Rat models of postintracerebral hemorrhage pneumonia induced by nasal inoculation with or intratracheal inoculation with LPS.

Background: A stable and reproducible experimental bacterial pneumonia model postintracerebral hemorrhage (ICH) is necessary to help investigating the pathogenesis and novel treatments of Stroke-associated pneumonia (SAP).

Aim: To establish a Gram-negative bacterial pneumonia-complicating ICH rat model and an acute lung injury (ALI)-complicating ICH rat model.

Methods: We established two standardized models of post-ICH pneumonia by nasal inoculation with () or intratracheal inoculation with lipopolysaccharide (LPS).

Genomic selection strategies to overcome genotype by environment interactions in biosecurity-based aquaculture breeding programs.

Background: Family-based selective breeding programs typically employ both between-family and within-family selection in aquaculture. However, these programs may exhibit a reduced genetic gain in the presence of a genotype by environment interactions (G × E) when employing biosecurity-based breeding schemes (BS), compared to non-biosecurity-based breeding schemes (NBS). Fortunately, genomic selection shows promise in improving genetic gain by taking within-family variance into account.

Reclassification of Salisediminibacterium haloalkalitolerans Sultanpuram et al. 2015 as a Later Heterotypic Synonym of Salisediminibacterium halotolerans Jiang et al. 2012.

In the present study, the taxonomic position of Salisediminibacterium haloalkalitolerans was evaluated by determining the 16S rRNA gene sequence similarity, genome relatedness, and phylogenetic analyses. The 16S rRNA gene sequences extracted from the genomes of Salisediminibacterium haloalkalitolerans 10nlg and Salisediminibacterium halotolerans DSM 26530 showed 100% similarity, supporting their classification as the same species. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between S.

CK2α-mediated phosphorylation of DUB3 promotes YAP1 stability and oncogenic functions.

The aberrant upregulation of Yes-associated protein 1 (YAP1) in a variety of solid cancers contributes to tumor progression and poor clinical outcomes, rendering it an appealing therapeutic target. However, effective therapies to directly target YAP1 remain challenging. In this study, we perform a high-throughput screening and identify Casein kinase II (CK2) as an uncharacterized upstream regulator of YAP1 turnover in cancer cells of ovarian cancer and several other cancer types.

Deltacoronavirus HKU11, HKU13, PDCoV (HKU15) and HKU17 spike pseudoviruses enter avian DF-1 cells via clathrin-mediated endocytosis in a Rab5-, Rab7- and pH-dependent manner.

Porcine deltacoronavirus (PDCoV), also known as HKU15, is a swine enteropathogenic virus that is believed to have originated in birds. PDCoV belongs to the genus Deltacoronavirus (DCoV), the members of which have mostly been identified in diverse avian species. We recently reported that chicken or porcine aminopeptidase N (APN), the major cellular receptor for PDCoV, can mediate cellular entry via three pseudotyped retroviruses displaying spike proteins from three avian DCoVs (HKU11, HKU13, and HKU17).

Interplay of swine acute diarrhoea syndrome coronavirus and the host intrinsic and innate immunity.

Swine acute diarrhoea syndrome coronavirus (SADS-CoV), a novel HKU2-related coronavirus of bat origin, is a newly emerged swine enteropathogenic coronavirus that causes severe diarrhoea in piglets. SADS-CoV has a broad cell tropism with the capability to infect a wide variety of cells from human and diverse animals, which implicates its ability to hold high risks of cross-species transmission. The intracellular antiviral immunity, comprised of the intrinsic and innate immunity, represents the first line of host defence against viral infection prior to the onset of adaptive immunity.

Ultrasensitive "Hunter of Target": Rabbit Monoclonal Antibody-Based Competitive Lateral Flow Immunoassay for Saxitoxin.

The rapid, sensitive, and accurate detection of paralytic shellfish toxins (PSTs), such as saxitoxin (STX), is critical for protecting human health due to the frequent occurrence of toxic red tides. In this work, to address the low affinity of traditional mouse monoclonal antibodies (m-mAbs), rabbit monoclonal antibodies (r-mAbs) against STX were produced by a single B-cell sorting culture and a cross-selection strategy. The r-mAbs showed 100-fold improvement in sensitivity (IC = 0.

Ultrafast enzyme-responsive hydrogel for real-time assessment and treatment optimization in infected wounds.

