[Effect of sodium salicylate on the auditory brain stem response threshold and expression of glutamic acid decarboxylase in spiral ganglion of juvenile and adult guinea pigs].

Objective: To study the differences of regulation of sodium salicylate on the auditory brain stem response (ABR) threshold and expression of glutamic acid decarboxylase (GAD) protein in spiral ganglion of juvenile and adult guinea pigs.

Methods: Fourty juvenile guinea pigs which were born just four days and fourty adult guinea pigs which were born thirty days were selected. They were divided four groups (group A; group B; group C; group D).

[The expression and significance of HIF-1alpha and COX-2 in laryngeal squamous cell carcinoma].

Objective: To investigate the protein and mRNA expression of HIF-1alpha and COX-2 in laryngeal squamous carcinoma, and their correlation with clinicopathologic parameters.

Method: The protein and mRNA expression of HIF-1alpha and COX-2 in fresh laryngeal squamous carcinoma tissues and adjacent normal tissues from 76 patients were examined by immunohistochemistry and reverse transcription polymerase chain reaction.

Result: 1) The protein positive expression rate of HIF-1alpha and COX-2 in tumor samples were significantly higher than that in normal tissues (P < 0.

[Effects of sodium salicylate on the expressions of gamma-aminobutyricacid and glutamate and auditory response properties of the inferior colliculus neurons].

The effects of sodium salicylate (NaSA) on the expressions of gamma-aminobutyricacid (GABA) and glutamate (Glu), and auditory response properties of the inferior colliculus neurons in mice were studied. Thirty-six Kunming mice were divided into three groups: control group (saline injection); NaSA group (NaSA 450 mg/kg, i.p.

[The effects of sodium salicylate on the expression of GABAalpha, NR1 and hearing response properties of inferior colliculus neurons in mice].

Aim: To study the effects of sodium salicylate on the expression of GABAalpha NR1 and hearing response properties of inferior colliculus neurons in mice.

Methods: Thirty-six kunming mice were divided into three groups (A, B, C,). The expression of GABAalpha NR1 were measured by using RT-PCR.

[Detection of the activity of MMP2 and MMP9 in middle ear cholesteatoma].

Objective: To study the relationship between the activity of MMP2, and bone resorption in middle ear cholesteatoma.

Method: Specimens from 41 cases of middle ear cholesteatoma and 20 cases of external auditory canal skin were analysed for molecular corresponding to MMP2 and MMP9 by using Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (SDS-PAGE) Zymography.

Result: The active levels of MMP2 and MMP9 in the cholesteatoma, which were (0.

[Expression of PTKs CDK4 and p15 in the middle ear cholesteatomatous epithelium].

Objective: To explore the cells proliferation and its molecular regulating mechanisms of cholesteatomatous epithelium from the aspect of protein tyrosine kinases (PTKs) signal transduction and cell cycle control.

Method: The expressions of phosphated PTKs, CDK4 and p15 were investigated by immunohistochemical S-P method and computer image analysis in 30 specimens of the middle ear cholesteatomatous epithelium and 19 specimens of external auditory canal epithelium from patients with chronic otitis media with cholesteatoma. The expressive results of phosphated PTKs, CDK4 and p15 were determined in same epithelium of different slices of same specimen.