An electrochemical immunosensor for the detection of Glypican-3 based on enzymatic ferrocene-tyramine deposition reaction.

An ultrasensitive electrochemical immunosensor based on signal amplification of the deposition of the electroactive ferrocene-tyramine (Fc-Tyr) molecule, catalyzed by horseradish peroxidase (HRP), was constructed for the detection of the liver cancer marker Glypican-3 (GPC3). Functional electroactive molecule Fc-Tyr is reported to exhibit both the enzymatic cascade catalytic activity of tyramine signal amplification (TSA) and the excellent redox properties of ferrocene. In terms of design, the low matrix effects inherent in using the magnetic bead platforms, a quasi-homogeneous system, allowed capturing the target protein GPC3 without sample pretreatment, and loading HRP to trigger the TSA, which induced a large amount of Fc-Tyr deposited on the electrode surface layer by layer as a signal probe for the detection of GPC3.

Changes in structure and physicochemical properties of Sophora japonica f. pendula galactomannan in late growth stage.

Galactomannan (GM) has been widely applied in food and other fields due to its appealing physicochemical properties. In this work, considering the changes in structural and physicochemical properties of Sophora japonica f. pendula (SJ-GM) with very high mannose to galactose (M/G) ratio in the late deposition stage, extensive exploration is conducted.

Superior graphdiyne self-powered biosensing platform with highly sensitivity and reliability for dual-mode detection of MicroRNA by integrating T7 Exonuclease and 3D DNA walker induced rolling circle amplification.

A highly sensitivity self-powered biosensor is developed based on T7 exonuclease (T7 Exo) and 3D DNA walker induced rolling circle amplification (RCA) for electrochemical/colorimetric dual-mode detection of microRNA-21 (miRNA-21) with improved reliability. Taking its advantage of fascinating properties, such as high structure defects and good conductivity, graphdiyne is prepared and used to prepare high-performance enzyme biofuel cell. T7 Exo-assisted 3D DNA walker target recognition triggers RCA reaction to obtain a significantly amplified signal response.

Establishment of a biomarker of peripheral stress in opioid addicts based on the hypothalamic-pituitary-adrenal axis-The improvement effect of exercise.

Purpose: This study aims to investigate the relationship between peripheral blood oxytocin (OT), vasopressin (AVP), and subjectively perceived stress and cortisol in male opioid addicts based on the hypothalamic-pituitary-adrenal (HPA) axis. We also investigate the impact of exercise on reducing subjectively perceived stress, craving level, negative reinforcement, anxiety, sleep quality, plasma OT, AVP, and cortisol levels.

Methods: Participants were divided into 28 subjects in the low-stress control group (LSC group), 29 subjects in the medium-high stress control group (MTHSC group), and 28 subjects in the moderate-high-stress exercise group (MTHSE group), based on their subjectively perceived stress levels.

Facile Access to Galactomannan Hydrogel with Rapid Self-Repair Capacity and Multicyclic Water-Retaining Performance of Sandy Soil.

Sandy soil has poor water-holding performance, making it difficult for plants to survive, which worsens the deterioration of the ecological environment. Therefore, borax cross-linked galactomannan hydrogel (GMGH) was prepared, and its practicability as a water-retaining agent was analyzed. GMGH exhibited fast self-healing performance (150 s, ≈100%) and a high swelling index (88.

An Improved Chimp-Inspired Optimization Algorithm for Large-Scale Spherical Vehicle Routing Problem with Time Windows.

The vehicle routing problem with time windows (VRPTW) is a classical optimization problem. There have been many related studies in recent years. At present, many studies have generally analyzed this problem on the two-dimensional plane, and few studies have explored it on spherical surfaces.

Preparation of highly efficient p-doped porous camellia shell-based activated carbon and its adsorption of carotenoids in camellia oil.

The vegetable oil industry is limited by the high cost of the refining process, and the camellia shells (CS) are beneficial to the development of the industry as a biomass raw material for camellia oil decolorization. In this study, CS-based p-doped porous activated carbon (CSHAC) obtained after the pyrolysis of HPO-laden CS-hydrochar (CSH) was used for the adsorption of carotenoids in camellia oil. The results showed that the adsorption efficiency of CSHAC for carotenoids was 96.

Acinetobacter faecalis Sp. Nov., Isolated from Elephant Faeces.

