Quality control of traditional Chinese medicines: a review.

Traditional Chinese medicines (TCMs) are in great demand all over the world, especially in the developing world, for primary health care due to their superior merits such as low cost, minimal side effects, better cultural acceptability, and compatibility with humans. However, Chinese medicines consist of several herbs which may contain tens, hundreds, or even thousands of constituents. How these constituents interact with each other, and what the special active ones are, may be the biggest bottleneck for the modernization and globalization of TCMs.

[Research on the compatibility laws in treating rheumatoid arthritis patients of cold-dampness obstruction syndrome].

Objective: To find out Chinese herbal compounds (CHCs) with high frequency in treating rheumatoid arthritis (RA) patients of cold-dampness obstruction syndrome (CDOS) by inductively reviewing literatures concerning clinical application of CHCs, thus improving theoretical and medical practice levels of Chinese medical recipes.

Methods: The CHCs for treating RA patients of CDOS published in medical journals in recent 30 years were retrieved. The database of CHCs was set up.

Simultaneous determination of phenylethanoid glycosides and aglycones by capillary zone electrophoresis with running buffer modifier.

Although the separation efficiency of capillary electrophoresis (CE) is much higher than that of other chromatographic methods, it is sometimes difficult to adequately separate the complex ingredients in biological samples. This article describes how one effective and simple way to develop the separation efficiency in CE is to add some modifiers to the running buffer. The suitable running buffer modifier β-cyclodextrin (β-CD) was explored to fast and completely separate four phenylethanoid glycosides and aglycones (homovanillyl alcohol, hydroxytyrosol, 3,4-dimethoxycinnamic acid, and caffeic acid) in Lamiophlomis rotata (Lr) and Cistanche by capillary zone electrophoresis with ultraviolet (UV) detection.

Profiling of hla-B alleles for association studies with ankylosing spondylitis in the chinese population.

Human leucocyte antigen (HLA) B*27 is a susceptibility allele to ankylosing spondylitis (AS). However, major AS-associated subtypes of HLA-B*27 and other HLA-B alleles vary in different ethnic populations. Herein, we examined HLA-B alleles in a total of 360 AS patients and 350 controls of Chinese Han ancestry.

[Effects of Gansu Dangshen decoction on the immune function of D-galactose induced aging mice].

Objective: To study the effect of Chinese herb Dangshen decoction on the immune function of D-galactose-induced aging mice.

Methods: A total of 60 mice were randomly divided into 5 groups, namely normal control group, aging model group, high and low concentration Dangshen treated groups, vitamin E control group. Aging mice models were made by D-galactose.

[Ultrafiltration membrane extract mixture from Angelica sinensis and Hedysarum polybotrys induced transdifferentiation of BMSCs in mice: an experimental research].

Objective: To observe and evaluate the effect of transdifferentiation of bone marrow derived stroma cells (BMSCs) into nerve cells by ultrafiltration membrane extract mixture from Angelica sinensis and Hedysarum polybotrys.

Methods: The BMSCs in vitro cultured after treated by ultrafiltration membrane extract mixture from Angelica sinensis and Hedysarum polybotrys were divided into 5 groups, i.e.

[Effects of conjunctive application of Chinese herbal medicine residue compost and chemical fertilizer on ferulic acid and ligustilide contents in Angelica sinensis].

Objective: To investigate the effects of conjunctive application of Chinese herbal medicine residue compost (CHMRC) and chemical fertilizer on ferulic acid and ligustilide contents in Angelica sinensis.

Methods: There were six treatments, viz. T1: control (no fertilizer), T2: chemical fertilizer (the fertilizer rates for N, P2O5 and K2O were 90, 90 and 60 kg/hm2, respectively), T3:1 350 kg/hm2 CHMRC, 86 kg/hm2N, 84 kg/hm2 P2O5 and 52 kg/hm2 K2O, T4:2 250 kg/hm2 CHMRC, 83 kg/hm2N, 81 kg/hm2 P2O5 and 47 kg/hm2 K2O, T5: 3 150 kg/hm2 CHMRC, 82 kg/hm2N, 77 kg/hm2 P2O5 and 42 kg/hm2 K2O, and T6: CHMRC at 4 500 kg/hm2 (nitrogen application rate was the same as T2).

[Comparison of content of total alkaloids and aconitine in Aconitum szechenyianum from different producing areas on basis of macroporous adsorption resin purification technology].

Objective: To optimize the conditions of purifying the total alkaloids in Aconitum szechenyianum with macroporous adsorption resin, and compare the content of total alkaloids and aconitine in A. szechenyianum from different producing areas, in order to provide basis for further studies.

