Genome-Wide Genomic Analysis and Evolutionary Insights into Bovine Coronavirus Strains in Southwest China.

The global epidemic of bovine coronavirus (BCoV) has caused enormous economic losses. The characterisation and genetic composition of endemic strains in Southwest China remain elusive. This study aimed to fill this gap by isolating three BCoV strains from this region and sequencing their whole genomes.

Fatty acid binding protein 1 and fatty acid synthetase over-expression have differential effects on collagen III synthesis and cross-linking in Zongdihua pig primary adipocytes.

The purpose of this study was to determine whether FABP1 and FAS regulate expression of collagen and its crosslinking via lysyl oxidase in isolated adipocytes from Zongdihua pigs. We aimed to identify biochemical processes affecting meat quality using molecular tools to provide a basis for breeding improvement of these animals. We measured expression levels of FABP1 and related genes using qRT-PCR in longissimus dorsi muscle and subcutaneous adipose tissues.

Protective effects of Fagopyrum dibotrys on oxidized oil-induced oxidative stress, intestinal barrier impairment, and altered cecal microbiota in broiler chickens.

The objective of this study was to evaluate protective effects of Fagopyrum dibotrys on antioxidant ability, intestinal barrier functions, and cecal microbiota in broiler chickens fed oxidized soybean oil. A total of 640 male Tiejiaoma broilers were randomly assigned to 8 treatments with 8 cages (10 birds per cage), as follows: birds fed basal diets containing fresh soybean oil and 0, 0.5, 1, or 2% F.

Genome-wide analysis of mRNAs, lncRNAs, and circRNAs during intramuscular adipogenesis in Chinese Guizhou Congjiang pigs.

Intramuscular fat content is an important determinant of meat quality, and preadipocyte differentiation plays a critical role in intramuscular fat deposition in pigs. However, many types of RNA differentiation, including messenger RNA (mRNA), long non-coding RNA (lncRNA), and circular RNA (circRNA) remain unreported despite their crucial roles in regulating adipogenesis. Chinese Guizhou Congjiang pigs are raised in the Guizhou province of China for their high-quality meat.

Severe pantothenic acid deficiency induces alterations in the intestinal mucosal proteome of starter Pekin ducks.

Background: Pantothenic acid deficiency (PAD) results in growth depression and intestinal hypofunction of animals. However, the underlying molecular mechanisms remain to be elucidated. Mucosal proteome might reflect dietary influences on physiological processes.

Cloning of PCS gene (TpPCS1) from Tagetes patula L. and expression analysis under cadmium stress.

Phytochelatins (PCs) constitute an important mechanism for plants to resist heavy metal stress. Widely found in higher plants, they are small heavy metal binding peptides, synthesized through catalysis of phytochelatin synthase (PCS). We speculate that there may be PCS genes in Peacock grass (Tagetes patula L.

Prevalence of fluoroquinolone resistance and mutations in the gyrA, parC and parE genes of Riemerella anatipestifer isolated from ducks in China.

Background: Riemerella anatipestifer is one of the most serious infectious disease-causing pathogens in the duck industry. Drug administration is an important method for prevention and treatment of infection in duck production, leading to widespread drug resistance in R. anatipestifer.

VEGF/Flk1 Mechanism is Involved in Roxarsone Promotion of Rat Endothelial Cell Growth and B16F10 Xenograft Tumor Angiogenesis.

The potential angiogenic effect of roxarsone, a feed additive widely used to promote animal growth worldwide, was demonstrated recently. We explored the mechanism of vascular endothelial growth factor (VEGF) and its receptor (VEGFR) in roxarsone promotion of rat vascular endothelial cells (ECs) and B16F10 mouse xenografts. ECs were treated with 0.

Pan-genome analysis of Riemerella anatipestifer reveals its genomic diversity and acquired antibiotic resistance associated with genomic islands.

Riemerella anatipestifer is a gram-negative bacterium that leads to severe contagious septicemia in ducks, turkeys, chickens, and wild waterfowl. Here, a pan-genome with 32 R. anatipestifer genomes is re-established, and the mathematical model is calculated to evaluate the expansion of R.

Rifampin resistance and its fitness cost in Riemerella anatipestifer.

Background: Riemerella anatipestifer (R. anatipestifer) is one of the most important poultry pathogens worldwide, with associated infections causing significant economic losses. Rifampin Resistance is an important mechanism of drug resistance.

First Report of Integrative Conjugative Elements in Isolates From Ducks in China.

We report for the first time the occurrence of integrative conjugative elements (ICEs) in () isolated from diseased ducks in China. For this purpose, a total of 48 genome sequences were investigated, which comprised 30 publicly available genome sequences, and 18 clinical isolates genomes sequences. Two ICEs, named ICERCAD0133-1 and ICERCAD0179-1 following the classic nomenclature system, were identified in through the use of bioinformatics tools.

Effects of post-hatch brooding temperature on performance of starter and growing Pekin ducks.

An experiment was conducted to investigate the effects of post-hatch brooding temperature on the growth performance of starter Pekin ducks from hatch to 14 D of age and the subsequent growth performance and carcass trait of growing ducks from 15 to 42 D of age. A total of 360 one-day-old male White Pekin ducks were allocated randomly to 6 environment-controlled chambers with ambient temperature set at 26, 28, 30, 32, 34, and 36°C from hatch to 7 D of age, respectively, and then decreased gradually to 26°C at 14 D of age. At 14 D of age, all ducks from all chambers were transferred to a duck barn with ambient temperature maintaining from 26 to 22°C and these birds continued to be raised from 14 to 42 D of age.

Effects of vitamin A on growth performance and tissue retinol of starter White Pekin ducks.

A dose-response experiment with 8 supplemental vitamin A levels (0, 500, 1,000, 1,500, 2,500, 3,500, 7,000, and 14,000 IU/kg) were conducted to examine the effects of vitamin A on growth performance and tissue retinol of starter White Pekin ducks. A total of 512 one-day-old male Pekin ducks were randomly divided into 8 treatments and each treatment contained 8 replicate pens of 8 ducks. All these ducks were reared in raised wire-floor pens from hatch to 21 D of age.

Molecular cloning, structural analysis, and tissue expression of the TNNT3 gene in Guizhou black goat.

The vertebrate fast skeletal troponin T (TNNT3) protein is an important regulatory and structural component of thin filaments in skeletal muscle, which improves meat quality traits of livestock and poultry. In this study, the troponin T isoforms from adult goat (skeletal muscle mRNA) were identified. We isolated the full-length coding sequence of the goat TNNT3 gene (GenBank: KM042888), analyzed its structure, and investigated its expression in different tissues from different aged goats (10, 30, 90, 180, and 360 days old).