135 results match your criteria: "GSF Research Center[Affiliation]"
Oncogene
August 2024
Department of Oncology/Hematology, Dr von Haunersches Kinderspital, München, Germany.
Front Biosci
May 2008
Clinical Cooperation Group Molecular Oncology, GSF-Research Center for Environment and Health, and Head and Neck Research Dept. Munich, Germany.
Epithelial cell adhesion molecule EpCAM is strongly over-expressed in a variety of carcinomas where it is involved in signalling events resulting in increased expression of target genes such as c-Myc, cyclins and others, eventually conferring cells an oncogenic phenotype. However, EpCAM is also expressed in a series of healthy epithelia, albeit generally to a far lesser extend. We have uncovered differential glycosylation of EpCAM as a means to discriminate normal from malignant tissues.
View Article and Find Full Text PDFHandb Exp Pharmacol
August 2008
GSF - Research Center for Environment and Health, Institute of Toxicology, Ingolstädter Landstr. 1, Neuherberg, Germany.
NF-kappaB is a major regulator of the first-line defense against invading pathogens, antigen-specific adaptive immune responses or chemical stress. Stimulation either by extracellular ligands (e.g.
View Article and Find Full Text PDFMethods Mol Biol
January 2009
MIPS Institute for Bioinformatics, GSF Research Center for Environment and Health, Neuherberg, Germany.
The Munich Institute for Protein Sequences (MIPS) has been involved in maintaining plant genome databases since the Arabidopsis thaliana genome project. Genome databases and analysis resources have focused on individual genomes and aim to provide flexible and maintainable data sets for model plant genomes as a backbone against which experimental data, for example from high-throughput functional genomics, can be organized and evaluated. In addition, model genomes also form a scaffold for comparative genomics, and much can be learned from genome-wide evolutionary studies.
View Article and Find Full Text PDFEur Spine J
March 2008
Institute of Pathology, Division of Neuropathology, Technical University, Ismaninger Strasse 22, 81675, Munich, Germany.
Genetic factors seem to play a role in symptomatic lumbar disc disease (LDD). It has been shown previously that a tryptophan mutation of the COL9A2 gene is a major risk factor for LDD in a Finish population. The impact of collagen gene variations on the relapse rate after lumbar discectomy, however, has not been studied so far.
View Article and Find Full Text PDFScience
December 2007
Institute of Clinical Molecular Biology and Tumour Genetics, GSF-Research Center of Environment and Health, Munich Center for Integrated Protein Science (CiPSM), Marchioninistrasse 25, 81377 Munich, Germany.
RNA polymerase II is distinguished by its large carboxyl-terminal repeat domain (CTD), composed of repeats of the consensus heptapeptide Tyr1-Ser2-Pro3-Thr4-Ser5-Pro6-Ser7. Differential phosphorylation of serine-2 and serine-5 at the 5' and 3' regions of genes appears to coordinate the localization of transcription and RNA processing factors to the elongating polymerase complex. Using monoclonal antibodies, we reveal serine-7 phosphorylation on transcribed genes.
View Article and Find Full Text PDFMol Cell Proteomics
February 2008
Institute of Toxicology, GSF-Research Center for Environment and Health, D-85764 Neuherberg, Germany.
As part of a comprehensive survey of the impact of the environmental pollutant and hepatocarcinogen 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the proteome of hepatic cells, we have performed a high resolution two-dimensional gel electrophoresis study on the rat hepatoma cell line 5L. 78 protein species corresponding to 73 different proteins were identified as up- or down-regulated following exposure of the cells to 1 nm TCDD for 8 h. There was an overlap of only nine proteins with those detected as altered by TCDD in our recent study using the non-gel-based isotope-coded protein label method (Sarioglu, H.
View Article and Find Full Text PDFAnn Hematol
March 2008
Clinical Cooperation Group Gene Therapy, GSF-Research Center for Environment and Health, Marchioninistrasse 25, 81377, Munich, Germany.
The constitutive tyrosine kinase activity of the BCR-ABL fusion protein plays a crucial role in the pathogenesis of chronic myeloid leukemia and promotes growth factor-independent survival of hematopoietic cells. In 32D cells, expression levels of retrovirally transduced BCR-ABL were positively correlated with the levels of the cell cycle regulator protein p21, and this upregulation of p21 expression depended on the kinase activity of BCR-ABL. To assess the role of p21 on BCR-ABL-positive hematopoietic cells, we compared proliferation and drug-induced apoptosis in bone marrow (BM) cells from wild-type and p21 knockout mice after retroviral transfer of the BCR-ABL fusion gene.