Method: The orthogonal experiment method was adopted for optimizing the conditions of purifying the total alkaloids in A.

STAT4 is a genetic risk factor for systemic sclerosis in a Chinese population.

Systemic sclerosis (SSc) is an immune-mediated and complex genetic disease. An association of single-nucleotide polymorphisms (SNPs) in the STAT4 gene with SSc has been reported in European Caucasians, North Americans and Japanese. We undertook the current study to examine whether the STAT4 SNPs are also associated with susceptibility to SSc and SSc subsets in a Han Chinese population.

[Effects of guipi pill on bone marrow cell cycle of mice exposed to benzene].

Objective: To observe the effects of Guipi Pill (GPP) on bone marrow cell cycle of mice exposed to benzene and to explore its possible mechanisms for regulating hematopoiesis.

Methods: Seventy-two Kunming mice were randomly divided into 6 groups, i.e.

[Study on the dynamic variations of Z-ligustilide and n-butylidenephthalide content in essential oil of radix angelicae sinensis from different growth period].

Objective: To study the variation of the biomass of the root and active components of Angelica sinensis during different growth periods.

Methods: 27 batches of Angelica sinensis were harvested from different growth periods, and the biomass of underground parts were determined; The Gas Chromatography-Mass Spectrometry (GC-MS) was used for determining the contents of Z-ligustilide and n-Butylidenephthalide in essential oil of Radix Angelicae Sinensis.

Results: The average contents of n-Butylidenephthalide and Z-ligustilide were more than 1% and 40% in the total essential oil of Radix Angelicae sinensis respectively.

[Preparation technology and quantitative analysis for beta-cyclodextrin inclusion compound of Angelica oil].

Objective: To optimize the preparation process of beta-cyclodextrin (beta-CD) inclusion compound of Angelica oil.

Methods: Single factor experiment and orthogonal design were used together, oil inclusion rate was used as an index, then chose the best preparation technology.

Results: The optimal method was saturated solution method.

[Effect of altitudes on the photosynthate accumulation and distribution pattern of Angelic sinensis].

Objective: To study the effect of altitudes on the photosynthate accumulation and distribution pattern of Angelic sinensis in Gansu province and provide theontical for its expanding ecological planting region.

Methods: Used field test to study the photosynthate accumulation and distribution pattern of Angelic sinensis on three different altitudinal gradients from 2 300 m to 2 800 m.

Results: Before September 25, total photosynthate accumulation were decreased with the increasing of elevation, which amaunt was 176 g/plant, 166 g/plant and 128 g/plant, respectively.

[Relationship between seedling grade of angelica and biomass accumulation and quality of product].

Objective: To research the relationship between the seedling grade of angelica and the biomass accumulation, output and quality of product, so as to provide base for establishing seedling standard.

Method: Thirty seedlings of Angelica were collected from the main production area in Gansu province, such as Minxian, Zahngxian, Dangchang and Weiyuan county, all the samples were measured with weigh of single seedling and the seedling were divided into three grade by the clustering analysis results, the grade were made to treatment to do field test and laboratory experiment.

Result: The weigh of dry root, above ground and the whole plant in growth period of treatment 2 (the weigh of single seedling between 0.

Progress in the clinical imaging research of bone diseases on ankle and foot sesamoid bones and accessory ossicles.

Sesamoid bones and accessory ossicles are research focuses of foot and ankle surgery. Pains of the foot and ankle are related to sesamoid bones and accessory ossicles. The specific anatomical and functional relationship of sesamoid bones and accessory ossicles can cause such bone diseases as the dislocation of sesamoid bones and accessory bones, infection, inflammation and necrosis of sesamoid bones, cartilage softening, tenosynovitis of sesamoid bones and the sesamoid bone syndrome.

[Effect of pingxian granules on protein expression of apoptosis regulatory genes in hippocampus of pentylenetetrazol-induced epileptic model rats].

Objective: To observe the effect of Pingxian granules on the protein expression of apoptosis regulatory genes Bcl-2 and Bax in the hippocampus of epileptic model rats and study the molecular biological mechanism of the anti-epileptic effect of Pingxian granules.

Method: Totally 60 45-days-old Wistar rats were selected and then randomly assigned into 5 groups: the normal control group, the model group, the positive control group, the Pingxian high dose group and the Pingxian low dose group, with 12 in each group. Except the normal control group, all the groups were intraperitoneally injected with 35 mg x kg(-1) pentylenetetrazol to establish the models of epilepsy.

