Immunochemical assessment of p16 and HPV L1 capsid protein in cervical squamous intraepithelial lesions.

Unlabelled: The behavior of the cervical squamous intraepithelial lesions cannot be predicted, many of them, particularly of the low grade type, may disappear without treatment. Invasive cervical carcinoma occurs in approximately 10% of the intraepithelial precursor lesions, being strongly associated with HPV infection. The aim of this study was to make a comparative assessment between immunohistochemical and immunocytochemical expression of p16 and L1 HPV capsid protein respectively, in low grade and high grade cervical squamous intraepithelial lesions.

Hereditary hemochromatosis in north-eastern Romania.

Unlabelled: Hereditary hemochromatosis is a genetic disturbance of iron metabolism resulting in iron overload in several organs and their functional failure. Early diagnosis is necessary to start a simple and effective therapy: bleeding. It is the most frequent genetic disease in some populations.

Therapeutic approaches in hereditary angioedema.

Hereditary angioedema (HAE) is characterized by acute attacks of edema with multiple localizations, the laryngeal angioedema being the most potentially lethal. In HAE, C1-INH impairments cause episodic increase in kallikrein activity leading to attacks of angioedema. Several therapies have recently become available to treat or to prevent HAE attacks, and others are under evaluation for this indication.

Differential equations with small parameter with applications in radioimmunotherapy.

The Akzo Nobel research laboratories formulated this problem in their study of the penetration of radio-labeled antibodies into a tissue that has been infected by a tumor. This study was carried out for diagnostic as well as therapeutic purposes. The treatment of malignant diseases, after its primary treatment by surgery, is either by external beam radiotherapy, which is effective but local, or by chemotherapy, which is effective but not selective.

An alternative method for the removal of the ocular artifact from the EEG signal.

Unlabelled: The aim of this paper is to develop a technique of rejection or minimization of ocular artifacts from the electroencephalographic (EEG) recordings.

Material And Method: The method presented is based on mathematical morphology. The algorithm and the individual structuring element are presented along with the particularities concerning the parameters that characterize the structuring element.

Community pharmacy--ideal location to screen and monitor hypertensive patients.

Unlabelled: The goals of this study were (a) to create a patient electronic database by filling in the specially designed Patient Data Sheets (PDS) after interviewing hypertensive patients and (b) to evaluate pharmacists' opportunities for intervention in order to improve hypertensive patients' outcomes.

Material And Method: Pharmacists from two Romanian chain community pharmacies interviewed all hypertensive patients that entered the pharmacies during the study period (November-December 2008) and selected 106 subjects using several selection criteria. Pharmacists recorded in specially designed Patient Data Sheet relevant information such as: demographic data, additional diagnostics (if any), lifestyle behaviors and therapeutic data.

Evaluation of some pharmaceutical formulations of lisinopril through dissolution testing.

Aim: Lisinopril is a drug of the angiotensin converting enzyme (ACE) inhibitor class that is primarily used in treatment of hypertension, congestive heart failure, heart attacks and also in preventing renal and retinal complications of diabetes. We compared the dissolution profiles of Lisinopril 20 mg tablets (Antibiotice S.A.

Measurement of total antioxidant activity with chlorpromazine radical cation.

Unlabelled: In the present study there is described a simple and sensitive method for the evaluation of antioxidant activity in chlorpromazine-Fe (III) system using ascorbic acid as standard.

Material And Method: Chlorpromazine [2-chloro-N-(3-dimethyl-aminopropyl)-phenothiazine] is oxidized in acidic media by Fe (III) with the formation of a stable radical cation characterized by an intense visible absorbtion band (lambda = 540 nm). The optimum parameters for the stability of the radical cation have been studied: molar ratio chlorpromazine-Fe (III), pH, solvents.

Grapefruit juice--drug interactions: importance for pharmacotherapy.

