Robust self management classification via sparse representation based discriminative model for mild cognitive impairment associated with diabetes mellitus.

Diabetes Mellitus combined with Mild Cognitive Impairment (DM-MCI) is a high incidence disease among the elderly. Patients with DM-MCI have considerably higher risk of dementia, whose daily self-care and life management (i.e.

Silver-coated PMMA nanoparticles-on-a-mirror substrates as high-performance SERS sensors for detecting infinitesimal molecules.

Surface-enhanced Raman scattering (SERS) technology has attracted more and more attention due to its high sensitivity, low water interference, and quick measurement. Constructing high-performance SERS substrates with high sensitivity, uniformity and reproducibility is of great importance to put the SERS technology into practical application. In this paper, we report a simple fabrication process to construct dense silver-coated PMMA nanoparticles-on-a-mirror SRES substrates.

Prader-Willi syndrome protein necdin regulates the nucleocytoplasmic distribution and dopaminergic neuron development.

Dopamine (DA) plays important roles in various behaviors, including learning and motivation. Recently, THOC5 was identified as an important regulator in the development of dopaminergic neurons. However, how THOC5 is regulated has not been explored.

Genes involved in DMSO-mediated yield increase of entomopathogenic nematodes.

Entomopathogenic nematodes (EPNs) associated with their symbiotic bacteria can effectively kill insect pests, in agriculture, forestry and floriculture. Industrial-scale production techniques for EPNs have been established, including solid and liquid monoculture systems. It is found that supplement of 0.

Lauric-α-linolenic lipids modulate gut microbiota, preventing obesity, insulin resistance and inflammation in high-fat diet mice.

Medium- and long-chain triacylglycerols (MLCTs) are regarded as healthy premium oils; however, the health benefits of novel MLCTs enriched with lauric and α-linolenic acids are still not fully understood. This study examined the health benefits of lauric-α-linolenic structural lipids (ALSL) and physical mixture (PM) with a similar fatty acid composition in mice with obesity induced by the high-fat diet (HFD). The data indicated that ALSL is more effective than PM in counteracting obesity, insulin resistance, hyperlipidaemia, liver injury, and systemic inflammation in HFD-induced mice.

Accurate stacking engineering of MOF nanosheets as membranes for precise H sieving.

Two-dimensional (2D) metal-organic framework (MOF) nanosheet membranes hold promise for exact molecular transfer due to their structural diversity and well-defined in-plane nanochannels. However, achieving precise regulation of stacking modes between neighboring nanosheets in membrane applications and understanding its influence on separation performance remains unrevealed and challenging. Here, we propose a strategy for accurately controlling the stacking modes of MOF nanosheets via linker polarity regulation.

SARS-CoV-2 NSP3/4 control formation of replication organelle and recruitment of RNA polymerase NSP12.

β-coronavirus rearranges the host cellular membranes to form double-membrane vesicles (DMVs) via NSP3/4, which anchor replication-transcription complexes (RTCs), thereby constituting the replication organelles (ROs). However, the impact of specific domains within NSP3/4 on DMV formation and RO assembly remains largely unknown. By using cryogenic-correlated light and electron microscopy (cryo-CLEM), we discovered that the N-terminal and C-terminal domains (NTD and CTD) of SARS-CoV-2 NSP3 are essential for DMV formation.

Specific Monitoring the DNA Helicase Function via Anchor-Embedded DNA Probe.

DNA helicases play a pivotal role in maintaining genome integrity by unwinding the DNA double helix and are often considered promising targets for drug development. However, assessing specific DNA helicase activity in living cells remains challenging. Herein, the first anchor-embedded duplex (ATED) probe, 17GC, is constructed to uniquely monitor the unwinding activity of Werner syndrome helicase (WRN), a clinical anticancer target.

Patterns of Family Adversity and Bullying Involvement in Adolescents: Association With Mental Health Problems.

Introduction: Family and school environments are pivotal in adolescent development. However, the simultaneous examination of adversities in both domains remains understudied. Based on bioecological and spillover theories, this study aimed to bridge this research gap by using latent profile analysis to identify distinct patterns of family adversity and bullying involvement among Chinese adolescents.

