Polarity signaling balances epithelial contractility and mechanical resistance.

Epithelia maintain a functional barrier during tissue turnover while facing varying mechanical stress. This maintenance requires both dynamic cell rearrangements driven by actomyosin-linked intercellular adherens junctions and ability to adapt to and resist extrinsic mechanical forces enabled by keratin filament-linked desmosomes. How these two systems crosstalk to coordinate cellular movement and mechanical resilience is not known.

Proteinuric chronic kidney disease is associated with altered red blood cell lifespan, deformability and metabolism.

Anemia is a common complication of chronic kidney disease, affecting the quality of life of patients. Among various factors, such as iron and erythropoietin deficiency, reduced red blood cell (RBC) lifespan has been implicated in the pathogenesis of anemia. However, mechanistic data on in vivo RBC dysfunction in kidney disease are lacking.

Brain activation during the observation of real soccer game situations predicts creative goal scoring.

Creativity is an important source of success in soccer players. In order to be effective in soccer, unpredictable, sudden and at the same time creative (i.e.

Three-dimensional CoM energetics, pelvis and lower limbs joint kinematics of uphill treadmill running at high speed.

The purpose of this study was to investigate the effects of slope on three-dimensional running kinematics at high speed. Thirteen male sprinters ran at high speed (7.5 m/s) on a motorised treadmill in each a level and a 5.

Nestin progenitor cells isolated from adult human sweat gland stroma promote reepithelialisation and may stimulate angiogenesis in wounded human skin ex vivo.

The combination of an aging population and an increasing prevalence of diseases associated with impaired-wound healing, including obesity, peripheral vascular disease and diabetes, is likely to result in a dramatic increase in the incidence and prevalence of chronic skin wounds. Indeed, systemic reviews are now not only trying to establish both the prevalence and the often under-estimated socio-economic costs of chronic skin wounds, but most importantly are addressing the impact that chronic wounds have on quality of life. Given the clear need for novel approaches to the management of chronic skin ulceration, ideally developed and tested in the human system in a manner that can be rapidly translated into clinical practice, we examined the effects of multipotent primary human nestin progenitor cells on human wound healing in an ex vivo model.

Game-specific characteristics of sport-related concussions.

Background: Concussions are common incidences in sports. However, game-specific characteristics such as tactics, field positions, etc. might positively/negatively contribute to the occurrence of mild traumatic brain injuries (mTBI) in various sports such as soccer, volleyball, handball, or basketball.

Are Electrically Induced Muscle Cramps Able to Increase the Cramp Threshold Frequency, When Induced Once a Week?

The cramp threshold frequency (CTF) is known to be positively correlated with the individual cramp susceptibility. Here we assessed CTF changes after two bouts of electrically induced muscle cramps (EIMCs). The EIMCs (6×5 sec) were unilaterally induced twice (separated by one week) in the gastrocnemius of an intervention group (n=8), while 5 participants served as control.

Serum Cartilage Oligomeric Matrix Protein: is There a Repeated Bout Effect?

The primary aim of the present study was to investigate if there is a repeated bout effect for cartilage tissue, evident in the marker serum cartilage oligomeric matrix protein (sCOMP). Ten healthy male subjects (26.4±3.

Effect of exercise-induced enhancement of the leg-extensor muscle-tendon unit capacities on ambulatory mechanics and knee osteoarthritis markers in the elderly.

Objective: Leg-extensor muscle weakness could be a key component in knee joint degeneration in the elderly because it may result in altered muscular control during locomotion influencing the mechanical environment within the joint. This work aimed to examine whether an exercise-induced enhancement of the triceps surae (TS) and quadriceps femoris (QF) muscle-tendon unit (MTU) capacities would affect mechanical and biological markers for knee osteoarthritis in the elderly.

Methods: Twelve older women completed a 14-week TS and QF MTU exercise intervention, which had already been established as increasing muscle strength and tendon stiffness.

Influence of pole plant time on the performance of a special jump and plant exercise in the pole vault.

The purpose of this study was to examine the effect of the timing of the pole plant during the stance phase of the jump on the energy level of the vaulter/pole system at take-off for a special pole vault take-off exercise (Jagodin). We hypothesised that an earlier pole plant would increase the pole energy at take-off compared to the energy decrease of the vaulter during the jump and plant complex and so lead to a higher total energy of the vaulter/pole system at take-off. Six male pole vaulters experienced three Jagodins each with different pole plant time building three groups of vaults (early, intermediate, late pole plant).

Deficits in the way to achieve balance related to mechanisms of dynamic stability control in the elderly.

The purpose of this study was to examine the postural corrections related to components of dynamic stability aimed to increase our understanding of successful postural control among the elderly population. This was done by comparing balance behaviour of older adults who were able to recover stability (stable) and others who failed to regain stability (unstable) with a single step after a forward fall. Thirty-eight old male adults (64+/-3yr, 176+/-6cm, 78.

In vivo moment generation and architecture of the human plantar flexors after different shortening-stretch cycles velocities.

The purpose of this study was to examine the moment generation of the human plantar flexors and the architecture of the gastrocnemius medialis muscle during and after shortening-stretch cycles in vivo. Fourteen male subjects (30+/-7 years, 177+/-7 cm, 80+/-9 kg) performed a series of electro-stimulated shortening-stretch plantar flexion contractions. The shortening-stretch cycles were performed at three constant angular velocities (25 degrees /s, 50 degrees /s, 100 degrees /s), two amplitudes (15 degrees and 25 degrees ankle angle changes) and at two different stimulation frequencies (30 Hz and 85 Hz).

