Ginsenoside Rg1 Alleviates Blood-Milk Barrier Disruption in Subclinical Bovine Mastitis by Regulating Oxidative Stress-Induced Excessive Autophagy.

As a critical disease usually infected by , with a worldwide effect on dairy animals, subclinical mastitis is characterized by persistence and treatment resistance. During mastitis, the blood-milk barrier (BMB)'s integrity is impaired, resulting in pathogen invasion and milk quality decline. In this study, it was found that ginsenoside Rg1 (Rg1), a natural anti-inflammatory and antioxidant compound derived from ginseng, inhibited the onset of tight junction (TJ) dysfunction and ameliorated lipoteichoic acid (LTA)-induced BMB disruption inside and outside the organisms.

Effects of Oregano Essential Oil and/or Yeast Cultures on the Rumen Microbiota of Crossbred Simmental Calves.

This study hypothesized that combining oregano essential oil () and yeast cultures (s) would modulate rumen microbiota to promote gastrointestinal homeostasis and function. Twenty-four newborn, healthy, disease-free, crossbred Simmental male calves (birth weight ≥ 35 kg) were assigned to one of four treatments based on birth data. Treatments were as follows: (1) Control (), calves fed calf starter without additives; (2) , calves fed calf starter containing 60 mg/kg body weight () of OEO per day; (3) s, calves fed calf starter containing 45 mg/kg BW of YC per day; and (4) , calves fed calf starter with OEO (60 mg/kg, BW) and YC (45 mg/kg, BW) combination.

Integrated removal of chromium, lead, and cadmium using nano-zero-valent iron-supported biochar: Mechanistic insights and eco-toxicity assessment.

The contamination of water and soil by heavy metals (HMs) is a global issue that should be given much more concern. Modified nano-zero-valent iron (nZVI) composites offer an effective strategy for HMs remediation, but few studies have focused on removing coexisting HMs and the eco-toxicity of the composite. In this study, corn straw biochar-supported nZVI composites (nZVI-BC) were synthesized, characterized and used for the removal of Cr, Pb, and Cd in single and multi-system at different composites dosages, metal concentrations, and solution pH.

Beclin1 regulates yak endometrial inflammation and TLR4/NF-κB signaling pathway through autophagy/non-autophagy function.

Beclin1 is an autophagy related factor, and it is capable of mediating non-autophagy functions, too. Yak endometritis represents a significant obstetric ailment that impedes the normal breeding process. The current understanding of the beclin1 effect on endometrial inflammation in yak remains limited.

De novo assembly of the mitochondrial genome of Glycyrrhiza glabra and identification of two types of homologous recombination configurations caused by repeat sequences.

Background: Glycyrrhiza glabra, which is widely used in medicine and therapy, is known as the 'king of traditional Chinese medicine'. In this study, we successfully assembled and annotated the mitochondrial and chloroplast genomes of G. glabra via high-throughput sequencing technology, combining the advantages of short-read (Illumina) and long-read (Oxford Nanopore) sequencing.

Screened of long non-coding RNA related to wool development and fineness in Gansu alpine fine-wool sheep.

Wool growth and fineness regulation is influenced by some factors such as genetics and environment. At the same time, lncRNA participates in numerous biological processes in animal production. In this research, we conducted a thorough analysis and characterization of the microstructure of wool, along with long non-coding RNAs (lncRNAs), their target genes, associated pathways, and Gene Ontology terms pertinent to the wool fineness development.

The P-type ATPase gene AHA5 is involved in proanthocyanidins accumulation in Medicago truncatula.

Proanthocyanidins (PAs) are the second most abundant plant phenolic natural products. The proton membrane H-ATPase (AHA) is required for PA transportation in vacuoles, but it remains unclear which AHA gene(s) encode tonoplast proton pump in M. truncatula.

The significant role of vegetation activity in regulating wetland methane emission in China.