A Gram-negative coccobacillus, YIM 103518, isolated from wild elephant feces in Xishuangbanna, Yunnan Province, West China, was characterized and identified using a polyphasic taxonomic approach. The strain was strictly aerobic, non-motile, catalase-positive and oxidase-negative, colonies were round, convex, smooth, and pale yellow. The strain growth at 4-40 ℃ (optimum, 28 ℃), pH 6.

An Improved Density Peak Clustering Algorithm for Multi-Density Data.

Density peak clustering is the latest classic density-based clustering algorithm, which can directly find the cluster center without iteration. The algorithm needs to determine a unique parameter, so the selection of parameters is particularly important. However, for multi-density data, when one parameter cannot satisfy all data, clustering often cannot achieve good results.

Novel antioxidant peptides from MFGM protein Hydrolysates: Separation, identification and effects on Dexamethasone-induced mitochondrial dysfunction.

Milk fat globule membrane (MFGM) protein is a complex milk protein system with antioxidant property, which can alleviate skeletal muscle dysfunction caused by oxidative stress. In this study, peptide products of MFGM protein obtained through in vitro digestion were isolated and purified, and the composition and antioxidant activities of MFGM peptides (MFGMP) were identified and assessed using LC-MS/MS combined with molecular docking and in vitro approach. Three novel antioxidant peptides TGIIT, YAR and YYK were identified from MFGMPF1, among which TGIIT and YAR exhibited excellent antioxidant effects and protected dexamethasone (Dex)-induced L6 cells by enhancing mitochondrial function and biogenesis involving modulating Sirt-1/PGC-1α signaling pathway.

Production of xylooligosaccharides from Camellia oleifera Abel fruit shell using a shell-based solid acid catalyst.

This study aimed to produce xylooligosaccharides (XOS) from Camellia oleifera Abel fruit shell (CFS) using a shell-based solid acid derived from CFS (CFS-BSA). CFS-BSA preparation was optimized by incomplete carbonization at 450 °C for 1 h, followed by sulfonation at 130 °C for 8 h to yield a -SOH functional group concentration of 1.04 mmol/g.

Does retail investors beat institutional investors?--Explanation of game stop's stock price anomalies.

This paper studies the relation of information cost, retail investor sentiment and asset pricing. Our motivation to study this model is to learn why retail investors could move asset price away from fundamental values. In the model, the institutional investors are pessimistic and the retail investors are optimistic, the ratio of the expected utility of informed and rational but uninformed institutional investors increases first and then decreases as the cost of information increases.

Biochemical purification and characterization of a truncated acidic, thermostable chitinase from marine fungus for N-acetylglucosamine production.

N-acetylglucosamine (GlcNAc) is widely used in nutritional supplement and is generally produced from chitin using chitinases. While most GlcNAc is produced from colloidal chitin, it is essential that chitinases be acidic enzymes. Herein, we characterized an acidic, highly salinity tolerance and thermostable chitinase ChiJ, identified from the marine fungus df673.

Dietary iron affects lipid deposition, nutritional element, and muscle quality in coho salmon ().

We investigated effect of dietary iron (Fe) on the lipid deposition, nutritional element, and muscle quality in coho salmon. Four level Fe diets at 23.7, 46.

Modulating break types induces divergent low band EEG processes during post-break improvement: A power spectral analysis.

Conventional wisdom suggests mid-task rest as a potential approach to relieve the time-on-task (TOT) effect while accumulating evidence indicated that acute exercise might also effectively restore mental fatigue. However, few studies have explored the neural mechanism underlying these different break types, and the results were scattered. This study provided one of the first looks at how different types of fatigue-recovery break exerted influence on the cognitive processes by evaluating the corresponding behavioral improvement and neural response (EEG power spectral) in a sustained attention task.

Preparation of quaternary ammonium magnetic chitosan microspheres and their application for Congo red adsorption.

In this study, novel green in situ quaternary-ammonium-functionalized magnetic chitosan microspheres (IQMCM) were synthesised. The resulting adsorbent was characterised by Fourier transform infrared spectroscopy, X-ray diffraction, thermogravimetric analysis, vibrating sample magnetometry, X-ray photoelectron spectroscopy, and zeta potential analysis. The characterization results indicated that the IQMCMs possessed spherical morphology and superior specific thermal stability, magnetic properties, and surface charges.

