Prediction and analysis of weighted genes in hepatocellular carcinoma using bioinformatics analysis.

The aim of the present study was to identify the differentially expressed genes (DEGs) between primary tumor tissue and adjacent non‑tumor tissue of hepatocellular carcinoma (HCC) samples in order to investigate the mechanisms of HCC. The microarray data of the datasets GSE76427, GSE84005 and GSE57957 were downloaded from the Gene Expression Omnibus database. DEGs were identified using the limma package in the R programming language.

Expression of Allograft Inflammatory Factor-1 (AIF-1) in Hepatocellular Carcinoma.

BACKGROUND Allograft inflammatory factor-1 (AIF-1) is a cytoplasmic protein cloned from activated macrophages in human and rat allografts. AIF-1 has been identified as a modulator of inflammatory response, and recently published studies have shown its increased expression in carcinogenesis. However, there are still limited data on the potential functional role of AIF-1 in hepatocellular carcinoma (HCC).

The Society for Translational Medicine: clinical practice guidelines for mechanical ventilation management for patients undergoing lobectomy.

Patients undergoing lobectomy are at significantly increased risk of lung injury. One-lung ventilation is the most commonly used technique to maintain ventilation and oxygenation during the operation. It is a challenge to choose an appropriate mechanical ventilation strategy to minimize the lung injury and other adverse clinical outcomes.

Advanced oxidation protein products induce S-phase arrest of hepatocytes via the ROS-dependent, β-catenin-CDK2-mediated pathway.

Liver regeneration has important clinical importance in the setting of partial hepatectomy (PH). Following PH, quiescent hepatocytes can reenter cell cycle to restore liver mass. Hepatocyte cell cycle progression, as the basic motivations of liver regeneration, can be disrupted by multiple pathological factors such as oxidative stress.

A rare and easily misdiagnosed tumor of the urinary bladder: primary composite pheochromocytoma.

Background: Composite pheochromocytoma, which is a tumor composed of ordinary pheochromocytoma and other components, is extremely rare in bladder. We present a case of this rarely seen tumor in bladder, and discuss the clinical features, behavior, pathologic findings, essentials of diagnosis and prognosis of this tumor after literature review.

Methods: Specimens from a 55-year-old woman with primary composite pheochromocytoma in bladder were analyzed by immunohistochemical (IHC) and fluorescence in situ hybridization (FISH).

Laparoscopic resections of colorectal cancer and synchronous liver metastases: a case controlled study.

Purpose: To investigate the efficacy and safety of laparoscopic simultaneous resections of colorectal cancer and synchronous colorectal liver metastases (SCRLM), relative to open surgery.

Methods: Between 1 January 2009 and 20 April 2014, 20 of 25 patients who underwent laparoscopic simultaneous colorectal cancer and SCRLM resections were matched with 20 of 29 patients who underwent an open approach, based on prognostic propensity scores. Perioperative results and survival outcomes were compared.

Nonrigid registration with corresponding points constraint for automatic segmentation of cardiac DSCT images.

Background: Dual-source computed tomography (DSCT) is a very effective way for diagnosis and treatment of heart disease. The quantitative information of spatiotemporal DSCT images can be important for the evaluation of cardiac function. To avoid the shortcoming of manual delineation, it is imperative to develop an automatic segmentation technique for 4D cardiac images.

[Effect of three-dimensional printing navigation templates assisting reconstruction with personalized unrestricted total knee prosthesis for treating maliganant bone tumors around knees].

Objective: ?To evaluate the effectiveness of three-dimensional (3D) printing navigation templates assisting reconstruction with personalized unrestricted total knee prosthesis to treat maliganant bone tumors around the knee.

Methods: ?Between March 2007 and September 2014, 43 patients with malignant tumor around the knee were divided into 2 groups: 3D printing navigation templates assisting reconstruction with personalized unrestricted total knee prosthesis were used in 21 cases (trial group), and conventional tumor resection and reconstruction with hinged total knee prosthesis were used in 22 cases (control group). There was no significant difference in age, gender, tumor location, pathological diagnosis, tumor stage, diameter of tumor, disease duration, and preoperative visual analogue scale (VAS) score between 2 groups (P>0.

Improved transcatheter aortic valve implantation for aortic regurgitation using a snare loop-assisted device: the first preclinical experience.

Background: Transcatheter aortic valve implantation (TAVI) is an effective percutaneous treatment for high-risk patients with severe symptomatic aortic stenosis. However, TAVI is rendered less effective in patients with noncalcified aortic valve disease because noncalcified aortic valves lack an anchor site for the implanted stent, causing the stent to relocate to an unexpected position. In this study, we developed an improved TAVI with a snare loop-assisted device, and evaluated the feasibility and safety of this strategy in noncalcified aortic valve disease.

