"Are you a homophobic racist?": Applying lay theory of generalized prejudice to the discrimination-distress link.

Objectives: The present research aimed to examine, among lesbian, gay, bisexual, transgender, and queer (LGBTQ +) people of color, whether experiencing discrimination rooted in one stigmatized identity (e.g., racial/ethnic minority identity) would be positively associated with expecting discrimination rooted in both the same (e.

Structure and registry of the silica bilayer film on Ru(0001) as viewed by LEED and DFT.

Silica bilayers are stable on various metal substrates, including Ru(0001) that is used for the present study. In a systematic attempt to elucidate the detailed structure of the silica bilayer film and its registry to the metal substrate, we performed a low energy electron diffraction (I/V-LEED) study. The experimental work is accompanied by detailed calculations on the stability, orientation and dynamic properties of the bilayer at room temperature.

Examining Racial/Ethnic Differences in Positive Childhood Experiences Among Respondents in a Southern State.

Adverse childhood experiences (ACEs) and positive childhood experiences (PCEs) may impact brain development in children and adolescents into adulthood. While prior research demonstrated racial-ethnic disparities in ACEs, less is known about racial and ethnic differences in PCEs, particularly among adults. To better understand racial and ethnic differences in positive childhood experiences, this study (1) examined the prevalence of PCEs in an adult population in South Carolina (SC), a representative southern US state and (2) examined whether PCE exposure differed across racial and ethnic groups.

In Situ Hybridization as a Method to Examine Gene Regulatory Activity In Vivo.

Transcription factor-enhancer binding events are among the most well-studied protein-DNA interactions, allowing researchers to determine mechanisms of transcriptional activation or repression during development. While large-scale ChIP-sequence datasets, together with computational predictions and chromatin accessibility data, yield information on potential transcription factor binding activities, reporter gene assays provide measurable information on whether these binding activities are functional in particular cell types during development. Here, we present a detailed protocol to examine enhancer activity in Drosophila embryos using cloning, transgenesis, and in situ hybridization.

Mutation of the Drosophila melanogaster serotonin transporter dSERT impacts sleep, courtship, and feeding behaviors.

The Serotonin Transporter (SERT) regulates extracellular serotonin levels and is the target of most current drugs used to treat depression. The mechanisms by which inhibition of SERT activity influences behavior are poorly understood. To address this question in the model organism Drosophila melanogaster, we developed new loss of function mutations in Drosophila SERT (dSERT).

Using imaginary worlds for real social benefits.

We argue that imaginary worlds gain much of their appeal because they fulfill the fundamental need of human beings to feel connected to other humans. Immersion into story worlds provides a sense of social connection to the characters and groups represented in the world. By fulfilling the need to belong, imaginary worlds provide a buffer against rejection and loneliness.

Fertilization mode differentially impacts the evolution of vertebrate sperm components.

Environmental change frequently drives morphological diversification, including at the cellular level. Transitions in the environment where fertilization occurs (i.e.

Brokering Resources during a Pandemic: Exploring How Organizations and Clinics Responded to the Needs of Immigrant Communities during COVID-19.

Though COVID-19 has had sweeping implications, many immigrant groups in the United States have been disproportionately affected. The purpose of the present study is to explore the impact of COVID-19 on immigrant communities and how local immigrant-serving organizations (ISOs) have responded during the pandemic. The authors conducted in-depth qualitative interviews with executive directors and program coordinators of 31 ISOs and health clinics in Kentucky, North Carolina, and South Carolina.

School-Based Mental Health Practitioners: A Resource Guide for Educational Leaders.

Unlabelled: School-based mental health practitioners can offer enhanced support to schools and students; yet their training, roles, and expertise vary. The roles of these professionals are often conflated, misunderstood, or marginalized in their utility throughout the school system. The purpose of this manuscript is to enhance the capacity of educational leaders to make informed hiring, contracting, and role assignment decisions that best fit school and student needs regarding school mental health services.

To see or not to see (again): Dealbreakers and dealmakers in relation to social inclusion.

In this study, we replicated what is known about the relative importance of dealbreakers (i.e., traits avoided) and dealmakers (i.

Reintroduced megaherbivores indirectly shape small-mammal responses to moonlight.

Moonlight structures activity patterns of many nocturnal species. Bright moonlight often limits the activity of nocturnal prey, but dense vegetation weakens this effect. Using 8 years of live-trapping data, we asked whether reintroduced megaherbivores (Bison bison) indirectly altered moonlight avoidance by small mammals in tallgrass prairies.

Discrepancies between children's and caregivers' child maltreatment reporting and their associations with child wellbeing.

Background: Child maltreatment reporting is critical for case investigation and service disposition. However, reporting discrepancies across informants is a challenge for child welfare services.

Methods: Using data from the Fragile Families and Child Wellbeing Study (n = 3150), the current study examined child-caregiver discrepancies in reporting the frequencies of psychological and physical maltreatment.

A Case of Fungemia Caused by Postoperative Chronic Lumbar Intervertebral Disc Infection.