Robust Immobilization and Activity Preservation of Enzymes in Porous Frameworks by Silica-Based "Inorganic Glue".

The development of novel methods to enhance enzyme-carrier interactions in situ, at a feasible cost, and on a large scale is crucial for improving the stability and durability of current immobilized enzyme systems used in industrial settings. Here, a pioneering approach termed "silica-based inorganic glue" is proposed, which utilizes protein-catalyzed silicification to fix enzyme within porous matrix while preserving enzyme activity. This innovative strategy offers several key benefits, including conformational stabilization of enzymes, improved interactions between enzymes and the matrix, prevention of enzyme leakage, and mitigation of pore blocking.

Linking D-Band Center Modulation with Rapid Reversible Sulfur Conversion Kinetics via Structural Engineering of VS₂.

The rapid catalytic conversion toward polysulfides is considered to be an advantageous approach to boost the reaction kinetics and inhibit the shuttle effect in lithium-sulfur (Li─S) batteries. However, the prediction of high catalytic activity Li─S catalysts has become challenging given the carelessness in the relationship between important electronic characteristics of catalysts and catalytic activity. Herein, the relationships between the D-band regulation of catalysts with reaction kinetics toward polysulfides are described.

Enhancing human ACE2 expression in mouse models to improve COVID-19 research.

Mice are one of the most common biological models for laboratory use. However, wild-type mice are not susceptible to COVID-19 infection due to the low affinity of mouse ACE2, the entry protein for SARS-CoV-2. Although mice with human ACE2 (hACE2) driven by Ace2 promoter reflect its tissue specificity, these animals exhibit low ACE2 expression, potentially limiting their fidelity in mimicking COVID-19 manifestations and their utility in viral studies.

Robust Autism Spectrum Disorder-Related Spatial Covariance Gray Matter Pattern Revealed With a Large-Scale Multi-Center Dataset.

Autism spectrum disorder (ASD) is a complex neurodevelopmental disorder and its underlying neuroanatomical mechanisms still remain unclear. The scaled subprofile model of principal component analysis (SSM-PCA) is a data-driven multivariate technique for capturing stable disease-related spatial covariance pattern. Here, SSM-PCA is innovatively applied to obtain robust ASD-related gray matter volume pattern associated with clinical symptoms.

Development and validation of the Chinese patient-centered integrated care scale.

Background: The healthcare system is currently facing challenges in transitioning to a more integrated, patient-centered care. China has implemented various practical exploration and pilot programs in developing an integrated healthcare service system. However, there remains an absence of appropriate instrument to assess integrated care from the patient's perspective, particularly one that aligns with China's national conditions.

Intensified conditioning containing decitabine versus standard myeloablative conditioning for adult patients with KMT2A-rearranged leukemia: a multicenter retrospective study.

Background: Allogeneic hematopoietic stem cell transplantation (allo-HSCT) is recommended for patients with KMT2A-rearranged (KMT2A-r) leukemia whereas relapse remains high. We aimed to determine whether intensified conditioning containing decitabine (Dec) could reduce relapse compared with standard myeloablative conditioning in adult patients with KMT2A-r leukemia.

Methods: We performed a multicenter retrospective study at seven institutions in China.

Development of an individualized dementia risk prediction model using deep learning survival analysis incorporating genetic and environmental factors.

Background: Dementia is a major public health challenge in modern society. Early detection of high-risk dementia patients and timely intervention or treatment are of significant clinical importance. Neural network survival analysis represents the most advanced technology for survival analysis to date.

Transcriptional landscape and predictive potential of long noncoding RNAs in peritoneal recurrence of gastric cancer.

Background: Long noncoding RNAs (lncRNAs) play a critical role in gastric cancer (GC) progression and metastasis. However, research comprehensively exploring tissue-derived lncRNAs for predicting peritoneal recurrence in patients with GC remains limited. This study aims to investigate the transcriptional landscape of lncRNAs in GC with peritoneal metastasis (PM) and to develop an integrated lncRNA-based score to predict peritoneal recurrence in patients with GC after radical gastrectomy.

Comparison of the results of medical approach and surgical approach of septic arthritis in native joints: a single-center retrospective study.

