Occurrence and clinical correlates of SARS-CoV-2 viremia in two German patient cohorts.

Viremia defined as detectable SARS-CoV-2 RNA in the blood is a potential marker of disease severity and prognosis in COVID-19 patients. Here, we determined the frequency of viremia in serum of two independent COVID-19 patient cohorts within the German National Pandemic Cohort Network (German: tionales andemie horten etzwerk, NAPKON) with diagnostic RT-PCR against SARS-CoV-2. A cross-sectional cohort with 1,122 COVID-19 patients (German: , SUEP) and 299 patients recruited in a high-resolution platform with patients at high risk to develop severe courses (German: , HAP) were tested for viremia.

A Short Cognitive and Neuropsychiatric Assessment Scale for Progressive Supranuclear Palsy.

Background: Patients with Progressive Supranuclear Palsy (PSP) suffer from several neuropsychological impairments. These mainly affect the frontal lobe and subcortical brain structures. However, a scale for the assessment of cognitive and neuropsychiatric disability in PSP is still missing.

Effectiveness of Root Canal Irrigation and Dressing for the Treatment of Asymptomatic Apical Periodontitis: A Systematic Review Assessing Complementary Timeframes.

Sodium hypochlorite (NaOCl), ethylenediaminetetraacetic acid (EDTA), and calcium hydroxide (Ca(OH)) are used in root canal treatment. However, further research is needed to assess their effectiveness at time points not considered in previous guidelines. This review complements the systematic review by Rossi-Fedele and Rödig (2023) by considering time points excluded by the latter.

Comparing a new visuospatial intervention administered 3 days after a trauma film to reduce the occurrence of intrusive visual memories: a single-center randomized, controlled trial in healthy participants.

Introduction: Intrusive memories occur frequently after potentially traumatic events and form a core symptom of posttraumatic stress disorder (PTSD) if they persist. The translational approach of visuospatial interventions tries to target those intrusive memories in order to reduce their frequency predominantly using an intervention including as one component the computer game Despite promising results, the application of has critical drawbacks, e.g.

Empowerment in structures. Practical-ethical considerations of the preconditions for technology-assisted dementia care in Germany based on an expert-interview study.

Background: Intelligent assistive technologies (IAT) have become more common in dementia care. Ethical reflection on technology-assisted dementia care (TADC) has focused so far mainly on individual and interpersonal implications (e.g.

The anomalous state of Uranus's magnetosphere during the Voyager 2 flyby.

The Voyager 2 flyby of Uranus in 1986 revealed an unusually oblique and off-centred magnetic field. This single in situ measurement has been the basis of our interpretation of Uranus's magnetosphere as the canonical extreme magnetosphere of the solar system; with inexplicably intense electron radiation belts and a severely plasma-depleted magnetosphere. However, the role of external forcing by the solar wind has rarely been considered in explaining these observations.

Revisiting water resources management in the Mandara Mountains.

This article evaluates the prospects for rainwater harvesting (RWH) as a means of optimizing water management in the Mandara Mountains. RWH is a small-scale water conservation approach for locally intercepting and storing rainfall before it enters the usual hydrologic cycle. This ancient practice has recently sustained lives in semiarid areas of the world (e.

Multiomic analyses direct hypotheses for Creutzfeldt-Jakob disease risk genes.

Prions are assemblies of misfolded prion protein that cause several fatal and transmissible neurodegenerative diseases, with the most common phenotype in humans being sporadic Creutzfeldt-Jakob disease (sCJD). Aside from variation of the prion protein itself, molecular risk factors are not well understood. Prion and prion-like mechanisms are thought to underpin common neurodegenerative disorders meaning that the elucidation of mechanisms could have broad relevance.

From Extractables to Exposure Data: Sensitivity Analysis of Extrapolation Algorithms with Focus on USP 〈665〉.

We evaluated algorithms designed to extrapolate extractables data for predicting process equipment-related leachables (PERLs) and assessing PERL exposure in single-use systems (SUSs) and their assemblies. The robustness and sensitivity of these algorithms were tested against variations in input data, including extrapolation algorithms for both short and long contact time extractables data obtained from the standardized extractables protocol provided in USP 〈665〉. Our findings demonstrate that extrapolated data for SUS and assemblies are suitable for safety assessments.

The α-globin super-enhancer acts in an orientation-dependent manner.

Individual enhancers are defined as short genomic regulatory elements, bound by transcription factors, and able to activate cell-specific gene expression at a distance, in an orientation-independent manner. Within mammalian genomes, enhancer-like elements may be found individually or within clusters referred to as locus control regions or super-enhancers (SEs). While these behave similarly to individual enhancers with respect to cell specificity, distribution and distance, their orientation-dependence has not been formally tested.

Assessing learning, behaviour, and stress level in goats while testing a virtual fencing training protocol.

Virtual fencing (VF) is a modern fencing technology using Global Positioning System-enabled collars which emit acoustic signals and, if the animal does not respond, electric pulses. Studies with cattle indicate successful learning and no distinct negative impact on the animals' behaviours and stress level. However, the number of studies testing VF with goats is relatively small.

Supporting rotational grazing systems with virtual fencing: paddock transitions, beef heifer performance, and stress response.

