48 results match your criteria: "German National Reference Centre for Borrelia[Affiliation]"

Multi-locus sequence typing (MLST) based on eight genes has become the method of choice for Borrelia typing and is extensively used for population studies. Whole-genome sequencing enables studies to scale up to genomic levels but necessitates extended schemes. We have developed a 639-loci core genome MLST (cgMLST) scheme for Borrelia burgdorferi sensu lato (s.

View Article and Find Full Text PDF

Combining short- and long-read sequencing unveils geographically structured diversity in .

iScience

September 2024

Center for Experimental and Molecular Medicine, Amsterdam University Medical Centers, Location Academic Medical Center, Amsterdam, the Netherlands.

is an emerging tick-borne human pathogen in the Northern hemisphere. The aim of the current study was to compare whole genome sequences of isolates from different continents. Using a combination of Illumina and PacBio platforms and a novel genome assembly and plasmid typing pipeline, we reveal that the 21 sequenced isolates and publically available genomes from North America, Asia, and Europe form genetically distinct populations and cluster according to their geographical origin, where distinct species are endemic.

View Article and Find Full Text PDF
Article Synopsis
  • Lyme disease, caused by spirochete bacteria transmitted by ticks, is the most common and rapidly spreading tick-borne illness in Europe and North America.
  • Researchers sequenced the genomes of 47 Lyme disease isolates, revealing a diverse range of species and consistent plasmid features that are crucial for the bacteria's adaptation.
  • The study highlights the genetic complexities involved, such as recombination and rapid evolution, especially in genes that interact with hosts, contributing to the bacteria's virulence while maintaining a mostly clonal population structure.
View Article and Find Full Text PDF

BackgroundLyme borreliosis (LB), caused by (), is the most common tick-borne infection in Germany. Antibodies against are prevalent in the general population but information on temporal changes of prevalence and estimates of seroconversion (seroincidence) and seroreversion are lacking, especially for children and adolescents.AimWe aimed at assessing antibodies against and factors associated with seropositivity in children and adolescents in Germany.

View Article and Find Full Text PDF
Article Synopsis
  • - The Borrelia burgdorferi species complex causes Lyme borreliosis, but understanding the genetic differences among its various genospecies is hindered by challenges in assembling complete genomes due to their complex structures and numerous plasmids.
  • - Previous genome assembly techniques have proven inadequate, but researchers used advanced HiFi PacBio sequencing and a refined workflow to successfully achieve gap-free and high-quality genome assemblies for Borrelia, including both chromosomal and plasmid sequences.
  • - The study concludes that utilizing high-fidelity sequencing and an optimized reconstruction pipeline can effectively resolve complex microbial genomes, potentially benefiting future genomic research on other microorganisms.
View Article and Find Full Text PDF

Beside mosquitoes, ticks are well-known vectors of different human pathogens. In the Northern Hemisphere, Lyme borreliosis (Eurasia, LB) or Lyme disease (North America, LD) is the most commonly occurring vector-borne infectious disease caused by bacteria of the genus Borrelia which are transmitted by hard ticks of the genus Ixodes. The reported incidence of LB in Europe is about 22.

View Article and Find Full Text PDF

Opinion: Methodological Shortcomings in the Study on a Prophage-based PCR Test for Lyme Borreliosis.

Front Microbiol

December 2021

Center for Experimental and Molecular Medicine, Amsterdam Institute for Infection and Immunology, Amsterdam UMC, University of Amsterdam, Amsterdam, Netherlands.

View Article and Find Full Text PDF

Polymyxin resistance, determined by genes located on plasmid DNA, currently poses a high epidemiological threat. Non-typhoid (NTS) are one of the key pathogens causing diarrheal diseases. Here, we report the isolation and whole genome sequencing of multidrug colistin-resistant/susceptible isolates of non-typhoid serovars carrying genes.

View Article and Find Full Text PDF

Lyme borreliosis (LB) caused by spp. is the most common human tick-borne disease in Europe. Although seroprevalence studies are conducted in several countries, rates of seroconversion and seroreversion are lacking, and they are essential to determine the risk of infection.

View Article and Find Full Text PDF

Canine cases of relapsing fever (RF) borreliosis have been described in Israel and the USA, where two RF species, and , can cause similar clinical signs to the in dogs and cats reported from Israel, including fever, lethargy, anorexia, thrombocytopenia, and spirochetemia. In this report, we describe the first clinical cases of two dogs and a cat from Spain (Cordoba, Valencia, and Seville) caused by the RF species Spirochetes were present in the blood smears of all three animals, and clinical signs included lethargy, pale mucosa, anorexia, cachexia, or mild abdominal respiration. Laboratory findings, like thrombocytopenia in both dogs, may have been caused by co-infecting pathogens (i.

View Article and Find Full Text PDF

Members of the Borrelia burgdorferi sensu lato (s.l.) species complex are known to cause human Lyme borreliosis.

View Article and Find Full Text PDF

Background: The genus Borrelia comprises spirochaetal bacteria maintained in natural transmission cycles by tick vectors and vertebrate reservoir hosts. The main groups are represented by a species complex including the causative agents of Lyme borreliosis and relapsing fever group Borrelia. Borrelia miyamotoi belongs to the relapsing fever group of spirochetes and forms distinct populations in North America, Asia, and Europe.

