Deep Active Inference and Scene Construction.

Adaptive agents must act in intrinsically uncertain environments with complex latent structure. Here, we elaborate a model of visual foraging-in a hierarchical context-wherein agents infer a higher-order visual pattern (a "scene") by sequentially sampling ambiguous cues. Inspired by previous models of scene construction-that cast perception and action as consequences of approximate Bayesian inference-we use active inference to simulate decisions of agents categorizing a scene in a hierarchically-structured setting.

Leaf swallowing and parasite expulsion in Khao Yai white-handed gibbons (Hylobates lar), the first report in an Asian ape species.

Leaf swallowing behavior, known as a form of self-medication for the control of nematode and tapeworm infection, occurs widely in all the African great apes (Pan troglodytes schweinfurthii, P. t. troglodytes, P.

Conditional expression of full-length humanized anti-prion protein antibodies in Chinese hamster ovary cells.

Because of their high antigen specificity and metabolic stability, genetically engineered human monoclonal antibodies are on the way to becoming one of the most promising medical diagnostics and therapeutics. In order to establish an in vitro system capable of producing such biosimilar antibodies, we used human constant chain sequences to design the novel human antibody expressing vector cassette pMAB-ABX. A bidirectional tetracycline (tet)-controllable promotor was used for harmonized expression of immunoglobulin type G (IgG) heavy and light chains.

Tetracycline-regulated highly inducible expression of the human prion protein in murine 3T3 cells.

To provide an in vitro system that allows inducible or conditional overexpression of human prion protein (PrP), we have established a tetracycline (Tc)-regulated system in murine 3T3 L1 fibroblast cells. A replacement-type gene targeting vector cassette was constructed to express the human fatal familial insomnia (FFI) prion protein gene (PRNP) under control of a Tc-responsive element. Following stable integration of the vector into 3T3 Tet-Off cells, we have isolated and characterised six 3T3 L1 pTet-Off FFI clones.