View Article and Find Full Text PDFAnal Chem
November 2007
Institute of Groundwater Ecology, GSF-Research Center for Environment and Health, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
Potentially, compound-specific isotope analysis may provide unique information on source and fate of pesticides in natural systems. Yet for isotope analysis, LC-based methods that are based on the use of organic solvents often cannot be used and GC-based analysis is frequently not possible due to thermolability of the analyte. A typical example of a compound with such properties is isoproturon (3-(4-isopropylphenyl)-1,1-dimethylurea), belonging to the worldwide extensively used phenylurea herbicides.
View Article and Find Full Text PDFAnal Bioanal Chem
November 2007
GSF Research Center for Environment and Health, Institute of Ecological Chemistry, Ingolstädter Landstrasse 1, 85764 Neuherberg, Germany.
This perspective article provides an assessment of the state-of-the-art in the molecular-resolution analysis of complex organic materials. These materials can be divided into biomolecules in complex mixtures (which are amenable to successful separation into unambiguously defined molecular fractions) and complex nonrepetitive materials (which cannot be purified in the conventional sense because they are even more intricate). Molecular-level analyses of these complex systems critically depend on the integrated use of high-performance separation, high-resolution organic structural spectroscopy and mathematical data treatment.
View Article and Find Full Text PDFEnviron Res
May 2008
Institute of Toxicology, GSF Research Center for Environment and Health, Munich, Germany.
Unlabelled: Parts of the population are permanently exposed to low levels of Hg degrees and Hg(II) from dental amalgam. It was the aim (1) to investigate the internal exposure to amalgam-related mercury from the kinetics of inorganic Hg in plasma and erythrocytes after amalgam removal, and (2) to estimate the amalgam-related absorbed dose. Dietary coexposure was monitored by determination of blood organic-Hg.
View Article and Find Full Text PDFBMC Bioinformatics
August 2007
MIPS/Institute for Bioinformatics, GSF Research Center for Environment and Health, Ingolstädter Landstrasse 1; 85764 Neuherberg, Germany.
Background: Apollo, a genome annotation viewer and editor, has become a widely used genome annotation and visualization tool for distributed genome annotation projects. When using Apollo for annotation, database updates are carried out by uploading intermediate annotation files into the respective database. This non-direct database upload is laborious and evokes problems of data synchronicity.
View Article and Find Full Text PDFToxicol Pathol
April 2007
GSF Research Center for Environment and Health, Institute of Pathology, Neuherberg 85764, Germany.
Immunohistochemistry is an indispensable tool in human pathology enabling immunophenotypic characterization of tumor cells. Immunohistochemical analyses of mouse models of human hematopoietic neoplasias have become an important aspect for comparison of murine entities with their human counterparts. The aim of this study was to establish a diagnostic antibody panel for analysis of murine lymphomas/leukemias, useful in formalin-fixed/paraffin-embedded tissue.
View Article and Find Full Text PDFMol Cell Biol
May 2007
Institute of Clinical Molecular Biology and Tumor Genetics, GSF Research Center, Marchioninistrasse 25, D81377 Munich, Germany.
The PeBoW complex is essential for cell proliferation and maturation of the large ribosomal subunit in mammalian cells. Here we examined the role of PeBoW-specific proteins Pes1, Bop1, and WDR12 in complex assembly and stability, nucleolar transport, and pre-ribosome association. Recombinant expression of the three subunits is sufficient for complex formation.
View Article and Find Full Text PDFGenesis
January 2007
Department of Comparative Medicine, GSF Research Center for Environment and Health, Neuherberg, Germany.
Inducible and tissue-specific gene inactivation in mice has become a powerful tool to bypass embryonic and postnatal lethality of knockout mice. The most frequently used inducible system is based on Cre recombinase fused to either one or two mutated estrogen receptor ligand binding domains, thus rendering Cre function tamoxifen-dependent. To achieve Cre-mediated inactivation of a given gene, 4-OH tamoxifen (4-OHT) dissolved either in alcohol and/or oil is usually administered by repeated intraperitoneal (i.
View Article and Find Full Text PDFNucleic Acids Res
January 2007
MIPS, Institute for Bioinformatics, GSF Research Center for Environment and Health, Ingolstädter Landstr. 1 85764 Neuherberg, Germany.
Genome-oriented plant research delivers rapidly increasing amount of plant genome data. Comprehensive and structured information resources are required to structure and communicate genome and associated analytical data for model organisms as well as for crops. The increase in available plant genomic data enables powerful comparative analysis and integrative approaches.
View Article and Find Full Text PDFGenetics
March 2007
Institute of Experimental Genetics, GSF Research Center for Environment and Health, 85764 Neuherberg, Germany.
The Notch signaling pathway is an evolutionarily conserved transduction pathway involved in embryonic patterning and regulation of cell fates during development. Recent studies have demonstrated that this pathway is integral to a complex system of interactions, which are also involved in distinct human diseases. Delta1 is one of the known ligands of the Notch receptors.
