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A reverse dot blot assay for the expanded screening of eleven Chinese G6PD mutations.
Clin Chim Acta
March 2013
Dongguan Institute of Pediatrics, Guangdong Medical Collage Affiliated Shilong Boai Hospital, 68 Xihu Third Road, Dongguan, Guangdong, China.
Background: Glucose-6-phosphate dehydrogenase (G6PD) deficiency is a multiethnic inherited disease with a particularly high prevalence in tropical and subtropical regions including southern China. A convenient and reliable method is required to detect common G6PD mutations in the Chinese population.
Methods: We developed a reverse dot blot (RDB) assay for the expanded screening of eleven mutations (c.