Functionalisation of polyhydroxybutyrate for diagnostic uses.

Polyhydroxybutyrate (PHB) is a biodegradable and biocompatible biopolyester, naturally produced and self-assembled as spherical inclusions inside bacteria. These PHB particles contain a hydrophobic PHB core covalently coated with PHB synthase (PhaC), which serves as an anchoring linker for foreign proteins of interest. Protein engineering of PhaC enables the display of biologically active protein functions on the surface of PHB particles suitable for different applications.

S-MGBs bearing amidine tail groups are potent, selective antiplasmodial agents.

There were an estimated 249 million cases of malaria globally in 2022, causing approximately 608 000 deaths. Most of these are attributed to infection by . Strathclyde minor groove binders (S-MGBs) are a promising new class of anti-infective agent that have been shown to be effective against many infectious organisms, including .

Targeting lysosomes by design: novel -acridine thiosemicarbazones that enable direct detection of intracellular drug localization and overcome P-glycoprotein (Pgp)-mediated resistance.

Innovative -acridine thiosemicarbazones (NATs) were designed along with their iron(iii), copper(ii), and zinc(ii) complexes. Lysosomal targeting was promoted by specifically incorporating the lysosomotropic Pgp substrate, acridine, into the thiosemicarbazone scaffold to maintain the tridentate N, N, S-donor system. The acridine moiety enables a significant advance in thiosemicarbazone design, since: (1) it enables tracking of the drugs by confocal microscopy using its inherent fluorescence; (2) it is lysosomotropic enabling lysosomal targeting; and (3) as acridine is a P-glycoprotein (Pgp) substrate, it facilitates lysosomal targeting, resulting in the drug overcoming Pgp-mediated resistance.

Identifying and embedding transferability in data-driven representations of chemical space.

Transferability, especially in the context of model generalization, is a paradigm of all scientific disciplines. However, the rapid advancement of machine learned model development threatens this paradigm, as it can be difficult to understand how transferability is embedded (or missed) in complex models developed using large training data sets. Two related open problems are how to identify, without relying on human intuition, what makes training data transferable; and how to embed transferability into training data.

Fragment-based drug discovery campaigns guided by native mass spectrometry.

Native mass spectrometry (nMS) is well established as a biophysical technique for characterising biomolecules and their interactions with endogenous or investigational small molecule ligands. The high sensitivity mass measurements make nMS particularly well suited for applications in fragment-based drug discovery (FBDD) screening campaigns where the detection of weakly binding ligands to a target biomolecule is crucial. We first reviewed the contributions of nMS to guiding FBDD hit identification in 2013, providing a comprehensive perspective on the early adoption of nMS for fragment screening.

Long-distance swimming by African lions in Uganda.

Earth's most imperiled and iconic wildlife are facing tough decisions under increasing human pressure and limited resources. Swimming across rivers and water bodies filled with high densities of predators may be one such example. In African lions , previous water crossings (recorded in the peer-reviewed and gray literature, on film, and found using Google Search, and YouTube) have recorded distances ranging from <10 to 100 m, with some resulting in mortality by Nile Crocodiles .

The thermal breadth of temperate and tropical freshwater insects supports the climate variability hypothesis.

Climate change involves increases in mean temperature and changes in temperature variability at multiple temporal scales but research rarely considers these temporal scales. The climate variability hypothesis (CVH) provides a conceptual framework for exploring the potential effects of annual scale thermal variability across climatic zones. The CVH predicts ectotherms in temperate regions tolerate a wider range of temperatures than those in tropical regions in response to greater annual variability in temperate regions.

Differential transmetallation of complexes of the anti-cancer thiosemicarbazone, Dp4e4mT: effects on anti-proliferative efficacy, redox activity, oxy-myoglobin and oxy-hemoglobin oxidation.

The di-2-pyridylthiosemicarbazone (DpT) analogs demonstrate potent and selective anti-proliferative activity against human tumors. The current investigation reports the synthesis and chemical and biological characterization of the Fe(iii), Co(iii), Ni(ii), Cu(ii), Zn(ii), Ga(iii), and Pd(ii) complexes of the promising second generation DpT analog, di-2-pyridylketone-4-ethyl-4-methyl-3-thiosemicarbazone (Dp4e4mT). These studies demonstrate that the Dp4e4mT Co(iii), Ni(ii), and Pd(ii) complexes display distinct biological activity those with Cu(ii), Zn(ii), and Ga(iii) regarding anti-proliferative efficacy against cancer cells and a detrimental off-target effect involving oxidation of oxy-myoglobin (oxy-Mb) and oxy-hemoglobin (oxy-Hb).

Co-designing an online treatment decision aid for men with low-risk prostate cancer: Navigate.

Objectives: To develop an online treatment decision aid (OTDA) to assist patients with low-risk prostate cancer (LRPC) and their partners in making treatment decisions.

Patients And Methods: , an OTDA for LRPC, was rigorously co-designed by patients with a confirmed diagnosis or at risk of LRPC and their partners, clinicians, researchers and website designers/developers. A theoretical model guided the development process.

Targeting Tumor Heterogeneity with Neoantigen-Based Cancer Vaccines.

Neoantigen-based cancer vaccines have emerged as a promising immunotherapeutic approach to treat cancer. Nevertheless, the high degree of heterogeneity in tumors poses a significant hurdle for developing a vaccine that targets the therapeutically relevant neoantigens capable of effectively stimulating an immune response as each tumor contains numerous unique putative neoantigens. Understanding the complexities of tumor heterogeneity is crucial for the development of personalized neoantigen-based vaccines, which hold the potential to revolutionize cancer treatment and improve patient outcomes.

Evaluating and optimising performance of multi-species call recognisers for ecoacoustic restoration monitoring.

