Nf-Root: A Best-Practice Pipeline for Deep-Learning-Based Analysis of Apoplastic pH in Microscopy Images of Developmental Zones in Plant Root Tissue.

Hormonal mechanisms associated with cell elongation play a vital role in the development and growth of plants. Here, we report Nextflow-root (nf-root), a novel best-practice pipeline for deep-learning-based analysis of fluorescence microscopy images of plant root tissue from A. thaliana.

1-[(2-Chloro-phen-yl)di-phenyl-meth-yl]-1-pyrazole.

The title compound CHClN, also named as TRAM-34, crystallizes in the monoclinic space group 2/n. The dihedral angles between the pyrazole ring and the three six-membered rings are 62.28 (9), 69.

-2-{3-[1-(Acet-yloxy)eth-yl]-2,2-di-methyl-cyclobut-yl}acetic acid.

The title compound, CHO, was prepared from α-pinene in three steps. The ester and acid moieties are on the slightly folded cyclo-butane ring. In the crystal, carb-oxy-lic acid bound dimers form layers parallel to (202).

-Bis(quinoline-8-amine-κ ,')bis-(1,1,3,3-tetra-cyano-2-meth-oxy-propenido-κ)iron(II).

The title compound, [Fe(CHNO)(CHN)], was synthesized solvothermally. The complex exhibits a distorted octa-hedral coordination geometry. The Fe ion is located on an inversion centre.

The attraction of evil. An investigation of factors explaining women's romantic parasocial relationships with bad guys in movies and series.

The attractiveness of bad boys can be seen as a cultural phenomenon that can be found in different areas of society and art. In the media, too, the bad boy fulfills social expectations in terms of masculinity and is often portrayed as dominant, violent, hard, unemotional and aggressive. Women may feel attracted to this male dominance under certain conditions.

Synthesizing and assessing influenza vaccine evidence: Strengths and limitations of the recent ECDC report on the effectiveness of new and enhanced influenza vaccines. Communication on: the "European Centre for Disease Prevention and Control. Systematic review update on the efficacy, effectiveness and safety of newer and enhanced seasonal influenza vaccines for the prevention of laboratory confirmed influenza in individuals aged 18 years and over. Stockholm: ECDC; 2024".

High quality research is critical for evidence-based decision making in public health and fundamental to maintain progress and trust in immunization programs in Europe. In 2024 the European Centre for Disease Prevention and Control (ECDC) conducted an update of the 2020 systematic review to capture more recent evidence on of the efficacy, effectiveness of influenza vaccines in individuals aged 18 years and older in the prevention of laboratory-confirmed influenza. While this report was highly anticipated due to the strength of the protocol and processes put in place, during our assessment, we expressed two chief concerns.

EBV-specific T-cell immunity: relevance for multiple sclerosis.

Genetic and environmental factors jointly determine the susceptibility to develop multiple sclerosis (MS). Improvements in the design of epidemiological studies have helped to identify consistent environmental risk associations such as the increased susceptibility for MS following Epstein-Barr virus (EBV) infection, while biological mechanisms that drive the association between EBV and MS remain incompletely understood. An increased and broadened repertoire of antibody and T-cell immune responses to EBV-encoded antigens, especially to the dominant CD4 T-cell EBV nuclear antigen 1 (EBNA1), is consistently observed in patients with MS, indicating that protective EBV-specific immune responses are deregulated in MS and potentially contribute to disease development.

Generation of effective and specific human TCRs against tumor/testis antigen NY-ESO-1 in mice with humanized T cell recognition system.

Generation of high avidity T cell receptors (TCRs) reactive to tumor-associated antigens (TAA) is impaired by tolerance mechanisms, which is an obstacle to effective T cell therapies for cancer treatment. NY-ESO-1, a human cancer-testis antigen, represents an attractive target for such therapies due to its broad expression in different cancer types and the restricted expression in normal tissues. Utilizing transgenic mice with a diverse human TCR repertoire, we isolated effective TCRs against NY-ESO-1 restricted to HLA-A*02:01.