Animal welfare is integral to sustainable livestock production, and pasture access for cattle is known to enhance welfare. Despite positive welfare impacts, high labour requirements hinder the adoption of sustainable grazing practices such as rotational stocking management. Virtual fencing (VF) is an innovative technology for simplified, less laborious grazing management and remote animal monitoring, potentially facilitating the expansion of sustainable livestock production.

Classification of Histologically Proven Inflammation in Clinically Inactive Corneal Scars: Implications for Graft Outcomes After Penetrating Keratoplasty.

: Clinically inactive corneal scars have repeatedly been shown to exhibit histological inflammation. This study aimed to evaluate the degree of histological inflammation in clinically inactive corneal scars of different origins and its correlation with graft rejection and failure following penetrating keratoplasty. : The study included 205 primary corneal explants with clinically inactive central scars resulting from herpes simplex virus keratitis (HSV, = 55), keratoconus ( = 39), mechanical trauma ( = 27), scrophulosa ( = 22) or other/unknown causes ( = 62).

Phenolic and Iridoid Glycosides from L. and Their Effects on the α, δ, and γ Subtypes of the PPAR System-Including the Discovery of the Novel Phenylethanoid Cardiaphenyloside A and the Most Active 7-Chloro-6-desoxy-harpagide.

L. is known in Europe for its cardioactivity-also in interrelation with known risk factors of the metabolic syndrome-just as Houtt. in East Asia; however, up to now, no active constituents could be identified.

Asymmetric Donor-Acceptor 2,7-Disubstituted Fluorenes and Their 9-Diazoderivatives: Synthesis, Optical Spectra and Photolysis.

In a search for dyes photoactivatable with visible light, fluorenes with substituents at positions 2 and 7 were prepared, and their absorption and emission spectra were studied. In particular, the synthesis route to 9-diazofluorenes with 2-(N,N-dialkylamino) and N-modified 7-(4-pyridyl) substituents was established. These compounds are initially non-fluorescent, undergo photolysis with UV or blue light, and-in non-polar media-provide orange- to red-emitting products with a large separation between absorption and emission bands.

Effect of Different Working Settings of Sandblasting on Resin Composite Repair Bond Strength.

To investigate the effect of different sandblasting settings on the shear bond strength (SBS) in the repair of resin composite, specimens (resin composite, enamel, and dentin; each group = 16) were sandblasted by varying the parameters of air pressure (0.2/0.3/0.

Proteomic Profiling Towards a Better Understanding of Genetic Based Muscular Diseases: The Current Picture and a Look to the Future.

Proteomics accelerates diagnosis and research of muscular diseases by enabling the robust analysis of proteins relevant for the manifestation of neuromuscular diseases in the following aspects: (i) evaluation of the effect of genetic variants on the corresponding protein, (ii) prediction of the underlying genetic defect based on the proteomic signature of muscle biopsies, (iii) analysis of pathophysiologies underlying different entities of muscular diseases, key for the definition of new intervention concepts, and (iv) patient stratification according to biochemical fingerprints as well as (v) monitoring the success of therapeutic interventions. This review presents-also through exemplary case studies-the various advantages of mass proteomics in the investigation of genetic muscle diseases, discusses technical limitations, and provides an outlook on possible future application concepts. Hence, proteomics is an excellent large-scale analytical tool for the diagnostic workup of (hereditary) muscle diseases and warrants systematic profiling of underlying pathophysiological processes.

The Intersection of Epigenetics and Senolytics in Mechanisms of Aging and Therapeutic Approaches.

The biological process of aging is influenced by a complex interplay of genetic, environmental, and epigenetic factors. Recent advancements in the fields of epigenetics and senolytics offer promising avenues for understanding and addressing age-related diseases. Epigenetics refers to heritable changes in gene expression without altering the DNA sequence, with mechanisms like DNA methylation, histone modification, and non-coding RNA regulation playing critical roles in aging.

Information Behaviour and Knowledge of Patients Before Radical Prostatectomy.

Background/objectives: Robot-assisted and open radical prostatectomy (RARP and ORP) are established procedures for localized prostate cancer, with comparable oncological and functional outcomes. Little is known about patients' knowledge of both procedures. This study aimed to examine comparatively the informational behaviour and knowledge of patients undergoing ORP vs.

Transcriptional time-course analysis during ash dieback infection revealed different responses in tolerant and susceptible Fraxinus excelsior genotypes.

Hymenoscyphus fraxineus, the causal agent of Ash Dieback (ADB), has been introduced to eastern Europe in the 1990s from where it spread causing decline in European ash populations. However, the genetic basis of the molecular response in tolerant and susceptible ash trees to this disease is still largely unknown. We performed RNA-sequencing to study the transcriptomic response to the disease in four ash genotypes (ADB-tolerant FAR3 and FS36, and ADB-susceptible UW1 and UW2), during a time-course of 7, 14, 21, and 28 days post-inoculation, including mock-inoculated trees as control samples for each sampling time point.

Genomic analysis of three medieval parchments from German monasteries.

In the last two decades there has been growing interest in the analysis of ancient DNA obtained from the parchment used in historic documents. The genetic insight that this data provides makes collections of historic documents an invaluable source for studying the development and spread of historical livestock populations. Additionally, the biological data may provide new information for the historical analysis that could be used to determine the provenance as well as the authenticity of these documents.