561 results match your criteria: "Guangdong Institute of Gastroenterology[Affiliation]"

Systematic identification of secondary bile acid production genes in global microbiome.

mSystems

December 2024

State Key Laboratory of Genetic Engineering, Fudan Microbiome Center, School of Life Sciences, Fudan University, Shanghai, China.

Unlabelled: Microbial metabolism of bile acids (BAs) is crucial for maintaining homeostasis in vertebrate hosts and environments. Although certain organisms involved in bile acid metabolism have been identified, a global, comprehensive elucidation of the microbes, metabolic enzymes, and bile acid remains incomplete. To bridge this gap, we employed hidden Markov models to systematically search in a large-scale and high-quality search library comprising 28,813 RefSeq multi-kingdom microbial complete genomes, enabling us to construct a secondary bile acid production gene catalog.

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Anal fistula is a common disease with recurrent inflammation and accumulated exudate. Traditional treatments often fail to effectively eliminate inflammation and ensure adequate drainage, leading to prolonged healing time and a high recurrence rate. Herein, a new class of quaternized molecular brush-grafted injectable microgel (denoted as GAA@CNT-g-PVBTMA) is developed through thermal polymerization and mechanical fragmentation to promote the healing process of anal fistula.

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ASO Visual Abstract: An Organoid Model for the Therapeutic Effect of Hyperthermic Intraperitoneal Chemotherapy for Colorectal Cancer.

Ann Surg Oncol

November 2024

Department of Colorectal Surgery, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Medical Innovation Technology Transformation Center of Shenzhen Second People's Hospital, Shenzhen University, Shenzhen, China.

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Background: Approximately 25% of patients with stage III colorectal cancer experience liver metastasis after radical resection; however, there is currently a lack of methods to predict liver metastasis. This study aims to develop and validate a pathomics nomogram to predict liver metastasis in patients with stage III colorectal cancer.

Methods: A total of 318 enrolled patients were divided into three cohorts: a training cohort (n = 139), a validation cohort (n = 69), and an external cohort (n = 110).

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Metagenomic research faces a persistent challenge due to the low concordance across studies. While matching host confounders can mitigate the impact of individual differences, the influence of factors such as genetics, environment, and lifestyle habits on microbial profiles makes it exceptionally challenging to create fully matched cohorts. The microbial metabolic background, which modulates microbial composition, reflects a cumulative impact of host confounders, serving as an ideal baseline for microbial sample matching.

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An Organoid Model for the Therapeutic Effect of Hyperthermic Intraperitoneal Chemotherapy for Colorectal Cancer.

Ann Surg Oncol

November 2024

Department of Colorectal Surgery, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Medical Innovation Technology Transformation Center of Shenzhen Second People's Hospital, Shenzhen University, Shenzhen, China.

Background: Consensus regarding the hyperthermic intraperitoneal chemotherapy (HIPEC) for colorectal cancer (CRC) regimen remains elusive. In this study, patient-derived tumor organoids from CRC were utilized as a preclinical model for in vitro drug testing of HIPEC regimens commonly used in clinical practice. This approach was used to facilitate the clinical formulation of HIPEC.

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Raddeanin A Inhibits Colorectal Cancer Growth and Ameliorates Oxaliplatin Resistance Through the WNT/β-Catenin Signaling Pathway.

Cancer Biother Radiopharm

November 2024

Department of General Surgery (Colorectal Surgery), The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, China.

Chemotherapy based on oxaliplatin (OXA) is the first-line treatment for advanced colorectal cancer (CRC), and acquired resistance to OXA is the main reason for clinical treatment failure in CRC. To search for compounds that can reverse OXA resistance, we screened a small molecule inhibitor drug library and identified a drug, Raddeanin A (RA), that enhanced the anticancer effect of OXA. Using human CRC cell lines, CRC organoid models, and subcutaneous tumorigenic studies, we determined that RA inhibits the proliferation of CRC cells by promoting apoptosis and inducing cell cycle arrest.

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ASO Author Reflections: Organoid Model to Aid the Decision of Hyperthermic Intraperitoneal Chemotherapy for Colorectal Cancer.

Ann Surg Oncol

November 2024

Department of Colorectal Surgery, Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, Medical Innovation Technology Transformation Center of Shenzhen Second People's Hospital, Shenzhen University, Shenzhen, China.

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KRAS inhibitors may prevent colorectal cancer metachronous metastasis by suppressing TGF‑β mediated epithelial‑mesenchymal transition.

Mol Med Rep

January 2025

Guangdong Institute of Gastroenterology, Department of General Surgery, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, The Sixth Affiliated Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510655, P.R. China.