PGM3 insufficiency: a glycosylation disorder causing a notable T cell defect.

Background: Hypomorphic mutations in the () gene cause a glycosylation disorder that leads to immunodeficiency. It is often associated with recurrent infections and atopy. The exact etiology of this condition remains unclear.

Characteristics of patients with somatoform pain disorder.

Background: Patients with somatoform pain experience physical pain that cannot be attributed to any underlying medical or physiological cause, and it is often thought to be related to psychological factors. Health professionals encounter difficulties identifying this specific type of chronic pain, leading to suboptimal treatment strategies. Therefore, we aimed to describe the characteristics of patients with somatoform pain, to support the identification of affected patients.

Mapping the reporting practices in recent randomised controlled trials published in : A scoping review of methodological quality.

Unlabelled: The official medical journals of scientific societies advocate for high-quality standards. It's important to assess whether randomized controlled trials (RCTs) in influential journals, such as the hybrid journal of the European Society of , and (), adhere to reporting guidelines and best practices. Therefore, the present scoping review aimed to explore and map the reporting practices and methodological quality in recent RCTs published in the () journal, focusing on identifying gaps in adherence to reporting guidelines and transparency.

C-X-C motif chemokine receptor 4-directed PET signal in the arterial tree is not consistently linked to calcified plaque burden and cardiovascular risk.

To establish the extent, distribution and frequency of in-vivo vessel wall [Ga]Ga-PentixaFor uptake and to determine its relationship with calcified atherosclerotic plaque burden (CAP) and cardiovascular risk factors (CVRF). 65 oncological patients undergoing [Ga]Ga-PentixaFor PET/CT were assessed. Radiotracer uptake (target-to-background ratio [TBR]) and CAP burden (including number of CAP sites, calcification circumference and thickness) in seven major vessel segments per patient were determined.

Management of an Intraparenchymal Struvite Stone Using Laparoscopic Nephrolithotomy: A Case Report.

This study aims to present a case of laparoscopic nephrolithotomy and highlight its successful outcome. The patient was a 65-year-old male who experienced intermittent colicky flank pain. Imaging revealed the presence of a 20-mm round-shaped stone in a hydronephrotic calyx located in the mid-pole of the left kidney, possibly with a parenchymal component.

First isolation of from clinical specimens collected on a pig farm in Poland.

Introduction: Successful retrieval of from porcine clinical specimens has been rarely described, and data has only been obtained from a few swine-producing countries. Therefore, the aim of this study was the isolation of recovered from a specimen originating from a commercial pig farm located in Poland.

Material And Methods: Seven dead 12-week-old pigs weighing 24-26 kg with joint swelling of the hind legs were selected on a modern farrow-to-nursery farm in Poland in October 2023.

Lumbar spinal stenosis and surgical decompression affect sleep quality and position in patients. A prospective cross-sectional cohort study.

Introduction: It is reasonable to assume that lumbar spinal stenosis (LSS) affects the cauda nerve roots also at night.

Research Question: Does microsurgical decompression influence sleep quality and position?

Materials And Methods: A study nurse interviewed 140 patients scheduled for LSS decompression using the Pittsburgh Sleep Quality Index (PSQI), Spinal Stenosis Measure (SSM), Numeric Rating Scale (NRS) for back and leg pain, Douleur Neuropathique (DN4), and Charlson Comorbidity Index. Epidemiologic and MRI data were collected along with self-reported rankings of preferred sleep positions (prone, supine, side, and fetal).

Determining the role of basophil activation testing in reported type 1 allergy to beta-lactam antibiotics.

Background: Allergy to beta-lactam antibiotics (BLA), especially to penicillin, is the most commonly reported drug allergy by patients. Alternative antibiotics can yield negative consequences, such as extended hospitalization days due to less efficacy and overall higher costs. The basophil activation test (BAT) is an assay, in which activation of an individual's own basophils is quantified by flow cytometry.

Synthesis, crystal structure and properties of -poly[[bis-(4-methyl-pyridine-κ)cobalt(II)]-di-μ-thio-cyanato-κ :;κ :], which shows a rare coordination geometry.