[Physiological and biochemical changes of angelica seeds during storage with different moisture contents].

Objective: To study the physiological and biochemical changes during storage of angelica seeds with different moisture contents.

Method: The dynamic changes of percentage and index of seeds germination, relative conductivity, superoxide dismutase (SOD) activity, contents of soluble sugar and protein were determined at an interval of every two months.

Result: Under the conditions stored in sealed paper cups at 15 degrees C for 10 months, the germination percentage of angelica seeds with 2.

[Study on traditional Chinese medicine prescriptions for cardiovascular diseases over the past 20 years and logistic regression analysis].

Objective: To analyze the relationship between TCM's characteristics and treatment of cardiovascular diseases on the basis of the modem medical research literatures about treatment of cardiovascular diseases and establish a mathematic model in order to give reference for clinical study and clinical prescription for treatment of cardiovascular diseases with TCMs.

Method: Articles on treatment of cardiovascular diseases with TCMs published at home in the past 20 years were collected and screened to summarize the number of TCMs with different function, property, flavor, channel tropism and the number of them with the therapeutically effect on cardiovascular diseases. And a mathematic model was established on the multivariate discriminatory analysis.

N-Succinyl-chitosan nanoparticles induced mitochondria-dependent apoptosis in K562.

N-Succinyl-chitosan nanoparticles (NSCNP) are synthetical nanoparticles derived from chitosan. In our previous study, we demonstrated that NSCNP could induce apoptosis in K562 cells, which were evidenced by decreasing cell zeta potential, disrupting the mitochondrial membrane potential, increasing ROS generation and Ca2+ concentration. Here, by western blot we revealed that apoptosis induced by NSCNP was associated with accumulation of cytochrome c in cytosol and elevating expression of apoptosis-promoted protein Bax while depressing expression of apoptosis-restrained protein Bcl-2 in time- and dose-dependence.

[Determination of o-methylflavinantine in a Tibetan medicine Meconopsis quintuplinervia by HPLC].

Objective: To develop an HPLC method for the determination of a Tibetan medicine Meconopsis quintuplinervia.

Method: A Hypersil-Keystone-C18 column (4.6 mm x 250 mm, 5 microm) was used with the isocratic elution of acetonitrile and 0.

[Study on semi-synthetic transforming technology for the natural product of isocorydione].

Transforming technology for semi-synthesized isocorydione from the natural product ofisocorydine was studied. The factors affecting on the reaction yield were investigated. UV spectrophotometry was used to indicate the semi-synthesized yield of isocorydione.

The influence of the total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21Ras and proliferating cell nuclear antigen gene in erythroleukemia cell line K562.

Objective: To investigate the effect of total flavonoids of Hedysarum polybotry on the proliferation, cell cycle, and expressions of p21(Ras) and proliferating cell nuclear antigen (PCNA) gene in erythroleukemia cell line K562.

Methods: The effect of total flavonoids of Hedysarum polybotry on K562 cell line survival was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) reduction assay. The time- and dose-dependent manner was also observed.

[Influences of different initial processing methods on Rheum palmatum].

Objective: To choose the optimum initial processing methods of Rheum palmatum.

Methods: Fresh crude Rheum palmatum was sliced and dealt with the different drying methods such as sun drying, shady drying, microwave heating and various temperatures drying. The content of the Anthraquinones derivatives, slicing colors and dried rates were used as evaluation indexes.

ASYMMETRIC LEAVES2 gene, a member of LOB/AS2 family of Arabidopsis thaliana, causes an abaxializing leaves in transgenic cockscomb.

The leaf primordium derives from the peripheral zone of shoot apical meristem. During the formation of leaf primordia, they need to establish the proximodistal, mediolateral, and ab/adaxial axes. Among these axes, the ab/adaxial axis might be the most important.

Protection of ultra-filtration extract from Danggui Buxue Decoction on oxidative damage in cardiomyocytes of neonatal rats and its mechanism.

Objective: To investigate whether the administration of the ultra-filtration extract from Danggui Buxue Decoction (EDBD) was able to protect cardiomyocytes from oxidative injury of rats induced by hydrogen peroxide (H(2)O(2)) and its potential mechanism.

Methods: Myocardial cells from 1- to 2-day-old neonatal rats were cultured in Dulbecco's modified Eagle's medium low-glucose and Ham's F12 medium (1:1), and the cellular injury was induced by H(2)O(2). The ultra-filtration extract mixture from Angelica sinensis and Hedysarum polybotrys was given in three doses of 3.