Nuclease activity gives an edge to host-defense peptide piscidin 3 over piscidin 1, rendering it more effective against persisters and biofilms.

Host-defense peptides (HDPs) feature evolution-tested potency against life-threatening pathogens. While piscidin 1 (p1) and piscidin 3 (p3) are homologous and potent fish HDPs, only p1 is strongly membranolytic. Here, we hypothesize that another mechanism imparts p3 strong potency.

Sensory cue-combination in the context of newly learned categories.

A large body of prior research has evaluated how humans combine multiple sources of information pertaining to stimuli drawn from continuous dimensions, such as distance or size. These prior studies have repeatedly demonstrated that in these circumstances humans integrate cues in a near-optimal fashion, weighting cues according to their reliability. However, most of our interactions with sensory information are in the context of categories such as objects and phonemes, thereby requiring a solution to the cue combination problem by mapping sensory estimates from continuous dimensions onto task-relevant categories.

aCORN: An experiment to measure the electron-antineutrino correlation coefficient in free neutron decay.

We describe an apparatus used to measure the electron-antineutrino angular correlation coefficient in free neutron decay. The apparatus employs a novel measurement technique in which the angular correlation is converted into a proton time-of-flight asymmetry that is counted directly, avoiding the need for proton spectroscopy. Details of the method, apparatus, detectors, data acquisition, and data reduction scheme are presented, along with a discussion of the important systematic effects.

Noise correlations in the human brain and their impact on pattern classification.

Multivariate decoding methods, such as multivoxel pattern analysis (MVPA), are highly effective at extracting information from brain imaging data. Yet, the precise nature of the information that MVPA draws upon remains controversial. Most current theories emphasize the enhanced sensitivity imparted by aggregating across voxels that have mixed and weak selectivity.

Assessment of the feeding Swallowing Impact Survey as a quality of life measure in children with laryngeal cleft before and after repair.

Objective: To cross-validate the Feeding Swallowing Impact Survey (FSIS), a quality of life instrument, specifically to a subpopulation of children who aspirate due to laryngeal cleft.

Introduction: The FSIS is a recently validated instrument used to describe caregiver quality of life (QOL) in children with aspiration due to various causes. To cross-validate the FSIS specifically to the subpopulation of children who aspirate due to laryngeal cleft, we tested the hypotheses that caregivers would report significant different scores form baseline if their children improved at the one year mark postintervention due to either successful conservative or surgical measures (discriminant validity) and would not report significant differences in their FSIS reporting if there was no change in their child's aspiration at the one year mark post intervention (convergent validity).

Gender and gender typicality in autobiographical memory: A replication and extension.

Gender differences in autobiographical memory (AM) are commonly reported, but often inconsistent across studies using varied measurement techniques. The current study aimed to provide more clarity to where and why gender differences emerge by carefully controlling for factors hypothesised to be relevant to gender; thus, it tested shorter and longer term AM using both narrative and questionnaire measures, and further tested whether subscription to feminine-typical traits mediated these differences. Results demonstrate that women's memory narratives score higher than men's on measures of affect, connection to others, factual and interpretive elaboration, and thematic coherence.

Complete Biosynthesis of Anthocyanins Using Polycultures.

Fermentation-based chemical production strategies provide a feasible route for the rapid, safe, and sustainable production of a wide variety of important chemical products, ranging from fuels to pharmaceuticals. These strategies have yet to find wide industrial utilization due to their inability to economically compete with traditional extraction and chemical production methods. Here, we engineer for the first time the complex microbial biosynthesis of an anthocyanin plant natural product, starting from sugar.

Naringenin-responsive riboswitch-based fluorescent biosensor module for Escherichia coli co-cultures.

The ability to design and construct combinatorial synthetic metabolic pathways has far exceeded our capacity for efficient screening and selection of the resulting microbial strains. The need for high-throughput rapid screening techniques is of upmost importance for the future of synthetic biology and metabolic engineering. Here we describe the development of an RNA riboswitch-based biosensor module with dual fluorescent reporters, and demonstrate a high-throughput flow cytometry-based screening method for identification of naringenin over producing Escherichia coli strains in co-culture.

