A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens.

In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV. In a screening of 114 clinical samples from four provinces in China for PEDV and TGEV, 48.

Establishment of a nanoparticle-assisted RT-PCR assay to distinguish field strains and attenuated strains of porcine epidemic diarrhea virus.

Porcine epidemic diarrhea virus (PEDV) can cause serious disease and even death in neonatal piglets, resulting in serious damage to the swine industry worldwide. Open reading frame 3 (ORF3) is the only accessory gene in the PEDV genome. Previous studies have indicated that PEDV vaccine strains have a partial deletion in ORF3.

Development and evaluation of the rVP-ELISA for detection of antibodies against porcine parvovirus.

The gene encoding the VP2 protein of porcine parvovirus (PPV) was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination (HA), Western blotting using PPV positive sera. The purified rVP2 proteins were used as coating antigen to establish a rVP-ELISA method for detection of PPV positive and negative sera from pigs.

A sensitive and specific nanoparticle-assisted PCR assay for rapid detection of porcine parvovirus.

Unlabelled: A novel nanoparticle-assisted polymerase chain reaction (nanoPCR) assay to detect porcine parvovirus (PPV) is described here. Primers for this assay were designed based on the conserved region of the nonstructural protein 1 (NS1) gene of PPV, which encodes one of the nonstructural proteins. The sensitivity of the PPV nanoPCR assay was measured by using diluted recombinant plasmids in which the PPV NS1 gene had been inserted.