Accurate quantifying of methane (CH) emissions is a critical aspect of current research on regional carbon budgets. However, due to limitations in observational data, research methodologies, and an incomplete understanding of process mechanisms, significant uncertainties persist in the assessment of wetland CH fluxes in China. In this study, we developed a machine learning model by integrating measured CH fluxes with related environmental data to produce a high-resolution (1 km) dataset of CH fluxes from China's wetlands for the period 2000-2020.

Detection and analysis of enzootic nasal tumor virus 2 in China.

Enzootic nasal tumor virus 2 (ENTV2), the etiologic agent of enzootic nasal adenocarcinoma (ENA) in goats, is highly prevalent in China and causes significant economic losses to the goat industry. Here we describe the occurrence of ENA on a Dazu black goat farm in Chongqing City. At autopsy, nasal cavity masses were observed within the nose of an affected goat; histologically, the tumor was a nasal adenocarcinoma.

Isolation and identification of the causal agent of gummy stem blight disease in Cucumis sativus caused by a bacterial pathogen in China.

Recently, a new bacterial disease was detected on cucumber stalks. In order to study the pathogenesis of this disease, the pathogenic bacteria were isolated and identified on the basis of morphological and molecular characteristics, and further analyzed for pathogenicity and antagonistic evaluation. Pathogenicity analysis showed that HlJ-3 caused melting decay and cracking in cucumber stems, and the strain reisolated from re-infected cucumber stalks was morphologically identical to HlJ-3 colonies, which is consistent with the Koch's postulates.

Analysis of full length transcriptome and resistance characteristics of Atraphaxis bracteata under drought.

Drought is one of the main environmental factors affecting plant survival and growth. Atraphaxis bracteata is a common desert plant mainly utilized in afforestation and desertification control. This study analyzed the morphological, physiological and molecular regulatory characteristics of different organs of A.

Arbuscular mycorrhizal fungi mitigate cadmium stress in maize.

Soil cadmium (Cd) pollution poses a significant environmental threat, impacting global food security and human health. Recent studies have highlighted the potential of arbuscular mycorrhizal (AM) fungi to protect crops from various heavy metal stresses, including Cd toxicity. To elucidate the tolerance mechanisms of maize in response to Cd toxicity under AM symbiosis, this study used two maize genotypes with contrasting Cd tolerance: Zhengdan958 (Cd-tolerant) and Zhongke11 (Cd-sensitive).

A lightweight weed detection model for cotton fields based on an improved YOLOv8n.

In modern agriculture, the proliferation of weeds in cotton fields poses a significant threat to the healthy growth and yield of crops. Therefore, efficient detection and control of cotton field weeds are of paramount importance. In recent years, deep learning models have shown great potential in the detection of cotton field weeds, achieving high-precision weed recognition.

Genomic microsatellite characterization and development of polymorphic microsatellites in Eospalax baileyi.

Microsatellite markers are cost-effective, rapid, efficient, and show great advantages in in large-sample kinship analysis and population structure studies. However, microsatellite loci are seriously underdeveloped in non-model organisms. The plateau zokor (Eospalax baileyi) is a key species living underground in the Tibetan Plateau, the effective management of which has long been challenging.

From laboratory to field: cross-domain few-shot learning for crop disease identification in the field.

Few-shot learning (FSL) methods have made remarkable progress in the field of plant disease recognition, especially in scenarios with limited available samples. However, current FSL approaches are usually limited to a restrictive setting where base classes and novel classes come from the same domain such as PlantVillage. Consequently, when the model is generalized to new domains (field disease datasets), its performance drops sharply.

Phosphate addition intensifies the increase in NO emission under nitrogen deposition in wet meadows of the Qinghai-Tibet Plateau.

Alpine wet meadows are known as NO sinks due to nitrogen (N) limitation. However, phosphate addition and N deposition can modulate this limitation, and little is known about their combinative effects on NO emission from the Qinghai-Tibet Plateau in wet meadows. This study used natural wet meadow as the control treatment (CK) and conducted experiments with N (CONH addition, N15), P (NaHPO addition, P15), and their combinations (CONH and NaHPO addition, N15P15) to investigate how N and P supplementation affected soil NO emissions in wet meadow of QTP.