Monitoring wound infection and providing appropriate treatment are crucial for achieving favorable outcomes. However, the time-consuming nature of laboratory culture tests may delay timely intervention. To tackle this challenge, a simple yet effective HDG hydrogel, composed of hydrogen peroxide (H₂O₂), dopamine, and GelMA polymer, is developed for the ultrafast detection and treatment of Staphylococcus aureus (SA) infections.

Calcium alginate reinforced zwitterionic double network hydrogel with mechanical robustness and antimicrobial activity for freshwater shrimp spoilage detection.

Hydrogel indicators promise to monitor food spoilage, but their poor mechanics can cause defects in transport. Herein, a novel zwitterionic double network (DN) hydrogel was developed by polymerizing arylamide and sulfobetaine methacrylate in an alginate-Ca system. This hydrogel exhibited enhanced mechanical properties, including a maximum 2087 % breaking elongation and 135 ± 12 kJ/m toughness, significantly outperforming the current zwitterionic DN hydrogels, which typically exhibit less than 1800 % breaking elongation, capable of supporting 150 g-136 times its own weight.

Enhanced Ocular Bioavailability and Prolonged Duration via Hydrophilic Surface Nanocomposite Vesicles for Topical Drug Administration.

Background: Internal ocular diseases, such as macular edema, uveitis, and diabetic macular edema require precise delivery of therapeutic agents to specific regions within the eye. However, the eye's complex anatomical structure and physiological barriers present significant challenges to drug penetration and distribution. Traditional eye drops suffer from low bioavailability primarily due to rapid clearance mechanisms.

Thymidine Phosphorylase Promotes Abdominal Aortic Aneurysm via VSMC Modulation and Matrix Remodeling in Mice and Humans.

Thymidine phosphorylase (TYMP) promotes platelet activation and thrombosis while suppressing vascular smooth muscle cell (VSMC) proliferation. Both processes are central to the development and progression of abdominal aortic aneurysms (AAAs). We hypothesize that TYMP plays a role in AAA development.

[Determination of 12 halogenated organic pollutants in edible fish by ultra performance liquid chromatography-high resolution mass spectrometry combined with ultrasound-assisted extraction and gel permeation chromatography purification].

Halogenated organic pollutants (HOPs) have attracted considerable attention owing to their persistence, bioaccumulation, and toxicity. The development of methods to detect HOPs in fish is challenging owing to the compositional complexity of fish matrices, which contain high levels of lipids and relatively low concentrations of HOPs. In addition, the lipophilicity of most HOPs renders their extraction difficult.

Synaptic basis of feature selectivity in hippocampal neurons.

A central question in neuroscience is how synaptic plasticity shapes the feature selectivity of neurons in behaving animals. Hippocampal CA1 pyramidal neurons display one of the most striking forms of feature selectivity by forming spatially and contextually selective receptive fields called place fields, which serve as a model for studying the synaptic basis of learning and memory. Various forms of synaptic plasticity have been proposed as cellular substrates for the emergence of place fields.

A20 negatively regulates necroptosis-induced microglia/macrophages polarization and mediates cerebral ischemic tolerance via inhibiting the ubiquitination of RIP3.

Neuronal necroptosis appears to be suppressed by the deubiquitinating enzyme A20 and is capable to regulate the polarization of microglia/macrophages after cerebral ischemia. We have demonstrated that hypoxic preconditioning (HPC) can alleviate receptor interacting protein 3 (RIP3)-induced necroptosis in CA1 after transient global cerebral ischemia (tGCI). However, it is still unclear whether HPC serves to regulate the phenotypic polarization of microglia/macrophages after cerebral ischemia by mitigating neuronal necroptosis.

Cardiovascular adverse events in patients with HER2-positive breast cancer treated with trastuzumab-drug conjugates : a Bayesian disproportional real world study for signal detection leveraging the FDA Adverse Event Reporting System (FAERS).

Background: The cardiovascular risk profiles of trastuzumab drug conjugates (T-DCs) in the treatment of Human Epidermal Growth Factor Receptor 2 (HER2)-positive breast cancer are uncertain. This study analyzed real-world adverse events data from the Food and Drug Administration (FDA) Adverse Event Reporting System (FAERS) to investigate the association between T-DCs and major adverse cardiovascular events (MACE).

Methods: We searched the FAERS database for reports of cardiovascular adverse events in patients with HER2-positive breast cancer receiving trastuzumab, ado-trastuzumab emtansine (T-DM1), and trastuzumab deruxtecan (T-Dxd).

Heterologous expression, purification and generation of specific antibodies for Annexin A8.

