R2R3 MYB Transcription Factors Involved in Flower Petal Pigmentation via Regulating Anthocyanin Synthesis in Rhododendron simsii.

Azaleas (Rhododendron simsii) are popular ornamental woody plants known for their bright colors; however, very limited studies have been reported on the process of flower petal pigmentation. In this study, we found significant differences in the anthocyanin contents of petals from different colored azaleas, and the results of quantitative real-time PCR indicated that the R2R3 MYB genes, RsMYB12, RsMYB90, and RsMYB123, showed significant expression changes during the petal coloration in azalea petals; therefore, we hypothesized that RsMYB12, RsMYB90, and RsMYB123 might involve in the coloring process of azalea petals by regulating anthocyanin synthesis. This work provides insights into the underlying mechanisms of petal pigmentation in R.

Expression pattern of cancer-associated cellular senescence genes in clear cell renal cell carcinoma distinguishes tumor subclasses with clinical implications.

Clear cell renal cell carcinoma (ccRCC) is a highly lethal subtype of renal cancer. Accumulating evidence suggests cellular senescence impacts tumor development and progression. This study aimed to identify ccRCC subtypes based on a cellular senescence gene signature and assess their clinical relevance.

[Study on the relationship between occupational exposure characteristics and musculoskeletal disorders in 29 industries or workforce groups in China].

To explore the occupational contact characteristics of work-related musculoskeletal disorders (WMSDs) among key occupational groups in China, assess the impact of occupational factors on the occurrence of WMSDs, and propose corresponding preventive strategies. Between January 1, 2018, and December 31, 2023, a cross-sectional epidemiological survey method was employed to retrospectively investigate the occurrence of WMSDs and related occupational factors. Valid questionnaire data from 88609 on-the-job workers in 441 enterprises across 29 industries or job types in seven regions of China were collected, and the relationship between WMSDs and various occupational factors was discussed using multifactorial logistic regression analysis.

Directed evolution of glutamate decarboxylase B for enhancing its enzyme activity towards nearly neutral pHs based on error-prone PCR.

Glutamate decarboxylases (GADs) can catalyze the conversion of l-glutamate to γ-aminobutyric acid (GABA), while consuming one H. However, the GADs found so far are catalytically active in the pHs of 3.8-5.

Existence and significance of anti-HLA-C autoantibodies to primary and persistent platelet transfusion refractoriness in patients with hematologic disorders: a retrospective study from a single centre.

Objectives: Platelet transfusion refractoriness (PTR) is a frustrating clinical problem, and primary and persistent (P/P) PTR who experienced persistent PTR since the first transfusion was failed to be well recognized. This study aims to investigate the incidence and risk factors for P/P PTR.

Methods: Patients with hematologic disorders who underwent HLA high-resolution genotyping and donor-specific HLA antibody or panel reactive antibody (PRA) testing between January 2019 and March 2023 were reviewed.

Research Progress on Using Modified Hydrogel Coatings as Marine Antifouling Materials.

The adhesion of marine organisms to marine facilities negatively impacts human productivity. This phenomenon, known as marine fouling, constitutes a serious issue in the marine equipment industry. It increases resistance for ships and their structures, which, in turn, raises fuel consumption and reduces ship speed.

Relationships between daily emotional experiences and smartphone addiction among college students: moderated mediating role of gender and mental health problems.

Introduction: The theoretical model of smartphone addiction highlights the role of emotional factors in fostering addictive behaviors. However, most research has focused on long-term emotional states and pathologies, often overlooking the immediate effects of daily emotional fluctuations on smartphone usage and their mechanisms.

Methods: Our study employed an online survey and a moderated parallel mediation model to explore how daily emotional experiences influence smartphone addiction among college students.

Visible-Light Induced and Iron Peroxo-Promoted Radical Difunctionalization of Alkene for the Synthesis of β-Ketosulfone and α-Chloroketone.

In this work, a switchable synthesis of β-ketosulfone and α-chloroketone through a radical difunctionalization of alkenes is reported. The transformation works well under iron peroxo species/photoredox dual catalysis and an open-flask atmosphere, and the reaction is highlighted with good yields and a broad reaction scope. Mechanism studies show that the reaction is initiated by a formal [4 + 2] cyclization of the sulfonyl radical in a regioselective manner.

Prevalence and factor associated with depressive symptoms in patients with osteoarthritis: A cross-sectional study.

Objective: Osteoarthritis (OA) is a prevalent degenerative joint condition. Among OA patients, depressive symptoms are the most frequent psychiatric disorder, negatively impacting both prognosis and quality of life. This study analyzed the independent factors associated with the development of depressive symptoms in patients with OA and constructed a nomogram to assess the risk of developing depressive symptoms.

Corrigendum: Age effects of Moso bamboo on leaf isoprene emission characteristics.

[This corrects the article DOI: 10.3389/fpls.2023.

Ferric nitrene-promoted anti-Markovnikov ring-opening of epoxides and nucleophilic functionalization of benzylic C-H bonds under photo-irradiation.

This paper describes a ferric nitrene/photoredox dual-catalyzed anti-Markovnikov ring-opening of epoxides under neutral conditions for providing α-substituted acetophenones. A DFT-based calculation supported the reaction regioselectivity. The catalytic system could also be applied to the formation of C-O and C-N bonds nucleophilic functionalization of benzylic C-H bonds.

Urban ecosystem services, ecological security patterns and ecological resilience in coastal cities: The impact of land reclamation in Macao SAR.

Land reclamation from the sea is a common practice to create territorial space and accommodate urbanisation in coastal cities. However, previous studies did not adequately examine the changes in ecosystem service values, the spatial transformation of the ecological network and the ecological resilience at an urban scale in the context of land reclamation. This study uses Macao SAR, a fully urbanised city with two-thirds of its land reclaimed from the sea, as a case study.