In spite of known health benefits of grapefruit juice, its consumption in combination with drugs requires caution. The drugs most susceptible to pharmacokinetic interactions with clinical significance are those with narrow therapeutic index and low bioavailability due to important first-pass metabolism. Most vulnerable populations are elderly, cirrhotics, subjects with genetic polymorphisms and individuals taking other CYP3A4 inhibitors.

Ghrelin and Ang 1-7 have cumulative vasodilatory effects on pulmonary vessels.

Unlabelled: Ghrelin is an orexigenic hormone that has been shown to have vasodilator effects. Angiotensin 1-7 (Ang 1-7) is a bioactive component of the renin-angiotensin system and may play an important role in the regulation of cardiovascular homeostasis.

Aim: The aim of this study was to investigate the interaction between ghrelin and a better known vasodilatator, Ang 1-7.

Early recurrence in favorable stage II breast cancer--which approach is the best?

Aim: Changing the sequence of therapeutic options in stage II breast cancer: first, a core biopsy, followed by the evaluation of the tumoral markers, adaptation of the chemotherapy scheme and finally, surgical approach. Thus would be possible to improve the hope of life in some stage II breast cancer patients, in whom survival is poorer than in some stage III patients.

Material And Method: 144 patients in stage II breast cancer were included in this study, over a period of 5 years (2000-2004).

New original in vitro method to assess cholinesterase reactivity in organophosphate poisoning.

Assessment of organophosphate poisoning could benefit from a safe, non-expensive, easy to perform, quick (< 1 hour) test, which evaluates the level of cholinesterase activity "in vitro" regarding to the capability of oximes to reactivate OF-blocked cholinesterase. In the proposed protocol, 0.5 mL of sample serum is incubated, prior to the evaluation of level of cholinesterase activity, with 5 microL of a Toxogonin dilution (0.

Differentiation of patients with chronic lombosciatica due to disc hernia using gait analysis techniques.

Unlabelled: Nowadays gait analysis represent a modern technique used as a useful tool in the assessment of patients with locomotors disease.

Aim: To make a differentiation of patients with chronic sciatica due to disc hernia using gait analysis techniques.

Material And Method: Study was prospective, with consecutive selection of subjects according to eligibility criteria, using a control group.

Nanoparticles biodistribution in the eye.

Nowadays the management of ocular diseases is limited by the inability for controlled drug delivery. In the present study micro and nanoparticles based on Chitosan labeled with fluorescein, prepared by double crosslinking method in water/oil emulsion, were investigated as a new vehicle for intraocular drug delivery. The main objective was to study the biodistribution of the prepared particles after beind administered into the anterior chamber of pig eyes.

Pitrakinra for asthma.

Importance Of The Field: In asthma IL-4 and IL-13 have been demonstrated to play major pathogenic roles and therefore their blockade would potentially represent a plausible therapeutic approach.

Areas Covered In This Review: Pitrakinra is a dual IL-4/IL-13 inhibitor currently under development for asthma and the existing preclinical and clinical data are discussed.

What The Reader Will Gain: Inhaled pitrakinra demonstrated a good anti-inflammatory potential and a good safety profile on a short-term basis but its place in asthma therapy is still to be found.

Daclizumab: a potential asthma therapy?

Airways inflammation in asthma is triggered and maintained by CD4+ (Th2) cells which are activated by IL-2 and stimulate the eosinophilic inflammation, IgE secretion and mucus hyperproduction. Current anti-inflammatory therapies include inhaled corticosteroids, and leukotriene modifiers but they are not universally effective. IL-2 pathway inhibition might represent a potent anti-inflammatory therapy in asthma given the cytokine early role in the asthma complex inflammatory cascade and daclizumab which is a IL-2R blocker currently used as an immunsupressor in organ transplantation might be a potential asthma therapy.

Socioeconomic implications of underdiagnosing teenagers' depression.