Background: The consequences of septic arthritis of natural joints may be devastating. The purpose of this study was to evaluate the functional results of medical approach and surgical approach for septic arthritis of native joints, and to explore whether the number of drainage tubes after arthroscopic surgery will affect the knee function of patients.

Methods: A single-center retrospective study was conducted on patients diagnosed with septic arthritis from January 2018 to January 2023.

Screening and identification of vascular endothelial cell targeting peptide in gastric cancer through novel integrated in vitro and in vivo strategy.

Purpose: Antiangiogenesis therapy has become a hot field in cancer research. Given that tumor blood vessels often express specific markers related to angiogenesis, the study of these heterogeneous molecules in different tumor vessels holds promise for advancing anti-angiogenic therapy. Previously using phage display technology, we identified a targeting peptide named GX1 homing to gastric cancer vessels for the first time.

A new distal radius fracture classification depending on the specific fragments through machine learning clustering method.

Purposes: The objective of this study was to investigate intra-articular distal radius fractures, aiming to provide a comprehensive analysis of fracture patterns and discuss the corresponding treatment strategies for each pattern.

Methods: 294 cases of intra-articular distal radius fractures lines were collected and clustered thorough K-means and hierarchical clustering algorithm. The demographic data of patients and the clinical treatment outcomes were recorded.

Higher interfragmentary compression force improves lateral tibial plateau fracture stability using locking plate fixation: experimental and simulation verification.

Background: This study investigated the impact of higher interfragmentary compression force (IFCF) on the stability of locking plate fixation in lateral tibial plateau fractures.

Methods: Biomechanical experiments and finite element analysis (FEA) were employed to compare the performance of the AO cancellous lag screw (AOCLS) and a newly developed combined cancellous lag screw (CCLS).

Results: The results demonstrated that the CCLS provided a higher IFCF without the risk of over-screwing, significantly improving fixation stability.

Combined Blockade of Lipid Uptake and Synthesis by CD36 Inhibitor and SCD1 siRNA Is Beneficial for the Treatment of Refractory Prostate Cancer.

Drug resistance is an important factor for prostate cancer (PCa) to progress into refractory PCa, and abnormal lipid metabolism usually occurs in refractory PCa, which presents great challenges for PCa therapy. Here, a cluster of differentiation 36 (CD36) inhibitor sulfosuccinimidyl oleate sodium (CD36i) and stearoyl-CoA desaturase 1 (SCD1) siRNA (siSCD1) are selected to inhibit lipid uptake and synthesis in PCa, respectively. To this end, a multiresponsive drug delivery nanosystem, HA@CD36i-TR@siSCD1 is designed.

Extraction, characterization, and hemostatic effect of collagen from the scales of Megalonibea fusca.

Marine collagen is gaining more attraction than terrestrial collagen because it is free of zoonotic disease and religious constrain. In this study, we aimed to investigate and compare the physicochemical properties and functional characteristics of acid-soluble collagen (ASC-MF) and pepsin-soluble collagen (PSC-MF) extracted from scales of Megalonibea fusca. ASC-MF and PSC-MF were evaluated in terms of yield, collagen type, amino acid composition, thermal stability, microstructure, cytotoxicity, and other physicochemical parameters.

Identification and Characterisation of Potential Targets for N6-methyladenosine (m6A) Modification during Intervertebral Disc Degeneration.

Background: The mechanism for RNA methylation during disc degeneration is unclear. The aim of this study was to identify N6-methyladenosine (m6A) markers and therapeutic targets for the prevention and treatment of intervertebral disc degeneration (IDD).

Methods: Methylated RNA immunoprecipitation sequencing (MeRIP-seq) and quantitative reverse transcription PCR (RT-qPCR) were employed to analyze m6A modifications of IDD-related gene expression.

Excess Ub-K48 Induces Neuronal Apoptosis in Alzheimer's Disease.

Background: K48-linked ubiquitin chain (Ub-K48) is a crucial ubiquitin chain implicated in protein degradation within the ubiquitin-proteasome system. However, the precise function and molecular mechanism underlying the role of Ub-K48 in the pathogenesis of Alzheimer's disease (AD) and neuronal cell abnormalities remain unclear. The objective of this study was to examine the function of K48 ubiquitination in the etiology of AD, and its associated mechanism of neuronal apoptosis.