Industrialization drives the gut microbiome and resistome of the Chinese populations.

Unlabelled: Industrialization has driven lifestyle changes in eastern and western Chinese populations, yet we have a poor understanding of the dynamic changes in their gut microbiome and resistome under industrialization, which is essential for the scientific management of public health. Here, this study employed metagenomics to analyze the gut microbiota of 1,382 healthy individuals from China, including 415 individuals from the eastern region of advanced industrialization and 967 individuals from the western region of developing industrialization. Compared with western populations, eastern populations show a significant increase in interindividual dissimilarity of microbial species composition and metabolic pathways but a significant decrease in intraindividual species and functional diversity.

Response of plant life forms and soil physical properties to near-natural restoration measures in alpine grassland, Tibetan plateau.

Introduction: Near-natural restoration measures enhance the stability of plant life forms in degraded grasslands, facilitating the natural succession of plant communities.

Methods: In this study, we investigated the effects of three natural restoration measures on the alpine meadows of the northeastern Tibetan Plateau: banned grazing (BG), rest grazing (RG), traditional grazing (TG), and continuous grazing (CG). We utilized redundancy analysis (RDA), variation partitioning(VP), hierarchical partitioning (HP), and partial least squares pathway modeling (PLS-PM) to dissect the quantitative relationships between the distribution of plant life forms and soil physical properties under these restoration measures.

Identification of the grape MAPKKK gene family and functional analysis of the VaMAPKKK15 gene under low temperature stress.

Mitogen-activated protein kinase kinase kinases (MAPKKK, MAP3K, MKKK or MEKK) regulate plant growth and development and are involved in regulatory pathways for plant resistance to a wide range of biotic and abiotic stresses. However, the phylogeny of the MAPKKK gene family and the biological function of VaMAPKKK15 under low-temperature stress in Vitis vinifera (V. vinifera) and Vitis amurensis (V.

Biochar Addition Reduces the Effect of High Nitrogen on Soil-Microbial Stoichiometric Imbalance in Abandoned Grassland on the Loess Plateau of China.

Progressively higher atmospheric nitrogen (N) deposition increasingly affects soil ecosystems' elemental cycling and stability. Biochar (BC) amendment has emerged as a possible means of preserving soil system stability. Nevertheless, the pattern of soil-microbial nutrient cycling and system stability in response to BC after high N deposition in ecologically sensitive regions remains uncertain.

Studies on fatty acids and microbiota characterization of the gastrointestinal tract of Tianzhu white yaks.

Introduction: The gut microbiota significantly influences the host's production performance and health status, with different gastrointestinal tissues exhibiting functional diversity reflected in their microbial diversity.

Methods: In this study, five adult male Tianzhu white yaks (4.5 years old) were selected and fed under the same nutritional conditions.

A review of the interaction mechanisms between jasmonic acid (JA) and various plant hormones, as well as the core regulatory role of MYC2.

Jasmonic acid (JA), as a defensive plant hormone, can synergistically or antagonistically interact with common hormones such as gibberellin (GA), abscisic acid (ABA), indole-3-acetic hormone acid (IAA), and ethylene (ETH) during the plant growth process, as well as interact with hormones such as melatonin (MT), brassinolide (BR), and resveratrol to regulate plant growth and development processes such as metabolite synthesis, pest and disease defense, and organ growth. The core regulatory factor MYC2 of JA mainly mediates the signal transduction pathways of these hormone interactions by interacting with other genes or regulating transcription. This article reviews the mechanism of cross-talk between JA and hormones such as ABA, GA, and salicylic acid (SA), and discusses the role of MYC2 in hormone interactions.

Collagen peptides alleviate hyperglycemia in mice by modulating insulin resistance, glucose metabolism and gut microbiota.

In vitro studies have demonstrated that collagen peptides (CP) inhibit the activity of dipeptidyl peptidase-IV. However, the relationship between the AMPK-mediated insulin signaling pathway and gut microbiota modulation in Type 2 Diabetes Mellitus (T2DM) remains underexplored. Current research investigated the hypoglycemic mechanisms associated with CP intervention in a T2DM mouse model induced by a high-fat diet and streptozotocin (STZ).

Allelopathic and auto-toxic effects of aqueous extracts of Codonopsis pilosula root secretions.

Water-soluble constituents in the root exudates of C. pilosula exhibit both allelopathic and auto-toxic properties, which substantially impede its growth. To mitigate the constraints associated with the continuous cropping of C.