View Article and Find Full Text PDFNucleic Acids Res
March 2007
Institute of Clinical Molecular Biology and Tumour Genetics, GSF Research Center, Marchioninistrasse 25, 81377 Munich, Germany.
RNA interference (RNAi) is a powerful tool to analyze gene function in mammalian cells. However, the interpretation of RNAi knock-down phenotypes can be hampered by off-target effects or compound phenotypes, as many proteins combine multiple functions within one molecule and coordinate the assembly of multimolecular complexes. Replacing the endogenous protein with ectopic wild-type or mutant forms can exclude off-target effects, preserve complexes and unravel specific roles of domains or modifications.
View Article and Find Full Text PDFJ Stud Alcohol Drugs
January 2007
Institute of Epidemiology, GSF Research Center for Environment and Health, Ingolstaedter Landstrasse 1, D-85764 Neuherberg, Germany.
Objective: This study investigated differences regarding degree, duration, and motivation of abstinence within a group of individuals with very low alcohol consumption. Reliability of self-reported alcohol abstinence was examined.
Method: Interview data on alcohol intake and abstinence were investigated in 412 male and 377 female population-representative participants of the cross-sectional KORA/ MAGiC-Control study.
J Environ Radioact
February 2007
GSF-Research Center for Environment and Health, Institute of Radiation Protection, Ingolstädter Landstr. 1, 85764 Neuherberg, Germany.
This paper describes the long-term behaviour of (90)Sr and (137)Cs in foods, feeds and a variety of environmental media. The long-term behaviour is quantified by means of the ecological half-life which integrates all processes that cause a decrease of activity in a given medium such as leaching, fixation and erosion. A large number of long-term time series of concentrations of radiocaesium and radiostrontium in these media have been identified and re-evaluated using a standardised statistical procedure to establish reference data sets of ecological half-lives.
View Article and Find Full Text PDFGenome Res
October 2006
Munich Information Center for Protein Sequences (MIPS), Institute for Bioinformatics, GSF Research Center for Environment and Health, D-85764 Neuherberg, Germany.
Maize (Zea mays or corn), both a major food source and an important cytogenetic model, evolved from a tetraploid that arose about 4.8 million years ago (Mya). As a result, maize has extensive duplicated regions within its genome.
View Article and Find Full Text PDFPlant Cell Environ
August 2006
Institute of Biochemical Plant Pathology, GSF-Research Center for Environment and Health, Neuherberg, Germany.
The major UV-B screening pigments of the epidermal layer of Scots pine (Pinus sylvestris) needles are flavonol 3-o-glycosides (F3Gs) esterified with hydroxycinnamic acids at positions 3" and 6". Acylation is the last step in biosynthesis and is catalysed by position-specific hydroxycinnamoyl transferases (3" and 6"HCT). The UV-B dependence of these enzyme activities was studied in primary needles of Scots pine seedlings grown under different UV-B conditions in environmentally controlled sun simulators.
View Article and Find Full Text PDFBlood
December 2006
Institute of Clinical and Molecular Biology, GSF-Research Center for Environment and Health, Marchioninistrasse 25, 81377 München, Germany.
Somatic hypermutation and class-switch recombination in germinal centers critically depend on activation-induced cytidine deaminase (AID). Deregulation of AID may lead to the aberrant activation or persistence of both genetic processes, thus contributing to the pathogenesis of B-cell lymphomas by mistargeted mutagenesis or recombination. The Epstein-Barr virus (EBV) establishes an asymptomatic latent infection in more than 90% of the human population, but it has also been linked to lymphomagenesis.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
August 2006
Institute of Clinical Molecular Biology and Tumor Genetics, GSF-Research Center for Environment and Health, Marchioninistrasse 25, 81377 Munich, Germany.
Somatic hypermutation of Ig genes is initiated by transcription-coupled cytidine deamination in Ig loci. Error-prone processing of the resultant DNA lesions is thought to cause extensive mutagenesis, but it is presently an enigma how and why error-prone rather than error-free repair pathways are recruited. During DNA replication, recruitment of error-prone translesion polymerases may be mediated by Rad6/Rad18-mediated ubiquitination of proliferating cell nuclear antigen, a major switchboard controlling the fidelity of DNA lesion bypass in eukaryotes.
View Article and Find Full Text PDFJ Neurosci Methods
October 2006
GSF Research Center for Environment and Health, Institute of Experimental Genetics, D-85764 Neuherberg, Germany.
Neurological and psychiatric disorders are among the most common and most serious health problems in developed countries. Transgenic mouse models mimicking human neurological diseases have provided new insights into development and function of the nervous system. One of the prominent goals of the German National Genome Research Network is the understanding of the in vivo function of single genes and the pathophysiological and clinical consequences of respective mutations.
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