Reaction of Co(NCS) with 4-methyl-pyridine in water leads to the formation of single crystals of the title compound, [Co(NCS)(CHN)] . The asymmetric unit consists of two crystallographically independent thio-cyanate anions and two crystallographically independent 4-methyl-pyridine coligands in general positions, as well as of two different Co cations, of which one is located on a twofold rotational axis, whereas the second occupies a center of inversion. The methyl H atoms in both 4-methyl-pyridine ligands are disordered and were refined using a split model.

Synthesis, crystal structure and thermal properties of poly[di-μ-bromido-(μ-2,5-di-methyl-pyrazine)cadmium(II)].

The title compound, [CdBr(CHN)] , was prepared by the reaction of cadmium bromide with 2,5-di-methyl-pyrazine in water. Its asymmetric unit consists of one Cd cation and one 2,5-di-methyl-pyrazine ligand that are located on a crystallographic mirror plane as well as one bromide anion that occupies a general position. The Cd cations are sixfold coordinated by four bromide anions and two 2,5-di-methyl-pyrazine ligands within slightly distorted -CdBrN octa-hedra.

Deep-learning-based image compression for microscopy images: An empirical study.

With the fast development of modern microscopes and bioimaging techniques, an unprecedentedly large amount of imaging data is being generated, stored, analyzed, and shared through networks. The size of the data poses great challenges for current data infrastructure. One common way to reduce the data size is by image compression.

Jaak Panksepp's primary emotions are associated with Diener's global life satisfaction: How low arousal of the SADNESS/separation distress system could form the core of life satisfaction?

We compared Ed Diener's (SWLS), which was designed as a purely cognitive measure of global life satisfaction, with the , which provides self-report measures of Panksepp's six primary emotions (excluding LUST), in two English-speaking samples: a main sample and a hold-out validation sample. Our data showed robust negative correlations between higher satisfaction with life and lower FEAR, lower SADNESS/Separation Distress, and positive associations (albeit less strong) between higher satisfaction with life and higher PLAY and SEEKING in both samples. The relationships between the SWLS and at least four of Panksepp's primary emotions suggest Diener's SWLS is not purely cognitive and includes a strong affective component.

Harmonic weak Maass forms and periods II.

In this paper we investigate the Fourier coefficients of harmonic Maass forms of negative half-integral weight. We relate the algebraicity of these coefficients to the algebraicity of the coefficients of certain canonical meromorphic modular forms of positive even weight with poles at Heegner divisors. Moreover, we give an explicit formula for the coefficients of harmonic Maass forms in terms of periods of certain meromorphic modular forms with algebraic coefficients.

Role of the tumor board when prescribing mutant isocitrate dehydrogenase inhibitors to patients with isocitrate dehydrogenase-mutant glioma.

Isocitrate dehydrogenase (IDH)-mutant gliomas, comprising both astrocytomas and oligodendrogliomas, represent a distinct group of tumors that pose an interdisciplinary challenge. Addressing the needs of affected patients requires close collaboration among various disciplines, including neuropathology, neuroradiology, neurosurgery, radiation oncology, neurology, medical oncology, and other relevant specialties when necessary. Interdisciplinary tumor boards are central in determining the ideal diagnostic and therapeutic strategies for these patients.

Financial challenges of being on long-term, high-cost medications.

The isocitrate dehydrogenase (IDH) inhibitor, vorasidenib, may offer a promising new treatment option for patients with IDH-mutant gliomas. However, the indefinite nature of this targeted therapy raises significant financial concerns. High costs of targeted cancer therapies, often exceeding $150 000 annually, contribute to financial toxicity, characterized by medical debt, income loss, and psychological stress, and place stress on health systems.

Mapping the research landscape of microRNAs in pain: a comprehensive bibliometric analysis.

Background And Objectives: MicroRNAs (miRNAs) have demonstrated significant potential in pain medicine research, including mechanisms, diagnosis, and therapy. However, no relative bibliometric analysis has been performed to summarize the progress in this area quantitatively.

Methods: Literature was retrieved from the Web of Science Core Collection online database.