In colorectal cancer (CRC), KRAS mutations enhance metachronous metastasis, a condition without prognostic biomarkers or preventive measures. The present study demonstrated that KRAS mutation may be a risk factor for CRC metachronous metastasis through meta‑analysis of public databases. A risk scoring model was constructed using machine learning for predicting metachronous metastasis in KRAS‑mutant CRC.

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variant predicts disease progression in Chinese patients with Crohn's disease.

Gastroenterol Rep (Oxf)

November 2024

Department of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, P. R. China.

Article Synopsis
  • - The study investigates a genetic variant (rs6478109) in a specific gene associated with Crohn's disease (CD) and how it relates to intestinal fibrosis in Chinese patients.
  • - Results showed that patients with the GG genotype of this variant had a higher frequency of stricturing CD and higher expression of pro-fibrotic proteins compared to those with AA or AG genotypes.
  • - The findings suggest that the GG genotype could be a predictive marker for the progression of stricturing CD and highlights its role in promoting fibrosis-related protein expression.
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Targeting G-quadruplex by TMPyP4 for inhibition of colorectal cancer through cell cycle arrest and boosting anti-tumor immunity.

Cell Death Dis

November 2024

Guangdong Institute of Gastroenterology, Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Disease, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, China.

Article Synopsis
  • * Increased G4 formations in colorectal cancer cells are linked to more CD8 T cell infiltration, and the G4 ligand TMPyP4 has been shown to hinder cancer growth and stimulate immune responses.
  • * TMPyP4 not only stops cancer cell division but also boosts anti-tumor immunity by activating the cGAS-STING pathway, and its combination with anti-PD1 therapy shows enhanced effects against colorectal cancer.
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Recent Development of Fibrous Hydrogels: Properties, Applications and Perspectives.

Adv Sci (Weinh)

November 2024

Department of General Surgery (Colorectal Surgery), Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, P. R. China.

Article Synopsis
  • * The review explores various assembly methods that improve FGs' functionalities, including fiber cross-linking and supramolecular assembly, along with mechanisms such as creating ion carrier channels and in situ drug gelation.
  • * Applications for FGs include sensors, dressings, and tissue scaffolds, and the review concludes with insights on future challenges and innovations in the design and use of FGs.
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Neutrophil extracellular traps with low concentrations induce proliferation and migration of human fibroblasts via activating CCDC25/ILK/PI3K/AKT pathway.

Biochem Biophys Res Commun

December 2024

Medical Innovation Research Department, PLA General Hospital and PLA Medical College, 51 Fucheng Road, Haidian District, Beijing, 100048, China; Medical Sciences, 2019RU051, 51 Fucheng Road, Haidian District, Beijing, 100048, China. Electronic address:

Neutrophil plays an important role in the early stage of wound healing. Excessive neutrophil extracellular traps (NETs) which are produced by neutrophil have been proved as a significant unfavorable factor for wound healing by impairing the function of human skin fibroblasts. However, the effect of NETs with low concentrations on fibroblasts remains unclear.

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Generic Diagramming Platform (GDP): a comprehensive database of high-quality biomedical graphics.

Nucleic Acids Res

October 2024

School of Life Sciences, State Key Laboratory of Oncology in South China, Cancer Center, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University, Guangzhou 510060, China.

High-quality schematic illustrations are fundamental to the publication of scientific achievements in biomedical research, which are crucial for effectively conveying complex biomedical concepts. However, creating such illustrations remains challenging for many researchers due to the need to devote a significant amount of time and effort to accomplish it. To address this need, we present the Generic Diagramming Platform (GDP, https://BioGDP.

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Article Synopsis
  • Microwave ablation (MWA) is used to treat colorectal liver metastases (CRLM), but predicting tumor recurrence is challenging due to a lack of reliable markers.
  • This study analyzed data from 192 CRLM patients who underwent MWA to evaluate the role of the gamma-glutamyl transferase (GGT) to aspartate transaminase (AST) ratio (GSR) in predicting liver recurrence.
  • Results indicated that a high GSR is linked to poorer survival outcomes and associated with male patients and those with gallstones, suggesting it could serve as a useful prognostic factor for assessing patient risk.
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The impact of coronavirus disease 2019 (COVID-19) history on Crohn's disease (CD) is unknown. This investigation aimed to examine the effect of COVID-19 history on the disease course, oral-gut microbiota, and serum metabolomics in patients with CD. In this study, oral-gut microbiota and serum metabolomic profiles in 30 patients with CD and a history of mild COVID-19 (positive group, PG), 30 patients with CD without COVID-19 history (negative group, NG), and 60 healthy controls (HC) were assessed using 16S rDNA sequencing and targeted metabolomics.

