test the population participating in routine physical examinations. Firstly, we collected pressure pulse waves from six channels of of 1045 healthy subjects (578 men and 467 women, average age=37.

Integrated multi-omics reveals the relationship between growth performance, rumen microbes and metabolic status of Hu sheep with different residual feed intakes.

Residual feed intake (RFI) is a metric that provides a more accurate measure of feed efficiency. The lower the RFI, the higher the feed efficiency. The changes in the host microbiome and metabolome contribute to the greater feed efficiency of low RFI (LRFI) animals.

High Efficiency Removal Performance of Tetracycline by Magnetic CoFeO/NaBiO Photocatalytic Synergistic Persulfate Technology.

The widespread environmental contamination resulting from the misuse of tetracycline antibiotics (TCs) has garnered significant attention and study by scholars. Photocatalytic technology is one of the environmentally friendly advanced oxidation processes (AOPs) that can effectively solve the problem of residue of TCs in the water environment. This study involved the synthesis of the heterogeneous magnetic photocatalytic material of CoFeO/NaBiO via the solvothermal method, and it was characterized using different characterization techniques.