Accelerometry: a technique for quantifying movement patterns during walking.

The popularity of using accelerometer-based systems to quantify human movement patterns has increased in recent years for clinicians and researchers alike. The benefits of using accelerometers compared to more traditional gait analysis instruments include low cost; testing is not restricted to a laboratory environment; accelerometers are small, therefore walking is relatively unrestricted; and direct measurement of 3D accelerations eliminate errors associated with differentiating displacement and velocity data. However, accelerometry is not without its disadvantages, an issue which is scarcely reported in gait analysis literature.

Strength training improves supramaximal cycling but not anaerobic capacity.

This study examined supramaximal cycling performed to exhaustion at 120% of peak O(2) uptake (120% VO(2)peak) before and after 8 weeks of strength training. Eight previously untrained men completed 8 weeks of leg-strength training 3 days week(-1) on a hack-squat machine; four sets, five repetitions at 85% of one repetition maximum each session. Anaerobic capacity was quantified by determining the maximal accumulated O(2) deficit during supramaximal cycling.

Threat interpretation bias as a vulnerability factor in childhood anxiety disorders.

The present study examined threat interpretation biases in children 7-12 years of age with separation, social and generalised anxiety disorders (N=15), non-anxious offspring at risk due to parental anxiety (N=16) and non-anxious controls of non-anxious parents (N=14). Children provided interpretations of ambiguous situations to assess cognitive, emotional and behavioural responses. In comparison with non-anxious control children and at-risk children who did not differ from each other, anxious children reported stronger negative emotion and less ability to influence ambiguous situations.

Evaluating a Web-enhanced bachelor of nursing curriculum: perspectives of third-year students.

Little is known about nursing students' information literacy skills and perceptions of Web-enhanced educational approaches. This study examined graduating Bachelor of Nursing (BN) students' perceptions of a Web-enhanced learning environment, their computer literacy skills, and use of technology, and how these influenced their satisfaction. This Australian survey produced a 64% (n = 170) response rate.

Latitudinal variation in the prevalence and intensity of chytrid (Batrachochytrium dendrobatidis) infection in eastern Australia.

Chytridiomycosis is a recently emerged, infectious skin disease of amphibians that has been linked directly to mass mortalities, population declines, and species extinctions worldwide. An understanding of the factors that limit the distribution and abundance of Batrachochytrium dendrobatidis (the etiological agent of chytridiomycosis) is urgently required. We conducted disease surveys at 31 lowland sites distributed north-south along 2315 km of the Australian east coast that encompassed 20.

Energy turnover for Ca2+ cycling in skeletal muscle.

The majority of energy consumed by contracting muscle can be accounted for by two ATP-dependent processes, cross-bridge cycling and Ca(2+) cycling. The energy for Ca(2+) cycling is necessary for contraction but is an overhead cost, energy that cannot be converted into mechanical work. Measurement of the energy used for Ca(2+) cycling also provides a means of determining the total Ca(2+) released from the sarcoplasmic reticulum into the sarcoplasm during a contraction.

Assessing relationships among properties of demolished concrete, recycled aggregate and recycled aggregate concrete using regression analysis.

Recycled demolished concrete (DC) as recycled aggregate (RA) and recycled aggregate concrete (RAC) is generally suitable for most construction applications. Low-grade applications, including sub-base and roadwork, have been implemented in many countries; however, higher-grade activities are rarely considered. This paper examines relationships among DC characteristics, properties of their RA and strength of their RAC using regression analysis.

Matrix metalloproteinase localisation by in situ-RT-PCR in archival human breast biopsy material.

Utilising archival human breast cancer biopsy material we examined the stromal/epithelial interactions of several matrix metalloproteinases (MMPs) using in situ-RT-PCR (IS-RT-PCR). In breast cancer, the stromal/epithelial interactions that occur, and the site of production of these proteases, are central to understanding their role in invasive and metastatic processes. We examined MT1-MMP (MMP-14, membrane type-1-MMP), MMP-1 (interstitial collagenase) and MMP-3 (stromelysin-1) for their localisation profile in progressive breast cancer biopsy material (poorly differentiated invasive breast carcinoma (PDIBC), invasive breast carcinomas (IBC) and lymph node metastases (LNM)).