Annexin A8 (ANXA8) is a member of the Annexin gene family, with high levels of its mRNA and protein observed in various tumor tissues, making it a potential tumor biomarker. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) to specifically detect the presence and accurately determine the concentration of ANXA8. To this end, we expressed a series of proteins using both Pichia pastoris and Escherichia coli expression systems.

Construction of bFGF/heparin and FeO nanoparticles functionalized scaffolds aiming at vascular repair and magnetic resonance imaging monitoring.

This work develops a bioactive basic fibroblast growth factor (bFGF)/heparin and FeO nanoparticles (NPs) trifunctionalized degradable construct with the potential of using as a vascular tissue engineering scaffold with the aim of improving vascular repair and regeneration therapy. The covalent modification of heparin onto the poly(lactic acid) (PLA)-gelatin (Gel)-FeO (PGF) scaffold improves the hydrophilicity of the scaffold. Furthermore, the electrostatic adsorption of bFGF on heparin allows for a more consistent and prolonged release of bFGF in situ, hence increasing the stability and effectiveness of bFGF around the surrounding vascular tissues.

Ultraflexible Neural Electrodes Enabled Synchronized Long-Term Dopamine Detection and Wideband Chronic Recording Deep in Brain.

Ultraflexible neural electrodes have shown superior stability compared with rigid electrodes in long-term recordings, owing to their low mechanical mismatch with brain tissue. It is desirable to detect neurotransmitters as well as electrophysiological signals for months in brain science. This work proposes a stable electronic interface that can simultaneously detect neural electrical activity and dopamine concentration deep in the brain.

Development of a UPLC-MS/MS Method for Bioanalysis of Ethoxysanguinarine and Its Application in Pharmacokinetic Study of Ethoxysanguinarine Nanoemulsion.

Ethoxysanguinarine (ETSG), a benzophenanthridine alkaloid, exhibits diverse biological activities, including antibacterial, antifungal, anti-inflammatory, antioxidant, and anti-tumor effects. Despite these properties, limited research exists on ETSG in vivo pharmacokinetics due to its poor solubility and low bioavailability. In this study, we developed a rapid and specific UPLC-MS/MS method for ETSG bioanalysis.

The Co-Infection of ISKNV-II and RGNNV Resulting in Mass Mortality of Juvenile Asian Seabass (Lates calcarifer), Zhuhai, Southern China.

Infectious spleen and kidney necrosis virus (ISKNV) and nervous necrosis virus (NNV) are two common and important causative agents in marine-cultured fish. However, high viral loads of both ISKNV and NNV in the same clinical case is unusual. In this study, a mass mortality event of Asian seabass Lates calcarifer juveniles occurred in Zhuhai, the main Asian seabass cultured area in mainland China.

Biomagnification of polybrominated diphenyl ethers and alternative halogenated flame retardants through food chains of yellowfin tuna (Thunnus albacares) and their prey from the South China Sea and the associated potential health risk.

Polybrominated diphenyl ethers (PBDEs) and their alternative halogenated flame retardants (AHFRs) have gained global attention due to their ubiquitous occurrence, bioaccumulation, and toxic properties. However, the biomagnification of halogenated flame retardants (HFRs), particularly AHFRs, in various food chains is not yet well understood. In this study, yellowfin tuna (Thunnus albacares), along with its prey, flying squid (Sthenoteuthis oualaniensis) and round scad (Decapterus maruadsi), were sampled from the South China Sea (SCS) to investigate the biomagnification potential of PBDEs and AHFRs, including dechlorane plus (DP) and decabromodiphenyl ethane (DBDPE).

The role of chorion integrity on the bioaccumulation and toxicity of selenium nanoparticles in Japanese medaka (Oryzias latipes).

Selenium nanoparticles (nano-Se) have a wide range of biomedical and agricultural applications. However, there is little information on the potential toxicity of nano-Se once it enters the environment, particularly in fish. The first line of defense from contaminants that embryonic fish have is the chorion, but the degree to which the chorion protects the developing embryo is unknown.

The Effect of Caffeine Exposure on Sleep Patterns in Zebrafish Larvae and Its Underlying Mechanism.

The effect of caffeine on the behavior and sleep patterns of zebrafish larvae, as well as its underlying mechanisms, has been a topic of great interest. This study aimed to investigate the impact of caffeine on zebrafish larval sleep/wake behavior and the expression of key regulatory genes such as cAMP-response element binding protein (CREB) and adenosine (ADA) in the sleep pathway. To begin, the study determined the optimal dose and duration of caffeine exposure, with the optimal doses found to be 31.