28 results match your criteria: "Gunma Paz University Graduate School of Health Sciences[Affiliation]"

Objectives: This study was conducted to investigate factors involved in anaphylaxis related to diclofenac etalhyaluronate (DEH) [product name: Joyclu® (JCL)] (containing DEH and macrogol 400), which is used to treat patients with osteoarthritis.

Methods: Patients with osteoarthritis were divided into two groups that had (experienced patients) or had not experienced anaphylactic symptoms after JCL administration (non-experienced patients). Five tests performed to assess factors related to anaphylaxis consisted of a skin prick test (SPT) as the primary endpoint and the other tests including basophil activation test, allergen-specific IgE (sIgE) tests using enzyme-linked immunosorbent assay or immunochromatographic kits, and genetic study were secondary endpoints.

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Molecular Evolutionary Analyses of Gene in the Enterohemorrhagic (EHEC).

Microorganisms

September 2024

Department of Bacteriology, Graduate School of Medicine, Gunma University, Maebashi-shi 371-8511, Gunma, Japan.

Article Synopsis
  • The study focused on the molecular genetics of a specific gene by analyzing various gene subtypes using advanced bioinformatics techniques, revealing its evolutionary history.
  • A time-scaled phylogenetic analysis indicated that the common ancestor of the gene existed about 18,600 years ago, leading to two main lineages with different genetic divergences.
  • The findings suggest that certain subtypes of Stx2A proteins may generate effective antibodies in humans, and differences in virulence among strains could explain the success of Lineage 1.
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Background And Purpose: Stroke impairs a patient's ability to walk. In patients with acute stroke, a 6-min walking distance (6MWD) is recommended to assess walking function. Minimal clinically important difference (MCID) is used to determine the effectiveness of rehabilitation; however, the MCID for 6MWD has not been adequately validated.

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Background: Rehabilitation is important for patients with moderate-to-severe traumatic brain injury (TBI). However, the timing of early rehabilitation initiation is ambiguous, and its safety and effectiveness are unknown.

Objective: To examine the effectiveness and safety of early rehabilitation in patients with moderate-to-severe TBI using propensity score analysis and a large database.

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Lipid-orchestrated paracrine circuit coordinates mast cell maturation and anaphylaxis through functional interaction with fibroblasts.

Immunity

August 2024

Laboratory of Microenvironmental and Metabolic Health Sciences, Center for Disease Biology and Integrative Medicine, The University of Tokyo, Tokyo 113-8655, Japan; Lipid Metabolism Project, Tokyo Metropolitan Institute of Medical Science, Tokyo 156-8506, Japan; AMED-CREST, Japan Agency for Medical Research and Development, Tokyo 100-0004, Japan. Electronic address:

Interaction of mast cells (MCs) with fibroblasts is essential for MC maturation within tissue microenvironments, although the underlying mechanism is incompletely understood. Through a phenotypic screening of >30 mouse lines deficient in lipid-related genes, we found that deletion of the lysophosphatidic acid (LPA) receptor LPA, like that of the phospholipase PLA2G3, the prostaglandin D (PGD) synthase L-PGDS, or the PGD receptor DP1, impairs MC maturation and thereby anaphylaxis. Mechanistically, MC-secreted PLA2G3 acts on extracellular vesicles (EVs) to supply lysophospholipids, which are converted by fibroblast-derived autotaxin (ATX) to LPA.

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Predictive Value of Various Atypical Cells for the Detection of Human Papillomavirus in Cervical Smears.

Int J Mol Sci

January 2024

Department of Medical Technology, Faculty of Health Sciences, Kyorin University, 5-4-1 Shimorenjaku, Mitaka-shi 181-8621, Tokyo, Japan.

It is thought that numerous genotypes of human papillomavirus (HPV) are associated with various atypical cells, such as multinucleated cells, koilocytes, binucleated cells, parakeratotic cells, and giant cells, in the cervix. We previously showed the specificity of HPV genotypes for koilocytes and multinucleated cells. Therefore, in this study, we analyzed the association among HPV genotypes and binucleated cells, parakeratotic cells, and giant cells in Papanicolaou (Pap) smears.

