Water and nitrogen regulation strategy for wolfberry farmland based on nitrogen balance in the Yellow River irrigation districts of Gansu Province, China.

Agricultural production frequently encounters challenges, including soil nitrogen pollution and imbalances resulting from improper irrigation and fertilization practices. This study focuses on wolfberry farmland, analyzing the effects of four irrigation levels [full irrigation (W0, 75%-85% θ), mild water deficit (W1, 65%-75% θ), moderate water deficit (W2, 55%-65% θ), and severe water deficit (W3, 45%-55% θ)] and four nitrogen application levels [no nitrogen application (N0, 0 kg·ha), low nitrogen application (N1, 150 kg·ha), medium nitrogen application (N2, 300 kg·ha), and high nitrogen application (N3, 450 kg·ha)] on nitrogen uptake by wolfberry plants, soil nitrogen loss, plant-soil nitrogen balance, and nitrogen use efficiency. The results indicate that: (1) Plant dry matter yield (1338.

IHNV induced miR-19-3p modulates immune response of rainbow trout (Oncorhynchus mykiss) by targeting DHX58-dependent RLR signaling pathway.

miR-19-3p has been implicated in various pathological and physiological processes, including immune response, inflammation, oncogenesis and cell damage. However, its function in rainbow trout (Oncorhynchus mykiss) has not been well elucidated. In this study, the expression patterns of miR-19-3p and target gene DExH-Box helicase 58 (DHX58) in rainbow trout infected with infectious hematopoietic necrosis virus (IHNV) were detected, and regulatory mechanism and function of miR-19-3p were investigated by overexpression and inhibition experiment in vitro and in vivo.

Melatonin modulated GPX5 and PTGDS expression in Bactrian camel epididymis mainly via receptor MT1.

Melatonin (Mel), an important mediator of photoperiodic annual rhythm regulation and seasonal reproduction in animals, directly modulates the expression of specific genes in the epididymis and protects sperm from oxidative damage. Bactrian camel is a dominant species in desert and semi-desert areas, exhibiting the unique reproductive regulation patterns. However, the underlying regulation mechanism of Mel on Bactrian camel is still unclear.

Development of a nanobody-based competitive enzyme-linked immunosorbent assay for the sensitive detection of antibodies against porcine deltacoronavirus.

Unlabelled: Porcine deltacoronavirus (PDCoV) is an emerging porcine enteric coronavirus causing significant economic losses to the pig farming industry globally. In this study, we expressed the S protein of a highly virulent PDCoV strain in the CHO eukaryotic expression system. After immunizing alpaca with the PDCoV S protein and employing the phage display library technique, a high-affinity and specific nanobody Nb3 against PDCoV S protein was successfully established by three rounds of biopanning and a phage enzyme-linked immunosorbent assay (ELISA).

Genome-wide analysis of non-coding RNA reveals the role of a novel miR319c for tuber dormancy release process in potato.

Tuber dormancy and sprouting are significant for potato cultivation, storage, and processing. Although the substantial role of microRNAs (miRNAs) in some biological processes has been recognized, the critical role of miRNA in breaking potato tuber dormancy is not well understood to date. In this investigation, we expand research on miRNA-mediated gene regulation in tuber dormancy release.

Effect of potato glycoside alkaloids on mitochondria energy metabolism of , the root rot pathogen of .

is a widely distributed pathogenic fungus that can cause soil borne diseases in various plants and is also one of the main pathogenic bacteria of root rot. This study employed tandem mass labeling (TMT) quantitative proteomics technology to investigate the antifungal mechanism of potato glycoside alkaloids (PGA) against . We elucidated the antifungal mechanism of PGA from the perspective of mitochondrial proteome molecular biology.

Rapamycin Increases the Development Competence of Yak () Oocytes by Promoting Autophagy via Upregulating 17β-Estradiol and HIF-1α During In Vitro Maturation.

High-quality oocyte production strategies play an important role in animal-assisted reproductive biotechnologies, and rapamycin (Rap) has been commonly used to increase the development potential of mammalian oocytes. The purpose of this study is to evaluate the effects and possible molecular mechanisms of rap on the maturation of yak oocytes. Different concentrations of Rap were supplemented during in vitro maturation (IVM) of yak oocytes.

Genetic Basis of Seedling Root Traits in Common Wheat ( L.) Identified by Genome-Wide Linkage Mapping.

Common wheat production is significantly influenced by abiotic stresses. Identifying the genetic loci for seedling root traits and developing the available molecular markers are crucial for breeding high yielding and stable varieties. In this study, five wheat seedling root traits, including root length (RL), root surface area (RA), root volume (RV), number of root tips (RT), and root dry weight (RW), were measured in the Wp-072/Wp-119 recombinant inbred line (RIL) population.