Grazing increases the complexity of networks and ecological stochastic processes of mycorrhizal fungi.

Arbuscular mycorrhizal fungi (AMF) form extensive symbiotic relationships with plants, which are critical for plant-driven biogeochemical cycles and ecosystem functions. Grazing and mowing, which are common grassland utilization patterns globally, significantly alter plant community characteristics as well as soil nutrients and structure, thereby potentially influencing AMF communities. However, the effects of these grassland managements on AMF community structure and ecological processes remain unclear.

New insights into the tenderization pattern of yak meat by ROS-ERS: Promotion of ERS-associated apoptosis through feedback regulation of PERK/IRE1/ATF6 and caspase-12 activity.

This study aimed to investigate the molecular mechanisms of reactive oxygen species (ROS)-induced endoplasmic reticulum stress (ERS) in apoptosis and meat tenderization during postmortem aging. Yak longissimus dorsi muscle was incubated with N-acetylcysteine (NAC), 4-phenylbutyric acid (4-PBA) and NAC + 4-PBA, respectively, and stored at 4 °C for 0 h, 12 h, 24 h, 72 h, 120 h and 168 h. The results showed that NAC and 4-PBA treatments significantly reduced ROS content and endoplasmic reticulum stress levels.

Effects of nitrogen deposition on soil nitrogen fractions and enzyme activities in wet meadow of the Qinghai-Tibet Plateau.

Soil nitrogen (N) transformation is an essential portion of the N cycle in wetland ecosystems, governing the retention status of soil N by controlling the effective soil N content. N deposition produced by human activities changes the physical characteristics of soil, affecting N fractions and enzyme activities. To characterize these influences, three different N addition levels (N5, 5 g/m; N10, 10 g/m; N15, 15 g/m) were established using a wet meadow on the Qinghai-Tibet Plateau (QTP) as a control treatment (0 g/m).

Genome-wide identification of high-affinity nitrate transporter 2 (NRT2) gene family under phytohormones and abiotic stresses in alfalfa (Medicago sativa).

The high-affinity nitrate transporter 2 (NRT2) protein plays an important role in nitrate uptake and transport in plants. In this study, the NRT2s gene family were systematically analyzed in alfalfa. We identified three MsNRT2 genes from the genomic database.

Response of soil organic carbon and soil aggregate stability to changes in land use patterns on the Loess Plateau.

Land use change can significantly alter the proportion of soil aggregates, thereby influencing aggregate stability and distribution of soil organic carbon (SOC). However, there is minimal research on the variations in the distribution of soil aggregates, aggregate stability, and SOC in soil aggregates following land use change from farmland (FL) to forest and grassland in the Loess Plateau region of China. Select six land use patterns (farmland (FL), abandoned cropland (ACL), Medicago sativa (MS), natural grassland (NG), Picea asperata Mast.

CircRNA profiling reveals the regulatory role of circPAN3 in Hezuo boars Sertoli cell growth.

Background: The Hezuo (HZ) pig, a famous indigenous breed in China, is characterized by precocious puberty compared with foreign-introduced pig breeds. Sexual maturation is a complex physiological process, and in recent years, circular RNAs (circRNAs), a new class of noncoding RNAs with endogenous regulatory functions, have been shown to play important roles in regulating sexual maturation. However, the dynamic expression and regulatory mechanism of circRNAs during sexual maturation in HZ pigs remain unclear.

Optimization of the quality of sea buckthorn juice by enzymatic digestion and inoculation sequence.

Sea buckthorn, rich in nutrients and bioactive compounds such as phenolics, fatty acids, and vitamins, presents processing challenges due to its intense sourness and bland flavor. This study addresses key challenges in flavor enhancement and sourness reduction by evaluating the effects of pectinase treatment and inoculation sequences on the overall quality. Optimal malic acid degradation and antioxidant occurred when Schizosaccharomyces pombe (S.