Web Visualization for Spatiotemporal Genomic Epidemiology, Annotation, and Mutation Dynamics of Orthohantavirus hantanense Using Nextstrain.

The phylogeographic inference approach aims to connect genomic data with epidemiology to understand the spread and evolution of pathogens using visualization of spatiotemporal reconstructions. Orthohantavirus hantanense (HTNV), the causative agent of hemorrhagic fever with renal syndrome (HFRS), represents a significant global public health concern. Here, we introduce a localized Nextstrain platform for HTNV, offering a comprehensive resource for facilitating spatiotemporal genomic surveillance and the study of evolutionary dynamics of viral genomes.

The Social Construction of Categorical Data: Mixed Methods Approach to Assessing Data Features in Publicly Available Datasets.

Background: In data-sparse areas such as health care, computer scientists aim to leverage as much available information as possible to increase the accuracy of their machine learning models' outputs. As a standard, categorical data, such as patients' gender, socioeconomic status, or skin color, are used to train models in fusion with other data types, such as medical images and text-based medical information. However, the effects of including categorical data features for model training in such data-scarce areas are underexamined, particularly regarding models intended to serve individuals equitably in a diverse population.

The Use of Acellular Dermal Matrix May Not Increase 30-Day Complications in Direct-to-Implant Breast Reconstruction: A Multi-institutional Analysis of 10,177 Cases.

Background: Direct-to-implant (DTI) breast reconstruction offers immediate aesthetic and psychological benefits, but the role of acellular dermal matrix (ADM) remains debated. Using a multi-institutional database, this study evaluates and compares outcomes between ADM-assisted and non-ADM DTI procedures.

Methods: The American College of Surgeons National Surgical Quality Improvement Program database from 2008 to 2022 was queried to identify female patients who underwent DTI breast reconstruction for oncological purposes.

Collegiate Male Athlete Attitudes Toward Male and Female Strength and Conditioning Coaches.

Taylor, K, and Little, SJ. Collegiate male athlete attitudes toward male and female strength and conditioning coaches. J Strength Cond Res 39(2): 277-280, 2025-Sports have long been male dominated, but there have been recent increases in the involvement of women in athletic administration and athlete support, that is, strength and conditioning.

Phenomenological Description of the Experiences of Teenagers With Critically Ill Parents.

Background: Critical illness not only threatens the life of the patient but also may profoundly impact the lives of their loved ones. For teenagers with a critically ill parent, these impacts may have significant, developmentally impactful effects. A descriptive understanding of these effects may advance scholarly understanding of the challenges these teenagers face.

Hemodynamic analysis of left ventricular unloading with Impella versus IABP during VA-ECMO.

: The utilization of intra-aortic balloon pump (IABP) and Impella has been suggested as means of left ventricular unloading in veno-arterial extracorporeal membrane oxygenation (VA-ECMO) patients. This study aimed to assess the local hemodynamic alterations in VA-ECMO patients through simulation analyses. : In this study, a 0D-3D multiscale model was developed, wherein resistance conditions were employed to define the flow-pressure relationship.

Conservative Reconstruction of the Lower Limb with a Bilayer Porous Collagen Matrix after a Spider Bite.

Brown recluse spider bites may cause symptoms ranging from local cutaneous reactions to systemic visceral loxoscelism. Most bites are self-limiting, but some can lead to necrotic ulcerations with severe complications and soft tissue defects. Necrotizing ulcers are uncommon and have various clinical presentations, so no standard treatment exists.

Use of Electronic Health Records to Identify Factors Related to Skin Changes in Terminal Patients.

Objective: To investigate the incidence of skin changes at life's end (SCALE) among hospice patients and identify associated factors.

Methods: The authors conducted a retrospective chart review of demographic data, medical history, Braden Scale assessment scores, Charlson Comorbidity Index, symptom records, and medical treatments of patients admitted to a local teaching hospital's hospice unit between May 2019 and April 2021.

Results: Most (79%) of the 300 hospice patients included in the study had cancer.

Markers for Pressure Injury Risk in Individuals with Chronic Spinal Cord Injury: A Pilot Study.

Objective: To identify markers associated with pressure injury (PrI) history in individuals with spinal cord injury (SCI) using two approaches: skin blood flow (SBF) response toward localized heating, and serum marker for insulin resistance.