Innovative Utilization of Citrus Sinensis Peel Hydrogels: Enhancing Soil Water Retention and Efficient Removal of Methylene Blue from Wastewater.

In the context of increasing water scarcity and environmental pollution, this study investigates the synthesis and application of p(AA-Oco-AAm)-g-Citrus Sinensis Peel hydrogel (CSP hydrogel) to enhance soil water retention and remove organic dyes from wastewater. Hydrogels were prepared using a combination of acrylamide and acrylic acid, with the incorporation of citrus peel as a natural resource. The water absorption capacity of the hydrogels was evaluated, achieving a maximum retention rate of 477 g/g, significantly improving the water-holding ability of various soil types.

016 Alleviates Mice Colitis by Modulating Oxidative Stress, Gut Microbiota, and Microbial Metabolism.

Ulcerative colitis (UC) is a chronic and progressive inflammatory gastrointestinal disease closely associated with gut microbiota dysbiosis and metabolic homeostasis disruption. Although targeted microbial therapies are an emerging intervention strategy for inflammatory bowel disease (IBD), the mechanisms by which specific probiotics, such as 016 (LF), alleviate UC remain unclear. The current study evaluated the effects of LF supplementation on gut health in a basal model using C57BL/6 mice.

Effects of exogenous melatonin on the growth and photosynthetic characteristics of tomato seedlings under saline-alkali stress.

Saline-alkali stress is a major abiotic stress factor that adversely affects the growth, development, and yield of crops by disrupting ion homeostasis, osmotic balance, and metabolic processes. This study was designed to explore the alleviating effect of melatonin on the growth and development of tomato plants under saline-alkali stress conditions and to screen for optimal concentrations to alleviate the stress. Tomato variety 'Condine Red' was used as the test material, and a total of six treatments were designed including no saline-alkali stress and no melatonin spray as control (CK), and foliar spraying of 0, 50, 100, 150, and 200 µmol·L melatonin under saline-alkali stress (75 mmol·L), which were used to determine the growth and photosynthetic characteristics of tomato plants.

Revealing the impact of organic selenium-enriched Lactiplantibacillus plantarum NML21 on yogurt quality through volatile flavor compounds and untargeted metabolomics.

Effective selenium supplementation strategies are essential for alleviating global selenium deficiency. This study utilized the high‑selenium-tolerant strain Lactiplantibacillus plantarum NML21 to produce selenium-enriched yogurt, successfully converting inorganic Se(IV) into organic selenium, with selenoproteins accounting for 69.52 % of the total selenium content (995.

New BODIPY-Labeled Antibody for Detection of Foot-and-Mouth Disease Virus.

Detecting complete viral particles in vaccines is essential for both monitoring vaccine production and ensuring vaccine quality. Currently, sucrose density gradient centrifugation is the standard method for measuring the antigen content in conventional inactivated vaccines, but it requires specialized equipment and skilled personnel. Recently, BODIPY (BDP) dyes have gained attention in biomolecular studies due to their modifiable parent structure, although their use in macromolecular proteins remains limited.

Substitutability evaluation and resource utilization of non-medicinal parts of Ardisia crenata Sims, a traditional ethnic medicine.

The severe scarcity of wild resources of Ardisia crenata Sims (AS) has greatly limited its current clinical and pharmaceutical applications. We aimed to demonstrate the substitutability of stems and leaves for AS roots. In this study, 28 local samples from the Guizhou Province of China were selected for a comprehensive comparison of quality markers, chemical composition and efficacy.

Identification of ARF gene family and functional analysis of CqARF05 under drought and salt stress in quinoa.

The ARF gene family is crucial for regulating plant growth, development, and responses to various signaling pathways. In this study, 26 quinoa ARF genes (CqARF01-CqARF26) were identified, with encoded proteins varying in length from 553 to 1092 amino acids, molecular weights from 60.66 to 111.

Mechanisms of carbon and nitrogen distribution in soil aggregates in response to soil conditioners.

Soil conditioner has good performance in improving soil environment and solving soil barrier problems. The purpose of this study was to analyze the effects of different types of soil conditioners on the distribution of soil aggregates and the yield of corn, and to find out the suitable soil conditioners for improving the barren soil in the Yellow River irrigation area. The treatments include no soil conditioner (CK), cow dung organic fertilizer soil conditioner with different organic matter contents (Om-20%, Om-30%, Om-40%, Om-45%), humic acid soil conditioner (Hu-20%) and amino acid soil conditioner (Am-25%).

