Draft Genome Sequences of Marinobacter similis A3d10T and Marinobacter salarius R9SW1T.

Here, we present the draft genomes of Marinobacter similis A3d10(T), a potential plastic biodegrader, and Marinobacter salarius R9SW1(T), isolated from radioactive waters. This genomic information will contribute information on the genetic basis of the metabolic pathways for the degradation of both plastic and radionuclides.

Determination of cucumarioside A₂-2 in mouse spleen by radiospectroscopy, MALDI-MS and MALDI-IMS.

The distribution of triterpene glycoside cucumarioside A₂-2, the main compound of medical lead Cumaside in immunodeficiency diseases, in mouse spleen was determined. For this purpose the stability and dynamics of glycoside content changes over time in Balb/c mouse spleen tissue homogenate as well as the study of the cucumarioside A2-2 spatial distribution in tissue sections were investigated using radiospectroscopy, MALDI-MS and MALDI Imaging Mass Spectrometry (IMS), correspondingly. Cucumarioside A₂-2 is reliably detected by MALDI-MS in the mouse spleen tissue after single intraperitoneal (i.

The structure of the O-specific polysaccharide from marine bacterium Litorimonas taeanensis G5T containing 2-acetamido-4-((3S,5S)-3,5-dihydroxyhexanamido)-2,4-dideoxy-D-quinovose and 2-acetamido-2,6-dideoxy-L-xylo-hexos-4-ulose.

The O-polysaccharide was isolated from the lipopolysaccharide of Litorimonas taeanensis G5(T) and studied by chemical methods along with (1)H and (13)C NMR spectroscopy, including (1)H, (1)H COSY, 1D and 2D TOCSY, NOESY, (1)H, (13)C HSQC, HMBC, and H2BC experiments. The following new structure of the O-polysaccharide of L. taeanensis G5(T) containing 2-acetamido-2-deoxy-D-galacturonic acid (D-GalNAcA), 2-acetamido-4-((3S,5S)-3,5-dihydroxyhexanamido)-2,4-dideoxy-D-quinovose (D-QuiNAc4NR), and 2-acetamido-2,6-dideoxy-L-xylo-hexos-4-ulose (L-Sug) was established: →4)-α-D-GalpNAcA-(1→3)-α-D-QuiNAc4NR-(1→3)-α-L-Sug-(1→ where R is (3S,5S)-3,5-dihydroxyhexanoic acid.

Regioselective benzoylation of glycopyranosides by benzoic anhydride in the presence of Cu(CF3COO)2.

Benzoylation of methyl and benzyl glycopyranosides by benzoic anhydride in acetonitrile in the presence of copper(II) trifluoroacetate as a promoter has given monobenzoates with a good yield and high regioselectivity. The composition of monobenzoates depended both on a configuration of hydroxyl groups and on a configuration of aglycone. The simple syntheses of the monobenzoates of some glycosides are offered.

ESIMS analysis of fucoidan preparations from Costaria costata, extracted from alga at different life-stages.

Four fucoidan fractions from brown alga Costaria costata, collected at different life-stages: vegetative, May (5F2 and 5F3) and generative, July (7F1 and 7F2) collections were characterized. It was found that seaweed synthesizes different set of fucoidans - one with high fucose content and substantial percentage of hexoses and uronic acid and lower sulfate content (7F1, 5F2 and 5F3) and other - highly sulfated galactofucan (7F2). Structural features of fractions 7F2 and 5F3 were predominantly determined by mass spectrometric analysis of low-molecular-weight (LMW) oligosaccharide fragments, obtained by autohydrolysis of 7F2 and mild acid hydrolysis of 5F3 fucoidans.

Structural similarities of fucoidans from brown algae Silvetia babingtonii and Fucus evanescens, determined by tandem MALDI-TOF mass spectrometry.

Rapid mass spectrometric investigation of oligosaccharides, obtained by autohydrolysis of fucoidans from brown algae Silvetia babingtonii and Fucus evanescens (Fucales, Phaeophyceae) has shown both similarities and differences in structural features/sulfation pattern of their fragments, obtained in the same conditions. Tandem MALDI-TOF MS of fucooligosaccharides with even DP (degree of polymerization) was close to that observed for fucoidan from F. evanescens.