Monitoring the effect of ecosystem restoration can be difficult and time-consuming. Autonomous sensors, such as acoustic recorders, can aid monitoring across long time scales. This project successfully developed, tested and implemented call recognisers for eight species of frog in the Murray-Darling Basin.

Polymeric epitope-based vaccine induces protective immunity against group A Streptococcus.

Group A Streptococcus (Strep A) is a life-threatening human pathogen with no licensed vaccine. Here, we used a biopolymer particle (BP) approach to display repeats of Strep A vaccine candidate peptides p*17 and K4S2 derived from M and non-M protein, respectively. BPs densely displaying both peptides (BP-p*17-S2) were successfully assembled in one-step inside an engineered endotoxin-free Escherichia coli strain.

Influence of cultivar and fertilization treatment on the yield and leaf nutrients content of apple ( Borkh.).

Fertilisation strategy can have a big impact on apple ( Borkh) yield, with considerable environmental and economic implications. This research paper presents the yield and leaf nutrients content of three apple cultivars fertilised with three treatment regimes over 2 years (2020-202) in Bosnia and Herzegovina. The specific apple cultivars investigated were: Jonagold Decosta, Red Idared and Gala Schnitzer®Schniga, each fertilised with three treatments: T (control‒without fertilization); T (300 kg ha NPK (6:18:36) + 150 kg/ha N (calcium ammonium nitrate‒CAN)) and T (foliar nutrition‒mixture organic-mineral fertilizer commercially named "FitoFert Kristal" (0.

First mitogenome phylogeny of the sun bear reveals a deep split between Indochinese and Sundaic lineages.

The sun bear is one of the most endangered ursids, and to date classification of sun bear populations has been based almost exclusively on geographic distribution and morphology. The very few molecular studies focussing on this species were limited in geographic scope. Using archival and non-invasively collected sample material, we have added a substantial number of complete or near-complete mitochondrial genome sequences from sun bears of several range countries of the species' distribution.

Rhenium anchored TiCT (MXene) nanosheets for electrocatalytic hydrogen production.

Atomically thin TiCT (MXene) nanosheets with rich termination groups, acting as active sites for effective functionalization, are used as an efficient solid support to host rhenium (Re) nanoparticles for the electrocatalytic hydrogen evolution reaction (HER). The newly designed electrocatalyst - Re nanoparticles anchored on TiCT MXene nanosheets (Re@TiCT ) - exhibited promising catalytic activity with a low overpotential of 298 mV to achieve a current density of 10 mV cm, while displaying excellent stability. In comparison, the pristine TiCT MXene requires higher overpotential of 584 mV to obtain the same current density.

More from less: improving solar steam generation by selectively removing a portion of evaporation surface.

Using minimal photothermal material to achieve maximum evaporation rate is extremely important for practical applications of interfacial solar evaporation technology. In this work, we found that with the increase in the size of evaporation surfaces, the evaporation rate decreased. Both experimental and numerical simulation results confirmed that when the evaporation surface size increased, the middle portion of the evaporation surface acted as a "dead evaporation zone" with little contribution to water evaporation.

Light Therapy to Improve Sleep Quality in Older Adults Living in Residential Long-Term Care: A Systematic Review.

Objectives: Because light can regulate sleep rhythms, numerous studies have investigated whether light therapy can improve sleep disorders in older people, but its efficacy remains controversial. Therefore, this systematic review aimed to examine and summarize current evidence about the efficacy of light therapy to improve sleep for older people in residential long-term care.

Design: Systematic review.

Public hesitancy to COVID-19 vaccine and the role of pharmacists in addressing the problem and improving uptake.

COVID-19 is one of the worst pandemics in recent human history, causing huge health, economic, and psychosocial damage. Since the pandemic hit, several unsubstantiated claims regarding exposure, transmission and management have been disseminated. Misinformation and associated public confusion now extend to the COVID-19 vaccines, spanning from claims based on possible links between some vaccine types and rare blood clots, to baseless claims.

The thiosemicarbazone, DpC, broadly synergizes with multiple anti-cancer therapeutics and demonstrates temperature- and energy-dependent uptake by tumor cells.

Background: The di-2-pyridylketone thiosemicarbazones, di-2-pyridylketone-4,4-dimethyl-3-thiosemicarbazone (Dp44mT) and di-2-pyridylketone-4-cyclohexyl-4-methyl-3-thiosemicarbazone (DpC), demonstrate potent and selective anti-tumor activity. In fact, DpC entered Phase I clinical trials for advanced and resistant tumors.

Methods: This investigation examined the activity of these thiosemicarbazones in five tumor cell-types compared to nine clinically used chemotherapeutics and also in combination with these drugs.

Genetic guidelines for translocations: Maintaining intraspecific diversity in the lion ().

Conservation translocations have become an important management tool, particularly for large wildlife species such as the lion (). When planning translocations, the genetic background of populations needs to be taken into account; failure to do so risks disrupting existing patterns of genetic variation, ultimately leading to genetic homogenization, and thereby reducing resilience and adaptability of the species. We urge wildlife managers to include knowledge of the genetic background of source/target populations, as well as species-wide patterns, in any management intervention.

Estimating viral prevalence with data fusion for adaptive two-phase pooled sampling.

The COVID-19 pandemic has highlighted the importance of efficient sampling strategies and statistical methods for monitoring infection prevalence, both in humans and in reservoir hosts. Pooled testing can be an efficient tool for learning pathogen prevalence in a population. Typically, pooled testing requires a second-phase retesting procedure to identify infected individuals, but when the goal is solely to learn prevalence in a population, such as a reservoir host, there are more efficient methods for allocating the second-phase samples.