Effects of combined application of nitrogen, phosphorus, and potassium fertilizers on seed yield, seed quality and economic returns of Elymus nutans in alpine region.

The Qinghai-Tibetan Plateau (QTP), one of the most important ecological regions in the world, is experiencing a decline in ecological function as a result of severe grassland degradation. Elymus nutans is one of the ecological grass species for restoring degraded grasslands in QTP. The seed yield and seed quality are often limited by soil nutrients in QTP, so it is very important to optimize the application rates of fertilizer for E.

Coevolutionary dynamics in the grass-livestock social-ecological system of China's alpine pastoral areas: A case study of the Qilian Mountains region in China.

Evaluating the dynamic co-evolution and feedback mechanisms within socio-ecological systems is crucial for determining the resilience and sustainability of environmental governance strategies. The grass-livestock system, as a complex entity encompassing livestock nutrition, foraging behavior, vegetation ecology, pastoralists' economic income, and policy interventions, indicates that any change in a single element may trigger a chain reaction within the system. This paper uses a system dynamics approach to construct a simulation model of the grass-livestock system in alpine pastoral areas, simulating the long-term dynamic co-evolution of the socio-ecological system in the Qilian Mountains region of China.

Research on mixed sample test for the rapid detection of rice stripe virus and southern rice black-streaked dwarf virus in vector.

Rice is a staple food in East and Southeast Asian countries, rice disease surveillance and warning is particularly important. Rice stripe disease and southern rice black-streaked dwarf virus disease are the insect-borne viral diseases that easily break out and quickly become epidemic. It is important to improve the efficiency of the virus detection method.

LEHP-DETR: A model with backbone improved and hybrid encoding innovated for flax capsule detection.

Flax, as a functional crop with rich essential fatty acids and nutrients, is important in nutrition and industrial applications. However, the current process of flax seed detection relies mainly on manual operation, which is not only inefficient but also prone to error. The development of computer vision and deep learning techniques offers a new way to solve this problem.

Joint exploration of network pharmacology and metabolomics on the effects of traditional Chinese medicine compounds in weaned yaks.

Introduction: Chinese herbal medicines are relatively inexpensive and have fewer side effects, making them an effective option for improving health and treating diseases. As a result, they have gained more attention in recent years. The weaning period is a critical stage in the life of yaks, often inducing stress in calves.

MicroRNA-203-3p participates in antiviral immune response by negatively regulating TRAF3 in the rainbow trout (Oncorhynchus mykiss).

MicroRNAs (miRNAs) are highly conserved endogenous non-coding RNAs that play a crucial role in fish immune response by regulating gene expression at the post-transcriptional level. In recent years, the viral diseases caused by infectious hematopoietic necrosis virus (IHNV) have caused significant economic losses in rainbow trout (Oncorhynchus mykiss) aquaculture, whereas the immune regulatory mechanisms of miRNAs involved in rainbow trout resistance to IHNV infection remains largely undefined. In this study, we analyzed the structural characteristics of Oncorhynchus mykiss tumor necrosis factor receptor-associated factor 3 (OmTRAF3) by bioinformatics software and explored the molecular mechanism of miR-203-3p in rainbow trout resistance to IHNV by regulating OmTRAF3 in vivo and in vitro.

Characterization of a cell-adapted completely attenuated genotype GIIa porcine epidemic diarrhea virus strain.

Porcine epidemic diarrhea virus (PEDV) has caused significant harm to the global pig industry since its discovery. In this study, a highly pathogenic strain of GIIa PEDV CH/HBXT/2018, isolated previously, was continuously passaged in Vero cells up to passage (P)240, resulting in a completely attenuated virus. The proliferation characteristics of different passages of the strain in Vero cells, pathogenicity in newborn piglets, and mutations in S gene sequence indicated that as the passage number increased, the replication efficiency of PEDV in Vero cells gradually improved, with a more pronounced cytopathic effect.

Life History Strategies of the Winter Annual Plant (Asteraceae) in a Cold Desert Population.

Turcz. is a winter annual species of the Asteraceae family, distributed in sandy areas of northern China, and is crucial for wind avoidance and sand fixation. To understand the inter- and intra-annual population dynamics of in its cold desert habitats, we conducted long- and short-term demographic studies to investigate the timing of germination, seedling survival, soil seed bank and seed longevity of natural populations on the fringe of the Tengger Desert.

Nano-Zinc Oxide Can Enhance the Tolerance of Apple Rootstock M9-T337 Seedlings to Saline Alkali Stress by Initiating a Variety of Physiological and Biochemical Pathways.

