Expert consensus for diagnosis and treatment using medical thoracoscopy in China.

Medical thoracoscopy is a commonly used endoscopic technique for the diagnosis and treatment of respiratory diseases. As an invasive technique, it is mainly used for pleural effusions and pleural diseases that cannot be diagnosed by non-invasive methods. It is also of great application in the diagnosis and treatment of certain other diseases.

[Outcome of the kidney transplant recipients with chronic hepatitis B virus].

Objective: To investigate the outcome of the kidney transplant recipients with different grades and stages of chronic hepatitis B virus after receiving renal transplantation for 3 years.

Methods: Thirty nine cases of kidney transplant recipients with hepatitis B virus and 20 cases of kidney transplant recipients (control group) between August 2000 and February 2002 were studied. Before the transplantation, the patients were divided into 4 groups: the mild hepatitis group (Group A, n = 8), the middle hepatitis group (Group B, n = 6), the severe hepatitis group (Group C, n =5) according to pathological diagnosis by percutaneous liver biopsy, and the control group (Group D).

[Modified abdominal radical trachelectomy for treatment of cervical carcinomas].

Objective: To investigate the possibility and effect of modified abdominal radical trachelectomy in treating women with early cervical cancers who desired to preserve fertility.

Methods: From October 2002 through September 2004, we performed abdominal radical trachelectomy with a modified Smith's style on 13 patients which were biopsy-proven early stage cervical carcinomas and had a desire to preserve fertility. Pelvic lymphadenectomies were performed in all patients.

[Establishment of a neonatal piglet model of multiple organ dysfunction syndrome].

Objective: To study the reliability of establishing a neonatal piglet model of multiple organ dysfunction syndrome (MODS) by cecal ligation and puncture (CLP).

Methods: Fourteen neonatal piglets were randomly assigned into Experiment group (n=9) and Control group (n=5). MODS model was established in the piglets from the Experiment group by CLP.

Prenatal molecular diagnosis of Duchenne and Becker muscular dystrophy.

Objective: Duchenne and Becker muscular dystrophy (DMD/BMD) is an X-linked lethal recessive disease caused by mutations in the dystrophy gene. There is no efficient treatment for this serious and disabling disease. We established a combination method to detect carriers and perform prenatal diagnosis.

[The establishment and clinical application of multicolor fluorescence in situ hybridization].

Objective: To establish a rapid and convenient method of multi-color fluorescence in situ hybridization (FISH) on several different tissue samples: peripheral blood samples, amniotic fluid, embryos and bone marrow samples.

Methods: FISH analysis was carried out on different tissue samples, using probes specific for chromosomes 13,18,21,X and Y or for BCR/ABL gene.

Results: FISH analysis could reveal hybridization signals on metaphase chromosomes and interphase nuclei, it is also possible to detect chromosome aberrations.

[Research on Wang Mengying's theory of nourishing - Yin and protecting - fluid].

As a famous physician of Zhejiang and Jiangsu in the late Qing dynasty, Wang Mengying was especially conversant with Wen Bing (Warm Disease). By reading Wang's works and his 16 - volume clinical case records now extant, it can be held that the clinical career of Wang was penetrated with the idea of nourishing - yin and protecting - fluid, which can be summarized into as. plain nourishing, reducing yang to nourish yin, activating Qi and distributing fluid, and protection prior to having been injured.

[Study on the relation between the angiotensin converting enzyme gene and pregnancy induced hypertension].

Objective: To study the relationship between the 16th intron insertion/deletion (I/D) polymorphism of angiotensin converting enzyme (ACE) gene and pregnancy induced hypertension (PIH).

Methods: The 16th intron I/D polymorphism of ACE gene in 60 PIH women (PIH group) and 50 normal pregnant women (control group) was detected by PCR. The type of ACE gene in PIH group was assessed and the serum concentration of angiotensin II (AngII) was compared between PIH group and control group.

[Correction of occipito-posterior position by maternal posture during the process of labor].

Objective: To investigate the effect on correction of occipito-posterior (OP) presentation by changing maternal posture during labor.

Method: A prospective study was conducted in 120 pregnant women from March 1994 to December 1995. Women in labor were instructed to take the lateral posture so that the resultant force of the gravity of the fetus, the buoyancy of amniotic fluid, and the intermittent uterine contraction may change the fetal position from occipito-posterior into occipito-anterior (OA) presentation.

[Glaucomatous hemodynamic changes in central retinal artery and ocular artery].

The hemodynamics of ocular artery (OA) and central retinal artery (CRA) in 100 eyes of 50 normal subjects and 61 eyes of 39 patients with glaucoma were studied with colour doppler image technique. The results showed the hemodynamic characteristics about normal and glaucomatous eyes and that the ischemic change of CRA was one of the high risk factors in glaucomatous visual functional damages. We propose a new classification of glaucomatous hemodynamics and the clinical application of hemodynamic parameters in glaucoma diagnosis.