Can the agrochemical Oryzemate treatment control the uptake of pyrene by Cucurbita pepo through the regulation of major latex-like proteins?

Members of the Cucurbitaceae family accumulate several hydrophobic organic pollutants in their above-ground parts at high concentrations. Major latex-like proteins (MLPs) identified in Cucurbita pepo bind to hydrophobic organic pollutants, such as pyrene and dieldrin, in roots, forming complexes that are transported via xylem vessels to the above-ground plant parts. However, soil remediation of hydrophobic organic pollutants utilizing MLPs has not been established.

Chitin promotes equol production via n-acetylglucosamine in human fecal cultures.

Objectives: Equol is a metabolite of isoflavone. Here, we investigated the effect of dietary fiber on equol-producing microbiota.

Methods: Human feces was anaerobically cultured in Gifu Anaerobic Medium (GAM) containing 1 mg/L daidzein supplemented with 1 % (w/v) dietary fiber (pectin, chitin, or resistant starch [RS]).

Modulation of the Gut-Lung Axis by Water Kefir and Kefiran and Their Impact on Toll-like Receptor 3-Mediated Respiratory Immunity.

The beneficial effect of milk kefir on respiratory heath has been previously demonstrated; however, water kefir and kefiran in the context of respiratory viral infections have not been investigated. Water kefir and kefiran could be alternatives to milk kefir for their application in persons with lactose intolerance or milk allergy and could be incorporated into vegan diets. Using mice models, this work demonstrated that the oral administration of water kefir or kefiran can modulate the respiratory Toll-like receptor (TLR3)-mediated innate antiviral immunity and improve the resistance to respiratory syncytial virus (RSV) infection.

Black Soybean Seed Coat Extract Suppresses Gut Tumorigenesis by Augmenting the Production of Gut Microbiota-Derived Short-Chain Fatty Acids.

Background: Proanthocyanidins (PACs) from black soybean seed coat have antioxidant and anti-tumorigenic properties. We investigated the anti-tumor properties and mechanisms of action of PACs on colorectal cancer (CRC).

Methods: We fed the APC mice, which are highly susceptible to spontaneous intestinal adenoma formation, diets supplemented with or without PACs for 7 weeks and assessed adverse effects, the number and size of intestinal polyps, and the expression of pro- and anti-proliferative proteins in the intestine.

Fermented Rice Bran Mitigated the Syndromes of Type 2 Diabetes in KK- Mice Model.

Diabetes is a devastating disease that causes millions of deaths. Fermented rice bran (FRB), made by fermenting rice bran with and a mixture of lactic acid bacteria, was hypothesized to b able to improve diabetes-related symptoms. This study aimed to investigate the effects of FRB supplementation in mitigating type 2 diabetes symptoms and identifying FRB bioactive compounds.

Mechanism of lipid peroxidation of liposomes by cold atmospheric pressure plasma jet irradiation.

Liposome lipid peroxidation induced by cold atmospheric pressure plasma jet (CAPPJ) irradiation was investigated. The formation of thiobarbituric acid reactive substances (TBARS), an indicator of lipid peroxidation final products, as a function of irradiation was observed. Lipid radicals, peroxidation reaction intermediates generated by CAPPJ irradiation, were confirmed by increased NBD-pen fluorescence intensity.

Application of High-pressure Carbon Dioxide in Japanese Anchovy Waste Recycling by Enzymatic Hydrolysis.

Anchovy waste, a protein resource with high nutritional value and potential for recycling with a relatively high economic effect, is essential for the Sustainable Development Goals of the United Nations. Preventing microbial contamination during the recycling process, through enzymatic hydrolysis, ensures the safety of recycled products. High-pressure carbon dioxide is a novel non-thermal decontamination technology, which inactivates cells by breaking their membranes.

produced by double saccharification contains more isomaltose and modifies the gut microbiota in mice.

which is made from rice and rice (a product of ), is a traditional Japanese beverage that has glucose as its main component. It also contains isomaltose, which has been reported to have various functionalities related to gut health. In the present study, we attempted to produce with a higher concentration of isomaltose without using any additives by focusing on the saccharification step of rice production as a means of creating new value for Two types of rice that were obtained at different fermentation time points were used, and we changed the saccharification process from the usual one step of saccharification to two steps of saccharification using a different type of for each step.

Intracellular abundance, localization, and enzymatic activity of a saxitoxin biosynthesis enzyme, SxtG, in two sister subclones of the dinoflagellate Alexandrium catenella with extremely different levels of paralytic shellfish toxins.

Paralytic shellfish poisoning is caused by saxitoxin (STX), and its analogues (paralytic shellfish toxins (PSTs)) produced by marine dinoflagellates. SxtA and SxtG are the most essential enzymes in STX biosynthesis. Previous studies investigated the abundance and subcellular localization (i.

The mechanism of allosteric regulation of calcium-independent phospholipase A by ATP and calmodulin binding to the ankyrin domain.

Group VIA calcium-independent phospholipase A (iPLA) is a member of the PLA superfamily that exhibits calcium-independent activity in contrast to the other two major types, secreted phospholipase A (sPLA) and cytosolic phospholipase A (cPLA), which both require calcium for their enzymatic activity. Adenosine triphosphate (ATP) has been reported to allosterically activate iPLA, and this has now been verified with a lipidomics-based mixed-micelle assay, but its mechanism of action has been unknown. Hydrogen/deuterium exchange mass spectrometry (HDX-MS) was employed to identify ATP interaction peptide regions located within the ankyrin repeat domain at which ATP interacts.

Contribution of irrigation to the production of maize, wheat, and rice in the major global producing countries.