Methods: For this cross-sectional, observational study of adults with chronic traumatic SCI at T12 and above, researchers recruited two groups of participants: with history of PrI (group 1), and without history of PrI (group 2). The study protocol included obtaining fasting blood samples and measurement of SBF at bilateral heels with localized heating of 42 °C for 30 minutes from all participants.

Pooled prevalence and associated factors of traditional uvulectom among children in Africa: A systematic review and meta-analysis.

Background: Traditional childhood uvulectomy (TCU) is an unregulated cultural practice associated with significant health risks, including infections, anemia, aspiration, and oral or pharyngeal injuries. The reuse of unsafe tools such as blades, needles, or thread loops exacerbates the spread of infectious diseases like HIV and hepatitis B. Despite its clinical significance, the pooled prevalence and associated factors of TCU have not been adequately examined through systematic reviews or meta-analyses.

Fertility before and after treatment of the patient with Leydig cell tumor - case report.

Even though Leydig cell tumor (LCT) represents the most common neoplasia among testicular sex cord-stromal tumors (SCSTs), it is a rare condition, comprising 1-2% of all testicular tumors, with a 10% risk of malignancy most commonly located in retroperitoneal lymph nodes. LCTs may demonstrate various clinical manifestations - from asymptomatic intratesticular swelling through nonspecific symptoms such as loss of libido, impotence or infertility, up to feminizing or virilizing syndromes due to hormonal activity of the tumor. This article presents a case of Leydig cell tumor that was associated with azoospermia what have rarely been reported worldwide.

Practical Uses of Fruits in Ancient Medicine (based on the treatise of Quintus Gargilius Martialis Medicinae ex oleribus et pomis).

Objective: Aim: The aim of the article is to study the therapeutic effect and pharmacological characteristics of using fruits, berries, and nuts in ancient medicine, expanding and deepening knowledge in the history of medicine.

Patients And Methods: Materials and Methods: The study material was Medicinae ex oleribus et pomis, the work of Quintus Gargilius Martialis, a third-century Roman writer, a systematizer of rules for cultivating and medical application of over 60 types of vegetables and fruits. The methodological basis of the research is a set of general scientific and special research methods, including analysis and synthesis, induction and deduction, historical, interdisciplinary, descriptive methods, and the method of contextual analysis.

Assessing Traumatic Brain Injury in Refugees: Feasibility, Usability, and Prevalence Insights From a US-Based Clinical Sample.

Objective: This study assessed (1) the feasibility and usability of traumatic brain injury (TBI) assessment using the Ohio State University TBI Identification Method (OSU-TBI-ID) in a sample of English and Spanish-speaking refugees and asylum seekers (hereafter refugees), and (2) the prevalence and characteristics of TBI in this population.

Setting And Participants: Refugees seeking care from Massachusetts General Hospital (MGH) Asylum Clinic, the MGH Chelsea HealthCare Center, and other asylum programs in the Greater Boston Area.

Design And Main Measures: Bilingual clinical research coordinators screened 158 English and Spanish-speaking refugees using the OSU-TBI-ID.

Sex Differences in Early/Unplanned Separation Among US Service Members With a History of Mild Traumatic Brain Injury.

Objective: To investigate the incidence of early/unplanned (E/U) separations following mild traumatic brain injury (mTBI) and assess whether sex impacts the hazard of separation.

Setting: Military Health System (MHS).

Participants: Active duty service members (N = 75,730) with an initial mTBI diagnosis in military records between January 2011 and January 2018.

Mental health challenges and perceptions of stigma among youth living with HIV in Tanzania.

Youth living with HIV (YLWH) face psychosocial challenges and HIV-related stigma, which impact adherence to antiretroviral therapy (ART). This study was designed to understand better the change in mental health symptoms and experiences with stigma among YLWH in Tanzania who completed the original pilot Sauti ya Vijana (SYV), a mental health and life skills group intervention. YLWH who completed SYV and demonstrated a change of ≥2 points in either direction on their Patient Health Questionnaire PHQ-9 (depression screener) from baseline to 18 months were purposively sampled.

Milestone events in recessive dystrophic epidermolysis bullosa (RDEB): findings of the PEBLES Study.

Background: In recessive dystrophic epidermolysis bullosa (RDEB), complications like oesophageal strictures, hand contractures, cardiomyopathy and cutaneous squamous cell carcinoma (SCC) may develop, necessitating procedures such as oesophageal dilatation (OD), gastrostomy tube placement and hand surgery.