The rainbow trout CMP-sialic acid synthetase utilises a nuclear localization signal different from that identified in the mouse enzyme.

The terminal sugar sialic acid (Sia) plays a pivotal role in cell-cell interaction and recognition. A prerequisite for the biosynthesis of sialoglycoconjugates is the activation of Sia to cytidine monophosphate-Sia (CMP-Sia), by CMP-Sia synthetases (CMP-Sia-syn). CMP-Sia-syn are conserved from bacteria to man, and have been found to reside in the nucleus of all vertebrate species analysed to date.

On the effectiveness in implementing a waste-management-plan method in construction.

The increasing awareness of waste management concerns from construction and demolition waste has led to the development of waste management as an important function of construction project management. The Hong Kong government started employing the implementation of a waste-management-plan (WMP) method for all construction projects in 2003. During the trial period, the government received different version of feedback from the industry.

Direct detection of ligand binding to Sepharose-immobilised protein using saturation transfer double difference (STDD) NMR spectroscopy.

We report an easy and direct application of 'Saturation Transfer Double Difference' (STDD) NMR spectroscopy to identify ligands that bind to a Sepharose-immobilised target protein. The model protein, cytidine 5'-monophosphate sialic acid (CMP-Sia) synthetase, was expressed as a Strep-Tag II fusion protein and immobilised on Strep-Tactin Sepharose. STD NMR experiments of the protein-enriched Sepharose matrix in the presence of a binding ligand (cytidine 5'-triphosphate, CTP) and a non-binding ligand (alpha/beta-glucose) clearly show that CTP binds to the immobilised enzyme, whereas glucose has no affinity.

Simple, novel physical activity maintains proximal femur bone mineral density, and improves muscle strength and balance in sedentary, postmenopausal Caucasian women.

Unlabelled: A simple, appealing, physical activity program can be prescribed to reduce the risk of falls in sedentary, postmenopausal, independent-living, Caucasian women. Foot stamping, progressively loaded squats, and in-line dancing positively influence proximal femoral bone mineral density, lower extremity strength, and static and dynamic balance.

Introduction: Foot stamping, squats exercises, and in-line dancing together create a suitable activity program for sedentary, independent-living older women.

The effect of manipulating sediment pH on the porewater chemistry of copper- and zinc-spiked sediments.

Spiking of sediment with metal cations that readily hydrolyse causes the sediment pH to decrease. Displaced iron and manganese also oxidise and hydrolyse, further lowering sediment pH. The lower pH of metal-spiked sediments requires a subsequent sediment neutralisation.

Synthesis and biological evaluation of galactofuranosyl alkyl thioglycosides as inhibitors of mycobacteria.

As part of our research interest directed toward the development of antimycobacterial agents, we have investigated compounds based on galactofuranose (Galf), an essential cell wall component of mycobacteria. The objective of this study was to explore structure activity relationships of Galf thioglycosides with straight chain and branched aglycons. Acylated Galf 9-heptadecyl thioglycoside was prepared by Lewis acid-catalyzed thioglycosidation of 1,2,3,5,6-penta-O-acyl-D-galactofuranose with 9-heptadecanethiol, and subsequently converted to the corresponding sulfone using m-CPBA.

The mechanics of mouse skeletal muscle when shortening during relaxation.

The dynamic properties of relaxing skeletal muscle have not been well characterised but are important for understanding muscle function during terrestrial locomotion, during which a considerable fraction of muscle work output can be produced during relaxation. The purpose of this study was to characterise the force-velocity properties of mouse skeletal muscle during relaxation. Experiments were performed in vitro (21 degrees C) using bundles of fibres from mouse soleus and EDL muscles.

A fast stir bar sorptive extraction method for the analysis of geosmin and 2-methylisoborneol in source and drinking water.