Correction: Critical size effect for the surface heat capacities of nano-CdS: theoretical and experimental studies.

Correction for 'Critical size effect for the surface heat capacities of nano-CdS: theoretical and experimental studies' by Shengjiang Zhang , , 2022, , 6193-6207, https://doi.org/10.1039/D1CP04619E.

Novel glycosylation zinc(II)-cryptolepine complexes perturb mitophagy pathways and trigger cancer cell apoptosis and autophagy in SK-OV-3/DDP cells.

With the aim of shedding some light on the mechanism of action of zinc(II) complexes in antiproliferative processes and molecular signaling pathways, three novel glycosylated zinc(II)-cryptolepine complexes, i.e., [Zn(QA1)Cl] (Zn(QA1)), [Zn(QA2)Cl] (Zn(QA2)), and [Zn(QA3)Cl] (Zn(QA3)), were prepared by conjugating a glucose moiety with cryptolepine, followed by complexation of the resulting glycosylated cryptolepine compounds N-((1-(2-morpholinoethyl)-1H-1,2,3-triazol-4-yl)methyl)-benzofuro[3,2-b]quinolin-11-amine (QA1), 2-(4-((benzofuro[3,2-b]quinolin-11-ylamino)methyl)-1H-1,2,3-triazol-1-yl)ethan-1-ol (QA2), and (2S,3S,4R,5R,6S)-2-(4-((benzofuro[3,2-b]quinolin-11-ylamino)-methyl)-1H-1,2,3-triazol-1-yl)-6-(hydroxymethyl)tetrahydro-2H-pyran-3,4,5-triol (QA3) with zinc(II), and their anticancer activity was evaluated.

Quantum-Inspired Moth-Flame Optimizer With Enhanced Local Search Strategy for Cluster Analysis.

Clustering is an unsupervised learning technique widely used in the field of data mining and analysis. Clustering encompasses many specific methods, among which the K-means algorithm maintains the predominance of popularity with respect to its simplicity and efficiency. However, its efficiency is significantly influenced by the initial solution and it is susceptible to being stuck in a local optimum.

High school English-as-a-foreign-language teachers' emotional labor and job satisfaction: A latent profile analytical approach.

Previous studies have primarily used variable-centered approaches to explore correlations between English-as-a-foreign-language (EFL) teachers' emotional labor and outcome variables. A fundamental but unresolved question is whether teachers employ multiple emotional labor strategies in the workplace. This study used the latent profile analysis (LPA) to explore the profiles of EFL teachers' emotional labor and the relationship between the profiles and job satisfaction based on a questionnaire survey of 365 high school EFL teachers in China.

Identification of as the causative agent of leaf spot disease on in China.

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TonB systems are required for Aeromonas hydrophila motility by controlling the secretion of flagellin.

The TonB system is required for the active transport of iron compounds across the outer membrane in Gram-negative bacteria. Our previous data indicated that three TonB systems act coordinately to contribute to the motility of Aeromonas hydrophila NJ-35. In this study, we found that flagellum biogenesis was defective in the ΔtonB123 mutant.

CRISPR Contributes to Adhesion, Invasion, and Biofilm Formation in Streptococcus agalactiae by Repressing Capsular Polysaccharide Production.

The clustered regularly interspaced palindromic repeat (CRISPR)-associated (Cas) system functions classically as a prokaryotic defense system against invading mobile genetic elements, such as phages, plasmids, and viruses. Our previous study revealed that CRISPR deletion caused increased transcription of capsular polysaccharide (CPS) synthesis-related genes and severely attenuated virulence in the hypervirulent piscine Streptococcus agalactiae strain GD201008-001. Here, we found that CRISPR deficiency resulted in reduced adhesion, invasion, and biofilm formation abilities in this strain by upregulating the production of CPS.

A novel synthesis method of cyclopentadecanone and cyclopentadecanolide from vegetable oil.

Malania oleifera Chum is a unique renewable plant resource in China, its fruit oil has a high content of 15-tetracosenic acid, and it is a good raw material for the synthesis of cyclopentadecanone and cyclopentadecanolide. A Novel synthesis method of cyclopentadecanone and cyclopentadecanolide from vegetable oil was designed, and the synthesis yields of c cyclopentadecanone and cyclopentadecanolide were 38.5% and 63.