Combined thermosensitive in situ gel with AMD3100 in sutureless technique improves the survival and function of kidney transplants in mice.

The mouse is an optimal animal model for kidney transplantation. Recent reports suggest that application of poloxamer 407, a thermosensitive in situ gel, during the sutureless technique significantly increases animal survival, compared to traditional methods. However, further improvement of this technology is greatly needed but remains unexplored.

MiR-19 suppresses fibroblast-like synoviocytes cytokine release by targeting toll like receptor 2 in rheumatoid arthritis.

Fibroblast-like synoviocytes (FLS) play an important role in the pathogenesis of rheumatoid arthritis (RA) through participating in joint tissue inflammation and joint damage. MicroRNAs are a kind of small non-coding RNAs that can regulate gene expression in the transcription level to affect cell behaviors. This study intended to investigate the expression of miR-19 in FLS from RA patients and related mechanism.

miRNA-101 acts as a tumor suppressor in oral squamous cell carcinoma by targeting CX chemokine receptor 7.

miR-101 is significantly downregulated in various human cancers, including oral squamous cell carcinoma (OSCC). However, the role of miR-101 in OSCC has not been elucidated. In this study, miR-101 lowly expressed in OSCC tissues and cell lines compared with that in adjacent normal tissues and human normal oral keratinocyte cells.

Relationship between PI3K pathway and angiogenesis in CIA rat synovium.

To investigate the expression of hypoxia inducible factor (HIF-1α) and vascular endothelial growth factor (VEGF) in the synovium of collagen-induced arthritis (CIA) joint, and whether the PI3K pathway regulates angiogenesis in rheumatoid arthritis or not. A randomized controlled according to the principle of the rats were divided into normal control group (10 rats) and the experimental group (40 rats). The experimental group rats were established as type II collagen plus adjuvant Freund's complete adjuvant-induced arthritis model.

Lobulated adenomyoepithelioma: a case report showing immunohistochemical profiles.

Lobulated adenomyoepithelioma of the breast is an extremely rare lesion, with hyperplasia of myoepithelial cells and glandular epithelial cells. We present a case of a 51-year-old woman with a small painless hard lump in each breast. The lesion in the left breast was an irregular solid mass, and the right breast showed a subareolar nodule with bloodstained nipple discharge.

Curcumin inhibits the proliferation and invasion of human osteosarcoma cell line MG-63 by regulating miR-138.

Objective: In this study, we screened the different human osteosarcoma cell line MG-63 miRNAs after the treatment of curcumin and explored the effects of curcumin on MG-63 cells and its mechanism.

Methods: Affemitrix miRNA chip was used to detect the changes of miRNA expression profile in MG-63 cells before and after curcumin treatment, and screen different expression of miRNAs. The target gene of miRNA was analyzed by bioinformatics.

Tumor abnormal protein (TAP) examination contributes to primary diagnosis of bladder cancer.

Objective: This study aims to investigate the application value of tumor abnormal protein (TAP) examination in the diagnosis of urothelial carcinoma of the bladder.

Method: Abnormal sugar chain glycoproteins in the peripheral blood of 87 patients with urothelial carcinoma of the bladder were detected, and compared with non-tumor patients accompanied by hematuria.

Result: TAP examination showed that the positive rate of the abnormal sugar chain glycoprotein in the peripheral blood of the 87 patients with urothelial carcinoma of the bladder was 78.

Clinical experience of multiple flaps for the reconstruction of dorsal digital defects.

Objective: In this report, we present our experience with reconstruction of the skin defects on the dorsum of the digits using multiple flaps.

Methods: Between November 2010 and March 2013, 45 patients with dorsal digital defects underwent reconstruction using homodigital dorsal digital fasciocutaneous flap (n = 17), heterodigital dorsal digital fasciocutaneous flap (n = 14), dorsal metacarpal artery fasciocutaneous flap (n = 8) and free venous flaps (n = 6). The average flap size was 1.

Application of IMF screws to assist internal rigid fixation of jaw fractures: our experiences of 168 cases.