Subjective social status and premedical students' attitudes towards medical school.

Although students from lower socioeconomic backgrounds are more likely to depart from the medical education pipeline, little is known about how premedical students' social origins influence the adversities they face, the resources they possess, or the internal struggles they confront in deciding whether or not to pursue a career in medicine. Using original data collected from premedical students at a flagship state university in the Midwest (N = 364), this study explores how subjective social status shapes students' reservations about medical school attendance and their perceptions of external pressure to pursue a career in medicine. Status-based discrepancies in financial strains, social capital, and interpersonal stressors-and the degree to which variation across these measures mediates status differences in medical school outlook-are also explored.

A Maldiisotopic Approach to Discover Natural Products: Cryptomaldamide, a Hybrid Tripeptide from the Marine Cyanobacterium Moorea producens.

Genome sequencing of microorganisms has revealed a greatly increased capacity for natural products biosynthesis than was previously recognized from compound isolation efforts alone. Hence, new methods are needed for the discovery and description of this hidden secondary metabolite potential. Here we show that provision of heavy nitrogen N-nitrate to marine cyanobacterial cultures followed by single-filament MALDI analysis over a period of days was highly effective in identifying a new natural product with an exceptionally high nitrogen content.

Pattern classification of EEG signals reveals perceptual and attentional states.

Pattern classification techniques have been widely used to differentiate neural activity associated with different perceptual, attentional, or other cognitive states, often using fMRI, but more recently with EEG as well. Although these methods have identified EEG patterns (i.e.

High genetic diversities between isolates of the fish parasite Cryptocaryon irritans (Ciliophora) suggest multiple cryptic species.

The ciliate protozoan Cryptocaryon irritans parasitizes marine fish and causes lethal white spot disease. Sporadic infections as well as large-scale outbreaks have been reported globally and the parasite's broad host range poses particular threat to the aquaculture and ornamental fish markets. In order to better understand C.

Later adults' cultural life scripts of middle and later adulthood.

The cultural life script (CLS) refers to expected prototypical life events, often including life transitions overwhelmingly occurring at ages 11-30. This study outlined CLS events at ages after the majority of these events typically occur. Participants, age 38-76, nominated events they expected a person of their age to experience in the future.

Health & access to care among working-age lower income adults in the Great Recession: Disparities across race and ethnicity and geospatial factors.

In the United States (US) and elsewhere, residents of low resource areas face health-related disparities, and may experience different outcomes throughout times of severe economic flux. We aimed to identify individual (e.g.

Climate effects on late-season flight times of Massachusetts butterflies.

Although the responses of living organisms to climate change are being widely investigated, little attention has been given to such effects late in the growing season. We studied the late-season flight times of 20 species of butterflies in a geographically limited region, the state of Massachusetts in the USA, by examining change in dates of flight over a 22-year period and in response to average monthly temperature and precipitation. By analyzing the last 10% of each year's observations reported by observers of the Massachusetts Butterfly Club, we found that seven species remain in flight significantly later into the fall than they did two decades earlier, while two species show reduced late-season flight.

Geographic Disparities in Availability of Opioid Use Disorder Treatment for Medicaid Enrollees.

Objective: To examine county-level geographic variation in treatment admissions among opioid treatment programs (OTPs) that accept Medicaid in the continental United States.

Data Sources/study Setting: Data come from the 2012 National Survey of Substance Abuse Treatment Services.

Study Design/data Collection: We used local measures of spatial autocorrelation (LISA) analysis to identify (1) clusters of counties with higher and lower than average rates of opioid use disorders and (2) clusters of counties with higher and lower than average treatment admissions among OTPs that accept Medicaid, adjusting for county population size.

In God we trust? Neural measures reveal lower social conformity among non-religious individuals.

Even in predominantly religious societies, there are substantial individual differences in religious commitment. Why is this? One possibility is that differences in social conformity (i.e.

