Complete plastid genome of (Fisch.) Bunge var. membranaceus.

The complete nucleotide sequence of the (Fisch.) Bunge var. membranaceus chloroplast genome was reported and characterized in this study.

The antioxidative effect of a novel free radical scavenger 4'-hydroxyl-2-substituted phenylnitronyl nitroxide in acute high-altitude hypoxia mice.

Acute mountain sickness is caused by sub-acute hypoxia in healthy individuals going rapidly to altitude. Both tissue hypoxia in vitro and whole-body hypoxia in vivo have been found to promote the release of reactive oxygen species. Nitronyl nitroxide can trap free radicals such as ·NO or ·OH, and may therefore be efficient protective agents.

Pharmacokinetics of aconitine in rat skin after oral and transdermal gel administrations.

The purpose of this study was to evaluate percutaneous penetration and arrhythmogenic effects of aconitine after transdermal administration, compared with the oral route. Skin penetration of aconitine was tested by a microdialysis technique in rats and in vivo recovery was determined by retrodialysis. After oral and transdermal administration of aconitine, dialysate was sampled at 20 min intervals until the end of the experiment for the determination of concentration of aconitine in skin.

[Clinical typing and surgical principle of pressure sore].

Objective: To investigate the clinical typing and their relevant surgical treatment principle and method of pressure sore.

Methods: From January 1983 to April 2006, 122 patients with 179 pressure sores were treated. There were 93 males and 29 females, aging 15-68 years.

[Surgical treatment of traumatic subclavian artery].

Objective: To explore the treatment of traumatic subclavian artery.

Methods: From July 1990 to January 2006, 12 cases of traumatic subclavian artery were treated by using of combined incision of superior-inferior clavian. All patients were male, aging 18-36 years (mean 22.

Liquid chromatography determination of 10-hydroxycamptothecin in human serum by a column-switching system containing a pre-column with restricted access media and its application to a clinical pharmacokinetic study.

A simple, rapid, sensitive column-switching HPLC method is described for the analysis of the 10-hydroxycamptothecin (HCPT) in human serum. A pre-column containing restricted access media (RAM) is used for the sample clean-up and trace enrichment and is combined with a C18 column for the final separation. The analytical time is 8 min.

[The influence of recombinant human growth hormones on the systemic metabolism after severe burn].

Objective: To explore the influence of recombinant human growth hormones (rhGH) postburn systemic metabolism.

Methods: Twenty-four burn patients were randomly and equally divided into treatment and control groups. Same amount of rhGH (9 U/d) or isotonic saline was injected subcutaneously to respective patients during 3 approximately 17 postburn days (PBDs).

Down-regulation of four arsenic antagonists on apoptosis and telomerase activity induced by arsenic trioxide in three myelocytic leukemia cell lines.

Aim: To investigate regulative effects of thiol reagents, N-acetyl-l-cysteine (NAC) and natrii dimercaptosussinas (NDMS), catalase (CAT), and calcium chelator 2-[(2-bis-[carboxymethyl]-amino-5-methyl-phenoxy)-met]-6-methoxy-8-bis-[carboxy-methyl]-aminoquinoline (Quin 2) on apoptosis and telomerase activity induced by arsenic trioxide (As2O3) in three myelocytic leukemia cell lines.

Methods: Flow cytometry was used to examine apoptosis and a PCR ELISA kit was used to detect telomerase activity.

Results: As2O3 induced about 40 % - 60 % of apoptosis in NB4, K562, and HL-60 cells at the concentration of 0.

[Determination of 5-fluorouracil in human serum by high performance liquid chromatography].

A reversed-phase HPLC method is described for determination of 5-fluorouracil in human serum. After being extracted with ethyl acetate 5-fluorouracil was analyzed by reversed-phase HPLC column (Zorbax ODS) and UV-detector (273nm) with tegaful as internal standard. The mobile phase was a mixture of methyl alcohol and water (20:80, V:V) at a flow rate of 1mL/min.

[Properties and applications of restricted access stationary phases].

Several types of restricted access stationary phases used for the analysis of drugs in biological fluids by direct injection into high-performance liquid chromatographic column are introduced in this paper with 20 references These new packings included internal surface reversed phase, semipermeable surface, shielded hydrophobic phase and mixed functional phase. The structural characteristics and the chromatographic properties of these packings are reviewed, especially with regard to their use in the assaying of drugs.

Pharmacokinetics of m-nifedipine in rabbits after intravenous injection.

Aim: To study the dose effects on pharmacokinetics of m-Nif.

Methods: Fifteen rabbits were divided into 3 groups receiving i.v.

[HPLC determination of 4-[4"-(2",2",6",6"-tetramethyl-1"-piperidinyloxy) amino]-4'-demethylepipodophyllotoxin in rat plasma and studies of its pharmacokinetics].

4-[4"-(2",2",6",6"-tetramethyl-1"-piperidinyloxy) amino]-4'- demethylepipodophyllotoxin (GP-7) is a new podophyllotoxin spin-labeled derivative. Its primary effect is the antitumor activity on transplanted mouse tumors and cultured tumor cells. This paper describes a method for its determination using HPLC with UV detection and the determination of its pharmacokinetic parameters in rats.

Effects of 4-[4''-(2'',2'',6'',6''-tetramethyl-1''-piperidinyloxy)amino]-4'-de methylepipodophyllotoxin on immune function in mice.

4-[4''-(2'',2'',6'',6''-Tetramethyl-1''-piperidinyloxy)amino]-4'- demethylepipodophyllotoxin (GP-7) 10-40 mg.kg-1 ip daily for 7 d reduced the specific antibody formation of splenocytes, serum agglutinin titer, and hemolysin HC50 in mice immunized with SRBC. GP-7 inhibited the footpad delayed hypersensitivity reaction and decreased the weights of spleen and thymus, but did not affect the phagocytic function of the peritoneal macrophages.

[Preparation method and quality control of compound Daphne injection].

Compound Daphne Injection (CDI) is a sterilized solution extracted and refined from Cortex Daphnes, Radix Gentianae Macrophyllae, Radix Angelicae Pubescentis and Radix Notopterygii. The preparation method and quality control of CDI are discussed in this paper.