[Bacterial contamination of infant urine samples obtained from filter papers used in neuroblastoma-screening tests. (6) Protective effects of preaddition of chlorhexidine digluconate in filter papers or containers for urine on bacterial break-down of creatinine, vanillylmandelic acid and homovanillic acid].

The effects of chlorhexidine digluconate preadded to urine samples for a neuroblastoma-screening test in preventing the break-down of creatinine (Cre), vanillylmandelic acid (VMA), and homovanillic acid (HVA) by creatinine-cleaving bacteria and the influence of the added disinfectant on the HPLC determination of urinary Cre, VMA, vanillillactic acid (VLA) and HVA. Laboratory or field experiments showed that preadded disinfectant (0.02% volume) nearly completely inhibited the growth of the bacteria and satisfactorily protected urinary Cre, VMA, and HVA from bacterial decomposition.

[An improved differential medium, CA medium, for differentiating Shigella].

We devised a Citrate-Acetate (CA) medium for rapidly differentiating Shigella. The medium consisted of 3.0 g of sodium citrate, 2.

[Epidemic of aseptic meningitis with echovirus type 18 in Gifu Prefecture in 1988].

On one hundred thirty seven cases with aseptic meningitis and 36 cases except aseptic meningitis in 1988, virological and serological investigations were performed. Cases with aseptic meningitis ranged from 0 to 15 years old age, and 90.5% of them were under 8 years of age.

[Epidemic of infectious disease with echovirus type 16--epidemic in Tono area, Gifu Prefecture in 1984].

During the period from May to August, 1984, an epidemic of infectious disease with echovirus type 16 occurred in Tono area of southeast in Gifu prefecture. This virus caused children to have different clinical symptoms, one was a exanthem disease and another was aseptic meningitis. These cases confirmed by virological and serological methods were 48 cases, that is, patients with aseptic meningitis were 24 cases and patients with exanthem disease were 24 cases.

[Diversity of etiological agent associated with aseptic meningitis--a survey on an epidemic in Tajimi City, Gifu Prefecture in 1984].

During the period from May to September in 1984, an epidemic of aseptic meningitis occurred in Tajimi city, Gifu prefecture. Virological and serological investigations were performed. Cases involved ranged from 0 to 12 years of age, and 83.

Serotype analysis of group A human rotavirus related to acute gastroenteritis in winter in Gifu city.

A total of 348 fecal specimens collected from children with acute gastroenteritis in Gifu city over three consecutive winter seasons (from November 1986 to March 1989) were examined for group A human rotavirus (HRV) by either a commercial test kit or a sandwich enzyme-linked immunosorbent assay (sandwich ELISA). One hundred twelve of the 173 group A HRV-positive specimens were further subjected to serotype determination by ELISA with four serotype-specific monoclonal antibodies to VP7 (ELISA-serotyping). Ninety-one specimens (81.