Postoperative surgical site infection is one of the serious postoperative complications of spine surgery, especially fungal infections. Late-stage surgical site fungal infections often lack typical clinical symptoms and have a variable clinical presentation. In this case, the patient was a senior patient with usually only tolerable pain and discomfort, which was detected 2 years after the first surgery.

The role of physico-chemical interactions in the seasonality of toxic dinoflagellate cyst assemblages: The case of the NW Patagonian fjords system.

Harmful algal blooms (HABs) are recurrent in the NW Patagonia fjords system and their frequency has increased over the last few decades. Outbreaks of HAB species such as Alexandrium catenella, a causal agent of paralytic shellfish poisoning, and Protoceratium reticulatum, a yessotoxins producer, have raised considerable concern due to their adverse socioeconomic consequences. Monitoring programs have mainly focused on their planktonic stages, but since these species produce benthic resting cysts, the factors influencing cyst distributions are increasingly gaining recognition as potentially important to HAB recurrence in some regions.

Beyond editing, CRISPR/Cas9 for protein localization: an educational primer for use with "A dCas9-based system identifies a central role for Ctf19 in kinetochore-derived suppression of meiotic recombination".

CRISPR/Cas9 has dramatically changed how we conduct genetic research, providing a tool for precise sequence editing. However, new applications of CRISPR/Cas9 have emerged that do not involve nuclease activity. In the accompanying article "A dCas9-based system identifies a central role for Ctf19 in kinetochore-derived suppression of meiotic recombination," Kuhl et al.

Infectious disease and cognition in wild populations.

Infectious disease is linked to impaired cognition across a breadth of host taxa and cognitive abilities, potentially contributing to variation in cognitive performance within and among populations. Impaired cognitive performance can stem from direct damage by the parasite, the host immune response, or lost opportunities for learning. Moreover, cognitive impairment could be compounded by factors that simultaneously increase infection risk and impair cognition directly, such as stress and malnutrition.

Impact of stereotype threat on brain activity during memory tasks in older adults.

We report the first neuroimaging experiment to investigate the impact of explicitly activating aging stereotypes (i.e., stereotype threat) on brain activity during cognitive tasks.

Analysis of Intestinal Short-Chain Fatty Acid Metabolism Profile After Probiotics and GLP-1 Treatment for Type 2 Diabetes Mellitus.

Type 2 diabetes accounts for about 90% of diabetes patients, and the incidence of diabetes is on the rise as people's lifestyles change. Compared with GLP-1 treatment, probiotic treatment can directly regulate homeostasis of the host gut microbe, and thus homeostasis of its metabolites. Currently, the regulatory role of probiotics on intestinal metabolites after treatment of type 2 diabetes mellitus remains unclear.

Person-centered profiles of child temperament: A comparison of coder, mother, and experimenter ratings.

This study compared person-centered temperament group classifications derived from latent profile analyses on maternal reports, post-visit experimenter ratings, and task-based coder-rated behaviors at 18 months of age. Toddlers (N = 148) completed a laboratory visit with tasks intended to assess temperament and early regulatory abilities. Following the visit, experimenters rated child temperament using the Infant Behavior Record and coders rated toddlers' approach-withdrawal, affect, and regulatory behaviors across several laboratory tasks.

Plant induced defenses that promote cannibalism reduce herbivory as effectively as highly pathogenic herbivore pathogens.

Plant induced defenses may benefit plants by increasing cannibalism among insect herbivores. However, the general efficacy of plant defenses that promote cannibalism remains unclear. Using a generalist Lepidopteran herbivore (Helicoverpa zea), we examined whether plant induced defenses in Solanum lycopersicum increased cannibalism among H.

Escalating carbon emissions from North American boreal forest wildfires and the climate mitigation potential of fire management.

Wildfires in boreal forests release large quantities of greenhouse gases to the atmosphere, exacerbating climate change. Here, we characterize the magnitude of recent and projected gross and net boreal North American wildfire carbon dioxide emissions, evaluate fire management as an emissions reduction strategy, and quantify the associated costs. Our results show that wildfires in boreal North America could, by mid-century, contribute to a cumulative net source of nearly 12 gigatonnes of carbon dioxide, about 3% of remaining global carbon dioxide emissions associated with keeping temperatures within the Paris Agreement's 1.

Frequent Spindle Assembly Errors Require Structural Rearrangement to Complete Meiosis in .

The success of an organism is contingent upon its ability to faithfully pass on its genetic material. In the meiosis of many species, the process of chromosome segregation requires that bipolar spindles be formed without the aid of dedicated microtubule organizing centers, such as centrosomes. Here, we describe detailed analyses of acentrosomal spindle assembly and disassembly in time-lapse images, from live meiotic cells of .

Mesoscopic Structures and Coexisting Phases in Silica Films.

Silica films represent a unique two-dimensional film system, exhibiting both crystalline and vitreous forms. While much scientific work has focused on the atomic-scale features of this film system, mesoscale structures can play an important role for understanding confined space reactions and other applications of silica films. Here, we report on mesoscale structures in silica films grown under ultrahigh vacuum and examined with scanning tunneling microscopy (STM).