Intermaxillary fixation (IMF) screws were first introduced to achieve IMF as a kind of bone borne appliance for jaw fractures in 1989. Because this method can overcome many disadvantages associated with tooth borne appliance, IMF screws have been popularly used for jaw fractures since then. From March 2011 to February 2014, we treated 168 cases with single or multiple jaw fractures by open reduction and a total of 705 IMF screws were intraoperatively applied in all the cases to achieve IMF and maintain dental occlusion as an adjuvant to open reduction.

Curcumin inhibits proliferation of interleukin-22-treated HaCaT cells.

The interleukin (IL)-23/IL-17A/IL-22 cytokine axis plays a critical role in the pathogenesis of psoriasis, wherein IL-22 effects on epidermal alternations by inhibiting differentiation and inducing keratinocyte proliferation. In this study, we investigated effects of curcumin on IL-22-induced proliferation in a human keratinocyte cell line (HaCaT) in vitro experiment. The HaCaT cells growth was measured by a Cell Counting Kit-8.

Upregulation of miR-195 enhances the radiosensitivity of breast cancer cells through the inhibition of BCL-2.

Aims: To investigate the effect of miR-195 overexpression on radiosensitivity and the relevant molecular mechanisms in breast cancer.

Methods: miR-195 mimics were transfected to breast cancer cell line MCF-7 by gene transfection; miR-195 expression level in cells was determined by real-time quantitative PCR. Cellular viability was observed by colony formation assay after the cells were irradiated with X-ray.

JAK2/STAT3 pathway mediating inflammatory responses in heatstroke-induced rats.

Heatstroke not only directly induces cell injury, but also causes large amounts of inflammatory mediators release and cells with extensive biological activities to induce a systemic inflammatory response and immune dysfunction. This study aimed to observe the effects of JAK2 inhibitor AG490 on the brain injury and inflammatory responses of rats with systemic heatstroke. Under the light microscope, the hippocampus tissues of rat with heatstroke were edema and apoptotic rate was increased.

Study on the consistency of doctor-report outcomes and patient-report outcomes in symptoms of traditional Chinese Medicine: a small sample size trial in diabetics.

Patient-Reported Outcomes (PROs) data are increasingly common and widely accepted in clinical investigations of symptoms. Agreements or differences regarding Doctor-Report Outcomes (DROs) VS PROs data are unclear. In this study, we conducted a clinical trial and investigated the agreement levels of DROs VS PROs in symptoms of diabetics.

Association of OPN overexpression with tumor stage, differentiation, metastasis and tumor progression in human laryngeal squamous cell carcinoma.

Background: Osteopontin (OPN) is overexpressed in many human tumors and involved in promotion of cancer cells by regulating various facets of tumor progression such as cell proliferation, invasion and metastasis. To understand roles of OPN in tumor progression of laryngeal squamous cell carcinoma (LSCC) or develop molecular marker for prognosis and treatment of LSCC, we thus explore biological function of OPN and correlation with p53 in LSCC.

Methods: The expression of OPN and p53 in tumor tissues of LSCC was determined immunohistochemically in both LSCC and adjacent normal tissues.

Hydrogen sulfide accelerates wound healing in diabetic rats.

Aim: The aim of this study was to explore the role of hydrogen sulfide on wound healing in diabetic rats.

Methods: Experimental diabetes in rats was induced by intraperitoneal injection of streptozotocin (STZ) (in 0.1 mol/L citrate buffer, Ph 4.

Effect of pollen typhae on inhibiting autophagy in spinal cord injury of rats and its mechanisms.

Purpose: To investigate the effects and mechanism of pollen typhae on spinal cord injury (SCI) in rats.

Methods: The SCI model was built and animals were randomly divided into three groups according to different concentrations of pollen typhae. Protein, mRNA, and fluorescence expression levels of light-chain-3 (LC-3) and Beclin-1 were determined by western blotting (WB), real-time PCR and immunofluorescence, along as Akt and mammalian target of rapamycin (mROT) by WB.