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The human microbiome is a complex and dynamic system that plays important roles in human health and disease. However, there remain limitations and theoretical gaps in our current understanding of the intricate relationship between microbes and humans. In this narrative review, we integrate the knowledge and insights from various fields, including anatomy, physiology, immunology, histology, genetics, and evolution, to propose a systematic framework.

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Article Synopsis
  • - The study investigates the effectiveness and safety of combining anti-PD-1 therapy with chemotherapy and bevacizumab for patients with pMMR/MSS colorectal liver metastases, an area where traditional immunotherapy shows limited success.
  • - In a trial with 12 patients, the combination treatment achieved a 100% disease control rate and a 70% objective response rate, with some patients experiencing complete responses in their tumors.
  • - Results suggest that this combined treatment approach is safe, with no serious side effects reported, and may significantly improve tumor regression and the likelihood of patients being disease-free post-treatment.
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Targeting PRMT3 impairs methylation and oligomerization of HSP60 to boost anti-tumor immunity by activating cGAS/STING signaling.

Nat Commun

September 2024

State Key Laboratory of Oncology in South China, Guangdong Provincial Clinical Research for Cancer, Sun Yat-Sen University Cancer Center, Guangzhou, China.

Immune checkpoint blockade (ICB) has emerged as a promising therapeutic option for hepatocellular carcinoma (HCC), but resistance to ICB occurs and patient responses vary. Here, we uncover protein arginine methyltransferase 3 (PRMT3) as a driver for immunotherapy resistance in HCC. We show that PRMT3 expression is induced by ICB-activated T cells via an interferon-gamma (IFNγ)-STAT1 signaling pathway, and higher PRMT3 expression levels correlate with reduced numbers of tumor-infiltrating CD8 T cells and poorer response to ICB.

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Article Synopsis
  • This study explores how radiogenomic analysis can identify imaging biomarkers linked to DNA methylation in gliomas to improve molecular diagnosis and treatment.* -
  • Machine learning techniques were applied to analyze MRI features from 146 glioma patients, resulting in models (MoRad) that accurately profile various molecular features, particularly global DNA methylation.* -
  • Findings show that global DNA methylation correlates with certain MRI features, histological grades, and various molecular and immunological factors, indicating its potential as a quantitative imaging biomarker.*
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The involvement of oral bacteria in inflammatory bowel disease.

Gastroenterol Rep (Oxf)

August 2024

Department of Gastroenterology, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong, P. R. China.

Microorganisms play an important role in the pathogenesis of inflammatory bowel disease (IBD). The oral cavity, the second-largest microbial niche, is connected to the gastro-intestinal tract. Ectopic gut colonization by oral microbes is a signature of IBD.

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Nilotinib boosts the efficacy of anti-PDL1 therapy in colorectal cancer by restoring the expression of MHC-I.

J Transl Med

August 2024

Department of Clinical Laboratory, The Sixth Affiliated Hospital, Sun Yat-Sen University, Guangzhou, 510655, Guangdong, China.

Article Synopsis
  • Immune checkpoint inhibitors (ICIs) are transforming cancer treatment, but many colorectal cancer (CRC) patients still don't respond; improving MHC-I expression could enhance tumor immunity and ICI effectiveness.
  • Researchers tested drug candidates, particularly nilotinib, using various techniques to confirm its ability to boost MHC-I expression and enhance CD8 T-cell activity against CRC.
  • Nilotinib increases MHC-I levels through the cGAS-STING-NF-κB pathway, lowers MHC-I degradation by inhibiting PCSK9, and the study suggests combining it with anti-PDL1 therapy may improve CRC treatment outcomes.*
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Purpose: Cancer has become a significant major public health concern, making the discovery of new cancer markers or therapeutic targets exceptionally important. Elevated expression of tumor necrosis factor receptor superfamily member 12A (TNFRSF12A) expression has been observed in certain types of cancer. This project aims to investigate the function of TNFRSF12A in tumors and the underlying mechanisms.

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Diminished representation of vitamin-B12-producing bacteria in constipated elders with frailty.

iScience

August 2024

Digestive Disease Center, Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China.

Article Synopsis
  • A study explored the relationship between constipation, frailty, and changes in gut microbiota in elderly participants, aiming to identify unique microbial signatures.
  • Stool samples from 61 individuals were analyzed, revealing that constipated elders with frailty had less diverse gut microbiomes compared to those without frailty and healthy subjects.
  • The research identified specific biomarkers and pathways related to vitamin B12 synthesis that were lower in frail constipated individuals, highlighting the potential for these microbial differences in diagnostics and treatments.
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