Increased Testosterone Decreases Medial Cortical Volume and Neurogenesis in Territorial Side-Blotched Lizards ().

Variation in an animal's spatial environment can induce variation in the hippocampus, an area of the brain involved in spatial cognitive processing. Specifically, increased spatial area use is correlated with increased hippocampal attributes, such as volume and neurogenesis. In the side-blotched lizard (), males demonstrate alternative reproductive tactics and are either territorial-defending large, clearly defined spatial boundaries-or non-territorial-traversing home ranges that are smaller than the territorial males' territories.

Plasmid-mediated resistance to cephalosporins and quinolones in Escherichia coli from American crows in the USA.

American crow (Corvus brachyrhynchos) faeces were tested for Escherichia coli with plasmid-mediated quinolone resistance (PMQR), extended-spectrum beta-lactamases (ESBL) and AmpC beta-lactamases. A total of 590 faecal samples were collected at four roosting sites in the USA and cultivated on selective media. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were performed to assess clonality.

Improving undergraduate STEM education: The efficacy of discipline-based professional development.

We sought to determine whether instructional practices used by undergraduate faculty in the geosciences have shifted from traditional teacher-centered lecture toward student-engaged teaching practices and to evaluate whether the national professional development program On the Cutting Edge (hereinafter Cutting Edge) has been a contributing factor in this change. We surveyed geoscience faculty across the United States in 2004, 2009, and 2012 and asked about teaching practices as well as levels of engagement in education research, scientific research, and professional development related to teaching. We tested these self-reported survey results with direct observations of teaching using the Reformed Teaching Observation Protocol, and we conducted interviews to understand what aspects of Cutting Edge have supported change.

Diversity and Universality of Endosymbiotic in the Fish Parasite .

Although the presence of endosymbiotic rickettsial bacteria, specifically Megaira, has been reported in diverse habitats and a wide range of eukaryotic hosts, it remains unclear how broadly Megaira are distributed in a single host species. In this study we seek to address whether Megaira are present in most, if not all isolates, of the parasitic ciliate . Conserved regions of bacterial 16S rRNA genes were either PCR amplified, or assembled from deep sequencing data, from 18 isolates/populations of sampled worldwide (Brazil, Taiwan, and USA).

Plants promote mating and dispersal of the human pathogenic fungus Cryptococcus.

Infections due to Cryptococcus are a leading cause of fungal infections worldwide and are acquired as a result of environmental exposure to desiccated yeast or spores. The ability of Cryptococcus to grow, mate, and produce infectious propagules in association with plants is important for the maintenance of the genetic diversity and virulence factors important for infection of animals and humans. In the Western United States and Canada, Cryptococcus has been associated with conifers and tree species other than Eucalyptus; however, to date Cryptococcus has only been studied on live Arabidopsis thaliana, Eucalyptus sp.

Parental perception and factors associated with treatment strategies for primary nocturnal enuresis.

Objective: The aim was to investigate the factors influencing parents seeking reasonable managements for their child and their overall outlook toward primary nocturnal enuresis (PNE).

Study Design: We recruited 93 children with PNE from enuresis clinics and requested their parents to complete questionnaires regarding their child's medical history and behavior, their methods for coping with PNE, and their perception of enuresis. Logistic regression models were applied to investigate factors influencing the parents to adopt a positive approach toward enuresis and to subsequently seek a medical consultation.

Dementia and co-occurring chronic conditions: a systematic literature review to identify what is known and where are the gaps in the evidence?

Objective: The challenges posed by people living with multiple chronic conditions are unique for people with dementia and other significant cognitive impairment. There have been recent calls to action to review the existing literature on co-occurring chronic conditions and dementia in order to better understand the effect of cognitive impairment on disease management, mobility, and mortality.

Methods: This systematic literature review searched PubMed databases through 2011 (updated in 2016) using key constructs of older adults, moderate-to-severe cognitive impairment (both diagnosed and undiagnosed dementia), and chronic conditions.