Nonrigid Registration Regularized by Shape Information: Application to Atlas Construction of Cardiac CT Images.

Cardiac atlases play an important role in the computer-aided diagnosis of cardiovascular diseases, in particular they need to deal with large and highly variable image datasets. In this paper, we propose a new nonrigid registration algorithm incorporating shape information, to produce comprehensive atlases. For one thing, the multiscale gradient orientation features of images are combined to form the construction of multifeature mutual information.

Targeting miR-21 sensitizes Ph+ ALL Sup-b15 cells to imatinib-induced apoptosis through upregulation of PTEN.

Philadelphia chromosome positive (Ph+) acute lymphoblastic leukemia (ALL) cells are insensitive to BCR-ABL tyrosine kinase inhibitor imatinib, the underlying mechanisms remain largely unknown. Here, we showed that imatinib treatment induced significant upregulation of miR-21 and downregulation of PTEN in Ph+ ALL cell line Sup-b15. Transient inhibition of miR-21 resulted in increased apoptosis, PTEN upregulation and AKT dephosphorylation, whereas ectopic overexpression of miR-21 further conferred imatinib resistance.

Valproic acid attenuates the suppression of acetyl histone H3 and CREB activity in an inducible cell model of Machado-Joseph disease.

Machado-Joseph disease (MJD) is caused by a (CAG)n trinucleotide repeat expansion that is translated into an abnormally long polyglutamine tract. This disease is considered the most common form of spinocerebellar ataxia (SCA). In the present study, we developed stable inducible cell lines (PC12Tet-On-Ataxin-3-Q28/84) expressing ataxin-3 with either normal or abnormal CAG repeats under doxycycline control.

Stability of tibial defect reconstruction with fibular graft and unilateral external fixation: a finite element study.

Tibial defect is generally caused by high-energy injury, tumor, osteomyelitis, development deformity and bone non-union after internal fixation. This study was to determine stability of tibial defect reconstruction with fibular graft (FG) of different lengths by single free vascularized fibular graft (SFVFG) and double-barrel free vascularized fibular graft (DBFVFG). The left lower extremity of a male volunteer was scanned with computer tomography scanner.

Syphilitic bone destruction on FDG PET/CT.

A 35-year-old man underwent PET/CT scan to evaluate suspected osseous metastases from a presumed tumor of unknown origin. PET images demonstrated multiple foci of increased FDG uptake in all extremities, corresponding to the osseous destruction on the concurrent CT images. However, no primary tumor was identified.

[Signal pathway in apoptosis of K562 cells induced by STI571].

This study was aimed to investigate the effect of tyrosine kinase inhibitor (STI571) on growth and proliferation of K562 cells by using microarray method, the changes of gene expression in the process of K562 cell apoptosis induced by STI571 and the mechanism of K562 cell apoptosis. The gene microarray probes were prepared by RD-PCR technique, then the microarray of gene expression map was constructed; the morphologic changes of K562 cells were observed under phase-contrast microscopy before and after treatment with STI571; the apoptosis of K562 cells treated with STI571 was assayed by MTT method; the expression level of genes was analyzed by self-made microarray. The results indicated that after the treatment of STI571 for 24 hours, in K562 cells appeared major morphological changes, which included nuclear shrinkage, membrane bleb and scattered apoptotic bodies.

[Morbidity regularity of severe complications of hypertensive disorder complicating pregnancy in clinics].

Objective: To analyse incidence of the severe complications of hypertensive disorder complicating pregnancy and the influence on the outcome of pregnancy.

Methods: A retrospective study of 4107 cases among 71 020 cases who delivered in hospitals from 1995 to 2004 in Guangzhou was conducted.

Results: The morbidity of hypertensive disorder complicating pregnancy was 5.

[Preparation and purification of polyclonal antibody against chymopapain].

Objective: To prepare and purify polyclonal antibody against chymopapain, and to make a foundation for establishing an immunossay for chymopapain.

Methods: New Zealand rabbit was immunized with chymopapain. Antiserum was purified by Protein A and analyzed by ELISA.

Structural changes of neurons in hippocampus from infantile rats exposed to hyperbaric oxygen.

OBJECTIVE: To investigate if hyperbaric oxygen ( HBO) may induce structural changes of neurons in hippocampus from infantile rats and if the changes are reversible. METHODS: All 27 healthy SD infantile rats were exposed to HBO (0.25 MPa) or hyperbaric air (HBA) for 1 to 3 courses (10 days as 1 course).