Multi-perspective kinetic analysis of plastic blends under thermogravimetric pyrolysis conditions.

Plastic blends were co-pyrolyzed under non-isothermal conditions in a thermogravimetric (TG) analyzer. The co-pyrolysis characteristics and kinetic triplet, i.e.

Constructing Dynamic Macropores in Thermo-Responsive Hydrogel Actuator for Large-Deformable Gripper.

Poly(N-isopropyl acrylamide) (PNIPAm)-based smart hydrogels are widely employed in emerging applications such as drug delivery and tissue engineering, because their lower critical solution temperature (LCST) is close to physiological conditions. However, the dense chain collapse during the thermo-responsive phase transition restricts water diffusion, resulting in limited volumetric change. Here, a pure PNIPAm hydrogel that achieves a large-scale volume transition by incorporating PNIPAm microgels, is presented.

Photoinduced Radical Relay Reaction of 2-Methylthiolated Phenylacetylenes/Alkynones Initiated by Electron Donor-Acceptor Complexes.

A method was found to construct sulfur-containing five- and six-membered heterocyclic alkyl sulfonyl compounds by using visible light and free radicals activated and/or generated by EDA complexes/homolytic cleavage as initiators to stimulate the relay reaction of alkynes/alkynones. This method puts forward a new strategy to initiate alkyl sulfonation of alkynes/alkynones with only a catalytic amount of the initiator. This strategy of generating the initiator by EDA complex activation/homolytic cleavage provides a new idea for the following substances that must be excited.

TssL2 of T6SS2 is required for mobility, biofilm formation, wrinkly phenotype formation, and virulence of Vibrio parahaemolyticus SH112.

Type VI secretion system 2 (T6SS2) of Vibrio parahaemolyticus is required for cell adhesion and autophagy in macrophages; however, other phenotypes conferred by this T6SS have not been thoroughly investigated. We deleted TssL2, a key component of T6SS2 assembly, to explore the role of the T6SS2 in environmental adaptation and virulence. TssL2 deletion reduced Hcp2 secretion, suggesting that TssL2 played an important role in activity of functional T6SS2.

Development and Characterization of Sustainable Cement-Free Controlled Low Strength Material Using Titanium Gypsum and Construction Waste Soil.

This study investigates the utilization of titanium gypsum (TG) and construction waste soil (CWS) for the development of sustainable, cement-free Controlled Low Strength Material (CLSM). TG, combined with ground granulated blast furnace slag, fly ash, and quicklime, serves as the binder, while CWS replaces natural sand. Testing thirteen mixtures revealed that a CWS replacement rate of over 40% controls bleeding below 5%, with a water-to-solid ratio between 0.

RNA-Seq Transcriptomics and iTRAQ Proteomics Analysis Reveal the Dwarfing Mechanism of Blue Fescue ().

Blue fescue is a widely used ornamental grass because of its strong ecological adaptability. To maintain the optimal ornamental plant shape, blue fescue requires many nutrients and labor. Using dwarf varieties with slow growth is an effective way to fulfill these requirements.

Family participation in dignity interventions for patients with cancer receiving palliative care: a scoping review protocol.

Introduction: The global burden of cancer is escalating, with Asia accounting for over half of cancer-related deaths worldwide. As cancer often diminishes patients' quality of life and sense of dignity, dignity-related interventions have gained prominence in palliative care for patients with cancer. However, a more in-depth exploration of the involvement of families, as the fundamental social units in Confucian Asian cultures, and cultural considerations is currently lacking.

The effectiveness of a nurse-led home-based cardiac rehabilitation based on instant information intervention in the prognosis of patients with TAVR: A randomized controlled trial protocol.

Background: Patients with poor prognosis after TAVR surgery require high-quality cardiac rehabilitation. Digital home-based rehabilitation based on real-time information intervention can improve outcomes. However, the parameters of the text message intervention, such as text message content, intervention duration, text message frequency, and other parameters, may vary.

Enhanced autophagy and cholesterol efflux in mouse mesenchymal stem cells infected with H37Rv compared to H37Ra.

Autophagy, metabolism, and associated signaling pathways play critical roles in bacterial survival within mammalian cells and influence the immunopathogenesis of infections. Mesenchymal stem cells (MSCs) are important host cells during Mycobacterium tuberculosis (Mtb) infection, yet how autophagy, metabolism, and related pathways are modulated in MSCs infected with the virulent H37Rv or the attenuated H37Ra strain of Mtb remains poorly understood. In this study, we utilized RNA-Seq screening, qRT-PCR, and Western Blotting to investigate the differences in these processes between H37Rv and H37Ra infections.

Unlocking the potential of chitosan in immunoassay sensor.

Using new materials to improve detection accuracy and efficiency is important to broaden the application of immunoassay sensors. Among numerous materials for improving sensors, chitosan, as a natural polysaccharide, has excellent biocompatibility, mechanical adjustability, stimulation sensitivity and porous structure, which holds significant potential for enhancing the performance of immunoassay sensors. However, at present, there are few systematic reviews on the application of chitosan in immune sensors.

School Climate and School Identification as Determinants of Internet Gaming Disorder Among Chinese Adolescent Internet Gamers: Cross-Sectional Mediation Study.

Background: School climate and school identification are important features of the school environment and potential determinants of adolescent internet gaming disorder (IGD).

Objective: This novel study investigated their joint effects on IGD and related mediation mechanisms via the interpersonal factor of teacher-student relationship and the individual factors of academic stress and anxiety.

Methods: A large-scale cross-sectional study was conducted among adolescent internet gamers of junior, senior, and vocational middle schools in Taizhou City, China, from February to March 2022 (N=5778).