Hyperbaric oxygen treatment reduced the lung injury of type II decompression sickness.

Objective: To detect the ultrastructural changes in rabbits with type II decompression sickness (DCS), and study the therapeutic effects of hyperbaric oxygen (HBO).

Methods: Twenty-seven male New Zealand rabbits were randomly divided equally into the DCS group, HBO treatment group and control group. Experimental models of each group were prepared.

Maxillary aggressive angiomyxoma showing ineffective to radiotherapy: a rare case report and review of literature.

Aggressive angiomyxoma, mostly originating in the female pelvis and peritoneum or in the male analogous sites, is a rare mesenchymal neoplasm characterized with infiltrative growth to adjacent tissue and local recurrence after primary excision. Herein, we report a case of aggressive angiomyxoma of maxilla in a 60-year-old male patient for its rarity. The patient presented with a one-year history of progressively enlarging maxillary mass on left side.

Clinicopathological features of type AB thymoma with liver metastases.

Objective: We studied the clinicopathological features of type AB thymoma with liver metastases and explore the histological types, diagnosis and differential diagnosis, treatment and prognosis for this disease.

Methods: Liver metastasis specimens were derived from one case of type AB thymoma 5 years after operation and were examined histologically using light microscopy and immunohistochemistry. A comprehensive analysis was performed on the patient's clinical manifestations, histopathology, immunohistochemistry features, differential diagnosis, treatment and prognosis in combination with a review of the relevant literature.

Gallstone ectopia in the lungs: case report and literature review.

Gallstone ectopia in the lungs is relatively rare, which accounts for its frequent misdiagnosis. This paper reports a case found in a suspicious lung cancer surgery. The patient received intrahepatic duct stone removal surgery and partial hepatectomy five months prior to the report.

MicroRNA-543 acts as an oncogene by targeting PAQR3 in hepatocellular carcinoma.

MicroRNAs (miRNAs) are small, non-coding RNAs that can act as oncogenes or tumor suppressor genes in human cancer. Increasing evidences indicate that deregulation of miRNAs contributes to the hepatocarcinogenesis. In this study, we demonstrated that the levels of miR-543 were dramatically increased in clinical hepatocellular carcinoma (HCC) tissues and cell lines.

Vicious cycle of TGF-β signaling in tumor progression and metastasis.

TGF-β is an important biological mediator. It regulates a wide range of functions including embryonic development, wound healing, organ development, immuno-modulation, and cancer progression. Interestingly, TGF-β is known to inhibit cell growth in benign cells but promote progression in cancer cells, a phenomenon known as TGF-β paradox.

Parathyroid hormone induces epithelial-to-mesenchymal transition via the Wnt/β-catenin signaling pathway in human renal proximal tubular cells.

Epithelial-to-mesenchymal transition (EMT) has been shown to play an important role in renal fibrogenesis. Recent studies suggested parathyroid hormone (PTH) could accelerate EMT and subsequent organ fibrosis. However, the precise molecular mechanisms underlying PTH-induced EMT remain unknown.

Expression of Wnt-5a and β-catenin in primary hepatocellular carcinoma.

It has been reported that changes in Wnt5a expression are closely related to hepatocellular carcinoma (HCC) development, while decreased or abnormal β-catenin expression may promote the invasion and metastasis of tumor cells. In this study, the roles and clinical significance of Wnt-5a and β-catenin expression were analyzed in primary HCC. Real-time PCR (RT-PCR) analysis of Wnt-5a mRNA expression was performed in 26 fresh HCC samples and the corresponding para-carcinoma tissues.

Ultrasonographic features of adenomyoepithelial adenosis: a case report and literature review.

Adenomyoepithelial adenosis of the breast is extremely rare. We treated a 35-year-old woman with two small painless hard lumps in her breasts. On ultrasonography, the lesion in the right breast (upper inner quadrant) showed heterogeneous echogenicity and irregular, well-defined, uniformly hypoechoic nodules separated by funiform hyperechoic areas.