View Article and Find Full Text PDF

Birds are hosts for several zoonotic pathogens. Because of their high mobility, especially of longdistance migrants, birds can disperse these pathogens, affecting their distribution and phylogeography. We focused on Borrelia burgdorferi sensu lato, which includes the causative agents of Lyme borreliosis, as an example for tick-borne pathogens, to address the role of birds as propagation hosts of zoonotic agents at a large geographical scale.

View Article and Find Full Text PDF

species are vector-borne parasitic bacteria with unusual, highly fragmented genomes that include a linear chromosome and linear as well as circular plasmids that differ numerically between and within various species. Strain CA690, which was cultivated from a questing nymph in the San Francisco Bay area, CA, was determined to be genetically distinct from all other described species belonging to the complex. The genome, including plasmids, was assembled using a hybrid assembly of short Illumina reads and long reads obtained via Oxford Nanopore Technology.

View Article and Find Full Text PDF

In 2014, a new tick species, Ixodes inopinatus, was described, which is closely related to Ixodes ricinus. So far, I. inopinatus has been found in Tunisia, Morocco, Spain, Portugal, Romania, Austria, and southern Germany.

View Article and Find Full Text PDF

Population structure of Borrelia turcica from Greece and Turkey.

Infect Genet Evol

January 2020

German National Reference Centre for Borrelia, Bavarian Health and Food Safety Authority, Veterinärstr. 2, Oberschleissheim 85764, Germany.

Borrelia turcica, a member of the reptile-associated Borrelia clade, is vectored by Hyalomma aegyptium. The only suggested reservoir hosts of B. turcica are tortoises of the genus Testudo.

View Article and Find Full Text PDF

First investigations on serum resistance and sensitivity of Borrelia turcica.

Ticks Tick Borne Dis

August 2019

German National Reference Centre for Borrelia, Bavarian Health and Food Safety Authority, Veterinärstr. 2, Oberschleissheim, 85764, Germany.

Borrelia turcica is a reptile-associated Borrelia species that is vectored by the hard tick Hyalomma aegyptium. Tortoises of the genus Testudo represent the principal host of adult H. aegyptium, while immature stages are less host-specific and can be found on various vertebrates and even on humans.

View Article and Find Full Text PDF

DNA purification is a critical step in the processing of samples for molecular diagnosis and/ or identification of pathogens via polymerase chain reaction (PCR). Especially when handling vectors like ticks, purifying the DNA always poses a challenge. In this study, we compared factors that may have an influence on DNA extraction namely commercially available DNA extraction kits vs alkaline hydrolysis for DNA extraction.

View Article and Find Full Text PDF

Core genome phylogenetic analysis of the avian associated Borrelia turdi indicates a close relationship to Borrelia garinii.

Mol Phylogenet Evol

February 2019

National Institute of Health Dr. Ricardo Jorge, Infectious Department, Lisbon, Portugal; MARE - Marine and Environmental Sciences Centre, Department of Life Sciences, Largo Marquês de Pombal, Faculty of Sciences and Technology, University of Coimbra, Portugal.

Borrelia burgdorferi sensu lato comprises a species complex of tick-transmitted bacteria that includes the agents of human Lyme borreliosis. Borrelia turdi is a genospecies of this complex that exists in cryptic transmission cycles mainly between ornithophilic tick vectors and their avian hosts. The species has been originally discovered in avian transmission cycles in Asia but has increasingly been found in Europe.

View Article and Find Full Text PDF
Article Synopsis
  • Borrelia are bacteria transmitted by ticks that cause Lyme disease and relapsing fever; this study examines the newly sequenced genomes of B. turcica and B. tachyglossi, which are linked to reptiles and echidnas, respectively.
  • The genomes of these species are notably similar to those of other Borrelia involved in relapsing fever, containing a large chromosome and multiple plasmids, but also show distinct features like expanded nutrient biosynthesis genes.
  • The findings suggest a unique evolutionary relationship between these Borrelia and Lyme disease-causing Borrelia, highlighting their potential biological significance and the need for further research on their pathogenicity.
View Article and Find Full Text PDF

Background: Lyme borreliosis caused by spirochetes of the Borrelia burgdorferi (sensu lato) complex is still the most common tick-borne disease in Europe, posing a considerable threat to public health. The predominant vector in Europe is the widespread hard tick Ixodes ricinus, which also transmits the relapsing fever spirochete B. miyamotoi as well as pathogenic Rickettsiales (Anaplasma phagocytophilum, Rickettsia spp.

View Article and Find Full Text PDF

Association of Borrelia and Rickettsia spp. and bacterial loads in Ixodes ricinus ticks.

Ticks Tick Borne Dis

January 2018

Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Buenteweg 17, 30559 Hannover, Germany. Electronic address:

In recent years, awareness of coinfections has increased as synergistic or antagonistic effects on interacting bacteria have been observed. To date, several reports on coinfections of ticks with Rickettsia and Borrelia spp. are available.

View Article and Find Full Text PDF