The presence of unpleasant taste and odour in drinking water is an ongoing aesthetic concern for water providers worldwide. The need for a sensitive and robust method capable of analysis in both natural and treated waters is essential for early detection of taste and odour events. The purpose of this study was to develop and optimise a fast stir bar sorptive extraction (SBSE) method for the analysis of geosmin and 2-methylisoborneol (MIB) in both natural water and drinking water.

Novel cationic lipophilic peptides for oligodeoxynucleotide delivery.

In search of new oligodeoxynucleotide (ODN) delivery agents, we evaluated novel peptides derived from core peptide H-GLRILLLKV-OH (CP). CP is a fragment designed from the T-cell antigen receptor (TCR) alpha-chain transmembrane sequence. CP was able to enter cells including T-cells and inhibited interleukin-2 (IL-2) production.

Inverse relations in the patterns of muscle and center of pressure dynamics during standing still and movement postures.

The aim of this study was to investigate the postural center of pressure (COP) and surface muscle (EMG) dynamics of young adult participants under conditions where they were required to voluntarily produce random and regular sway motions in contrast to that of standing still. Frequency, amplitude and regularity measures of the COP excursion and EMG activity were assessed, as were measures of the coupling relations between the COP and EMG outputs. The results demonstrated that, even when standing still, there was a high degree of regularity in the COP output, with little difference in the modal frequency dynamics between standing still and preferred motion.

Detection of intermittent sewage pollution in a subtropical, oligotrophic, semi-enclosed embayment system using sterol signatures in sediments.

A field study was conducted to investigate sewage inputs at popular anchorages in Moreton Bay, a sub-tropical, semienclosed embayment system in Southeast Queensland, Australia. Sterol biomarkers were quantified in sediments revealing low levels over a spatial and temporal scale consistent with a shallow, oligotrophic, highly dynamic, sand dominated system. Despite low concentrations (ng/g) and high variability, relevant sterol/stanol pairs remained well-correlated and were successful in identifying an unexpected once-off pollution event from a point source at Moreton Bay Island.

Slow diffusion of lactose out of galectin-3 crystals monitored by X-ray crystallography: possible implications for ligand-exchange protocols.

Galectin-3 is a multifunctional carbohydrate-binding protein that has roles in cancer progression. In addition to carbohydrate-dependent extracellular functions, galectin-3 participates in carbohydrate-independent intracellular signalling pathways, including apoptosis, via protein-protein interactions, some of which engage the carbohydrate-binding groove. When ligands bind within this site, conformational rearrangements are induced and information on unliganded galectin-3 is therefore valuable for structure-based drug design.

Insight into host cell carbohydrate-recognition by human and porcine rotavirus from crystal structures of the virion spike associated carbohydrate-binding domain (VP8*).

Rotavirus infection leads to the death of half a million children annually. The exact specifics of interaction between rotavirus particles and host cells enabling invasion and infection have remained elusive. Host cell oligosaccharides are critical components, and their involvement aids the virus in cell-recognition and attachment, as well as dictation of the remarkable host-specificity that rotaviruses demonstrate.

Verbal numerical scales are as reliable and sensitive as visual analog scales for rating dyspnea in young and older subjects.

This study compared the use of a simple verbal 0-10 numerical rating scale (verbal NRS) and a visual analog scale (VAS) for the rating of dyspnea during exercise in a group of young and older subjects. Twelve younger (32+/-9 yr) and 12 older (71+/-7 yr) subjects used either the verbal NRS or the VAS in a randomised fashion to rate dyspnea during 60 s of uphill treadmill walking (range 5.6-8.

Synthesis and evaluation of galactofuranosyl N,N-dialkyl sulfenamides and sulfonamides as antimycobacterial agents.

The recent emergence of clinically oppressive superbugs, some with resistance to nearly all frontline drug therapies, has challenged our ability to combat such infectious organisms as Mycobacterium tuberculosis, the causative agent of tuberculosis (TB). Our medicinal chemistry program targeting this pathogen has identified several potent galactofuranose-based in vitro inhibitors of mycobacterial growth. The most potent compound, the Galf N,N-didecyl sulfenamide 8d, displayed anti-mycobacterial activity (MIC) of 1 microg/mL in a cell based assay against a representative strain of Mycobacterium smegmatis.