This study offers new insights into the heterogeneity behind the widely accepted notion that irrigated crops contribute 40% to global food production. It also highlights the potential of irrigation to mitigate the negative effects of climate change on crop yields.

Loss of cytoplasmic actin filaments raises nuclear actin levels to drive INO80C-dependent chromosome fragmentation.

Loss of cytosolic actin filaments upon TORC2 inhibition triggers chromosome fragmentation in yeast, which results from altered base excision repair of Zeocin-induced lesions. To find the link between TORC2 kinase and this yeast chromosome shattering (YCS) we performed phosphoproteomics. YCS-relevant phospho-targets included plasma membrane-associated regulators of actin polymerization, such as Las17, the yeast Wiscott-Aldrich Syndrome protein.

Reticulate Evolution in Japanese Sceptridium (Ophioglossaceae), including diploid, tetraploid, and hexaploid species.

Homospory represents an intriguing reproductive strategy, including the potential for gametophytic selfing, considered the ultimate form of selfing. The plants of the fern genus Sceptridium (Ophioglossaceae) are generally considered to be predominantly selfing, making them suitable for analyzing speciation patterns and intraspecific variation characteristic of homosporous plants. The Sceptridium species in Japan not only exhibit variations in ploidy, including diploids, tetraploids, and hexaploids, but also pose taxonomic challenges due to morphologically distinctive forms that are difficult to be assigned to previously described species.

PRDX6 dictates ferroptosis sensitivity by directing cellular selenium utilization.

Selenium-dependent glutathione peroxidase 4 (GPX4) is the guardian of ferroptosis, preventing unrestrained (phospho)lipid peroxidation by reducing phospholipid hydroperoxides (PLOOH). However, the contribution of other phospholipid peroxidases in ferroptosis protection remains unclear. We show that cells lacking GPX4 still exhibit substantial PLOOH-reducing capacity, suggesting a contribution of alternative PLOOH peroxidases.

Freeze-dried fecal microorganisms as an effective biomaterial for the treatment of calves suffering from diarrhea.

Fecal microbiota transplantation (FMT) is a therapeutic modality for treating neonatal calf diarrhea. Several practical barriers, including donor selection, fecal collection, and a limited timeframe for FMT, are the main constraints to using fresh feces for implementing on-farm FMT. We report the utility of FMT with pretreated ready-to-use frozen (F) or freeze-dried (FD) microorganisms for treating calf diarrhea.

Dynamics of blood Taurine concentration and its correlation with nutritional and physiological status during the fattening period of Japanese black cattle.

Taurine, biosynthesized from methionine or cysteine in the liver, plays a crucial regulatory role in bile acid conjugation, antioxidant effects, and glucose and cholesterol metabolism. This may influence the metabolic changes associated with fat accumulation in beef cattle. However, the physiological role of taurine in this species has not been fully elucidated.

Conservation units and the origin of planted individuals of an endangered endemic species Lobelia boninensis in the Ogasawara Islands.

The Ogasawara Islands, one of the UNESCO World Natural Heritage Site located about 1000 km south of Japan's main island, harbor numerous endemic species, many of which are as endangered. One of the endemic plant species, Lobelia boninensis, found in the Mukojima, Chichijima, and Hahajima archipelagoes, is endangered due to predation. As part of conservation efforts, translocation is now underway, especially on Chichijima.

Efficacy of L-arabinose in managing cucumber Fusarium wilt and the underlying mechanism of action.

Background: Cucumber Fusarium wilt (CFW), triggered by Fusarium oxysporum f. sp. cucumerinum, leads to substantial yield reductions in global cucumber (Cucumis sativus L.

Methods of Calculating Prediction Error Variance and Prediction Accuracy for Restricted Best Linear Unbiased Prediction of Breeding Values.

Prediction error variance (PEV) and prediction accuracy (PA) of breeding values (BVs) are essential for formulating breeding plans and predicting response to selection. However, restricted best linear unbiased prediction method (RBLUP method) carries many unknowns: in particular, the formulas for calculating PEV and PA are not clear. New findings were obtained using the RBLUP method.

State analysis of a biobased hydrogel subjected to freeze and thaw processes by X ray absorption spectroscopy using synchrotron radiation.

Freezing hydrogels can compromise their network structures and modify their properties as a result of ice crystal formation. Therefore, understanding the internal structure, including ice crystals and the state of chemical components within hydrogels, is essential. In this study, we evaluated the elemental distribution in bio-based hydrogels subjected to freezing-thaw process using X-ray absorption spectroscopy with synchrotron radiation.

Development and characterization of expressed sequence tag-simple sequence repeat markers for the near-threatened halophyte Limonium tetragonum (Thunb.) A. A. Bullock (Plumbaginaceae).

Next-generation RNA sequencing analysis was performed to develop 13 novel expressed sequence tag-simple sequence repeat markers to evaluate the genetic variation in the near-threatened halophyte Limonium tetragonum (Thunb.) A. A.

A cellulose-binding domain specific for native crystalline cellulose in lytic polysaccharide monooxygenase from the brown-rot fungus Gloeophyllum trabeum.

Cellulose-binding domains (CBDs) play a vital role in cellulose degradation by enzymes. Despite the strong ability of brown-rot fungi to degrade cellulose in wood, they have been considered to lack or have a low number of enzymes with CBD. Here, we report the C-terminal domain of a lytic polysaccharide monooxygenase from the brown-rot fungus Gloeophyllum trabeum (GtLPMO9A-2) functions as a CBD, classified as a new family of carbohydrate-binding module, CBM104.