Impact of the eastern Tibetan Plateau on the ecological sensitivity of the West Qinling Mountains.

To investigate the mutual influence and interaction mechanisms between the Tibetan Plateau and the West Qinling Mountains, this study selects Zhuoni County, Lintan County, and Tanchang County as representative areas of the combined zone between the two geographic units. Additionally, Maqu County, characterized by the geomorphological features of the Tibetan Plateau, and Cheng County in the West Qinling Mountains are chosen as control areas. Based on the quantitative construction of water and soil erosion sensitivity, ecological risk sensitivity, land desertification sensitivity, and biodiversity sensitivity, this study comprehensively evaluated the ecological sensitivity of five typical counties using the equal weight superposition method.

[Research progress on variety breeding of root- and rhizome-derived traditional Chinese medicine].

Germplasm degeneration occurs during the long-term cultivation of root-and rhizome-derived traditional Chinese medicine(RR-TCM), which seriously restricts the high-quality development of their industry. Therefore, it is urgent to solve the problem of germplasm degeneration through variety breeding. In this paper, based on previously published research articles, monographs, and news reports, the research progresses on the number and origins, breeding methods, and selection of new varieties of RR-TCM listed in the Chinese Pharmacopoeia(Edition 2020) were summarized and analyzed.

Constructing a Novel Disease Resistance Mechanism Model for Cruciferous Crops: An Example From Black Rot.

Cruciferous crops are essential components of global agricultural production due to their rich nutritional value and extensive economic benefits. Black rot caused by Xanthomonas campestris pv. campestris (Xcc) has caused significant losses to cruciferous crops.

Astragalus polysaccharide inhibits infectious hematopoietic necrosis virus damage to rainbow trout (Oncorhynchus mykiss) spleen by promoting the efficacy of inactivated vaccine.

Pandemic infectious hematopoietic necrosis (IHN) caused by severe acute IHN virus (IHNV) threatens rainbow trout aquaculture in China. Therefore, it is crucial to develop effective vaccines and elucidate their mechanisms of action. Here, we revealed the mechanism of immune response of Astragalus polysaccharide (APS) combined with IHNV inactivated vaccine on rainbow trout spleen by serum enzyme activity assay, histopathological analysis, RNA-seq and viral load measurement.

Novel insights into the unique characterization of N6-methyladenosine RNA modification and regulating cold tolerance in winter Brassica rapa.

N6-methyladenosine (mA) is an mRNA modification considered essential in plants, and is a key player in gene regulation at the transcriptional and translational levels. In present study, we mapped Brassica rapa's whole transcriptome mA profile under low-temperature stress in different cold tolerant varieties to elucidate the mA methylation pattern. The distribution of mA modifications changed significantly under low temperature stress.

Transcriptomic and metabolomic analyses reveal the mechanism of color difference between two kinds of before and after drying.

Introduction: Cistanche deserticola is an important traditional Chinese herbal medicine. The fresh cistanche squamous stem is typically yellow-white and brown after drying. Oil cistanche is a cistanche variant with a purple squamous stem that turns black after drying.

Effects of dietary l-carnosine supplementation on the growth, intestinal microbiota, and serum metabolome of fattening lambs.

Dietary l-carnosine supplementation has been shown to enhance animal performance and improve meat quality. However, the mechanisms underlying the effects of l-carnosine on the physiological functions of animals have not been fully elucidated. We investigated the effects of dietary l-carnosine supplementation on growth performance, intestinal microbiota diversity, and the serum metabolome in fattening lambs to reveal the molecular mechanism underlying the effect of l-carnosine on the growth performance of sheep.

Ecosystem services value for water provisioning in the Shule River Basin of northwestern China: Spatial and temporal evolution and drivers from 2000 to 2022.

The economic and social functions of water provision have become increasingly important with the gradual scarcity of water resources due to global change. We assessed the spatial and temporal dynamics of the value of ecosystem water-provisioning services in terms of the monetary value shown in the Shule River Basin from 2000 to 2022 to sustain the structure, functionality, and stability of ecosystems. This provides an important reference for the optimization of ecosystem services in the basin and the scientific allocation of water resources.

Enhanced Quantum Efficiency in CaNaGdMgWO:Mn Phosphors: Allosteric Substitution to Disrupt Local Symmetry for Plant Cultivation Application.

Light-converting films enhance photosynthesis, yet most phosphors' low quantum efficiency restricts their application. This work finds that the co-substitution of Na and Gd for Ca in CaMgWO:Mn(CMWO: Mn) significantly enhances the deep red emission by nearly tenfold. The phosphor can emit red light with a peak at 688 nm under near-ultraviolet excitation, and an external quantum efficiency of 51.