Adaptational responses of the human Achilles tendon by modulation of the applied cyclic strain magnitude.

Tendons are able to remodel their mechanical and morphological properties in response to mechanical loading. However, there is little information about the effects of controlled modulation in cyclic strain magnitude applied to the tendon on the adaptation of tendon's properties in vivo. The present study investigated whether the magnitude of the mechanical load induced as cyclic strain applied to the Achilles tendon may have a threshold in order to trigger adaptation effects on tendon mechanical and morphological properties.

Changes in fascicle length from rest to maximal voluntary contraction affect the assessment of voluntary activation.

The purpose of this study was to investigate the effect of the differences between the actual fascicle length during a voluntary contraction and the fascicle length at rest of the triceps surae muscle on the determination of the voluntary activation (VA) by using the interpolated twitch technique. Twelve participants performed isometric voluntary maximal (MVC) and submaximal (20%, 40%, 60% and 80% MVC) contractions at two different ankle angles (75 degrees and 90 degrees ) under application of the interpolated twitch technique. Two ultrasound probes were used to determine the fascicle length of soleus, gastrocnemius medialis and gastrocnemius lateralis muscles.

Age-related deficit in dynamic stability control after forward falls is affected by muscle strength and tendon stiffness.

The purpose of the work was to determine whether the age-related muscle weakness diminishes older adults' ability to use mechanisms responsible for maintaining dynamic stability after forward falls. Nine older and nine younger adults participated in this study. To analyse the capacities of the leg-extensor muscle-tendon units, all subjects performed isometric maximal voluntary plantarflexion and knee extension contractions on a dynamometer.

Muscle - tendon unit mechanical and morphological properties and sprint performance.

The objective of this study was to determine whether sprint performance is related to the mechanical (elongation - force relationship of the tendon and aponeurosis, muscle strength) and morphological (fascicle length, pennation angle, muscle thickness) properties of the quadriceps femoris and triceps surae muscle - tendon units. Two groups of sprinters (slow, n = 11; fast, n = 17) performed maximal isometric knee extension and plantar flexion contractions on a dynamometer at 11 different muscle - tendon unit lengths. Elongation of the tendon and aponeurosis of the gastrocnemius medialis and the vastus lateralis was measured using ultrasonography.

Effect of joint rotation correction when measuring elongation of the gastrocnemius medialis tendon and aponeurosis.

It is well known that during maximal plantar flexion contractions the ankle joint rotation overestimates the actual elongation of the tendon and aponeurosis. The aim of this study was to examine the influence of the curve length changes of the Achilles tendon on the joint rotation corrected elongation and strain of the gastrocnemius medialis (GM) tendon and aponeurosis. Nine subjects (age: 29.

Metabolism of 4-hydroxyandrostenedione and 4-hydroxytestosterone: Mass spectrometric identification of urinary metabolites.

4-Hydroxyandrost-4-ene-3,17-dione is a second generation, irreversible aromatase inhibitor and commonly used as anti breast cancer medication for postmenopausal women. 4-Hydroxytestosterone is advertised as anabolic steroid and does not have any therapeutic indication. Both substances are prohibited in sports by the World Anti-Doping Agency, and, due to a considerable increase of structurally related steroids with anabolic effects offered via the internet, the metabolism of two representative candidates was investigated.

Mechanical properties of the triceps surae tendon and aponeurosis in relation to intensity of sport activity.

The purpose of the present study was to investigate whether the mechanical properties (i.e. force strain relationship) of the triceps surae tendon and aponeurosis relate to the performed sport activity in an intensity-dependent manner.

Age-related degeneration in leg-extensor muscle-tendon units decreases recovery performance after a forward fall: compensation with running experience.

The goals of this study were to investigate whether the lower muscle-tendon units (MTUs) capacities in older affect their ability to recover balance with a single-step after a fall, and to examine whether running experience enhances and protects this motor skill in young and old adults. The investigation was conducted on 30 older and 19 younger divided into two subgroups: runners versus non-active. In previous studies we documented that the older had lower leg extensor muscle strength and tendon stiffness while running had no effect on MTUs capacities.

Aging and running experience affects the gearing in the musculoskeletal system of the lower extremities while walking.

The aims of this study were to investigate whether older adults modify their walking mechanics to compensate for the degeneration in their muscle-tendon units (MTUs), and to examine whether running has a beneficial effect on walking mechanics in younger and older adults. The investigation was conducted on 30 older and 19 younger adults divided into two subgroups: runners versus non-active. In previous studies we documented that older adults had lower leg-extensor muscle strength and tendon stiffness compared to younger.

Adaptational phenomena and mechanical responses during running: effect of surface, aging and task experience.

The goals of the study were to identify adaptational phenomena in running mechanics over a variety of surfaces due to age related changes in the muscle-tendon units (MTUs) capacities, to examine whether running experience is associated with adaptational effects on running mechanics over a variety of surfaces even at old age, and to investigate whether surface condition affects running mechanics. The investigation was executed on 30 old and 19 young including 29 runners and 20 non-active subjects. In a previous study we documented that the older had lower MTUs capacities.

Effect of contraction form and contraction velocity on the differences between resultant and measured ankle joint moments.

During a maximal isometric plantar flexion effort the moment measured at the dynamometer differs from the resultant ankle joint moment. The present study investigated the effects of contraction form and contraction velocity during isokinetic plantar/dorsal flexion efforts on the differences between resultant and measured moments due to the misalignment between ankle and dynamometer axes. Eleven male subjects (age: 31+/-6 years, mass: 80.