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MALDI-TOF nucleic acid mass spectrometry for simultaneously detection of fourteen porcine viruses and its application.

J Virol Methods

September 2024

Hangzhou Customs Technical Center, Hangzhou 311202, China; Zhejiang Academy of Science & Technology for Inspection & Quarantine, Hangzhou 310016, China. Electronic address:

Article Synopsis
  • Mixed infections from various viruses significantly impact swine health and the livestock economy, making effective detection methods essential.
  • This study introduces a new method that combines single-base extension PCR with MALDI-TOF MS to identify 14 key porcine viruses accurately and simultaneously.
  • The new diagnostic assay demonstrated high sensitivity and reliability, achieving 97.9% agreement with traditional qPCR testing, highlighting its potential for enhancing virus surveillance and disease control in swine.
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