[Evaluation of Chai Shao Liu Jun Tang for the treatment of chronic hepatitis B].

Objective: To evaluate the effect of Chai Shao Liu Jun Tang in combination with Lamivudine for the treatment chronic hepatitis B (CHB) patients.

Methods: 405 CHB patients in Guangdong Provincial Hospital were randomly divided into 2 groups, 220 in the treated group, and 185 in the control group. The control group was treated with Lamivudine for 18 months.

[Effect of modified xiaoyao powder for improving sleep in patients with psychological stress insomnia].

Objective: To subjectively and objectively assess the effect of Jiawei Xiaoyao Powder (JXYP) on sleep in patients with psychological stress insomnia. METHHODS: A randomized controlled study was conducted in 33 patients with psychological stress insomnia. They were assigned to 4 groups, 4 in the TCM group treated with JXYP, 5 in the Western medicine (WM) group treated with Estazolam, 9 in the integrated medicine (IM) group treated with JXYP plus Estazolam, and 10 in the control group treated with placebo.

[Logistic regression analysis on therapeutic effect of acupuncture on neck pain caused by cervical spondylosis and factors influencing therapeutic effect].

Objective: To explore the main factors influencing the therapeutic effect of acupuncture on neck pain caused by cervical spondylosis, so as to provide references for further increasing the therapeutic effect of acupuncture.

Methods: One hundred and six cases were randomly divided into an observation group and a control group, 53 cases in each group. The observation group was treated with routine acupuncture at Dazhui (GV 14), Jingbailao (Ex-HN 15) and Jianzhongshu (SI 15); and the control group was treated with sham acupuncture at 1 cm lateral to Bailao (Ex-HN 15) and Jianzhongshu (SI 15).

Application of a liquid chromatography/tandem mass spectrometry method for the pharmacokinetic study of dihydroartemisinin injectable powder in healthy Chinese subjects.

A simple, rapid and accurate liquid chromatography-electrospray ionization-tandem mass spectrometry method was developed and validated for quantification of dihydroartemisinin (DHA) in human plasma. Following a simple single-step liquid-liquid extraction with ethyl acetate, the analyte was separated on a C(18) column by isocratic elution with methanol-water-10mM ammonium acetate (80:10:10, v/v/v), and analyzed by mass spectrometry in the positive ion MRM mode. Good linearity was achieved over a wide range of 1.

[Regulatory effect of procyanidins on the expressions of interferon-gamma, interleukin-12 and transcription factor T-bet mRNA in peripheral blood mononuclear cell of patients with alopecia areata].

Objective: To investigate the regulatory effect of procyanidins (PC) on the expressions of Th1 type cytokines (IFN-gamma and IL-12) and the transcription factor T-bet mRNA in peripheral blood mononuclear cell (PBMC) in patients with alopecia areata (AA).

Methods: Above-mentioned expressions were detected using RT-PCR technique in 20 AA patients (8 of mild and 12 of severe degree) and 10 healthy subjects after phytoaemagglutinin (PHA) or PHA + PC stimulation.

Results: After being stimulated by PHA and PHA + PC, in patients with severe AA, the expression of T-bet mRNA was 0.

[Analysis of prescription for special disease/syndrome--compatibility of absorbed/metabolized components from original formula--a thinking of research].

What is the pharmacodynamic active components in Chinese medical prescription is a key problem in urgent need to solve for the development of traditional Chinese medicine (TCM). A new thinking of TCM research, "analysis of prescription for special disease/syndrome--compatibility of absorbed/metabolized components from original prescription", was proposed firstly, to analyze and seek out the components or metabolites originated from the administered prescription in blood serum of the organisms with special disease and syndrome. Secondly, to study the compatibility of these absorbed/metabolized components.

[Acupuncture for treatment of depressive neurosis: a multi-center randomized controlled study].

Objective: To observe the clinical therapeutic effect of acupuncture on depressive neurosis.

Methods: With a multi-center randomized controlled study, 440 cases were randomly divided into an acupuncture group, a prozac group, a non-acupoint needling group. In the acupuncture group, Hegu (LI 4) and Taichong (LR 3) were selected, and the Prozac group were treated with administration of 20 mg/d and the non-acupoint needling group were treated with needling the points deviating from the acupoints.

[Effects of perioperative treatment by integrated traditional Chinese and Western medicine on postoperative parameters of TURP].

Objective: To study the clinical effects of perioperative treatment by integrated traditional Chinese and Western medicine on postoperative parameters of TURP.

Methods: From Jun. 2002 to Oct.

[Effect of previous analgesia of scalp acupuncture on post-operative epidural morphine analgesia in the patient of intestinal cancer].

Objective: To probe the effect of previous analgesia of scalp acupuncture.

Methods: Sixty cases of radical operation of intestinal cancer were randomly divided into 2 groups: a scalp acupuncture plus epidural analgesia (scalp acupuncture group) and an epidural analgesia group, 30 cases in each group. The scalp acupuncture group received scalp acupuncture from 20 min before operation to the end of the operation, and epidural analgesia at the end of the operation.

[Effect of hemodialysis with Chinese herbs of supplementing qi and activating blood circulation on platelet membranous glycoprotein CD62P in patients with chronic renal failure].