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Article Synopsis
  • The study focuses on reflex cytology, particularly the role of prominent nucleoli in immature metaplastic squamous cells (IM), which may be misinterpreted in diagnoses.
  • Researchers aimed to identify human papillomaviruses (HPVs) in IM clusters found in cervical intraepithelial neoplasia 2 (CIN2) using Pap smears from 32 patients.
  • Findings showed that HPV-positive IM clusters had a significantly larger median nuclear area compared to HPV-negative clusters, suggesting that certain nuclear characteristics can indicate the presence of neoplastic cells linked to HPV.
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Primary central nervous system lymphoma (PCNSL) is a malignant neoplasm of the central nervous system that is refractory to treatment and has extremely poor prognosis. One factor hindering the development of therapeutic options for PCNSL is its molecular heterogeneity and the extreme difficulty in establishing cell lines that permit intensive research on this disease. In the present study, we developed a method to propagate PCNSL cells using a contacting transwell cell culture system involving brain vascular pericytes.

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Objective: This study was conducted to investigate the mechanisms of anaphylaxis in patients with osteoarthritis of the knee and hip after diclofenac etalhyaluronate [product name: JOYCLU® (JCL)] intra-articular injection and to determine the utility of tests to investigate the mechanism involved.

Methods: In this observational study in Japan, patients aged ≥20 years with knee or hip osteoarthritis who received JCL intra-articular injection experienced anaphylactic symptoms considered related to JCL ('experienced patients') or did not experience allergic symptoms considered related to JCL ('non-experienced patients'). Basophil activation tests (BATs), specific immunoglobulin E (IgE) antibody testing by enzyme-linked immunosorbent assays (ELISAs) or immunochromatographic kit, and genome-wide association studies (GWASs) were conducted using patient blood and saliva.

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To better understand the evolution of the SARS-CoV-2 Omicron subvariants, we performed molecular evolutionary analyses of the spike () protein gene/S protein using advanced bioinformatics technologies. First, time-scaled phylogenetic analysis estimated that a common ancestor of the Wuhan, Alpha, Beta, Delta variants, and Omicron variants/subvariants diverged in May 2020. After that, a common ancestor of the Omicron variant generated various Omicron subvariants over one year.

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The identification of pathogens associated with respiratory symptoms other than the novel coronavirus disease 2019 (COVID-19) can be challenging. However, the diagnosis of pathogens is crucial for assessing the clinical outcome of patients. We comprehensively profiled pathogens causing non-COVID-19 respiratory symptoms during the 7th prevalent period in Gunma, Japan, using deep sequencing combined with a next-generation sequencer (NGS) and advanced bioinformatics technologies.

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Article Synopsis
  • The study investigates how different types of human papillomavirus (HPV) prefer to infect specific tissues, focusing on high-risk (HR) and low-risk (LR) HPV types.
  • It was found that HR types are commonly found in cervical samples, while all LR types were exclusively located in vaginal samples, suggesting distinct infection sites for each.
  • This preference for certain tissues may play a significant role in the risk of developing cervical cancer and its progression to malignant states.
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Background: Several hip fracture clinical prediction models have been developed. We conducted this study to (i) map outcomes used in clinical prediction models for hip fracture, (ii) identify the domains and instruments of predictors, and (iii) assess the risk of bias.

Methods: We performed systematic searches of studies published from June 2002 to June 2023 in the PubMed, Cochrane Library, CINAHL, CiNii, and Ichushi databases.

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Molecular evolutionary analyses of the fusion protein gene in human respirovirus 1.

Virus Res

August 2023

Department of Health Science, Gunma Paz University Graduate School of Health Sciences, Takasaki-shi, Gunma 370-0006, Japan; Advanced Medical Science Research Center, Gunma Paz University Research Institute, Shibukawa-shi, Gunma 377-0008, Japan. Electronic address:

Few evolutionary studies of the human respiratory virus (HRV) have been conducted, but most of them have focused on HRV3. In this study, the full-length fusion (F) genes in HRV1 strains collected from various countries were subjected to time-scaled phylogenetic, genome population size, and selective pressure analyses. Antigenicity analysis was performed on the F protein.

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Despite the increasing evidence of the clinical impact of -derived cephalosporinase (PDC) sequence polymorphisms, the molecular evolution of its encoding gene, , remains elusive. To elucidate this, we performed a comprehensive evolutionary analysis of . A Bayesian Markov Chain Monte Carlo phylogenetic tree revealed that a common ancestor of diverged approximately 4660 years ago, leading to the formation of eight clonal variants (clusters A-H).

