Molecular and Genomic Investigation Unveils Pseudomonas putida as an Emerging Multidrug-Resistant Pathogen Linked to Bovine Clinical Mastitis.

Pseudomonas putida is one of the emerging pathogens responsible causing mastitis in lactating animals. This study investigated the prevalence, antimicrobial resistance (AMR), genetic diversity and virulence factor genes (VFGs) to highlight the pathogenic potentials of P. putida strains isolated from milk, feces and farm soil of bovine clinical mastitis (CM).

Enhancing physio biochemical traits and yield of common buckwheat Fagopyrum esculentum with rice husk biochar and nano iron oxide under water stress.

Climate change is making droughts more frequent, which is a major problem for crop yield, especially for crops that are vulnerable to drought, such as common buckwheat (Fagopyrum esculentum). Drought stress affects negatively on physiological and biochemical processes of plants, leading to reduced yields. This study addresses the knowledge gap regarding effective strategies to mitigate drought-induced damage and enhance productivity in buckwheat.

High throughput screening identifies potential inhibitors targeting trimethoprim resistant DfrA1 protein in Klebsiella pneumoniae and Escherichia coli.

The DfrA1 protein provides trimethoprim resistance in bacteria, especially Klebsiella pneumoniae and Escherichia coli, by modifying dihydrofolate reductase, which reduces the binding efficacy of the antibiotic. This study identified inhibitors of the trimethoprim-resistant DfrA1 protein through high-throughput computational screening and optimization of 3,601 newly synthesized chemical compounds from the ChemDiv database, aiming to discover potential drug candidates targeting DfrA1 in K. pneumoniae and E.

Molecular screening and dynamics simulation reveal potential phytocompounds in Swertia chirayita targeting the UspA1 protein of Moraxella catarrhalis for COPD therapy.

Chronic obstructive pulmonary disease (COPD) is a global health burden, with Moraxella catarrhalis significantly contributing to acute exacerbations and increased healthcare challenges. This study aimed to identify potential drug candidates in Swertia chirayita, a traditional Himalayan medicinal plant, demonstrating efficacy against the ubiquitous surface protein A1 (UspA1) of M. catarrhalis through an in-silico computational approach.