A novel electrochemical sensing platform based on the esterase extracted from kidney bean for high-sensitivity determination of organophosphorus pesticides.

Similar to acetylcholinesterase, the activity of plant-derived esterase can also be inhibited by organophosphorus pesticides. Therefore, an electrochemical sensing platform using kidney bean esterase as a new detection enzyme was proposed for the highly sensitive determination of organophosphorus pesticides. Purified kidney bean esterase was obtained by an efficient and economical aqueous two-phase extraction method.