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Article Synopsis
  • The HiP4 tag system is a new protein detection and purification method, using a short epitope sequence that overlaps with the traditional 6xHis-tag.
  • A monoclonal antibody against the HiP4 tag was developed, enabling high-specificity immunoassays for various applications.
  • This system enhances tandem affinity purification and mass spectrometry analysis for studying protein interactions, successfully identifying novel proteins involved with hepatitis B virus X and TDP-43.
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Article Synopsis
  • The exact causes of asthma are not fully understood, but respiratory infections, particularly those caused by rhinovirus (RV), may trigger or worsen the condition.
  • Understanding the connection between viral infections and asthma can help address differences in immune responses between viral infections and allergies.
  • The complexity of RV-induced asthma involves both the immune response to the virus and allergic reactions triggered by various cytokines, highlighting the need to explore both RV infections and host defense mechanisms for a clearer understanding.
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Molecular Evolution of the DNA Gyrase Gene.

Microorganisms

August 2022

Infectious Disease Surveillance Center, National Institute of Infectious Diseases, Musashimurayama 162-8640, Tokyo, Japan.

Article Synopsis
  • DNA gyrase, specifically the GyrA protein encoded by the
  • gene
  • , is vital for DNA replication in bacteria and mutations in GyrA lead to resistance against quinolone antibiotics like ciprofloxacin.
  • A study used advanced bioinformatics to analyze the evolution of the
  • gene
  • , revealing a common ancestor over 760 years ago and that certain mutations (T83I and D87N) related to drug resistance became prevalent after clinical use of quinolones.
  • The research showed that these mutations reduce the effectiveness of ciprofloxacin, suggesting that the
  • gene
  • evolved to help bacteria survive antibiotic treatment post-1962 when quinolone use began.
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Human papillomavirus (HPV) testing using self-collected vaginal specimens is the preferred choice to increase screening uptake. Although the HPV testing results of these samples depend on the cells that naturally exfoliate from the cervical lesion and drift into the vagina, the mechanism of when and how these exfoliated cells mix with the self-collected sample remains unclear. Hence, the study aimed to clarify the relationship between the vaginal drift of HPV-infected cells exfoliated from the cervix, and the menstrual cycle.

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Profiles of Human Papillomavirus Detection of the Multinucleated Cells in Cervical Smears.

Microorganisms

July 2021

Department of Medical Technology, Faculty of Health Sciences, Kyorin University, 5-4-1 Shimorenjaku, Mitaka-shi, Tokyo 181-8621, Japan.

Article Synopsis
  • * Using a microdissection method, HPV genotypes were identified in 651 cases, revealing specific types like HPV16, 34, and 56 linked to MNC occurrence.
  • * Findings suggest that high-risk HPV genotypes, especially HPV16 and 56, may contribute to abnormal cell changes and that the presence of MNCs could indicate potential squamous intraepithelial lesions.
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Article Synopsis
  • Dissemination of ESC-resistant Salmonella in egg production poses a public health risk, as these bacteria can acquire resistance genes through insertion sequences.
  • A study analyzed 224 Salmonella isolates across various breeding and processing facilities in Japan, uncovering that 17.9% were resistant to multiple antibiotics, but not to azithromycin and meropenem.
  • Genetic analysis revealed that certain resistant strains harbored IncA/C plasmids with resistance genes, facilitating the transfer of these genes within the breeding chain.
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Protein tyrosine phosphatase Shp2 positively regulates cold stress-induced tyrosine phosphorylation of SIRPα in neurons.

Biochem Biophys Res Commun

September 2021

Department of Laboratory Sciences, Gunma University Graduate School of Health Sciences, 3-39-22 Showa-Machi, Maebashi, Gunma, 371-8514, Japan. Electronic address:

The membrane protein SIRPα is a cold stress-responsive signaling molecule in neurons. Cold stress directly induces tyrosine phosphorylation of SIRPα in its cytoplasmic region, and phosphorylated SIRPα is involved in regulating experience-dependent behavioral changes in mice. Here, we examined the mechanism of cold stress-induced SIRPα phosphorylation in vitro and in vivo.

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To predict the clinical outcome of coronavirus disease-2019 (COVID-19), we examined relationships among epidemiological data, viral load, and disease severity. We examined viral loads of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) in fatal (15 cases), symptomatic/survived (133 cases), and asymptomatic cases (138 cases) using reverse transcription quantitative real-time PCR (RT-qPCR). We examined 5768 nasopharyngeal swabs (NPS) and attempted to detect the SARS-CoV-2 genome using RT-qPCR.

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Immunoglobulin A (IgA)-albumin complexes may be associated with pathophysiology of multiple myeloma, although the etiology is not clear. Detailed structural analyses of these protein-protein complexes may contribute to our understanding of the pathophysiology of this disease. We analyzed the structure of the IgA-albumin complex using various electrophoresis, mass spectrometry, and in silico techniques.

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