Effects of exogenous 17β-estradiol on follicular development in the neonatal and immature mouse in vivo.

Purpose: The study was aimed to examine the effects of exogenous 17β-estradiol (E) on oocyte nest breakdown and follicular growth in the ovary of neonatal and immature mice.

Methods: The effect of E on primordial follicle formation and differentiation were examined by treating neonatal mice with E in mineral oil at a dose of 10 mg/kg by sc injection each day from postnatal days 1 (PND1) to 10 (PND10). This investigation was conducted by using histological observation and immunohistochemical staining of proliferating cell nuclear antigen (PCNA); the numbers of follicles at each stage were examined to assess the follicular development.

Genetic characterization of rabies field isolates from Thailand.

We sequenced 512 nucleotides in two variable regions of the N gene of 23 rabies isolates from the northeastern part of Thailand by direct sequencing of PCR-amplified products. The sequencing data revealed two new lineages in these rabies isolates. Based on the results of this study together with the findings of our earlier study, the rabies isolates in Thailand were divided into two genogroups, designated as T1 and T2, which were predominantly localized in the northern and northeastern areas, respectively.