Soil salinization severely restricts the growth and development of crops globally, especially in the northwest Loess Plateau, where apples constitute a pillar industry. Nanomaterials, leveraging their unique properties, can facilitate the transport of nutrients to crops, thereby enhancing plant growth and development under stress conditions. To investigate the effects of nano zinc oxide (ZnO NP) on the growth and physiological characteristics of apple self-rooted rootstock M9-T337 seedlings under saline alkali stress, one-year-old M9-T337 seedlings were used as experimental materials and ZnO NPs were used as donors for pot experiment.

Integrated Effects of Water and Nitrogen Coupling on Eggplant Productivity, Fruit Quality, and Resource Use Efficiency in a Cold and Arid Environment.

In order to explore the water and fertilizer requirements of eggplants in the western oasis of the river, the experiment was conducted in Minle County of Gansu Province in 2022 and 2023 under three water stress gradients and three nitrogen application levels: (1) moderate water stress (W, 50-60% in field water capacity [FC]), mild water stress (W, 60-70% in FC), and full irrigation (W, 70-80% in FC); (2) low nitrogen (N, 215 kg·ha), medium nitrogen (N, 270 kg·ha), and high nitrogen (N, 325 kg·ha). Moderate and mild water stress were applied during eggplant flowering and fruiting while full irrigation was provided during the other growth stages; a control class (CK) was established with full irrigation throughout the whole plant growth without nitrogen application. This study investigated the effects of water-saving and nitrogen reduction on the yield, quality, and water-nitrogen use efficiency of eggplants in a cold and arid environment in the Hexi Oasis irrigation area of China.

Luteolin Alleviates Ulcerative Colitis in Mice by Modulating Gut Microbiota and Plasma Metabolism.

Background/objectives: Ulcerative colitis (UC) is a chronic and easily recurrent inflammatory bowel disease. The gut microbiota and plasma metabolites play pivotal roles in the development and progression of UC. Therefore, therapeutic strategies targeting the intestinal flora or plasma metabolites offer promising avenues for the treatment of UC.

Developmental Proteomics Reveals the Dynamic Expression Profile of Global Proteins of (Parthenogenesis).

is used as an experimental animal model for the study of three-host ticks due to its special life cycle and easy maintenance in the laboratory and in its reproduction. The life cycle of goes through a tightly regulated life cycle to adapt to the changing host and environment, and these stages of transition are also accompanied by proteome changes in the body. Here, we used the isobaric tags for a relative and absolute quantification (iTRAQ) technique to systematically describe and analyze the dynamic expression of the protein and the molecular basis of the proteome of in seven differential developmental stages (eggs, unfed larvae, engorged larvae, unfed nymphs, engorged nymphs unfed adults, and engorged adults).

Identification of Gene Family and Expression Analysis of Salt Tolerance in .

is one of the typical ecological grass species, characterized by its strong salt tolerance. Hexokinase (HXK) plays a crucial role in plant growth, development, and resistance to abiotic stresses. To understand the function of in the salt tolerance of , this study identified and analyzed the gene family members using the whole-genome data of .

Proteomics Reveals the Response Mechanism of Embryonic Bovine Lung Cells to Infection.

() has caused huge economic losses to the cattle industry. The interaction between and host cells is elucidated by screening and identifying the target protein of adhesin on the surface of the host cell membrane. However, the response mechanism of embryonic bovine lung (EBL) cells to infection is not yet fully understood.

Molecular Docking Studies and In Vitro Activity of Pancreatic Lipase Inhibitors from Yak Milk Cheese.

Pancreatic lipase serves as a primary trigger for hyperlipidemia and is also a crucial target in the inhibition of hypercholesterolemia. By synthesizing anti-hypercholesterolemic drugs such as atorvastatin, which are used to treat hypercholesterolemia, there were some side effects associated with the long-term use of statins. Based on this idea, in the present study, we identified peptides that inhibited PL by virtual screening and in vitro activity assays.

Exogenous 24-Epibrassinolide Improves Low-Temperature Tolerance of Maize Seedlings by Influencing Sugar Signaling and Metabolism.

Low-temperature (LT) stress seriously affects the distribution, seedling survival, and grain yield of maize. At the seedling emergence stage, maize's coleoptile is one of the most sensitive organs in sensing LT signaling and, in general, it can envelop young leaves to protect them from LT damage. In addition, brassinolides (BRs) have been shown to enhance LT tolerance from various species, but the effects of BRs on coleoptiles in maize seedlings under LT stress are unclear.