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Chemical labeling of intracellular proteins via affinity conjugation and strain-promoted cycloadditions in live cells.

Chem Commun (Camb)

November 2015

Chemical Genomics Centre of the Max Planck Society, Otto-Hahn-Str. 15, 44227 Dortmund, Germany. and Max-Planck Institute for Molecular Physiology, Otto-Hahn-Str. 11, 44227 Dortmund, Germany.

A versatile chemical labeling approach was developed, where intracellular proteins were first incorporated with a bioorthogonal group via affinity conjugation, and subsequently labeled via strain-promoted cycloaddition reactions in live cells.

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