Objective: To explore the effects of dialysate contained Chinese herbs for replenishing qi and activating blood circulation on platelet membranous glycoprotein CD62P in patients with chronic renal failure (CRF) undergoing hemodialysis.

Methods: Forty patients underwent maintaining hemodialysis were randomly assigned to two groups, the Western medicated group (WMG) and the Chinese herbs group (CHG). The content of CD62P in all patients was detected by ELISA before and after hemodialysis.

[Effects of scalp acupuncture on regulative ability of oxidative stress reaction at operation stage].

Objective: To study on regulative action of scalp acupuncture on oxidative stress reaction at operation stage.

Methods: Fifty two cases selected for radical operation of intestinal cancer were randomly divided into a scalp acupuncture plus general anesthesia (scalp acupuncture group) and a simple general anesthesia group (general anesthesia group), 26 cases in each group. The scalp acupuncture group were treated first with scalp acupuncture for 20 min, followed by general anesthesia, with scalp acupuncture lasted till the end of operation.

[Effect of extracted liquid from qianlietongyu on the proliferation and apoptosis on prostatic smooth muscle cells in vitro].

Objective: To explore the effect of extracted liquid from Qianlietongyu on the proliferation or apoptosis on prostatic smooth muscle cells in vitro.

Methods: After extracted liquid from Qianlietongyu treated the cultured prostatic smooth muscle cells, the anti proliferative and apoptotic indices were assessed by MTT assy and terminal deoxynucleotidyl transferase mediated dUTP nick end labeling (TUNEL) respectively.

Results: There was a significant dose-effect relationship between the concentration of extracted liquid from Qianlietongyu and the antiproliferative index on prostatic smooth muscle cells in vitro (P < 0.

[Primary exploration on immune associated genome of patients with Pi-Qi deficiency syndrome].

Objective: To investigate the abnormal change of immune function in patients with Pi-Qi deficiency Syndrome, and to explore the genomic mechanism of its genesis by cDNA chip techniques.

Methods: The cross probe was made by extracting and microamplifying the total RNA and mRNA of peripheral white blood cells (WBC) in healthy subjects and patients with chronic gastritis and ulcerative colitis, which were labeled by Cy3 and Cy5 respectively. Then equal quantity of the two labeled probes were mixed and hybridized with cDNA chip, fluorescent signal of the chips were scanned with scanner.

[Effect of tongguan capsule on coagulant and fibrinolysis system in patients with coronary heart disease after percutaneous coronary intervention].

Objective: To explore the effect and mechanism of Tongguan capsule (TGC) on coagulant and fibrinolysis system in patients with coronary heart disease (CHD) after percutaneous coronary intervention (PCI).

Methods: Adopting the prospective, randomized controlled method to observe the coagulant-fibrinolysis related indexes, including tissue plasminogen activator (t-PA), plasminogen activator inhibitor 1 (PAI-1), von Willebrand factor (vWF), antithrombin II (AT-III) and fibrinogen (FIB), in CHD patients after PCI, there were 26 patients in the treated group treated by TGC with Western medicine and 26 in the control group treated by Western medicine alone.

Results: After treatment, all the indexes were improved in both groups (P < 0.

[Study on T cell subsets and their activated molecules from the convalescent SARS patients during two follow-up surveys].

Aim: To explore the changes of peripheral blood T cell subsets and their activated molecules in convalescent SARS patients.

Methods: In two follow-up surveys of the SARS patients discharged from our hospital, the changes of CD3(+), CD4(+), CD8(+), CD8(+) CD28(+), CD8(+) CD28(-), CD3(+) CD25(+), CD3(+) CD69(+), CD3(+) HLA-DR(+)T cells and the ratio of CD4(+)/CD8(+) T cells in peripheral blood of SARS patients treated unitedly with Chinese and Western medicines were detected by flow cytometry.

Results: In two follow-up surveys, the percentages of CD3(+), CD4(+), CD8(+) CD28(+) T cells and the ratio of CD4(+)/CD8(+) T cells were remarkably lower than those of normal values(P<0.

[A study on nasal absorption of psoralen and isopsoralen in Psoralea corylifolia L].

Objective: To investigate the nasal absorption regularities of psoralen and isopsoralen of different concentrations.

Method: Building an experimental model of rat in situ nasal recirculation and determining the contents of psoralen and isopsoralen by HPLC.

Result: The nasal absorption of psoralen and isopsoralen fitted in with zero order kinetics, getting saturated with the increase of concentration.

[Study on the quality standards for xianghe suppository].

The quality standards for Xianghe suppository were studied. The TLC methods for identification of Rhizoma Coptidis, Radix Sophorae Flavescentis and Flos Caryophylli were established. A simple high performance liquid chromatographic method was established for determination of berberine and palmatine.

[Extraction technology of Fructus Psoraleae with orthogonal test].

The extraction conditions of Fructus Psoraleae were studied with orthogonal test, taking the contents of psoralen and isopsoralen as indexes and smesh degree, alcohol concentration, alcohol volume, immersion time and immersion times as factors. The results showed the optimal extraction process is pulverizing the herbs into 20 meshes powder, 6 times volume of 55% alcohol immersed three times and two days every time.