Objectives: To determine prevalence and age of onset of milestone events by RDEB subtype, specifically dysphagia, first OD, first gastrostomy tube, first hand surgery, cardiomyopathy, first SCC and death.

Methods: The Prospective Epidermolysis Bullosa Longitudinal Evaluation Study (PEBLES) is a register study of individuals with RDEB which records comprehensive EB- and non-EB-related health information.

Flight phenology and influence of region and habitat on the abundance of Xylosandrus germanus and Anisandrus maiche (Coleoptera: Curculionidae: Scolytinae) in New York.

The non-native wood-boring and symbiotic fungus-culturing Xylosandrus germanus (Blandford) was first reported in New York apple orchards in 2013. Trapping surveys have been conducted annually since to assist growers in timely applications of preventative control measures. In 2021, a similar-looking introduced species, Anisandrus maiche (Kurentsov), was identified in traps in west central New York.

Factors influencing medication adherence among hypertensive patients in primary care settings in Central Vietnam: A cross-sectional study.

Background: Medication adherence plays a crucial role in effectively managing hypertension, a significant public health concern, especially in regions like Central Vietnam. This study aimed to assess medication adherence levels among hypertensive patients in primary care settings and explore the factors influencing adherence within this specific population.

Methods: We conducted a cross-sectional study to evaluate medication adherence and its determinants among individuals with hypertension in Central Vietnam.

Incident heart failure and recurrent coronary events following acute myocardial infarction.

Background And Aims: Recurrent myocardial infarction (MI) and incident heart failure (HF) are major post-MI complications. Herein, contemporary post-MI risks for recurrent MI and HF are described.

Methods: A total of 6804 patients with a primary discharge diagnosis of MI at 28 Baylor Scott & White Health hospitals (January 2015 to December 2021) were studied.

The Developmental Origin of Novel Complex Morphological Traits in Lepidoptera.

Novel traits in the order Lepidoptera include prolegs in the abdomen of larvae, scales, and eyespot and band color patterns in the wings of adults. We review recent work that investigates the developmental origin and diversification of these four traits from a gene-regulatory network (GRN) perspective. While prolegs and eyespots appear to derive from distinct ancestral GRNs co-opted to novel body regions, scales derive from in situ modifications of a sensory bristle GRN.

The Genomics Revolution Drives a New Era in Entomology.

Thanks to the fast development of sequencing techniques and bioinformatics tools, sequencing the genome of an insect species for specific research purposes has become an increasingly popular practice. Insect genomes not only provide sets of gene sequences but also represent a change in focus from reductionism to systemic biology in the field of entomology. Using insect genomes, researchers are able to identify and study the functions of all members of a gene family, pathway, or gene network associated with a trait of interest.

Inbreeding and Outbreeding Depression in Wild and Captive Insect Populations.

Major changes in genetic variation are generally considered deleterious to populations. The massive biodiversity of insects distinguishes them from other animal groups. Insect deviant effective population sizes, alternative modes of reproduction, advantageous inbreeding, endosymbionts, and other factors translate to highly specific inbreeding and outbreeding outcomes.

Molecularly imprinted electrochemical sensor to sensitively detect tetramethylpyrazine in Baijiu.

Tetramethylpyrazine (TMP) is a compound known for its natural health benefits, but current detection methods for TMP are overly expensive and time-consuming. In this study, we developed functional materials with TMP molecular recognition properties using molecularly imprinted technology. As TMP does not produce electrochemical signals in the detection potential range, hexacyanoferrate was selected as a redox probe, combined with the highly conductive polymer PEDOT:PSS to enhance electrode conductivity.

BbGSD: Black-boned Sheep Genome SNP Database.

Lanping black-boned (LPBB) sheep are a unique and rare ruminant species, characterized by black pigmentation in the skin and internal organs. Thus far, LPBB are the only known animal with heritable melanin characteristics besides the black-boned chicken, and the only mammal known to contain a large amount of melanin in the body. LPBB have therefore attracted substantial research attention, due to their potential contribution to medicine.

Incidence of Wounds Associated with Posterior Midline Incision for Achilles Tendon Pathology in a Large Cohort at a Tertiary Care Center.

Background: We sought to determine the wound complications associated with a straight midline incision overlying the Achilles tendon. Data on sex, age, body mass index (BMI), surgery performed, date of surgery, and wound development were collected.

Methods: We retrospectively collected data on 217 patients with a mean ± SD age of 46.