Unlabelled: The aim of our research was to demonstrate once again that teenagers' depression still remains way too much underdiagnosed, the last one having important socioeconomic implications. Thus it is absolutely necessary to implement depression screening in psychiatric clinics.

Material And Method: The study was carried out in 2 stages, I; a group of 1126 in-patients from "Sf.

The pharmacological activity of some new fluorescent small molecule histamine H2 receptor (H2-R) ligands, derived from aminopotentidine and squaramide, in the GTPase assay.

Unlabelled: We characterized the pharmacological activity of some new fluorescent and nonfluorescent ligands derived from aminopotentidine and squaramide with respect to the interaction with human (h) and guinea pig (gp) H2R species isoforms on Spodoptera frugiperda (Sf9) cell membranes expressing H2R-Gsalpha fusion proteins.

Material And Method: The pharmacological studies were performed in a radioactivity-based GTPase assay. Non-linear curve ?tting was performed using the computer program Prism 4.

Researches of the nitrite/nitrate presence and other chemical compounds in some water sources from rural area of Neamţ county.

Unlabelled: Water is one of the main dietary components and his quality may be influenced the health status of the population.

Material And Methods: We investigated the water quality (by ten chemical parameters) delivered to the rural population (Neamţ district), from the public water supply systems (n=10) with ground water sources and 60 well waters in same area. For determinations of the investigated chemical compounds, spectrophotometric, complexonometric and volumetric methods were performed.

Laryngotracheal non-Hodgkin's lymphoma.

Objectives: Malignant lymphomas of larynx and trachea are rare tumors and require special diagnostic and therapeutic attention. The authors present an unexpected case of nonHodgkin Lymphoma localized in the subglottic larynx and upper cervical trachea. The clinical presentation, diagnostic and therapeutic approach, evolution and prognosis are discussed.

Effects of inhaled therapies on health-related quality of life in stable chronic obstructive pulmonary disease.

In chronic obstructive pulmonary disease (COPD) health-related quality-of-life (HRQoL)/health status improvement is included among the management goals. This can be achieved, among other measures, with inhaled therapies, which, in this disease, are used alone or combined in a step-up approach based on disease severity. As COPD is a chronic disease, the long-term effects of inhaled therapies on various disease outcome measures, such as symptoms, lung function, morbidity or HRQoL, are more relevant for accessing clinical efficacy.

Adherence to inhaled therapy in COPD: effects on survival and exacerbations.

Evaluation of: Vestbo J, Anderson JA, Calverley PMA et al. Adherence to inhaled therapy, mortality and hospital admission in COPD. Thorax 64, 939-943 (2009).

Effects of dietary flaxseed supplementation on renal oxidative stress in experimental diabetes.

Unlabelled: Previous results demonstrate that experimental diabetes mellitus is accompanied by increased oxidant stress within glomeruli. Evidence are emerging that dietary flaxseed supplementation can have beneficial effects on oxidative stress.

Materials And Methods: Diabetes was induced by intraperitoneal injection of streptozotocin (STZ) in male Golden Syrian hamsters, and both diabetic and control groups were fed either standard diet, or standard diet supplemented with flaxseed (15 g/100 g diet), for 20 weeks.

Biogenic amines determination in some Romanian meat products.

Aim: Biogenic amines are compounds synthesized in human body which in high concentration become toxic and lead to a wide range of symptoms as palpitations, nausea and headache. Meat products can contain high levels of biogenic amines. Ingestion of such product can cause severe illness.

Using MLPA for large genomic rearrangements detection in breast cancer predisposition genes.

Unlabelled: Breast and ovarian cancer are common pathologies in women, with increasing incidences worldwide. In hereditary breast and ovarian cancer (HBOC) families, a large percentage of cases are attributable to hereditary factors compatibles with dominant autosomal transmission of a major tumour suppressor gene with incomplete penetrance. Screening for BRCA1 mutations is now standard practice for HBOC cases in western world, and permits medical follow-up and genetic counselling.